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The map label for this gene is dadA [C]

Identifier: 92112627

GI number: 92112627

Start: 541123

End: 542250

Strand: Direct

Name: dadA [C]

Synonym: Csal_0494

Alternate gene names: 92112627

Gene position: 541123-542250 (Clockwise)

Preceding gene: 92112626

Following gene: 92112628

Centisome position: 14.64

GC content: 68.09

Gene sequence:

>1128_bases
ATGATAGATTGCGGGTTTCGCCAGCCAAGAACGAGTGTCCCTGTGAGTGAATTCTTGATCGTCGGGGGCGGCGTCATCGG
CATGATGACGGCCCTGCAACTCGCCGATGCCGGCCGCCGCGTCACCCTGCTCGAGCGCGGCGAGTGCGGCCGTGAGGCCT
CCTGGGCCGGCGGTGGCATCGTCTCCCCGTTGTATCCCTGGCGCTACAGCGCGCCGATTTCCACGTTGTCGCGTTATTCC
GAAGGGGCCTACCCCGAGTTGTCGCTGCGCCTGCTGGAGGAAACCGGCATCGACCCCGAATACCGCCAGCGGGGGCTCTT
GTACCTGCGTGTCGACGATGAAGAGCGGGCGCTCGACTGGGCGCGCCAGGAAGGCAAGCCCCTGCAGCGGGTCGGGCCGG
AGACGATCTACGCCAAGGAGCCCAATGCCGCGCCGGGCGTCGAGTCGGCGCTGTGGATGCCGACCCTGGGCAGCATCCGC
AATCCGCGCCTGTGTCGCGCGTTGCGTGCCCGCTTGCAGGCGATGCCCAACGTGGCGCTGCGCGAGCACGTCGATGTCGA
GGAACTGGTGGCATCGGCGGGACGCATCCAGGGGGTGCGCACCTCGGCGGGACACGAAACGGCCGAATGCGTGGTGGTAT
GTGGCGGTGCCTGGGCCAGCCAGCTGCTGGCGAGCGTCGATGTGGCGCTGCCCGTGCGCCCGGTGCGCGGGCAGATGATC
CTGTTCAAGGCGCCGCCGGGGCTGGTCGAGCGCGTGGTGTTGAAAGATGGTCGCTATGTGATTCCCCGGGGCGACGGGCG
TGTCGTCGCCGGTTCCACGCTCGAAGAGGTAGGATTCGACAAGCGTACCACCGAGGCCGCCAAGGGCTCTCTCTATGACA
GCGCGCTGTCCATCGTGCCGGGGCTGGCCGACTGCCCGGTGGAACATCACTGGGCGGGGTTGCGCCCCGGCTCGCCGGAT
GGGGTGCCCCGCATCGGTGCGGTACCGGGCGTCGAGGGCCTCTGGGTCAATGCCGGACACTATCGCAATGGCCTGGTGCT
GGCACCGGCGTCGACGCGTCTGCTGGCCGATCAGTTGCTGCAACGCACACCGGTCGTCGATCCCGCCCCTTATCGACTGG
ACACCTGA

Upstream 100 bases:

>100_bases
GCGTGCTGAAGTTGTACGATACCGGGCAAAAACAGCCAGAAAAATGCTGGTGACACATTGAGAGTCAAGCAATGGCAGGC
GCTGCCTTGCTGAGCGTCGC

Downstream 100 bases:

>100_bases
CGGCGGCACTTTTTGCCAGCGGGCCAGCCTTGCGTGACAATGCCGACATGCGGTCGTTAACACGGCCTGACTCCCTGCCA
ATCGCAATGAGAGAGGCGCT

Product: glycine oxidase ThiO

Products: FADH2; ammonia; Pyruvate [C]

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MIDCGFRQPRTSVPVSEFLIVGGGVIGMMTALQLADAGRRVTLLERGECGREASWAGGGIVSPLYPWRYSAPISTLSRYS
EGAYPELSLRLLEETGIDPEYRQRGLLYLRVDDEERALDWARQEGKPLQRVGPETIYAKEPNAAPGVESALWMPTLGSIR
NPRLCRALRARLQAMPNVALREHVDVEELVASAGRIQGVRTSAGHETAECVVVCGGAWASQLLASVDVALPVRPVRGQMI
LFKAPPGLVERVVLKDGRYVIPRGDGRVVAGSTLEEVGFDKRTTEAAKGSLYDSALSIVPGLADCPVEHHWAGLRPGSPD
GVPRIGAVPGVEGLWVNAGHYRNGLVLAPASTRLLADQLLQRTPVVDPAPYRLDT

Sequences:

>Translated_375_residues
MIDCGFRQPRTSVPVSEFLIVGGGVIGMMTALQLADAGRRVTLLERGECGREASWAGGGIVSPLYPWRYSAPISTLSRYS
EGAYPELSLRLLEETGIDPEYRQRGLLYLRVDDEERALDWARQEGKPLQRVGPETIYAKEPNAAPGVESALWMPTLGSIR
NPRLCRALRARLQAMPNVALREHVDVEELVASAGRIQGVRTSAGHETAECVVVCGGAWASQLLASVDVALPVRPVRGQMI
LFKAPPGLVERVVLKDGRYVIPRGDGRVVAGSTLEEVGFDKRTTEAAKGSLYDSALSIVPGLADCPVEHHWAGLRPGSPD
GVPRIGAVPGVEGLWVNAGHYRNGLVLAPASTRLLADQLLQRTPVVDPAPYRLDT
>Mature_375_residues
MIDCGFRQPRTSVPVSEFLIVGGGVIGMMTALQLADAGRRVTLLERGECGREASWAGGGIVSPLYPWRYSAPISTLSRYS
EGAYPELSLRLLEETGIDPEYRQRGLLYLRVDDEERALDWARQEGKPLQRVGPETIYAKEPNAAPGVESALWMPTLGSIR
NPRLCRALRARLQAMPNVALREHVDVEELVASAGRIQGVRTSAGHETAECVVVCGGAWASQLLASVDVALPVRPVRGQMI
LFKAPPGLVERVVLKDGRYVIPRGDGRVVAGSTLEEVGFDKRTTEAAKGSLYDSALSIVPGLADCPVEHHWAGLRPGSPD
GVPRIGAVPGVEGLWVNAGHYRNGLVLAPASTRLLADQLLQRTPVVDPAPYRLDT

Specific function: Oxidative Deamination Of D-Amino Acids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Inner Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DadA oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI1787438, Length=405, Percent_Identity=25.4320987654321, Blast_Score=93, Evalue=4e-20,
Organism=Drosophila melanogaster, GI20130091, Length=384, Percent_Identity=24.7395833333333, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28571104, Length=382, Percent_Identity=24.3455497382199, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076
- InterPro:   IPR012727 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.4.99.1 [C]

Molecular weight: Translated: 40519; Mature: 40519

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDCGFRQPRTSVPVSEFLIVGGGVIGMMTALQLADAGRRVTLLERGECGREASWAGGGI
CCCCCCCCCCCCCCHHHHEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
VSPLYPWRYSAPISTLSRYSEGAYPELSLRLLEETGIDPEYRQRGLLYLRVDDEERALDW
CCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHCCEEEEEECCHHHHHHH
ARQEGKPLQRVGPETIYAKEPNAAPGVESALWMPTLGSIRNPRLCRALRARLQAMPNVAL
HHHCCCCHHHCCCCEEEECCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
REHVDVEELVASAGRIQGVRTSAGHETAECVVVCGGAWASQLLASVDVALPVRPVRGQMI
HHCCCHHHHHHHHHHCCCEECCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCEE
LFKAPPGLVERVVLKDGRYVIPRGDGRVVAGSTLEEVGFDKRTTEAAKGSLYDSALSIVP
EEECCCHHHHHHHHCCCCEEEECCCCEEEECCCHHHHCCCCHHHHHHHCHHHHHHHHHCC
GLADCPVEHHWAGLRPGSPDGVPRIGAVPGVEGLWVNAGHYRNGLVLAPASTRLLADQLL
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCHHHHHHHHHH
QRTPVVDPAPYRLDT
HHCCCCCCCCCCCCC
>Mature Secondary Structure
MIDCGFRQPRTSVPVSEFLIVGGGVIGMMTALQLADAGRRVTLLERGECGREASWAGGGI
CCCCCCCCCCCCCCHHHHEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCC
VSPLYPWRYSAPISTLSRYSEGAYPELSLRLLEETGIDPEYRQRGLLYLRVDDEERALDW
CCCCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHCCEEEEEECCHHHHHHH
ARQEGKPLQRVGPETIYAKEPNAAPGVESALWMPTLGSIRNPRLCRALRARLQAMPNVAL
HHHCCCCHHHCCCCEEEECCCCCCCCCCHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHH
REHVDVEELVASAGRIQGVRTSAGHETAECVVVCGGAWASQLLASVDVALPVRPVRGQMI
HHCCCHHHHHHHHHHCCCEECCCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCCCCCCEE
LFKAPPGLVERVVLKDGRYVIPRGDGRVVAGSTLEEVGFDKRTTEAAKGSLYDSALSIVP
EEECCCHHHHHHHHCCCCEEEECCCCEEEECCCHHHHCCCCHHHHHHHCHHHHHHHHHCC
GLADCPVEHHWAGLRPGSPDGVPRIGAVPGVEGLWVNAGHYRNGLVLAPASTRLLADQLL
CCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEECCCHHHHHHHHHH
QRTPVVDPAPYRLDT
HHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {D-proline}} 0.13 {K3Fe(CN)6}} 11 {D-methionine}} 6 {D-phenylalanine}} 12 {D-alpha-aminobutyrate}} 14 {D-glutamine}} 0.63 {dichloroindophenol}} 0.01 {dichloroindophenol}} 0.15 {cytochrome} 19 {D-serine}} 29 {D-alanine}} 6.4

Substrates: D-Alanine; FAD; H2O [C]

Specific reaction: D-Alanine + FAD + H2O --> FADH2 + ammonia + Pyruvate [C]

General reaction: Oxidative deamination; Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8682785; 10984043; 8097014 [H]