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The map label for this gene is gsiB [H]

Identifier: 92112347

GI number: 92112347

Start: 235428

End: 237044

Strand: Direct

Name: gsiB [H]

Synonym: Csal_0212

Alternate gene names: 92112347

Gene position: 235428-237044 (Clockwise)

Preceding gene: 92112346

Following gene: 92112348

Centisome position: 6.37

GC content: 61.72

Gene sequence:

>1617_bases
ATGAACAACAAATTGAAAGGCTTCGCACTCACCGCTCTGTGCGCGACGATCTCGAGCCAGGCGCTGGCGCAACAGACACC
TCAGGAAGGCGGCGACATCGTGGTCACCTATCAGAACGACGTGGCCACGCTCGACCCGGCGATCGGCTACGACTGGCAAA
ACTGGTCGATGATCAAGAGCCTGTTCGACGGTCTGATAGACTACGAGCCGGGTACCACAGAGCTGAAGACGGACCTCGCC
AAGGACTACGAGATCTCCGACAATGGCCTGACCTACACCTTCCATCTGCGCGAAGGCGTTACCTTCCACAACGGCCGCGA
AATGGTTGCCAGCGACGTGAAGTACTCCCTGGAGCGGACGGTCAACCCCGAGACTCAGAGCCCCGGCGCCGGCTTCTTCT
CCTCCATCGAGGGCTTCGATGCCGTGGCCTCGGGCGAATCGATGGCGTTGAGCGGTATCACCACGCCCGACGATTACACC
GTGAAGATCCAGCTGTCGGCGCCGGATGCGGCTTTTCTGCACATCATGGCCCTCAACTTTGCCTCGGTGGTGCCCAAGGA
GTCGGTCGAGAAATGGGGCCGCGATTTCGGCAAGCATCCGGTGGGCACGGGGGCCTTCGAGTTGCAGGACTGGGCGCTCG
GCCAGCGTCTGACCTTCGTCAAGAATGACGACTACTACCGTGACGGTATTCCGTATCTCGATCGCATCGACTTCGAGATC
GGCCAGGAACCCAACGTGGCCCTGATGCGGTTGCAGCGCGGAGAGGTGGATATCGCTGGCGACGGCATCCCGCCCGCGCA
GTTCCTGCAATTCCGAAACGATCCCGAATTCAAGGATCTGATGGTCATCGGCGACCAACTGCATACCGGCTATTTGACCA
TGAACGTCACCATGCCTCCGCTGGATGAGGTCGAGGTGCGTCGTGCCATCAACATGGCGATCAACAAGGAGCGCATCGTG
CGCATCATCAATGGTCGCGCGATTCCTGCCAATCAGCCGCTGCCGCCGGCGATGCCCGGCTACGACGAGGAGTACGAGGG
CTACCCCTATGACGTGGCTCAGGCCAAGGCGCTTCTCGAGGAAGCCGGCTACGGCGATGGGTTCGATACCGAGATCTATG
TCATGAACACCGATCCACAGCCGCGTATCGCCCAGGCGATCCAGCAAGACTTGGCGCAGATCGGCGTACGTGCCGAAATC
AAGTCGCTGGCGCAGGCCAACGTGATCGCCGCGGGTGGCGACAAAGAGCAGGCGCCGATGGTGTGGTCGGGCGGCATGGC
GTGGATCGCCGATTTCCCCGACCCTTCCAACTTCTGGGGACCGATCCTGGGTTGCGAGGGCGCAGTGCCCGGCGGCTGGA
ACTGGGCGTGGTACTGCAACGAAGAGCTGGACGCCGAGGCTGACGCTGCCGACGCCATGGTCGCACCGGACCAGCAGCAG
GCACGCGCCGAGCGCTGGGGCAAGATATTCACCACGGCGATGGATGACGCGCCCTGGGTGCCGATCTTCAACGAGCAACG
CTTCACCGTGCACTCGGCACGCATGGGTGGCGATGATGCCTTGTACGTGGATCCGGTCCACGTTCCCGTCAACTACGACT
ACATCTGGGTGAAGTGA

Upstream 100 bases:

>100_bases
GTCGCCATCGTGCTGGGCAATCTCGTGGCCGATCTGGCCGTGCTGTTCGTCGATCCGCGCATCGACGTCACTCAAACCTG
AACCTCAAGGGAACAATGCC

Downstream 100 bases:

>100_bases
TGTGCGACAACGGATTGATCAAGACCATTCACGGTCATCACAGCCATCTGGGATGGGATAATGCCATCGCCCCGGCCGCG
CACGTCGCGCCGGGGACCCG

Product: extracellular solute-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MNNKLKGFALTALCATISSQALAQQTPQEGGDIVVTYQNDVATLDPAIGYDWQNWSMIKSLFDGLIDYEPGTTELKTDLA
KDYEISDNGLTYTFHLREGVTFHNGREMVASDVKYSLERTVNPETQSPGAGFFSSIEGFDAVASGESMALSGITTPDDYT
VKIQLSAPDAAFLHIMALNFASVVPKESVEKWGRDFGKHPVGTGAFELQDWALGQRLTFVKNDDYYRDGIPYLDRIDFEI
GQEPNVALMRLQRGEVDIAGDGIPPAQFLQFRNDPEFKDLMVIGDQLHTGYLTMNVTMPPLDEVEVRRAINMAINKERIV
RIINGRAIPANQPLPPAMPGYDEEYEGYPYDVAQAKALLEEAGYGDGFDTEIYVMNTDPQPRIAQAIQQDLAQIGVRAEI
KSLAQANVIAAGGDKEQAPMVWSGGMAWIADFPDPSNFWGPILGCEGAVPGGWNWAWYCNEELDAEADAADAMVAPDQQQ
ARAERWGKIFTTAMDDAPWVPIFNEQRFTVHSARMGGDDALYVDPVHVPVNYDYIWVK

Sequences:

>Translated_538_residues
MNNKLKGFALTALCATISSQALAQQTPQEGGDIVVTYQNDVATLDPAIGYDWQNWSMIKSLFDGLIDYEPGTTELKTDLA
KDYEISDNGLTYTFHLREGVTFHNGREMVASDVKYSLERTVNPETQSPGAGFFSSIEGFDAVASGESMALSGITTPDDYT
VKIQLSAPDAAFLHIMALNFASVVPKESVEKWGRDFGKHPVGTGAFELQDWALGQRLTFVKNDDYYRDGIPYLDRIDFEI
GQEPNVALMRLQRGEVDIAGDGIPPAQFLQFRNDPEFKDLMVIGDQLHTGYLTMNVTMPPLDEVEVRRAINMAINKERIV
RIINGRAIPANQPLPPAMPGYDEEYEGYPYDVAQAKALLEEAGYGDGFDTEIYVMNTDPQPRIAQAIQQDLAQIGVRAEI
KSLAQANVIAAGGDKEQAPMVWSGGMAWIADFPDPSNFWGPILGCEGAVPGGWNWAWYCNEELDAEADAADAMVAPDQQQ
ARAERWGKIFTTAMDDAPWVPIFNEQRFTVHSARMGGDDALYVDPVHVPVNYDYIWVK
>Mature_538_residues
MNNKLKGFALTALCATISSQALAQQTPQEGGDIVVTYQNDVATLDPAIGYDWQNWSMIKSLFDGLIDYEPGTTELKTDLA
KDYEISDNGLTYTFHLREGVTFHNGREMVASDVKYSLERTVNPETQSPGAGFFSSIEGFDAVASGESMALSGITTPDDYT
VKIQLSAPDAAFLHIMALNFASVVPKESVEKWGRDFGKHPVGTGAFELQDWALGQRLTFVKNDDYYRDGIPYLDRIDFEI
GQEPNVALMRLQRGEVDIAGDGIPPAQFLQFRNDPEFKDLMVIGDQLHTGYLTMNVTMPPLDEVEVRRAINMAINKERIV
RIINGRAIPANQPLPPAMPGYDEEYEGYPYDVAQAKALLEEAGYGDGFDTEIYVMNTDPQPRIAQAIQQDLAQIGVRAEI
KSLAQANVIAAGGDKEQAPMVWSGGMAWIADFPDPSNFWGPILGCEGAVPGGWNWAWYCNEELDAEADAADAMVAPDQQQ
ARAERWGKIFTTAMDDAPWVPIFNEQRFTVHSARMGGDDALYVDPVHVPVNYDYIWVK

Specific function: Part of the ABC transporter complex gsiABCD involved in glutathione import [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787052, Length=518, Percent_Identity=29.5366795366795, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI1789966, Length=470, Percent_Identity=30.8510638297872, Blast_Score=212, Evalue=6e-56,
Organism=Escherichia coli, GI1787551, Length=542, Percent_Identity=27.859778597786, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1787762, Length=488, Percent_Identity=29.0983606557377, Blast_Score=155, Evalue=6e-39,
Organism=Escherichia coli, GI1789397, Length=516, Percent_Identity=25.7751937984496, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1787495, Length=513, Percent_Identity=24.1715399610136, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI87081878, Length=515, Percent_Identity=25.631067961165, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1789887, Length=426, Percent_Identity=24.8826291079812, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59542; Mature: 59542

Theoretical pI: Translated: 4.12; Mature: 4.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNKLKGFALTALCATISSQALAQQTPQEGGDIVVTYQNDVATLDPAIGYDWQNWSMIKS
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEECCCCCCCCCCCHHHHHH
LFDGLIDYEPGTTELKTDLAKDYEISDNGLTYTFHLREGVTFHNGREMVASDVKYSLERT
HHHHHCCCCCCCHHHHHHHHCCCEECCCCEEEEEEECCCCEEECCHHHHHHHHHHHHHHC
VNPETQSPGAGFFSSIEGFDAVASGESMALSGITTPDDYTVKIQLSAPDAAFLHIMALNF
CCCCCCCCCCCHHHHCCCCHHHCCCCCEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHH
ASVVPKESVEKWGRDFGKHPVGTGAFELQDWALGQRLTFVKNDDYYRDGIPYLDRIDFEI
HHHCCHHHHHHHHHHHCCCCCCCCCEEEHHHHCCCEEEEEECCCCCCCCCCHHHHCCCCC
GQEPNVALMRLQRGEVDIAGDGIPPAQFLQFRNDPEFKDLMVIGDQLHTGYLTMNVTMPP
CCCCCCEEEEEECCCEEEECCCCCHHHHHHCCCCCCHHEEEEECCEEECCEEEEEEECCC
LDEVEVRRAINMAINKERIVRIINGRAIPANQPLPPAMPGYDEEYEGYPYDVAQAKALLE
CHHHHHHHHHHHHCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
EAGYGDGFDTEIYVMNTDPQPRIAQAIQQDLAQIGVRAEIKSLAQANVIAAGGDKEQAPM
HCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCEEEECCCCCCCCE
VWSGGMAWIADFPDPSNFWGPILGCEGAVPGGWNWAWYCNEELDAEADAADAMVAPDQQQ
EEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEECCCHHH
ARAERWGKIFTTAMDDAPWVPIFNEQRFTVHSARMGGDDALYVDPVHVPVNYDYIWVK
HHHHHHHHEEEEECCCCCCCEEECCCEEEEEECCCCCCCEEEEEEEEEEECCCEEEEC
>Mature Secondary Structure
MNNKLKGFALTALCATISSQALAQQTPQEGGDIVVTYQNDVATLDPAIGYDWQNWSMIKS
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEECCCCCCCCCCCHHHHHH
LFDGLIDYEPGTTELKTDLAKDYEISDNGLTYTFHLREGVTFHNGREMVASDVKYSLERT
HHHHHCCCCCCCHHHHHHHHCCCEECCCCEEEEEEECCCCEEECCHHHHHHHHHHHHHHC
VNPETQSPGAGFFSSIEGFDAVASGESMALSGITTPDDYTVKIQLSAPDAAFLHIMALNF
CCCCCCCCCCCHHHHCCCCHHHCCCCCEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHH
ASVVPKESVEKWGRDFGKHPVGTGAFELQDWALGQRLTFVKNDDYYRDGIPYLDRIDFEI
HHHCCHHHHHHHHHHHCCCCCCCCCEEEHHHHCCCEEEEEECCCCCCCCCCHHHHCCCCC
GQEPNVALMRLQRGEVDIAGDGIPPAQFLQFRNDPEFKDLMVIGDQLHTGYLTMNVTMPP
CCCCCCEEEEEECCCEEEECCCCCHHHHHHCCCCCCHHEEEEECCEEECCEEEEEEECCC
LDEVEVRRAINMAINKERIVRIINGRAIPANQPLPPAMPGYDEEYEGYPYDVAQAKALLE
CHHHHHHHHHHHHCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
EAGYGDGFDTEIYVMNTDPQPRIAQAIQQDLAQIGVRAEIKSLAQANVIAAGGDKEQAPM
HCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHHHHCCEEEECCCCCCCCE
VWSGGMAWIADFPDPSNFWGPILGCEGAVPGGWNWAWYCNEELDAEADAADAMVAPDQQQ
EEECCEEEEECCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCEECCCHHH
ARAERWGKIFTTAMDDAPWVPIFNEQRFTVHSARMGGDDALYVDPVHVPVNYDYIWVK
HHHHHHHHEEEEECCCCCCCEEECCCEEEEEECCCCCCCEEEEEEEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA