Click here to switch to the map view.

The map label for this gene is ywlF [H]

Identifier: 92112490

GI number: 92112490

Start: 400279

End: 400779

Strand: Direct

Name: ywlF [H]

Synonym: Csal_0357

Alternate gene names: 92112490

Gene position: 400279-400779 (Clockwise)

Preceding gene: 92112484

Following gene: 92112491

Centisome position: 10.83

GC content: 66.47

Gene sequence:

>501_bases
ATGTCAGATTCACGCACGGCCAGTCATACGGCCCCGATGCGCGTTGCCCTGGGGGGCGACGAGGCCGCCTACGAGCTCAA
GGCGTTGCTGATCGAGCACGTTCGAGCGCTGGGGCATGAGGCGGTGGACTTCGGCACCTATGATGCCGAGCCGGTGCTGT
ACCCGGATGTGGCGGTCAAGGTGGCGCGTGCCATCGAGGCGGGAGAGGTCGAACGTGGGGTGCTGGTGTGTGGTACCGGC
ATCGGGGTGGCGATTTCGGCCAACAAGGTGCGTGGTATTCGTGCCGCCCAGGCGCACGATACCTATTCGGCCGCCAAGGC
GCGCTCGAGCAACGATGCGCAGATCGTGACGCTGGGCGCTCGTGTGGTGGGCCCTGAACTGGCCAAGACCATCGTCACGA
CGTTCCTGGCGACCGAGTTCGCCGGCGGTGGCTCGCGCGAGAAAGTGGCGCGCATCCTGGCCTACGAGCGCGAAACCTTT
CGTGACCCGCCGCGCGTCTGA

Upstream 100 bases:

>100_bases
TAATTTTACAAATGTTCGTTAGGTGATATTTTGAACATGTCGTGATCGATTCAAGCGGATGATCGATCGCAGGTGGGAGG
ATTCGTTAGGGAGACGAGAC

Downstream 100 bases:

>100_bases
TCGCCGAGAGCACGCTTGTTCGCTGTGGTATCTTGTGCGCATCGTTCGAGCGGATCGCGTGAGGAGCAGTCATGAGTGAG
CGTGCCGAGAGCGCCGATGT

Product: ribose-5-phosphate isomerase

Products: D-Ribulose 5-phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MSDSRTASHTAPMRVALGGDEAAYELKALLIEHVRALGHEAVDFGTYDAEPVLYPDVAVKVARAIEAGEVERGVLVCGTG
IGVAISANKVRGIRAAQAHDTYSAAKARSSNDAQIVTLGARVVGPELAKTIVTTFLATEFAGGGSREKVARILAYERETF
RDPPRV

Sequences:

>Translated_166_residues
MSDSRTASHTAPMRVALGGDEAAYELKALLIEHVRALGHEAVDFGTYDAEPVLYPDVAVKVARAIEAGEVERGVLVCGTG
IGVAISANKVRGIRAAQAHDTYSAAKARSSNDAQIVTLGARVVGPELAKTIVTTFLATEFAGGGSREKVARILAYERETF
RDPPRV
>Mature_165_residues
SDSRTASHTAPMRVALGGDEAAYELKALLIEHVRALGHEAVDFGTYDAEPVLYPDVAVKVARAIEAGEVERGVLVCGTGI
GVAISANKVRGIRAAQAHDTYSAAKARSSNDAQIVTLGARVVGPELAKTIVTTFLATEFAGGGSREKVARILAYERETFR
DPPRV

Specific function: In Addition To Its Activity On D-Ribose 5-Phosphate It Probably Also Has Activity On D-Allose 6-Phosphate. [C]

COG id: COG0698

COG function: function code G; Ribose 5-phosphate isomerase RpiB

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lacAB/rpiB family [H]

Homologues:

Organism=Escherichia coli, GI1790528, Length=144, Percent_Identity=41.6666666666667, Blast_Score=120, Evalue=3e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003500
- InterPro:   IPR004785 [H]

Pfam domain/function: PF02502 LacAB_rpiB [H]

EC number: 5.3.1.6 [C]

Molecular weight: Translated: 17548; Mature: 17417

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSRTASHTAPMRVALGGDEAAYELKALLIEHVRALGHEAVDFGTYDAEPVLYPDVAVK
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEECHHHHHH
VARAIEAGEVERGVLVCGTGIGVAISANKVRGIRAAQAHDTYSAAKARSSNDAQIVTLGA
HHHHHHCCCCCCCEEEEECCCEEEEECCCCCCEEHHHCCCHHHHHHCCCCCCCEEEEECC
RVVGPELAKTIVTTFLATEFAGGGSREKVARILAYERETFRDPPRV
HHHCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SDSRTASHTAPMRVALGGDEAAYELKALLIEHVRALGHEAVDFGTYDAEPVLYPDVAVK
CCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCEECHHHHHH
VARAIEAGEVERGVLVCGTGIGVAISANKVRGIRAAQAHDTYSAAKARSSNDAQIVTLGA
HHHHHHCCCCCCCEEEEECCCEEEEECCCCCCEEHHHCCCHHHHHHCCCCCCCEEEEECC
RVVGPELAKTIVTTFLATEFAGGGSREKVARILAYERETFRDPPRV
HHHCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 4.4 {D-ribose} 0.83 {D-ribose} [C]

Substrates: alpha-D-Ribose 5-phosphate [C]

Specific reaction: alpha-D-Ribose 5-phosphate <==> D-Ribulose 5-phosphate [C]

General reaction: Intramolecular oxidoreduction; Isomerization [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]