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The map label for this gene is yiaO [H]

Identifier: 91790406

GI number: 91790406

Start: 4847436

End: 4848470

Strand: Reverse

Name: yiaO [H]

Synonym: Bpro_4577

Alternate gene names: 91790406

Gene position: 4848470-4847436 (Counterclockwise)

Preceding gene: 91790408

Following gene: 91790405

Centisome position: 93.24

GC content: 65.51

Gene sequence:

>1035_bases
ATGCATTCACATTCACTTTCCCGCCGCCGCTTTGTCCAGTCCACCGCCGCCGCCTCCGTGGCGCTTGCCTCCGGCTTGCC
CAGCCTGGCGCGGGCGCAGGCGCCCGTTGCGCTGCGCTTTTCGTCCTCGCTGGTCGCCGACCAGAATTCGGCCCACTACG
TCTGGTACCAGCGCCTGGACGCCAACCTGAAGGCCGCCGTCGGCGACCGGATCCGGATCGACTACTTTCCCAACAACCAG
CTCGGCAAGGAAAGCGATGTGGTGCAGCAGGTCAAGGTCGGCTCGATTGACATGATGGTCACCGGCTCGTCGATCTGGGC
CACGGCCGCGCCGCAGTTCGGCATGCTGGACCTCGGTTACCTGTTTGACAGCTATGCCCATGCGGCCAGGGCGCTGGACG
GGGGCGTGGGCGCCAAACTCAATGACATCCTGCAAAAGAGCACGGGCTGCCAGGTCATCACCTGGGCTTCGCACTTTGGG
GCGCGCAGCGTGTACACCCGGCAGCCGGTCAAGTCGCTCGCCGAGGTGAAAAACGTCAAGCTGCGCGTGCTGCCCACGCC
GGCCTTCATTGAAACCTTCAAGATCATGGGCGCCATCCCGACGCCGATCCCGTTCGGTGAGCTCTACATGGCCGCGCAGA
CCGGTGTGGTGGACGGCTTCGAGCATGACGCCGCCACCGTGCTGGCCAGCAAGCTCAATGAAGTTGCCAAAAACTGCTGG
CTGACCGAACACCTCTTCAGCCCGCTGGTGGTGGTGATGGGCAAGCGCGGCATGGACAAGATCCCGGCAGACCTGCGCCC
GGCCTTCCTGAAGGCCGCGGCGGACGCCACCGCCCAGCAGCGCGCCATTGCCACCGACAAGGGCGCGCAGGCTATCGACG
CGCTAACGAAGCTGGGCATGGTGTTCAACCCGATGGCCAAAGCCGAGCGCGATGCCGTGCGCAAGGACATGGAGGCCCGT
CTCTGGTCCTCCTTTGCCAAGGAGCATCCGGCCACCGCGCCGCTGTTCACCGCGATCTCCGCGGCGAGGGTGTGA

Upstream 100 bases:

>100_bases
GACCGGCGAATTGGTGTAACGATACAGTAAATCGTTATACAAGCGCCCCACCGATGCACGCCAATGCATCCCTACAACGT
CAAGTCATCAGGAGACAACC

Downstream 100 bases:

>100_bases
GTCGTGAGCGCCCAGTTGTCGCCGGACCTGCCGCATATCCCCCTGCCTTCTGTCACCGGCTTCAGCGTGGCCCGCGCGCC
CGGGCGCTGGCTGGCCACCC

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MHSHSLSRRRFVQSTAAASVALASGLPSLARAQAPVALRFSSSLVADQNSAHYVWYQRLDANLKAAVGDRIRIDYFPNNQ
LGKESDVVQQVKVGSIDMMVTGSSIWATAAPQFGMLDLGYLFDSYAHAARALDGGVGAKLNDILQKSTGCQVITWASHFG
ARSVYTRQPVKSLAEVKNVKLRVLPTPAFIETFKIMGAIPTPIPFGELYMAAQTGVVDGFEHDAATVLASKLNEVAKNCW
LTEHLFSPLVVVMGKRGMDKIPADLRPAFLKAAADATAQQRAIATDKGAQAIDALTKLGMVFNPMAKAERDAVRKDMEAR
LWSSFAKEHPATAPLFTAISAARV

Sequences:

>Translated_344_residues
MHSHSLSRRRFVQSTAAASVALASGLPSLARAQAPVALRFSSSLVADQNSAHYVWYQRLDANLKAAVGDRIRIDYFPNNQ
LGKESDVVQQVKVGSIDMMVTGSSIWATAAPQFGMLDLGYLFDSYAHAARALDGGVGAKLNDILQKSTGCQVITWASHFG
ARSVYTRQPVKSLAEVKNVKLRVLPTPAFIETFKIMGAIPTPIPFGELYMAAQTGVVDGFEHDAATVLASKLNEVAKNCW
LTEHLFSPLVVVMGKRGMDKIPADLRPAFLKAAADATAQQRAIATDKGAQAIDALTKLGMVFNPMAKAERDAVRKDMEAR
LWSSFAKEHPATAPLFTAISAARV
>Mature_344_residues
MHSHSLSRRRFVQSTAAASVALASGLPSLARAQAPVALRFSSSLVADQNSAHYVWYQRLDANLKAAVGDRIRIDYFPNNQ
LGKESDVVQQVKVGSIDMMVTGSSIWATAAPQFGMLDLGYLFDSYAHAARALDGGVGAKLNDILQKSTGCQVITWASHFG
ARSVYTRQPVKSLAEVKNVKLRVLPTPAFIETFKIMGAIPTPIPFGELYMAAQTGVVDGFEHDAATVLASKLNEVAKNCW
LTEHLFSPLVVVMGKRGMDKIPADLRPAFLKAAADATAQQRAIATDKGAQAIDALTKLGMVFNPMAKAERDAVRKDMEAR
LWSSFAKEHPATAPLFTAISAARV

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=304, Percent_Identity=30.2631578947368, Blast_Score=148, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 37130; Mature: 37130

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSHSLSRRRFVQSTAAASVALASGLPSLARAQAPVALRFSSSLVADQNSAHYVWYQRLD
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECCHHHCCCCCCEEEEEEHHC
ANLKAAVGDRIRIDYFPNNQLGKESDVVQQVKVGSIDMMVTGSSIWATAAPQFGMLDLGY
CCHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCHHHHH
LFDSYAHAARALDGGVGAKLNDILQKSTGCQVITWASHFGARSVYTRQPVKSLAEVKNVK
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHCCE
LRVLPTPAFIETFKIMGAIPTPIPFGELYMAAQTGVVDGFEHDAATVLASKLNEVAKNCW
EEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LTEHLFSPLVVVMGKRGMDKIPADLRPAFLKAAADATAQQRAIATDKGAQAIDALTKLGM
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VFNPMAKAERDAVRKDMEARLWSSFAKEHPATAPLFTAISAARV
HHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MHSHSLSRRRFVQSTAAASVALASGLPSLARAQAPVALRFSSSLVADQNSAHYVWYQRLD
CCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEEEECCHHHCCCCCCEEEEEEHHC
ANLKAAVGDRIRIDYFPNNQLGKESDVVQQVKVGSIDMMVTGSSIWATAAPQFGMLDLGY
CCHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCEEEEEECCCEEECCCCCCCCHHHHH
LFDSYAHAARALDGGVGAKLNDILQKSTGCQVITWASHFGARSVYTRQPVKSLAEVKNVK
HHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHCCE
LRVLPTPAFIETFKIMGAIPTPIPFGELYMAAQTGVVDGFEHDAATVLASKLNEVAKNCW
EEEECCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LTEHLFSPLVVVMGKRGMDKIPADLRPAFLKAAADATAQQRAIATDKGAQAIDALTKLGM
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VFNPMAKAERDAVRKDMEARLWSSFAKEHPATAPLFTAISAARV
HHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]