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The map label for this gene is phnE [H]

Identifier: 92112384

GI number: 92112384

Start: 283351

End: 284163

Strand: Direct

Name: phnE [H]

Synonym: Csal_0249

Alternate gene names: 92112384

Gene position: 283351-284163 (Clockwise)

Preceding gene: 92112383

Following gene: 92112385

Centisome position: 7.67

GC content: 64.7

Gene sequence:

>813_bases
ATGAACGAGGCGACACCCGCCTATCCCACGCACTGGCGGCGACCGGCGCGAATGCTGCATGACCGTCGCTGGCGGTGGCT
CGTGCAGGCGTCGGTGCTGGCCTACCTGGTCCTGGCGCTGGGGTCCTTCGACGTCGAGTGGGCGCGGGTGATGGAAGGAC
TGTCACGCGCGCAACGCTTCGCCCACGGATTTCTGCAACCCGATTTCACCAGCCGCTGGAGCGATATCCGCCAGGGACTC
ATCGAGAGCCTGACCATGACGCTCACCGCCACCGTGGTCGGGGTGGCGATCTCGGTGCCCATCGGCATCGGTGCCGCGCG
TAACCTGGTATCCGCGTGGATATATCGGTGCTGCCGGGCGATCATCGCCGTCAGCCGCTCGCTGCAGGAAATCATCGTGG
CGATCTTCCTGGTGGCAATGTTCGGCTTCGGTCCGCTGGCGGGGTTCGCGACGCTGTCGTTCGCCTCGATCGGCTTCATT
GCCAAGCTGCTGGCCGACGACATCGAAGAGGCCGACCCCCGTCAGGTGGAAGCCATTCGCGCCACCGGTGCGGGGTGGTG
GCAGGTGCTGCACTACGCCATTCAACCCCAGGTGATGCCGCGCCTGATCGGTTTGTCGCTCTACCGGCTGGACATCAACT
TCCGCGAAAGCGCGGTGATCGGCATCGTCGGCGCAGGGGGCATCGGCGCCACCCTCAATACCGCCATCGACCGTTACGAA
TACGGGAGTGCCGGCGCCATCCTGCTGATCGTCATCGCCATCGTGATGGTGGCGGAATACGGGTCTAGCTACCTGAGAAG
GTTCCTGCAATGA

Upstream 100 bases:

>100_bases
CCACTATCTACGGCGCCGAGGACTGGGCGTCGTCGGGCCGCGAGCCCGCTCCGGAACGGGAGCCCGAGGACACCGAACGG
CACCTGGCGGAGGTGGGGCG

Downstream 100 bases:

>100_bases
AGTCGACTTCCGCGAACGCCGGGGCGCCGGCCAGTCATGCCGACGCCTGGACCTTTCGCACGCCCCGGGCGCGGCTGGCC
CTGTGGTTCGGCTGGCTGGC

Product: phosphonate ABC transporter inner membrane protein

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MNEATPAYPTHWRRPARMLHDRRWRWLVQASVLAYLVLALGSFDVEWARVMEGLSRAQRFAHGFLQPDFTSRWSDIRQGL
IESLTMTLTATVVGVAISVPIGIGAARNLVSAWIYRCCRAIIAVSRSLQEIIVAIFLVAMFGFGPLAGFATLSFASIGFI
AKLLADDIEEADPRQVEAIRATGAGWWQVLHYAIQPQVMPRLIGLSLYRLDINFRESAVIGIVGAGGIGATLNTAIDRYE
YGSAGAILLIVIAIVMVAEYGSSYLRRFLQ

Sequences:

>Translated_270_residues
MNEATPAYPTHWRRPARMLHDRRWRWLVQASVLAYLVLALGSFDVEWARVMEGLSRAQRFAHGFLQPDFTSRWSDIRQGL
IESLTMTLTATVVGVAISVPIGIGAARNLVSAWIYRCCRAIIAVSRSLQEIIVAIFLVAMFGFGPLAGFATLSFASIGFI
AKLLADDIEEADPRQVEAIRATGAGWWQVLHYAIQPQVMPRLIGLSLYRLDINFRESAVIGIVGAGGIGATLNTAIDRYE
YGSAGAILLIVIAIVMVAEYGSSYLRRFLQ
>Mature_270_residues
MNEATPAYPTHWRRPARMLHDRRWRWLVQASVLAYLVLALGSFDVEWARVMEGLSRAQRFAHGFLQPDFTSRWSDIRQGL
IESLTMTLTATVVGVAISVPIGIGAARNLVSAWIYRCCRAIIAVSRSLQEIIVAIFLVAMFGFGPLAGFATLSFASIGFI
AKLLADDIEEADPRQVEAIRATGAGWWQVLHYAIQPQVMPRLIGLSLYRLDINFRESAVIGIVGAGGIGATLNTAIDRYE
YGSAGAILLIVIAIVMVAEYGSSYLRRFLQ

Specific function: Part of the ABC transporter complex phnCDE involved in phosphate import. Responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29803; Mature: 29803

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEATPAYPTHWRRPARMLHDRRWRWLVQASVLAYLVLALGSFDVEWARVMEGLSRAQRF
CCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AHGFLQPDFTSRWSDIRQGLIESLTMTLTATVVGVAISVPIGIGAARNLVSAWIYRCCRA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IIAVSRSLQEIIVAIFLVAMFGFGPLAGFATLSFASIGFIAKLLADDIEEADPRQVEAIR
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ATGAGWWQVLHYAIQPQVMPRLIGLSLYRLDINFRESAVIGIVGAGGIGATLNTAIDRYE
HCCHHHHHHHHHHHCHHHHHHHHHHHHEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHC
YGSAGAILLIVIAIVMVAEYGSSYLRRFLQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNEATPAYPTHWRRPARMLHDRRWRWLVQASVLAYLVLALGSFDVEWARVMEGLSRAQRF
CCCCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AHGFLQPDFTSRWSDIRQGLIESLTMTLTATVVGVAISVPIGIGAARNLVSAWIYRCCRA
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
IIAVSRSLQEIIVAIFLVAMFGFGPLAGFATLSFASIGFIAKLLADDIEEADPRQVEAIR
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
ATGAGWWQVLHYAIQPQVMPRLIGLSLYRLDINFRESAVIGIVGAGGIGATLNTAIDRYE
HCCHHHHHHHHHHHCHHHHHHHHHHHHEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHC
YGSAGAILLIVIAIVMVAEYGSSYLRRFLQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA