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The map label for this gene is tuf

Identifier: 92112552

GI number: 92112552

Start: 478004

End: 479197

Strand: Direct

Name: tuf

Synonym: Csal_0419

Alternate gene names: 92112552

Gene position: 478004-479197 (Clockwise)

Preceding gene: 92112551

Following gene: 92112553

Centisome position: 12.93

GC content: 62.06

Gene sequence:

>1194_bases
GTGGCTAAGGAAAAGTTTGAACGTAATAAAGCGCACATCAACGTTGGCACCATCGGTCACGTCGACCATGGCAAGACCAC
GCTGACTGCGGCACTGACTCGCGTTTCCGCGGAGGTCTTCGGCGGCGACTGGCGCGAATTCGACACCATCGACAACGCAC
CGGAAGAGCGTGAGCGCGGTATCACCATCGCCACCGCTCACGTCGAGTACCAGTCCGAAGAGCGCCACTACGCGCACGTC
GACTGCCCGGGGCACGCCGACTACGTCAAGAACATGATCACCGGTGCCGCTCAGATGGATGGCGCCATTCTGGTCTGTTC
CGCAGCTGACGGCCCCATGCCGCAGACTCGCGAGCACATCCTGCTGTCTCGCCAGGTCGGCGTTCCGACCATCGTCGTGT
TCCTGAACAAGGCCGACATGGTCGACGACGAAGAGCTCCTCGAGCTGGTCGAAATGGAAGTTCGTGAACTCCTCAACGAG
TACGACTTCCCGGGCGACGACTGCCCGATCATCACCGGTTCCGCGCTGATGGCGCTGGAAGGCAAGGACGACAACGGCAT
GGGTACCACTGCCGTCGCCAACCTGATCAAGGCCCTGGATGCCTACATCCCGGAGCCGGAGCGTGCCATCGACCAGCCGT
TCCTGATGCCGATCGAAGACGTGTTCTCGATCTCCGGCCGCGGTACCGTCGTGACCGGTCGTGTCGAGCGCGGTATCGTC
AAGTCTGGCGAAGAAGTCGAGATCGTGGGTCTCAAGGACACTGCCAAGACGACCGTTACCGGTGTCGAGATGTTCCGCAA
GCTGCTCGACGAAGGTCGTGCCGGCGAGAACATCGGCGCGCTGCTGCGCGGCACCAAGCGTGACGACGTCGAGCGTGGCC
AGGTGCTGGCCAAGCCGGGCACCATCACGCCGCACACTGTCTTCGAAGCCGAAGTCTATGTGCTGTCCAAGGAAGAAGGC
GGTCGTCACACGCCGTTCTTCAAGGGCTATCGTCCGCAGTTCTACTTCCGGACCACTGACGTGACCGGTACCTGCGAGCT
GCCGGAAGGCGTCGAAATGGTCATGCCGGGCGACAACGTCAAGATGACCGTGACCCTGATCGCGCCGATCGCCATGGAAG
ACGGCCTGCGCTTCGCTGTTCGCGAAGGCGGTCGTACCGTAGGCGCCGGTGTCGTCGCCAAGATCGTCCAGTAA

Upstream 100 bases:

>100_bases
CTATGGAGTTTGCGGATTACGAAGAGGCGCCCTCCAGCGTCGTTGAAGCCGTCATCAACCAGAATGGTTAACCGTTAGCT
AACGTAAAGAGGTTATAGCA

Downstream 100 bases:

>100_bases
GGGTTTCGCCCTTCGATGATCGAAGGGGGCTCCTTTGAGGAGCCCCCTTTCTGTGCATGTTTGACAGTCACTGTCAGCCT
GCCTATAATGCGCATCCTTT

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MAKEKFERNKAHINVGTIGHVDHGKTTLTAALTRVSAEVFGGDWREFDTIDNAPEERERGITIATAHVEYQSEERHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPTIVVFLNKADMVDDEELLELVEMEVRELLNE
YDFPGDDCPIITGSALMALEGKDDNGMGTTAVANLIKALDAYIPEPERAIDQPFLMPIEDVFSISGRGTVVTGRVERGIV
KSGEEVEIVGLKDTAKTTVTGVEMFRKLLDEGRAGENIGALLRGTKRDDVERGQVLAKPGTITPHTVFEAEVYVLSKEEG
GRHTPFFKGYRPQFYFRTTDVTGTCELPEGVEMVMPGDNVKMTVTLIAPIAMEDGLRFAVREGGRTVGAGVVAKIVQ

Sequences:

>Translated_397_residues
MAKEKFERNKAHINVGTIGHVDHGKTTLTAALTRVSAEVFGGDWREFDTIDNAPEERERGITIATAHVEYQSEERHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPTIVVFLNKADMVDDEELLELVEMEVRELLNE
YDFPGDDCPIITGSALMALEGKDDNGMGTTAVANLIKALDAYIPEPERAIDQPFLMPIEDVFSISGRGTVVTGRVERGIV
KSGEEVEIVGLKDTAKTTVTGVEMFRKLLDEGRAGENIGALLRGTKRDDVERGQVLAKPGTITPHTVFEAEVYVLSKEEG
GRHTPFFKGYRPQFYFRTTDVTGTCELPEGVEMVMPGDNVKMTVTLIAPIAMEDGLRFAVREGGRTVGAGVVAKIVQ
>Mature_396_residues
AKEKFERNKAHINVGTIGHVDHGKTTLTAALTRVSAEVFGGDWREFDTIDNAPEERERGITIATAHVEYQSEERHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPTIVVFLNKADMVDDEELLELVEMEVRELLNEY
DFPGDDCPIITGSALMALEGKDDNGMGTTAVANLIKALDAYIPEPERAIDQPFLMPIEDVFSISGRGTVVTGRVERGIVK
SGEEVEIVGLKDTAKTTVTGVEMFRKLLDEGRAGENIGALLRGTKRDDVERGQVLAKPGTITPHTVFEAEVYVLSKEEGG
RHTPFFKGYRPQFYFRTTDVTGTCELPEGVEMVMPGDNVKMTVTLIAPIAMEDGLRFAVREGGRTVGAGVVAKIVQ

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily

Homologues:

Organism=Homo sapiens, GI34147630, Length=391, Percent_Identity=56.2659846547315, Blast_Score=471, Evalue=1e-133,
Organism=Homo sapiens, GI4503471, Length=456, Percent_Identity=29.6052631578947, Blast_Score=163, Evalue=3e-40,
Organism=Homo sapiens, GI4503475, Length=451, Percent_Identity=28.6031042128603, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI223555963, Length=443, Percent_Identity=27.0880361173815, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI5729864, Length=443, Percent_Identity=27.0880361173815, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI194018522, Length=335, Percent_Identity=28.6567164179104, Blast_Score=123, Evalue=4e-28,
Organism=Homo sapiens, GI194018520, Length=335, Percent_Identity=28.6567164179104, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI194097354, Length=335, Percent_Identity=28.6567164179104, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI46094014, Length=337, Percent_Identity=29.080118694362, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI54607086, Length=352, Percent_Identity=28.6931818181818, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI157426893, Length=301, Percent_Identity=25.2491694352159, Blast_Score=78, Evalue=2e-14,
Organism=Escherichia coli, GI1790412, Length=397, Percent_Identity=79.5969773299748, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI1789737, Length=396, Percent_Identity=79.7979797979798, Blast_Score=621, Evalue=1e-179,
Organism=Escherichia coli, GI2367247, Length=385, Percent_Identity=30.1298701298701, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI48994988, Length=238, Percent_Identity=33.1932773109244, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789108, Length=319, Percent_Identity=29.4670846394984, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1788922, Length=147, Percent_Identity=31.9727891156463, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=402, Percent_Identity=50.7462686567164, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25141371, Length=388, Percent_Identity=45.1030927835052, Blast_Score=341, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI17552884, Length=451, Percent_Identity=29.490022172949, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=451, Percent_Identity=29.490022172949, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=436, Percent_Identity=29.8165137614679, Blast_Score=140, Evalue=9e-34,
Organism=Caenorhabditis elegans, GI32566629, Length=452, Percent_Identity=26.5486725663717, Blast_Score=124, Evalue=6e-29,
Organism=Caenorhabditis elegans, GI115532067, Length=449, Percent_Identity=26.5033407572383, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI115532065, Length=449, Percent_Identity=26.7260579064588, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17506081, Length=274, Percent_Identity=30.2919708029197, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=35.5704697986577, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=234, Percent_Identity=27.3504273504274, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17509919, Length=456, Percent_Identity=25.4385964912281, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=397, Percent_Identity=63.4760705289673, Blast_Score=518, Evalue=1e-148,
Organism=Saccharomyces cerevisiae, GI6325337, Length=440, Percent_Identity=29.0909090909091, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=440, Percent_Identity=29.0909090909091, Blast_Score=159, Evalue=6e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=455, Percent_Identity=23.7362637362637, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320377, Length=339, Percent_Identity=28.023598820059, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320863, Length=286, Percent_Identity=26.9230769230769, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=239, Percent_Identity=28.4518828451883, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281363316, Length=390, Percent_Identity=56.6666666666667, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI17864358, Length=390, Percent_Identity=56.6666666666667, Blast_Score=464, Evalue=1e-131,
Organism=Drosophila melanogaster, GI19921738, Length=387, Percent_Identity=47.8036175710594, Blast_Score=392, Evalue=1e-109,
Organism=Drosophila melanogaster, GI45553807, Length=449, Percent_Identity=28.7305122494432, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI45553816, Length=449, Percent_Identity=28.7305122494432, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24651721, Length=449, Percent_Identity=28.7305122494432, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI17864154, Length=449, Percent_Identity=28.7305122494432, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=28.9532293986637, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=28.9532293986637, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI19922690, Length=306, Percent_Identity=31.3725490196078, Blast_Score=132, Evalue=6e-31,
Organism=Drosophila melanogaster, GI45550900, Length=453, Percent_Identity=26.9315673289183, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI17137380, Length=344, Percent_Identity=30.2325581395349, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24647046, Length=373, Percent_Identity=26.8096514745308, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24647048, Length=373, Percent_Identity=26.8096514745308, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): EFTU_CHRSD (Q1R0H7)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_572480.1
- ProteinModelPortal:   Q1R0H7
- SMR:   Q1R0H7
- STRING:   Q1R0H7
- GeneID:   4025883
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_0419
- NMPDR:   fig|290398.4.peg.639
- eggNOG:   COG0050
- HOGENOM:   HBG307581
- OMA:   IAPIACE
- ProtClustDB:   PRK00049
- BioCyc:   CSAL290398:CSAL_0419-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00118_B
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000
- PANTHER:   PTHR23115:SF31
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00485
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: 3.6.5.3

Molecular weight: Translated: 43346; Mature: 43215

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERNKAHINVGTIGHVDHGKTTLTAALTRVSAEVFGGDWREFDTIDNAPEERERG
CCCHHHCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHCC
ITIATAHVEYQSEERHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPTIVVFLNKADMVDDEELLELVEMEVRELLNEYDFPGDDCPIITGSALMALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GKDDNGMGTTAVANLIKALDAYIPEPERAIDQPFLMPIEDVFSISGRGTVVTGRVERGIV
CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHCCCCCCEEEECHHHHHHH
KSGEEVEIVGLKDTAKTTVTGVEMFRKLLDEGRAGENIGALLRGTKRDDVERGQVLAKPG
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEECCC
TITPHTVFEAEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGTCELPEGVEMVMPGDNV
CCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCEEEECCCCC
KMTVTLIAPIAMEDGLRFAVREGGRTVGAGVVAKIVQ
EEEEEEEEEEHHCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFERNKAHINVGTIGHVDHGKTTLTAALTRVSAEVFGGDWREFDTIDNAPEERERG
CCHHHCCCCCEEEECEEECCCCCCHHHHHHHHHHHHHHHCCCCHHCCCCCCCCHHHHCC
ITIATAHVEYQSEERHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPTIVVFLNKADMVDDEELLELVEMEVRELLNEYDFPGDDCPIITGSALMALE
EEECCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GKDDNGMGTTAVANLIKALDAYIPEPERAIDQPFLMPIEDVFSISGRGTVVTGRVERGIV
CCCCCCCCHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHHCCCCCCEEEECHHHHHHH
KSGEEVEIVGLKDTAKTTVTGVEMFRKLLDEGRAGENIGALLRGTKRDDVERGQVLAKPG
CCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCEEEECCC
TITPHTVFEAEVYVLSKEEGGRHTPFFKGYRPQFYFRTTDVTGTCELPEGVEMVMPGDNV
CCCCCEEEEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCEEEECCCCC
KMTVTLIAPIAMEDGLRFAVREGGRTVGAGVVAKIVQ
EEEEEEEEEEHHCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA