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The map label for this gene is prpB [C]

Identifier: 92112386

GI number: 92112386

Start: 285207

End: 286070

Strand: Direct

Name: prpB [C]

Synonym: Csal_0251

Alternate gene names: 92112386

Gene position: 285207-286070 (Clockwise)

Preceding gene: 92112385

Following gene: 92112387

Centisome position: 7.72

GC content: 66.2

Gene sequence:

>864_bases
GTGGCAACCCCCTCGCAACATGATCTGCGCAATGACTTTCGCGCCTTGCTGGCGTCCTCTGAGTGTTACTTCACCGCCTC
GGTGTTCGACCCGATGTCGGCGCGCATCGCCGCCGATCTGGGCTTCGAAGTGGGGATTCTGGGGGGCTCGGTGGCCTCGC
TTCAGGTGCTGGCGGCTCCCGATTTCGCCCTGATCACCTTGAGCGAGTTCGTCGAGCAGGCGACCCGCATCGGTCGAGTG
ACGCGCCTGCCGGTGATCGCCGATGCCGACCACGGCTATGGCAACGCGCTCAATGTCATGCGGACCATTACCGAGCTCGA
GCGTGCCGGTGTGGCGGCGCTGACCATCGAGGACACCCTGCTGCCGGCGCAGTATGGGCATAAGTCCACCGACCTGATCC
CCCTCGATGAAGGCGTGGGCAAGATGCGCGCGGCCCTGGAGGCGCGCATCGACCCGGCGATGGCGATCATCGCGCGTACC
AATGCCGGTCAGCTCGACGACGAGGCCGCCGTCGAGCGCGTCTGCGCCTATCAGGCGGCGGGTGTCGATGCCATCTGCCT
GGTGGGCGTGCGCGATTTCGACCACCTCGAGCGCCTGGCCGCGCCGCTGGACATTCCGCTGATGCTGGTCACCTACGGCA
ATCCCGAGCTGCGCGACCGCGCGCGTCTGGCCGCGCTGGGAGTGCGGGTCGTGGTCAATGGCCATGCGGCCTACTTCGCC
GCCATCAAGGCGACCTACGATTGCCTGCGCGAGCAGCGTGACATCGCGGCCAGCGAACTGAACGCCTCGCAATTGGCGAC
ACGCTACTCGACGCTCGACGAATACCGCGAATGGGCGCGCGACTATATGGACGTCAAGGAGTAG

Upstream 100 bases:

>100_bases
GGCGTCGTCGTTCACCGCCAGCCAGTCTGGCCATGTCCAACCCGGGTGCCGCCGCTTGCCAGCCAGGCGCGCGGTGCTTG
CGTGATAACAGGAGTTTTTA

Downstream 100 bases:

>100_bases
CGTTACGCTGACGAGCCACGAGCAGGGAATGGCTGGCCACGCCCATGGGTGGTTCAAGCTGTCGCCGCCTGCCGCTCGCG
GCCGTTGACCACCCGATACC

Product: 2,3-dimethylmalate lyase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MATPSQHDLRNDFRALLASSECYFTASVFDPMSARIAADLGFEVGILGGSVASLQVLAAPDFALITLSEFVEQATRIGRV
TRLPVIADADHGYGNALNVMRTITELERAGVAALTIEDTLLPAQYGHKSTDLIPLDEGVGKMRAALEARIDPAMAIIART
NAGQLDDEAAVERVCAYQAAGVDAICLVGVRDFDHLERLAAPLDIPLMLVTYGNPELRDRARLAALGVRVVVNGHAAYFA
AIKATYDCLREQRDIAASELNASQLATRYSTLDEYREWARDYMDVKE

Sequences:

>Translated_287_residues
MATPSQHDLRNDFRALLASSECYFTASVFDPMSARIAADLGFEVGILGGSVASLQVLAAPDFALITLSEFVEQATRIGRV
TRLPVIADADHGYGNALNVMRTITELERAGVAALTIEDTLLPAQYGHKSTDLIPLDEGVGKMRAALEARIDPAMAIIART
NAGQLDDEAAVERVCAYQAAGVDAICLVGVRDFDHLERLAAPLDIPLMLVTYGNPELRDRARLAALGVRVVVNGHAAYFA
AIKATYDCLREQRDIAASELNASQLATRYSTLDEYREWARDYMDVKE
>Mature_286_residues
ATPSQHDLRNDFRALLASSECYFTASVFDPMSARIAADLGFEVGILGGSVASLQVLAAPDFALITLSEFVEQATRIGRVT
RLPVIADADHGYGNALNVMRTITELERAGVAALTIEDTLLPAQYGHKSTDLIPLDEGVGKMRAALEARIDPAMAIIARTN
AGQLDDEAAVERVCAYQAAGVDAICLVGVRDFDHLERLAAPLDIPLMLVTYGNPELRDRARLAALGVRVVVNGHAAYFAA
IKATYDCLREQRDIAASELNASQLATRYSTLDEYREWARDYMDVKE

Specific function: Catalyzes the decarboxylation of oxaloacetate into pyruvate. Seems to play a role in maintaining cellular concentrations of bicarbonate and pyruvate

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Oxaloacetate decarboxylase family

Homologues:

Organism=Escherichia coli, GI1786525, Length=256, Percent_Identity=28.515625, Blast_Score=93, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OADC_CHRSD (Q1R0Z3)

Other databases:

- EMBL:   CP000285
- RefSeq:   YP_572314.1
- ProteinModelPortal:   Q1R0Z3
- SMR:   Q1R0Z3
- STRING:   Q1R0Z3
- GeneID:   4029196
- GenomeReviews:   CP000285_GR
- KEGG:   csa:Csal_0251
- NMPDR:   fig|290398.4.peg.93
- eggNOG:   COG2513
- HOGENOM:   HBG728656
- OMA:   KERILQK
- ProtClustDB:   CLSK868955
- BioCyc:   CSAL290398:CSAL_0251-MONOMER
- HAMAP:   MF_01299
- InterPro:   IPR000918
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60

Pfam domain/function: PF00463 ICL; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =4.1.1.3

Molecular weight: Translated: 31174; Mature: 31043

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: BINDING 50-50 BINDING 159-159 BINDING 235-235

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPSQHDLRNDFRALLASSECYFTASVFDPMSARIAADLGFEVGILGGSVASLQVLAAP
CCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCEEEEECCCHHEEEEEECC
DFALITLSEFVEQATRIGRVTRLPVIADADHGYGNALNVMRTITELERAGVAALTIEDTL
CHHHHHHHHHHHHHHHHCCCEECCEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEECCC
LPAQYGHKSTDLIPLDEGVGKMRAALEARIDPAMAIIARTNAGQLDDEAAVERVCAYQAA
CCHHCCCCCCCCEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHC
GVDAICLVGVRDFDHLERLAAPLDIPLMLVTYGNPELRDRARLAALGVRVVVNGHAAYFA
CCCEEEEEECCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCEEEEEECCHHHHH
AIKATYDCLREQRDIAASELNASQLATRYSTLDEYREWARDYMDVKE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATPSQHDLRNDFRALLASSECYFTASVFDPMSARIAADLGFEVGILGGSVASLQVLAAP
CCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHCCCEEEEECCCHHEEEEEECC
DFALITLSEFVEQATRIGRVTRLPVIADADHGYGNALNVMRTITELERAGVAALTIEDTL
CHHHHHHHHHHHHHHHHCCCEECCEEEECCCCCCHHHHHHHHHHHHHHCCEEEEEEECCC
LPAQYGHKSTDLIPLDEGVGKMRAALEARIDPAMAIIARTNAGQLDDEAAVERVCAYQAA
CCHHCCCCCCCCEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHC
GVDAICLVGVRDFDHLERLAAPLDIPLMLVTYGNPELRDRARLAALGVRVVVNGHAAYFA
CCCEEEEEECCCHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHCEEEEEECCHHHHH
AIKATYDCLREQRDIAASELNASQLATRYSTLDEYREWARDYMDVKE
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA