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The map label for this gene is hmp [H]

Identifier: 92112585

GI number: 92112585

Start: 500065

End: 501246

Strand: Direct

Name: hmp [H]

Synonym: Csal_0452

Alternate gene names: 92112585

Gene position: 500065-501246 (Clockwise)

Preceding gene: 92112583

Following gene: 92112587

Centisome position: 13.53

GC content: 67.85

Gene sequence:

>1182_bases
ATGCTTACATCCGCCCAGGAATCCGTCATTGCTGCCACCACGCCCGTCGTCGCCGAACACATCGAGGCCATCGCGCAGCG
CTTCTATCCGCTGATGTTCGCGCGCTATCCGGAAGTGAAGACGCTGTTCAACGCCACTCATCAGCATACGGGCGGACAGC
CCCGGGCCCTGGCGGGCGCCGTCGTCGGCTATGTCCAGCTGCGCCACACGCCGCAGCGCCTCGACGAAGTGTTGGGCATC
ATCGTCGACAAGCATGTCTCGCTGGGCATACGGCCCGAGCACTATCCGATCGTCGGCGAATGCCTGATGGCGGCGATCGG
CGAGGTGTTGGGCGATGCGGTGACGGACGAGGTCGCCGATGCCTGGGGGGCGCTATACGAGGAACTGGCGGGCTTGCTGA
TCGAGAAGGAAGCCCGCCGCTATCGCGACTTCGCCGCGCAGCCGGGGGGCTGGGAAGGTACGCGGACCTTCCGTATCGCC
GCCAAGCGCCCCGAAAGCGCCGTGATCACCTCCTTCGTGCTGGCGCCGGTGGACGGCGGCCCCGTCGCCGCGCATCGCCC
TGGGCAATACATCGGCTTCAAGCTGACGATCGACGGCGAGATCGTGCATCGCCACTATAGCCTGTCAGCCTCGCCCGACG
GGAAGACCTACCGCGTATCCATCAAGCGCGAGGAGGGGGGCAAGGTCAGCCGCCATTTCCATGATCGCCTGGCCGAGGGC
GATACGCTGGAGCTGTTGCCGCCGGCCGGCCATCTGACGCTGGACGAGGGCACATCGCCGGTGATGTTGATCAGCGCGGG
CGTCGGCCAGACCCCGATGGTGCCGCTGGCACGGCAGGCGCTCGCCGCCGGGCGCCGGGTGGCCTATCTGCACGCCGCGC
GCGATGGGGCGGTGCATGCCTTCGCGGATGAACTGGCGCGACTCGAGGCGGATTACCCGGCGTTGCTGGAGGTGATCACC
GTATATTCGCAGCCACGGGCGGAAGATGCACCGACGCACGGCGGTCATGTGGACGCCAACCTGCTGTCGCGCTATCTGCC
GCGCGACGCCCAGTGCTACCTGGTCGGGCCGGCGGGGTTCATGCGCAACATGGAGGCTGCGCTGAGCGCGCTCGGTGTCG
CGGCCAGCCGGCGGCATCATGAGTTCTTCGGGCCCGCCGAGCCGTTGGACAGCGCCGCCTGA

Upstream 100 bases:

>100_bases
GTGGCACGATGTCGCGGTGGCGGCGCGCCGGGCCAGCTTGGCACTCTGCTTGCGATAGAGGGAATGCATCTTGTGCAGAA
CCTTTATCCAAGGAGTCGTC

Downstream 100 bases:

>100_bases
GAAGGCCGCGCTCCTGAACGCCACGCCAGCCGGCGTGGCGTTTTGCATGGTGGCGAGTGTCTCAGGGCGCGGGACGATAC
GCGCACGGAGGCATGCCACG

Product: oxidoreductase FAD-binding protein

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MLTSAQESVIAATTPVVAEHIEAIAQRFYPLMFARYPEVKTLFNATHQHTGGQPRALAGAVVGYVQLRHTPQRLDEVLGI
IVDKHVSLGIRPEHYPIVGECLMAAIGEVLGDAVTDEVADAWGALYEELAGLLIEKEARRYRDFAAQPGGWEGTRTFRIA
AKRPESAVITSFVLAPVDGGPVAAHRPGQYIGFKLTIDGEIVHRHYSLSASPDGKTYRVSIKREEGGKVSRHFHDRLAEG
DTLELLPPAGHLTLDEGTSPVMLISAGVGQTPMVPLARQALAAGRRVAYLHAARDGAVHAFADELARLEADYPALLEVIT
VYSQPRAEDAPTHGGHVDANLLSRYLPRDAQCYLVGPAGFMRNMEAALSALGVAASRRHHEFFGPAEPLDSAA

Sequences:

>Translated_393_residues
MLTSAQESVIAATTPVVAEHIEAIAQRFYPLMFARYPEVKTLFNATHQHTGGQPRALAGAVVGYVQLRHTPQRLDEVLGI
IVDKHVSLGIRPEHYPIVGECLMAAIGEVLGDAVTDEVADAWGALYEELAGLLIEKEARRYRDFAAQPGGWEGTRTFRIA
AKRPESAVITSFVLAPVDGGPVAAHRPGQYIGFKLTIDGEIVHRHYSLSASPDGKTYRVSIKREEGGKVSRHFHDRLAEG
DTLELLPPAGHLTLDEGTSPVMLISAGVGQTPMVPLARQALAAGRRVAYLHAARDGAVHAFADELARLEADYPALLEVIT
VYSQPRAEDAPTHGGHVDANLLSRYLPRDAQCYLVGPAGFMRNMEAALSALGVAASRRHHEFFGPAEPLDSAA
>Mature_393_residues
MLTSAQESVIAATTPVVAEHIEAIAQRFYPLMFARYPEVKTLFNATHQHTGGQPRALAGAVVGYVQLRHTPQRLDEVLGI
IVDKHVSLGIRPEHYPIVGECLMAAIGEVLGDAVTDEVADAWGALYEELAGLLIEKEARRYRDFAAQPGGWEGTRTFRIA
AKRPESAVITSFVLAPVDGGPVAAHRPGQYIGFKLTIDGEIVHRHYSLSASPDGKTYRVSIKREEGGKVSRHFHDRLAEG
DTLELLPPAGHLTLDEGTSPVMLISAGVGQTPMVPLARQALAAGRRVAYLHAARDGAVHAFADELARLEADYPALLEVIT
VYSQPRAEDAPTHGGHVDANLLSRYLPRDAQCYLVGPAGFMRNMEAALSALGVAASRRHHEFFGPAEPLDSAA

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=402, Percent_Identity=42.7860696517413, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1787658, Length=211, Percent_Identity=30.3317535545024, Blast_Score=89, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321673, Length=410, Percent_Identity=30.2439024390244, Blast_Score=166, Evalue=4e-42,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 42446; Mature: 42446

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTSAQESVIAATTPVVAEHIEAIAQRFYPLMFARYPEVKTLFNATHQHTGGQPRALAGA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHH
VVGYVQLRHTPQRLDEVLGIIVDKHVSLGIRPEHYPIVGECLMAAIGEVLGDAVTDEVAD
HHHHHHHCCCHHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AWGALYEELAGLLIEKEARRYRDFAAQPGGWEGTRTFRIAAKRPESAVITSFVLAPVDGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHEEECCCCC
PVAAHRPGQYIGFKLTIDGEIVHRHYSLSASPDGKTYRVSIKREEGGKVSRHFHDRLAEG
CCCCCCCCCEEEEEEEECCEEEEEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHCCC
DTLELLPPAGHLTLDEGTSPVMLISAGVGQTPMVPLARQALAAGRRVAYLHAARDGAVHA
CEEEEECCCCCEEECCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHH
FADELARLEADYPALLEVITVYSQPRAEDAPTHGGHVDANLLSRYLPRDAQCYLVGPAGF
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCHHH
MRNMEAALSALGVAASRRHHEFFGPAEPLDSAA
HHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCC
>Mature Secondary Structure
MLTSAQESVIAATTPVVAEHIEAIAQRFYPLMFARYPEVKTLFNATHQHTGGQPRALAGA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHH
VVGYVQLRHTPQRLDEVLGIIVDKHVSLGIRPEHYPIVGECLMAAIGEVLGDAVTDEVAD
HHHHHHHCCCHHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AWGALYEELAGLLIEKEARRYRDFAAQPGGWEGTRTFRIAAKRPESAVITSFVLAPVDGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCHHHHHHHEEECCCCC
PVAAHRPGQYIGFKLTIDGEIVHRHYSLSASPDGKTYRVSIKREEGGKVSRHFHDRLAEG
CCCCCCCCCEEEEEEEECCEEEEEEEEECCCCCCCEEEEEEEECCCCCHHHHHHHHHCCC
DTLELLPPAGHLTLDEGTSPVMLISAGVGQTPMVPLARQALAAGRRVAYLHAARDGAVHA
CEEEEECCCCCEEECCCCCCEEEEECCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCHHH
FADELARLEADYPALLEVITVYSQPRAEDAPTHGGHVDANLLSRYLPRDAQCYLVGPAGF
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEECCHHH
MRNMEAALSALGVAASRRHHEFFGPAEPLDSAA
HHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]