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The map label for this gene is glnA [H]

Identifier: 92112378

GI number: 92112378

Start: 276486

End: 277895

Strand: Direct

Name: glnA [H]

Synonym: Csal_0243

Alternate gene names: 92112378

Gene position: 276486-277895 (Clockwise)

Preceding gene: 92112377

Following gene: 92112379

Centisome position: 7.48

GC content: 61.77

Gene sequence:

>1410_bases
ATGTCTGAGAAGACCCTCGCCCTGATCGAAGAACACGATATCAAGTGGGTGGATCTGCGCTTTACCGATACCAAGGGCAA
GGAGCAGCACGTCACGATTCCGGCCGGCGCCGTCGATGATGAGTTCTTCGAGAACGGTCAGATGTTCGATGGCTCCTCCA
TTTCCGGCTGGAAGGGCATCAACGAGTCCGACATGATCCTGCGCCCGGAAGACGGCAGCGGCTACCTCGACCCGTTCACC
GAAGATGCCACGCTGGTGCTGCGCTGCGACATCATCGAGCCGGCCACCATGCAGGGGTACGAACGTGACCCGCGCTCCAT
CGCCAAGCGTGCCGAGGCGTACCTGAAGTCCACCGGTCTGGGCGATACCGCCTTCTTCGGCCCGGAACCTGAGTTCTTCA
TTTTCGACGAAGTGCACTGGAATGCCGACATCCAGGGCGCGATGTACAAGATCACGTCCGAGGAAGGCGCCTGGGCGACC
GATTCCACCACCGTGGAAGGCGGCAACCTGGGGCACCGTCCGCGCGTCAAGGGTGGCTACTTCCCGGTTCCGCCGGTGGA
CAGCTTCCACGACATCCGCAGCGCGATGTGCAACACCCTGGAAGCCATCGGCCAGTCGGTCGAGGTGCATCACCACGAAG
TCGCCAACGCCGGCCAGAACGAAATCGGTGTCAAGTTCAACACGCTGGTGAAGAAGGCCGACGAAGTTCAGGAAATGAAG
TACGTGATCCACAACGTGGCTCACGCCTACGGCAAGACCGCGACCTTCATGCCCAAGCCGCTGGTCGGTGACAACGGGTC
CGGCATGCACGTGCACCAGTCCTTCTGGAAGGACGGCACCAACATGTTCGCCGGTGACGAATACGCCGGGCTGTCCGAAA
CCGCGCTGTACTACATCGGCGGCATCATCAAGCACGCTCGTGCGCTGAACGCCTTCACCAACGCCTCGACCAACTCCTAC
AAGCGTCTGGTCCCGGGCTTCGAGGCGCCGGTCATGCTGGCCTACAGTGCCCGCAACCGCAGTGCCTCCATCCGCATTCC
GTACACCGCGAGCCCCAAGGGCAAGCGTGTGGAACTGCGCTTCCCGGATCCGACCGCCAACCCGTACCTGTGCTTCTCGG
CCATGCTGATGGCGGGTATCGACGGCATCAAGAACAAGATCCACCCCGGCGATGCGATGGACAAGAACCTCTATGACCTG
CCGCCGGAAGAAGGCAAGTCGGTTCCCACCGTCGCCAGCAGCCTCGACCAGGCCCTGGAAGCCTTGGATGCGGACCGCGC
CTTCCTGACCGAGGGCGACGTGTTCACCGACAGCATGATCGACGCCTATCTCGAGCTCAAGCAGGAAGAAGTCGACCGCC
TGCGCATGGCGACGCATCCGATCGAGTTCGACATGTACTACAGCTGCTGA

Upstream 100 bases:

>100_bases
GTAAACGCATACCCGTGGGCAGTCGTCGGCGGGCATGCTACAAACGACGCTGAACACGCAAGCAAACGACCCGAGCCGTG
GGCTCATAGTGGAGGAAATC

Downstream 100 bases:

>100_bases
TCCCGCGCGACCGCGACCGATCCCAGCCGTAGCGCTGATCGAGGCCCCGTCGGACGACGGGGCCTTTTACGTGGTCGTTC
GCGGGGCCGGGCGTGCGCTA

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSEKTLALIEEHDIKWVDLRFTDTKGKEQHVTIPAGAVDDEFFENGQMFDGSSISGWKGINESDMILRPEDGSGYLDPFT
EDATLVLRCDIIEPATMQGYERDPRSIAKRAEAYLKSTGLGDTAFFGPEPEFFIFDEVHWNADIQGAMYKITSEEGAWAT
DSTTVEGGNLGHRPRVKGGYFPVPPVDSFHDIRSAMCNTLEAIGQSVEVHHHEVANAGQNEIGVKFNTLVKKADEVQEMK
YVIHNVAHAYGKTATFMPKPLVGDNGSGMHVHQSFWKDGTNMFAGDEYAGLSETALYYIGGIIKHARALNAFTNASTNSY
KRLVPGFEAPVMLAYSARNRSASIRIPYTASPKGKRVELRFPDPTANPYLCFSAMLMAGIDGIKNKIHPGDAMDKNLYDL
PPEEGKSVPTVASSLDQALEALDADRAFLTEGDVFTDSMIDAYLELKQEEVDRLRMATHPIEFDMYYSC

Sequences:

>Translated_469_residues
MSEKTLALIEEHDIKWVDLRFTDTKGKEQHVTIPAGAVDDEFFENGQMFDGSSISGWKGINESDMILRPEDGSGYLDPFT
EDATLVLRCDIIEPATMQGYERDPRSIAKRAEAYLKSTGLGDTAFFGPEPEFFIFDEVHWNADIQGAMYKITSEEGAWAT
DSTTVEGGNLGHRPRVKGGYFPVPPVDSFHDIRSAMCNTLEAIGQSVEVHHHEVANAGQNEIGVKFNTLVKKADEVQEMK
YVIHNVAHAYGKTATFMPKPLVGDNGSGMHVHQSFWKDGTNMFAGDEYAGLSETALYYIGGIIKHARALNAFTNASTNSY
KRLVPGFEAPVMLAYSARNRSASIRIPYTASPKGKRVELRFPDPTANPYLCFSAMLMAGIDGIKNKIHPGDAMDKNLYDL
PPEEGKSVPTVASSLDQALEALDADRAFLTEGDVFTDSMIDAYLELKQEEVDRLRMATHPIEFDMYYSC
>Mature_468_residues
SEKTLALIEEHDIKWVDLRFTDTKGKEQHVTIPAGAVDDEFFENGQMFDGSSISGWKGINESDMILRPEDGSGYLDPFTE
DATLVLRCDIIEPATMQGYERDPRSIAKRAEAYLKSTGLGDTAFFGPEPEFFIFDEVHWNADIQGAMYKITSEEGAWATD
STTVEGGNLGHRPRVKGGYFPVPPVDSFHDIRSAMCNTLEAIGQSVEVHHHEVANAGQNEIGVKFNTLVKKADEVQEMKY
VIHNVAHAYGKTATFMPKPLVGDNGSGMHVHQSFWKDGTNMFAGDEYAGLSETALYYIGGIIKHARALNAFTNASTNSYK
RLVPGFEAPVMLAYSARNRSASIRIPYTASPKGKRVELRFPDPTANPYLCFSAMLMAGIDGIKNKIHPGDAMDKNLYDLP
PEEGKSVPTVASSLDQALEALDADRAFLTEGDVFTDSMIDAYLELKQEEVDRLRMATHPIEFDMYYSC

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=68.5897435897436, Blast_Score=705, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=291, Percent_Identity=25.4295532646048, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51961; Mature: 51830

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKTLALIEEHDIKWVDLRFTDTKGKEQHVTIPAGAVDDEFFENGQMFDGSSISGWKGI
CCCCEEEEEECCCCEEEEEEEECCCCCCEEEEECCCCCCHHHHCCCCEECCCCCCCCCCC
NESDMILRPEDGSGYLDPFTEDATLVLRCDIIEPATMQGYERDPRSIAKRAEAYLKSTGL
CCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCHHCCCCCCCHHHHHHHHHHHHHHCCC
GDTAFFGPEPEFFIFDEVHWNADIQGAMYKITSEEGAWATDSTTVEGGNLGHRPRVKGGY
CCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCEECCCCCCCCCCCCCCC
FPVPPVDSFHDIRSAMCNTLEAIGQSVEVHHHEVANAGQNEIGVKFNTLVKKADEVQEMK
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHH
YVIHNVAHAYGKTATFMPKPLVGDNGSGMHVHQSFWKDGTNMFAGDEYAGLSETALYYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
GIIKHARALNAFTNASTNSYKRLVPGFEAPVMLAYSARNRSASIRIPYTASPKGKRVELR
HHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCEEEEE
FPDPTANPYLCFSAMLMAGIDGIKNKIHPGDAMDKNLYDLPPEEGKSVPTVASSLDQALE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ALDADRAFLTEGDVFTDSMIDAYLELKQEEVDRLRMATHPIEFDMYYSC
HHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC
>Mature Secondary Structure 
SEKTLALIEEHDIKWVDLRFTDTKGKEQHVTIPAGAVDDEFFENGQMFDGSSISGWKGI
CCCEEEEEECCCCEEEEEEEECCCCCCEEEEECCCCCCHHHHCCCCEECCCCCCCCCCC
NESDMILRPEDGSGYLDPFTEDATLVLRCDIIEPATMQGYERDPRSIAKRAEAYLKSTGL
CCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCHHCCCCCCCHHHHHHHHHHHHHHCCC
GDTAFFGPEPEFFIFDEVHWNADIQGAMYKITSEEGAWATDSTTVEGGNLGHRPRVKGGY
CCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCEECCCCCCCCCCCCCCC
FPVPPVDSFHDIRSAMCNTLEAIGQSVEVHHHEVANAGQNEIGVKFNTLVKKADEVQEMK
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHH
YVIHNVAHAYGKTATFMPKPLVGDNGSGMHVHQSFWKDGTNMFAGDEYAGLSETALYYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCCCCCCCCCCCHHHHHHHHH
GIIKHARALNAFTNASTNSYKRLVPGFEAPVMLAYSARNRSASIRIPYTASPKGKRVELR
HHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEEEEECCCCCEEEEEEEECCCCCCEEEEE
FPDPTANPYLCFSAMLMAGIDGIKNKIHPGDAMDKNLYDLPPEEGKSVPTVASSLDQALE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
ALDADRAFLTEGDVFTDSMIDAYLELKQEEVDRLRMATHPIEFDMYYSC
HHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1969923 [H]