Click here to switch to the map view.

The map label for this gene is gloA [H]

Identifier: 92112352

GI number: 92112352

Start: 243171

End: 243788

Strand: Direct

Name: gloA [H]

Synonym: Csal_0217

Alternate gene names: 92112352

Gene position: 243171-243788 (Clockwise)

Preceding gene: 92112349

Following gene: 92112353

Centisome position: 6.58

GC content: 60.52

Gene sequence:

>618_bases
ATGCCCCCTGGAAATTTCATCGAGTGCCCTCATCTTCCCTCCCAACCGGACACCGATTCACATCCCCGCGATCAGGAGGA
CGCCATGAGCTTCCAGGGTGAACAGTATCCCGGCGTGCAGACGCCGTCGGCCGAAACCCAGGGGTTTCGTTTCAACCACA
CCATGCTGCGCATGAAGGATCCCGAGCGCTCGCTGGCGTTCTATTCGCGCGTCTTCGGCATGCGTGTGCTGCGTAAGCTC
GACTTCCCTGAGATGCAGTTCTCGCTGTACTTCCTGGCCAACGTGGACGACAACGACGCCGTGCCGGAAGACACCGCCGA
GCGCAATGTGTATACCTTTTCGCAAAAGGGCATCCTCGAGCTGACCCACAATTGGGGCACCGAGGATCAGGAAGGTTTCG
CCTACCACGACGGTAACGCCGAGCCCCAGGGCTTCGGGCATATCTGCTTCTCGGTACCGGACCTGCCCGCTGCGGTGGCG
TGGTTCGACGCCAACCAGGTCGAATTCAAGAAGCGTCCCGACGAGGGCAAGATGAAGGACGTGGTGTTCGTCAAAGACCC
CGACGGCTACTGGCTCGAGGTCGTTCAGCCGGAACGCTCCAGGGAGCTCGGCCAATAA

Upstream 100 bases:

>100_bases
AACGGCCGAGGCGATCAGAGAAAAAGCCAGATAAATATTAGTCTGCGAATTATCCCAGTCCGTGGGACATTTGTCTAATG
CTGCAGGCTCGCGCCAGCCG

Downstream 100 bases:

>100_bases
CGGCGCGCCGCATGGCCAGCGTCGAACGCCCGGTGTCGCATGGCACCGGGTTTTTTGTGCGTGTCCGCCAGGTGCGCGGT
TACGTTGAAAGTACGCAAGC

Product: glyoxalase I

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MPPGNFIECPHLPSQPDTDSHPRDQEDAMSFQGEQYPGVQTPSAETQGFRFNHTMLRMKDPERSLAFYSRVFGMRVLRKL
DFPEMQFSLYFLANVDDNDAVPEDTAERNVYTFSQKGILELTHNWGTEDQEGFAYHDGNAEPQGFGHICFSVPDLPAAVA
WFDANQVEFKKRPDEGKMKDVVFVKDPDGYWLEVVQPERSRELGQ

Sequences:

>Translated_205_residues
MPPGNFIECPHLPSQPDTDSHPRDQEDAMSFQGEQYPGVQTPSAETQGFRFNHTMLRMKDPERSLAFYSRVFGMRVLRKL
DFPEMQFSLYFLANVDDNDAVPEDTAERNVYTFSQKGILELTHNWGTEDQEGFAYHDGNAEPQGFGHICFSVPDLPAAVA
WFDANQVEFKKRPDEGKMKDVVFVKDPDGYWLEVVQPERSRELGQ
>Mature_204_residues
PPGNFIECPHLPSQPDTDSHPRDQEDAMSFQGEQYPGVQTPSAETQGFRFNHTMLRMKDPERSLAFYSRVFGMRVLRKLD
FPEMQFSLYFLANVDDNDAVPEDTAERNVYTFSQKGILELTHNWGTEDQEGFAYHDGNAEPQGFGHICFSVPDLPAAVAW
FDANQVEFKKRPDEGKMKDVVFVKDPDGYWLEVVQPERSRELGQ

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=158, Percent_Identity=51.8987341772152, Blast_Score=182, Evalue=1e-46,
Organism=Escherichia coli, GI1787940, Length=160, Percent_Identity=33.125, Blast_Score=83, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323639, Length=172, Percent_Identity=44.1860465116279, Blast_Score=147, Evalue=1e-36,
Organism=Drosophila melanogaster, GI20129743, Length=156, Percent_Identity=51.2820512820513, Blast_Score=167, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 23514; Mature: 23383

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00935 GLYOXALASE_I_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPGNFIECPHLPSQPDTDSHPRDQEDAMSFQGEQYPGVQTPSAETQGFRFNHTMLRMKD
CCCCCEEECCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEECCEEEEECC
PERSLAFYSRVFGMRVLRKLDFPEMQFSLYFLANVDDNDAVPEDTAERNVYTFSQKGILE
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEECCCCEEE
LTHNWGTEDQEGFAYHDGNAEPQGFGHICFSVPDLPAAVAWFDANQVEFKKRPDEGKMKD
EECCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCCEEEECCCCCCCCEE
VVFVKDPDGYWLEVVQPERSRELGQ
EEEEECCCCCEEEEECCHHHHCCCC
>Mature Secondary Structure 
PPGNFIECPHLPSQPDTDSHPRDQEDAMSFQGEQYPGVQTPSAETQGFRFNHTMLRMKD
CCCCEEECCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCEEECCEEEEECC
PERSLAFYSRVFGMRVLRKLDFPEMQFSLYFLANVDDNDAVPEDTAERNVYTFSQKGILE
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEECCCCEEE
LTHNWGTEDQEGFAYHDGNAEPQGFGHICFSVPDLPAAVAWFDANQVEFKKRPDEGKMKD
EECCCCCCCCCCEEEECCCCCCCCCEEEEEECCCCCEEEEEECCCCEEEECCCCCCCCEE
VVFVKDPDGYWLEVVQPERSRELGQ
EEEEECCCCCEEEEECCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]