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The map label for this gene is proB

Identifier: 92112610

GI number: 92112610

Start: 525380

End: 526516

Strand: Direct

Name: proB

Synonym: Csal_0477

Alternate gene names: 92112610

Gene position: 525380-526516 (Clockwise)

Preceding gene: 92112609

Following gene: 92112611

Centisome position: 14.21

GC content: 68.6

Gene sequence:

>1137_bases
GTGAGCGAATCGCAGTCGGCAAGCCGCGATGCCCTGCGTCGGGCGCGGCGTGTCGTGGTCAAGATCGGAAGTGCGTTGCT
GACCAACGATGGGCGCGGCCTCGATGCCGATGCCATCGGCGAATGGGTCGATCAGATCGCCGCCTTGCATGCGCGGGGCA
TCGAGGTCGTGCTGGTGTCGTCCGGTGCCGTGGCCGAGGGCATGGCGCGCCTGGGGTGGGTGACGCGTCCCTCGGCGGTG
CATGAATTGCAGGCGGCGGCGGCGGTAGGGCAATCCGGCCTGTCGCAGTGTTACGAGGAACATTTCGCGCGGCATGGCCT
GCGCAGTGCGCAGGTCCTGTTGACCCACGACGACCTTTCCAACCGCAAGCGATATCTCAATGCGCGTTCGGCGCTGCGCT
CGCTGGTGGCGCTGCGCGTGGTGCCGGTGATCAATGAAAACGACACCGTGGTCACCGACGAGATTCGCTTCGGCGACAAC
GATACGCTGGGCGCGCTGGTGGCCAATCTGCTCGAGGCGGATGCCCTCGTCCTGCTGACCGATCAGGAAGGCTTGTTCGA
CGCCGATCCTCGTCACGACCCCGAGGCGCGCCTGATTGCCGAGGGGCGTGCCGATGACCCGGCGCTGGCCGCGGTCGCCG
GTGACGGCGGTGCCCTGGGGCGTGGCGGGATGGCGACCAAGGTCCGTGCGGCTCGTCTGGCCGCTCGCTCCGGCGCGCTG
ACGGCGATCGCCAGCGGGCGTCAGCCGGAGGTGCTCACGCGGCTGATGCAAGGCGAGACGCTGGGCACGCTGTTGATGCC
CGATCACGCTCCCCTGGCGGCCCGCAAGCGGTGGCTGGCCGGGCAGCTTCAGGTGCGCGGCACCCTGGTGCTGGATGCCG
GCGCGGTCAAGGTGCTGCGCGAGCATGGCTCCAGCTTGCTGCCGGTGGGCGTGCGTCGTGTCGAGGGCGAGTTCAAGCGC
GGCGACATGCTGGTCTGCGTGGACGAGCAGGGCCAGCGCATCGCCAAGGGGCTGGTCAATTACGGCAGCGACGAAGCGCG
GCGCATCATGGGGCAGCCCAGCCGGCGCATCGAGGAGCTGCTGGGGTACATGGAGTCGCCGGAACTCATTCATCGCGACA
ACCTGGTCATCGTGTGA

Upstream 100 bases:

>100_bases
CGCAAGCCGGCCGAGGACGATGACGACGATTTCGACGAAGACGATTACGACGTCGAAGTGGAGTACGCGCCGTGATGCGG
CCGCAAGGAGACGTGAACCG

Downstream 100 bases:

>100_bases
GCGGCGGTCGGCGGGCATGTCTCGAAAGGCGCGACCTGTAGCCGTTGTCGCGGGGAGCATGCTAGAATGCCGCATCCTCT
CAGACATGGCCCGTTGATGG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSESQSASRDALRRARRVVVKIGSALLTNDGRGLDADAIGEWVDQIAALHARGIEVVLVSSGAVAEGMARLGWVTRPSAV
HELQAAAAVGQSGLSQCYEEHFARHGLRSAQVLLTHDDLSNRKRYLNARSALRSLVALRVVPVINENDTVVTDEIRFGDN
DTLGALVANLLEADALVLLTDQEGLFDADPRHDPEARLIAEGRADDPALAAVAGDGGALGRGGMATKVRAARLAARSGAL
TAIASGRQPEVLTRLMQGETLGTLLMPDHAPLAARKRWLAGQLQVRGTLVLDAGAVKVLREHGSSLLPVGVRRVEGEFKR
GDMLVCVDEQGQRIAKGLVNYGSDEARRIMGQPSRRIEELLGYMESPELIHRDNLVIV

Sequences:

>Translated_378_residues
MSESQSASRDALRRARRVVVKIGSALLTNDGRGLDADAIGEWVDQIAALHARGIEVVLVSSGAVAEGMARLGWVTRPSAV
HELQAAAAVGQSGLSQCYEEHFARHGLRSAQVLLTHDDLSNRKRYLNARSALRSLVALRVVPVINENDTVVTDEIRFGDN
DTLGALVANLLEADALVLLTDQEGLFDADPRHDPEARLIAEGRADDPALAAVAGDGGALGRGGMATKVRAARLAARSGAL
TAIASGRQPEVLTRLMQGETLGTLLMPDHAPLAARKRWLAGQLQVRGTLVLDAGAVKVLREHGSSLLPVGVRRVEGEFKR
GDMLVCVDEQGQRIAKGLVNYGSDEARRIMGQPSRRIEELLGYMESPELIHRDNLVIV
>Mature_377_residues
SESQSASRDALRRARRVVVKIGSALLTNDGRGLDADAIGEWVDQIAALHARGIEVVLVSSGAVAEGMARLGWVTRPSAVH
ELQAAAAVGQSGLSQCYEEHFARHGLRSAQVLLTHDDLSNRKRYLNARSALRSLVALRVVPVINENDTVVTDEIRFGDND
TLGALVANLLEADALVLLTDQEGLFDADPRHDPEARLIAEGRADDPALAAVAGDGGALGRGGMATKVRAARLAARSGALT
AIASGRQPEVLTRLMQGETLGTLLMPDHAPLAARKRWLAGQLQVRGTLVLDAGAVKVLREHGSSLLPVGVRRVEGEFKRG
DMLVCVDEQGQRIAKGLVNYGSDEARRIMGQPSRRIEELLGYMESPELIHRDNLVIV

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=294, Percent_Identity=34.6938775510204, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI62912457, Length=292, Percent_Identity=34.9315068493151, Blast_Score=142, Evalue=7e-34,
Organism=Escherichia coli, GI1786437, Length=377, Percent_Identity=45.0928381962865, Blast_Score=301, Evalue=5e-83,
Organism=Caenorhabditis elegans, GI17569969, Length=274, Percent_Identity=32.1167883211679, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17569967, Length=274, Percent_Identity=32.1167883211679, Blast_Score=132, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6321822, Length=420, Percent_Identity=32.1428571428571, Blast_Score=195, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6320506, Length=419, Percent_Identity=32.2195704057279, Blast_Score=191, Evalue=2e-49,
Organism=Drosophila melanogaster, GI21357643, Length=289, Percent_Identity=35.6401384083045, Blast_Score=148, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_CHRSD (Q1R0B9)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_572538.1
- ProteinModelPortal:   Q1R0B9
- SMR:   Q1R0B9
- STRING:   Q1R0B9
- GeneID:   4026501
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_0477
- NMPDR:   fig|290398.4.peg.15
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   CSAL290398:CSAL_0477-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40527; Mature: 40396

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESQSASRDALRRARRVVVKIGSALLTNDGRGLDADAIGEWVDQIAALHARGIEVVLVS
CCCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEE
SGAVAEGMARLGWVTRPSAVHELQAAAAVGQSGLSQCYEEHFARHGLRSAQVLLTHDDLS
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHH
NRKRYLNARSALRSLVALRVVPVINENDTVVTDEIRFGDNDTLGALVANLLEADALVLLT
HHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEE
DQEGLFDADPRHDPEARLIAEGRADDPALAAVAGDGGALGRGGMATKVRAARLAARSGAL
CCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCE
TAIASGRQPEVLTRLMQGETLGTLLMPDHAPLAARKRWLAGQLQVRGTLVLDAGAVKVLR
EEECCCCCHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCEEEEEEEEEECHHHHHHHH
EHGSSLLPVGVRRVEGEFKRGDMLVCVDEQGQRIAKGLVNYGSDEARRIMGQPSRRIEEL
HCCCCCCCHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHH
LGYMESPELIHRDNLVIV
HHHCCCCCEEECCCEEEC
>Mature Secondary Structure 
SESQSASRDALRRARRVVVKIGSALLTNDGRGLDADAIGEWVDQIAALHARGIEVVLVS
CCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEEEE
SGAVAEGMARLGWVTRPSAVHELQAAAAVGQSGLSQCYEEHFARHGLRSAQVLLTHDDLS
CCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHH
NRKRYLNARSALRSLVALRVVPVINENDTVVTDEIRFGDNDTLGALVANLLEADALVLLT
HHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCCCCHHHHHHHHHCCCEEEEEE
DQEGLFDADPRHDPEARLIAEGRADDPALAAVAGDGGALGRGGMATKVRAARLAARSGAL
CCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCE
TAIASGRQPEVLTRLMQGETLGTLLMPDHAPLAARKRWLAGQLQVRGTLVLDAGAVKVLR
EEECCCCCHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHCCEEEEEEEEEECHHHHHHHH
EHGSSLLPVGVRRVEGEFKRGDMLVCVDEQGQRIAKGLVNYGSDEARRIMGQPSRRIEEL
HCCCCCCCHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHHHCCCHHHHHHH
LGYMESPELIHRDNLVIV
HHHCCCCCEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA