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The map label for this gene is metK

Identifier: 92112518

GI number: 92112518

Start: 431204

End: 432424

Strand: Direct

Name: metK

Synonym: Csal_0385

Alternate gene names: 92112518

Gene position: 431204-432424 (Clockwise)

Preceding gene: 92112516

Following gene: 92112519

Centisome position: 11.66

GC content: 63.39

Gene sequence:

>1221_bases
ATGAACGAATATTCCCTGTTTACTTCCGAATCCGTCTCCGAGGGACACCCGGACAAGATCGCCGATCAGATTTCCGATGC
GGTGCTGGATGCCATCATCGCCCGCGACAAGCAGGCTCGCGTGGCCTGCGAAACCATGGTCAAGACCGGTGTCGCCATCG
TGGCCGGCGAAATCACGACCTCCGCCTGGGTCGACCTGGAAGATCTGGTTCGTCGCGTCATCAACGATATCGGCTATACC
TCTTCGGACGTGGGCTTCGATGGCAACACCTGCGGCGTGCTCAACCTGATCGGCAAGCAGAGCGTGGACATCGCCCAGGG
GGTCGATCGTTCCAAACCCGAGGACCAGGGGGCCGGCGACCAGGGCTTGATGTTCGGTTATGCCACCAACGAAACCGATT
CCTACATGCCGGCGCCCATTCATTACTCGCACCGGCTGGTGGAGCGTCAGGCCGAACTGCGCAAGCACGGGCTGCTGCCG
TGGCTGCGTCCGGACGCCAAGAGTCAGTTGACCTTCCGCTACGACGCGCAGGGCAAGCCCTGTGCCGTCGACGCCGTGGT
GCTCTCCACCCAGCACGATCCGGACATCGATCAGGACGAGCTGCGCGAGATGGTCAAGCGCGAGATCATCGAGGAAGTGA
TTCCCGCCGAATGGCTGACCGAGACCACCAAGTATCACATCAACCCGACCGGCAACTTCGTCATCGGCGGGCCGGTGGGC
GACTGCGGACTGACCGGCCGCAAGATCATCGTCGATACCTACGGCGGCATGGCGCGTCATGGCGGTGGCGCGTTCTCCGG
CAAGGATCCTTCAAAGGTCGACCGCAGCGCCGCCTACGCTGGCCGCTACGTGGCCAAGAACGTCGTCGCGGCGGGCCTGG
CCGAGCGCTGCGAGATCCAGGTCTCTTATGCCATCGGGGTGGCCGAGCCGACCTCCGTGTCGGTGGATACCTTCGGTACC
GGCAAGATCAGCGATGCCAAGATCGTCGAGCTGGTACGCGAGCATTTCGATCTGCGTCCCAACGCGATCACGCGCATGCT
CGACCTGCTGCATCCCATGTATCAGCTGACGGCCGCCTATGGTCACTTCGGCCGCGAGCCCTTCGAGACCAGCTACACCT
GGACCGACGTGGGCGGCAAGCAGCATGTCGAGACCTTCACGGCCTTCCCCTGGGAGAAGACCGACCGCGCCGACGCACTG
CGTCAGGCGGCAGGCCTCTGA

Upstream 100 bases:

>100_bases
GTGCCCGCCATTCCGGGTGGCCTGCGGCGTAGTTTCATCTCCTTTCGATGGCGGTCGTGTTCCGGCTCGCCAGCACGTGA
TAGAGAGAGTCGAGGACGCC

Downstream 100 bases:

>100_bases
GGACGCCTCGACACGAGCATCGAGAATCGAGCGGCATGCAGCGGCGTGTCGCGCCATCATCCGCAAAAGCCCCGGTTCGC
CGGGGCTTTTGCGTAGGACG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MNEYSLFTSESVSEGHPDKIADQISDAVLDAIIARDKQARVACETMVKTGVAIVAGEITTSAWVDLEDLVRRVINDIGYT
SSDVGFDGNTCGVLNLIGKQSVDIAQGVDRSKPEDQGAGDQGLMFGYATNETDSYMPAPIHYSHRLVERQAELRKHGLLP
WLRPDAKSQLTFRYDAQGKPCAVDAVVLSTQHDPDIDQDELREMVKREIIEEVIPAEWLTETTKYHINPTGNFVIGGPVG
DCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNVVAAGLAERCEIQVSYAIGVAEPTSVSVDTFGT
GKISDAKIVELVREHFDLRPNAITRMLDLLHPMYQLTAAYGHFGREPFETSYTWTDVGGKQHVETFTAFPWEKTDRADAL
RQAAGL

Sequences:

>Translated_406_residues
MNEYSLFTSESVSEGHPDKIADQISDAVLDAIIARDKQARVACETMVKTGVAIVAGEITTSAWVDLEDLVRRVINDIGYT
SSDVGFDGNTCGVLNLIGKQSVDIAQGVDRSKPEDQGAGDQGLMFGYATNETDSYMPAPIHYSHRLVERQAELRKHGLLP
WLRPDAKSQLTFRYDAQGKPCAVDAVVLSTQHDPDIDQDELREMVKREIIEEVIPAEWLTETTKYHINPTGNFVIGGPVG
DCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNVVAAGLAERCEIQVSYAIGVAEPTSVSVDTFGT
GKISDAKIVELVREHFDLRPNAITRMLDLLHPMYQLTAAYGHFGREPFETSYTWTDVGGKQHVETFTAFPWEKTDRADAL
RQAAGL
>Mature_406_residues
MNEYSLFTSESVSEGHPDKIADQISDAVLDAIIARDKQARVACETMVKTGVAIVAGEITTSAWVDLEDLVRRVINDIGYT
SSDVGFDGNTCGVLNLIGKQSVDIAQGVDRSKPEDQGAGDQGLMFGYATNETDSYMPAPIHYSHRLVERQAELRKHGLLP
WLRPDAKSQLTFRYDAQGKPCAVDAVVLSTQHDPDIDQDELREMVKREIIEEVIPAEWLTETTKYHINPTGNFVIGGPVG
DCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNVVAAGLAERCEIQVSYAIGVAEPTSVSVDTFGT
GKISDAKIVELVREHFDLRPNAITRMLDLLHPMYQLTAAYGHFGREPFETSYTWTDVGGKQHVETFTAFPWEKTDRADAL
RQAAGL

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=369, Percent_Identity=57.9945799457995, Blast_Score=406, Evalue=1e-113,
Organism=Homo sapiens, GI4557737, Length=368, Percent_Identity=57.3369565217391, Blast_Score=402, Evalue=1e-112,
Organism=Escherichia coli, GI1789311, Length=401, Percent_Identity=70.573566084788, Blast_Score=582, Evalue=1e-167,
Organism=Caenorhabditis elegans, GI17538498, Length=367, Percent_Identity=57.4931880108992, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17551082, Length=372, Percent_Identity=55.6451612903226, Blast_Score=403, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17538494, Length=367, Percent_Identity=56.9482288828338, Blast_Score=402, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25145633, Length=367, Percent_Identity=56.6757493188011, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI32565905, Length=367, Percent_Identity=51.2261580381471, Blast_Score=343, Evalue=8e-95,
Organism=Caenorhabditis elegans, GI32565911, Length=320, Percent_Identity=55, Blast_Score=333, Evalue=7e-92,
Organism=Caenorhabditis elegans, GI25145635, Length=320, Percent_Identity=54.0625, Blast_Score=327, Evalue=8e-90,
Organism=Caenorhabditis elegans, GI32565903, Length=295, Percent_Identity=54.2372881355932, Blast_Score=304, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=62.6506024096386, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320710, Length=373, Percent_Identity=55.7640750670241, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323209, Length=373, Percent_Identity=56.5683646112601, Blast_Score=392, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45552159, Length=367, Percent_Identity=53.4059945504087, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580529, Length=367, Percent_Identity=53.4059945504087, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580537, Length=367, Percent_Identity=53.4059945504087, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580533, Length=367, Percent_Identity=53.4059945504087, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580535, Length=367, Percent_Identity=53.4059945504087, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580531, Length=367, Percent_Identity=53.4059945504087, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24580527, Length=367, Percent_Identity=53.4059945504087, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580525, Length=367, Percent_Identity=53.4059945504087, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580541, Length=369, Percent_Identity=50.9485094850949, Blast_Score=344, Evalue=7e-95,
Organism=Drosophila melanogaster, GI24580539, Length=117, Percent_Identity=54.7008547008547, Blast_Score=121, Evalue=8e-28,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_CHRSD (Q1R0L1)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_572446.1
- ProteinModelPortal:   Q1R0L1
- SMR:   Q1R0L1
- STRING:   Q1R0L1
- GeneID:   4026564
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_0385
- NMPDR:   fig|290398.4.peg.463
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   VEYYNDN
- ProtClustDB:   PRK05250
- BioCyc:   CSAL290398:CSAL_0385-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 44386; Mature: 44386

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEYSLFTSESVSEGHPDKIADQISDAVLDAIIARDKQARVACETMVKTGVAIVAGEITT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCH
SAWVDLEDLVRRVINDIGYTSSDVGFDGNTCGVLNLIGKQSVDIAQGVDRSKPEDQGAGD
HHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHCCCCCCCCCCCCCC
QGLMFGYATNETDSYMPAPIHYSHRLVERQAELRKHGLLPWLRPDAKSQLTFRYDAQGKP
CCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCC
CAVDAVVLSTQHDPDIDQDELREMVKREIIEEVIPAEWLTETTKYHINPTGNFVIGGPVG
EEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEECCCCC
DCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNVVAAGLAERCEIQ
CCCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
VSYAIGVAEPTSVSVDTFGTGKISDAKIVELVREHFDLRPNAITRMLDLLHPMYQLTAAY
EEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GHFGREPFETSYTWTDVGGKQHVETFTAFPWEKTDRADALRQAAGL
HHCCCCCCCCCCEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNEYSLFTSESVSEGHPDKIADQISDAVLDAIIARDKQARVACETMVKTGVAIVAGEITT
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEECCCH
SAWVDLEDLVRRVINDIGYTSSDVGFDGNTCGVLNLIGKQSVDIAQGVDRSKPEDQGAGD
HHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCCCHHHHCCCCCCCCCCCCCC
QGLMFGYATNETDSYMPAPIHYSHRLVERQAELRKHGLLPWLRPDAKSQLTFRYDAQGKP
CCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCCCC
CAVDAVVLSTQHDPDIDQDELREMVKREIIEEVIPAEWLTETTKYHINPTGNFVIGGPVG
EEEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEECCCCCEEECCCCC
DCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYAGRYVAKNVVAAGLAERCEIQ
CCCCCCCEEEEECCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEE
VSYAIGVAEPTSVSVDTFGTGKISDAKIVELVREHFDLRPNAITRMLDLLHPMYQLTAAY
EEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GHFGREPFETSYTWTDVGGKQHVETFTAFPWEKTDRADALRQAAGL
HHCCCCCCCCCCEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA