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The map label for this gene is purR [H]

Identifier: 91790402

GI number: 91790402

Start: 4843131

End: 4844183

Strand: Reverse

Name: purR [H]

Synonym: Bpro_4573

Alternate gene names: 91790402

Gene position: 4844183-4843131 (Counterclockwise)

Preceding gene: 91790403

Following gene: 91790401

Centisome position: 93.15

GC content: 66.67

Gene sequence:

>1053_bases
GTGAGCAAGATTCAGGATGTGGCCAGCCGTGCTGGCGTGTCAACCAGCACGGTGTCCAACGTGCTCAATGGCCGGAGCAA
CCGCATGGCCAAAGAGACGCTGGCGCGTGTGGAGGCGGCGATTGCCGAGCTCCGGTACCGGCCCAACACCACGGCGCGCC
AGCTCAAGACCGGCCACACGCCCATGCTGGGTTTGCTGGTGCCGTCAATCGCCAACCCCATGTACGGCTTCATTGCCCGT
GAGATCGAAACCCAGGCACAGGAGCGCTACGGCTTTCGCATCATGATGGGCAACACCTATCGCGACAAGGACAAGGAAGC
CCGCTTCTTCGACGACCTGATGGCGCATGGCGTGCGCGGCGTGATCATCATCTCCTCGCTCGTCGACGAGCAGCACTTCG
AATCCGCCGGCGAGCGCGGCCTGGTGGTGGTCAGTTATGACCGCCGCGCCACGCCTGGCGTCGAGTCGGGCATTGACCAC
GTGTCGGTCGACAACTTCGAGGCGGCCCGCATCGCCACCGAGCACCTGATAGCGCAGGGCCACAGGCGGCTGGCCTTTGT
GACGGCCGCCGGCATCACCATGAGCCGGCGTGAAAAGATCAACGGTTTCATGGCGGCCGCGGCCAGCGCCGGCCTGGTTG
CCAGCGCGCAGGTGATCGAGGGCACGCCGCTCAATGAATACGGCGACTCGATGATGAGCGAGCTGGGCCGCATCGAGGCG
GCAAAGCTCGCCGGACGCGACCCGGCGGAGCGGCCCACCGGCGTTGTGGCGGTCAACGACATGATGGCGCTGGGCCTGAT
GGCCGGTTTTCGCGAGGCCGGGCTGTCCGTGCCGGCCGACGTGTCGGTGGTGGGCATGGACGATGTGTTTTTGTCGGCGC
TGATGCATCCGGCCCTGACCACGGTGCGCCTGCCCGTGCCGGAGATGGCCCACACCATCGTCGAGCGCGTGATGAGCCGG
CTGGCCGCCCCCAGCCTGCCGACCGGCGAGTTCATCTTCCAGCCCAGCCTGGTGGTCAGGGGCTCCGTGGCAGCCCGAAG
CTCCGAACAGTGA

Upstream 100 bases:

>100_bases
GTATCTTGTGGTCTTGTTTGTGACGCTGATGGTGCTGGTTCTGGTGCCGGCGTTTTCCCTGTGGCTGCCCACCCGCATGG
GCTTATAGGAAAGAAACCGC

Downstream 100 bases:

>100_bases
AGTCCTTTTGAGCATTGAATTTTTTGTGAACTTTTGACGATTGAACAATTGAACAAGGCATGGAATGACAACGGTTTTTG
TAAGCCACCCCCGAACCAGC

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 350; Mature: 349

Protein sequence:

>350_residues
MSKIQDVASRAGVSTSTVSNVLNGRSNRMAKETLARVEAAIAELRYRPNTTARQLKTGHTPMLGLLVPSIANPMYGFIAR
EIETQAQERYGFRIMMGNTYRDKDKEARFFDDLMAHGVRGVIIISSLVDEQHFESAGERGLVVVSYDRRATPGVESGIDH
VSVDNFEAARIATEHLIAQGHRRLAFVTAAGITMSRREKINGFMAAAASAGLVASAQVIEGTPLNEYGDSMMSELGRIEA
AKLAGRDPAERPTGVVAVNDMMALGLMAGFREAGLSVPADVSVVGMDDVFLSALMHPALTTVRLPVPEMAHTIVERVMSR
LAAPSLPTGEFIFQPSLVVRGSVAARSSEQ

Sequences:

>Translated_350_residues
MSKIQDVASRAGVSTSTVSNVLNGRSNRMAKETLARVEAAIAELRYRPNTTARQLKTGHTPMLGLLVPSIANPMYGFIAR
EIETQAQERYGFRIMMGNTYRDKDKEARFFDDLMAHGVRGVIIISSLVDEQHFESAGERGLVVVSYDRRATPGVESGIDH
VSVDNFEAARIATEHLIAQGHRRLAFVTAAGITMSRREKINGFMAAAASAGLVASAQVIEGTPLNEYGDSMMSELGRIEA
AKLAGRDPAERPTGVVAVNDMMALGLMAGFREAGLSVPADVSVVGMDDVFLSALMHPALTTVRLPVPEMAHTIVERVMSR
LAAPSLPTGEFIFQPSLVVRGSVAARSSEQ
>Mature_349_residues
SKIQDVASRAGVSTSTVSNVLNGRSNRMAKETLARVEAAIAELRYRPNTTARQLKTGHTPMLGLLVPSIANPMYGFIARE
IETQAQERYGFRIMMGNTYRDKDKEARFFDDLMAHGVRGVIIISSLVDEQHFESAGERGLVVVSYDRRATPGVESGIDHV
SVDNFEAARIATEHLIAQGHRRLAFVTAAGITMSRREKINGFMAAAASAGLVASAQVIEGTPLNEYGDSMMSELGRIEAA
KLAGRDPAERPTGVVAVNDMMALGLMAGFREAGLSVPADVSVVGMDDVFLSALMHPALTTVRLPVPEMAHTIVERVMSRL
AAPSLPTGEFIFQPSLVVRGSVAARSSEQ

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789202, Length=353, Percent_Identity=31.4447592067989, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1790194, Length=345, Percent_Identity=32.463768115942, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1788474, Length=352, Percent_Identity=30.6818181818182, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1787948, Length=348, Percent_Identity=29.3103448275862, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790369, Length=345, Percent_Identity=27.2463768115942, Blast_Score=131, Evalue=6e-32,
Organism=Escherichia coli, GI1787906, Length=330, Percent_Identity=29.3939393939394, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1789068, Length=354, Percent_Identity=29.9435028248588, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI48994940, Length=325, Percent_Identity=26.7692307692308, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1786540, Length=347, Percent_Identity=29.1066282420749, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787580, Length=330, Percent_Identity=25.4545454545455, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1786268, Length=149, Percent_Identity=32.2147651006711, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790715, Length=333, Percent_Identity=24.6246246246246, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1789456, Length=366, Percent_Identity=26.2295081967213, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37700; Mature: 37569

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIQDVASRAGVSTSTVSNVLNGRSNRMAKETLARVEAAIAELRYRPNTTARQLKTGHT
CCHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCC
PMLGLLVPSIANPMYGFIAREIETQAQERYGFRIMMGNTYRDKDKEARFFDDLMAHGVRG
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHCCCCH
VIIISSLVDEQHFESAGERGLVVVSYDRRATPGVESGIDHVSVDNFEAARIATEHLIAQG
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCC
HRRLAFVTAAGITMSRREKINGFMAAAASAGLVASAQVIEGTPLNEYGDSMMSELGRIEA
CCEEEEEEECCCHHHHHHHHHHHHHHHHHCCHHEEHHHHCCCCCHHHHHHHHHHHHHHHH
AKLAGRDPAERPTGVVAVNDMMALGLMAGFREAGLSVPADVSVVGMDDVFLSALMHPALT
HHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHH
TVRLPVPEMAHTIVERVMSRLAAPSLPTGEFIFQPSLVVRGSVAARSSEQ
EEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEECCCCCCCCCC
>Mature Secondary Structure 
SKIQDVASRAGVSTSTVSNVLNGRSNRMAKETLARVEAAIAELRYRPNTTARQLKTGHT
CHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCC
PMLGLLVPSIANPMYGFIAREIETQAQERYGFRIMMGNTYRDKDKEARFFDDLMAHGVRG
CHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHCCCCH
VIIISSLVDEQHFESAGERGLVVVSYDRRATPGVESGIDHVSVDNFEAARIATEHLIAQG
HHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHCC
HRRLAFVTAAGITMSRREKINGFMAAAASAGLVASAQVIEGTPLNEYGDSMMSELGRIEA
CCEEEEEEECCCHHHHHHHHHHHHHHHHHCCHHEEHHHHCCCCCHHHHHHHHHHHHHHHH
AKLAGRDPAERPTGVVAVNDMMALGLMAGFREAGLSVPADVSVVGMDDVFLSALMHPALT
HHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHH
TVRLPVPEMAHTIVERVMSRLAAPSLPTGEFIFQPSLVVRGSVAARSSEQ
EEECCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA