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The map label for this gene is yiaO [H]

Identifier: 92112465

GI number: 92112465

Start: 369803

End: 370798

Strand: Direct

Name: yiaO [H]

Synonym: Csal_0332

Alternate gene names: 92112465

Gene position: 369803-370798 (Clockwise)

Preceding gene: 92112464

Following gene: 92112466

Centisome position: 10.0

GC content: 58.03

Gene sequence:

>996_bases
ATGAAAACGTTAAAAAGCCGTGCCCTATTAACCCTGACCGCTGGCGTGGCACTGGCCGCCAATGCCGGCGTGAGCGCCGC
TGAAACCTTGAAGCTCGCACACTTCGTATCGCCGGCGCATGTCGTCAACAGTGCCGTCGTTTATCCCCTCAAGGAGGGTG
TCGAGAAGCAGACGAACGGAGAGCTGGAAATCAAGGTCTATCCAGGAGGCGAGCTGGGTTCCGGCCCCACGGAACAATAC
CCGCGCGTGCTGCAAGGGGTGGCGGACATCGTCTGGGGATTGCCGGGGTACACCTCGTCGCAATTCAAGAAATCGATGGT
CGTGGAAATGCCCGGTGCCATTCCCGACGACATGCATGGCTACGACATGCTCTGGAATGCCTATGAAGACCAGTTGAAGA
GTGAGTTTCCCGGCACCAAGCCGCTGGCGCTCTGGGTCAGCGAGCCGAACATTTTCATTATGAAAGATGAAGAAATCCGC
ACGCCCGAAGATCTCGAAGGACTCAAGATTCGTGTCTCGGGAGAAATGACGGCGGCGCTGATCGATGCCTACGGCGGCAC
GCCCGTGCAAATGCCCGCCGGCGAGATTTACAACTCGCTGCAAACCGGTCTCATCGATGGCCTGGTGACCGGCGCCAGTG
CCGTCAACGACTTCAAGCTCGATGAAGTGGCCAATGTCTATTCGGTGGGGGCTCCCCTGGGCCACATCATGTTTTATCTG
GCCATGAATCAGAACAAATACGACAGCCTTTCCGCCGAATTCCAGCAAGCCATCGACGACAACAGTGGCGAAAAACTCTC
CCGCAGCGGGGAGCAAGGCTGGAATGCGCGTGCCAACGAAACCCTGAAGGCCATTCGCGAGGATGCCGACAACGAGGTGA
TCGAGCTTGACCCGGAAGAAATCGCCGCGTTCGAGCGCATTGCTGATGACTTCCGCGAGCAGCGTCTGGCGGAAAAGGGC
ACCAATGACGTGCTAGACGCCATGCGTGGGGAGTGA

Upstream 100 bases:

>100_bases
CCGGGTACGGTGCTTTCAGGCTCCGTTTCTTTATGCATTCTCGTGGGAAAACGGAGAATAAAATAAGACGAAAGTTTAAT
AAAAGTCGGGAGACGCCGGA

Downstream 100 bases:

>100_bases
ATTCCCGATGAGTCGCTTTCGCACGCGGGTGACCCGCACGCTGGATGGGCTGGTGAACCTGTCCAGCATCCTCGGCACCC
TGGCGCTGATCTTTGCGGTA

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKTLKSRALLTLTAGVALAANAGVSAAETLKLAHFVSPAHVVNSAVVYPLKEGVEKQTNGELEIKVYPGGELGSGPTEQY
PRVLQGVADIVWGLPGYTSSQFKKSMVVEMPGAIPDDMHGYDMLWNAYEDQLKSEFPGTKPLALWVSEPNIFIMKDEEIR
TPEDLEGLKIRVSGEMTAALIDAYGGTPVQMPAGEIYNSLQTGLIDGLVTGASAVNDFKLDEVANVYSVGAPLGHIMFYL
AMNQNKYDSLSAEFQQAIDDNSGEKLSRSGEQGWNARANETLKAIREDADNEVIELDPEEIAAFERIADDFREQRLAEKG
TNDVLDAMRGE

Sequences:

>Translated_331_residues
MKTLKSRALLTLTAGVALAANAGVSAAETLKLAHFVSPAHVVNSAVVYPLKEGVEKQTNGELEIKVYPGGELGSGPTEQY
PRVLQGVADIVWGLPGYTSSQFKKSMVVEMPGAIPDDMHGYDMLWNAYEDQLKSEFPGTKPLALWVSEPNIFIMKDEEIR
TPEDLEGLKIRVSGEMTAALIDAYGGTPVQMPAGEIYNSLQTGLIDGLVTGASAVNDFKLDEVANVYSVGAPLGHIMFYL
AMNQNKYDSLSAEFQQAIDDNSGEKLSRSGEQGWNARANETLKAIREDADNEVIELDPEEIAAFERIADDFREQRLAEKG
TNDVLDAMRGE
>Mature_331_residues
MKTLKSRALLTLTAGVALAANAGVSAAETLKLAHFVSPAHVVNSAVVYPLKEGVEKQTNGELEIKVYPGGELGSGPTEQY
PRVLQGVADIVWGLPGYTSSQFKKSMVVEMPGAIPDDMHGYDMLWNAYEDQLKSEFPGTKPLALWVSEPNIFIMKDEEIR
TPEDLEGLKIRVSGEMTAALIDAYGGTPVQMPAGEIYNSLQTGLIDGLVTGASAVNDFKLDEVANVYSVGAPLGHIMFYL
AMNQNKYDSLSAEFQQAIDDNSGEKLSRSGEQGWNARANETLKAIREDADNEVIELDPEEIAAFERIADDFREQRLAEKG
TNDVLDAMRGE

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=272, Percent_Identity=27.2058823529412, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36005; Mature: 36005

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLKSRALLTLTAGVALAANAGVSAAETLKLAHFVSPAHVVNSAVVYPLKEGVEKQTNG
CCCCHHCEEEEEECCHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHCCCC
ELEIKVYPGGELGSGPTEQYPRVLQGVADIVWGLPGYTSSQFKKSMVVEMPGAIPDDMHG
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEECCCCCCCCCCC
YDMLWNAYEDQLKSEFPGTKPLALWVSEPNIFIMKDEEIRTPEDLEGLKIRVSGEMTAAL
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCEEEEECCCHHHHH
IDAYGGTPVQMPAGEIYNSLQTGLIDGLVTGASAVNDFKLDEVANVYSVGAPLGHIMFYL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHCCCHHHHHHHH
AMNQNKYDSLSAEFQQAIDDNSGEKLSRSGEQGWNARANETLKAIREDADNEVIELDPEE
EECCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEECHHH
IAAFERIADDFREQRLAEKGTNDVLDAMRGE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MKTLKSRALLTLTAGVALAANAGVSAAETLKLAHFVSPAHVVNSAVVYPLKEGVEKQTNG
CCCCHHCEEEEEECCHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHCCCC
ELEIKVYPGGELGSGPTEQYPRVLQGVADIVWGLPGYTSSQFKKSMVVEMPGAIPDDMHG
EEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHEECCCCCCCCCCC
YDMLWNAYEDQLKSEFPGTKPLALWVSEPNIFIMKDEEIRTPEDLEGLKIRVSGEMTAAL
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEECCCCCCCCCCCCEEEEECCCHHHHH
IDAYGGTPVQMPAGEIYNSLQTGLIDGLVTGASAVNDFKLDEVANVYSVGAPLGHIMFYL
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCHHHHHHHHHHCCCHHHHHHHH
AMNQNKYDSLSAEFQQAIDDNSGEKLSRSGEQGWNARANETLKAIREDADNEVIELDPEE
EECCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEECHHH
IAAFERIADDFREQRLAEKGTNDVLDAMRGE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]