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The map label for this gene is ltaA [H]

Identifier: 91790287

GI number: 91790287

Start: 4707770

End: 4708882

Strand: Reverse

Name: ltaA [H]

Synonym: Bpro_4454

Alternate gene names: 91790287

Gene position: 4708882-4707770 (Counterclockwise)

Preceding gene: 91790288

Following gene: 91790286

Centisome position: 90.55

GC content: 67.03

Gene sequence:

>1113_bases
ATGGTTGTTGATCTGCGCAGCGACACCGTCACCCAGCCCAGCGCCGCGATGCGTGCAGCCATGCAGGCCGCGCCGCTGGG
CGACGACGTGTTTGGCGACGACCCCAGCGTCAACGCGCTGCAGGAGAAAATTGCCGGCATGCTGGGCTTTGAGGCCGCGC
TGTTTGTACCCACCGGCACGCAGAGCAACCTCTGCGCGATCTTGAGCCACTGCCAGCGCGGCGACGAATACATCGTCGGC
CAGATGGCGCACTGCTACCGCTGGGAAGGCGGCGGTGCGGCGGTGTTTGGCAGCGTGCAGCCGCAGCCGCTGAACCACCA
GCCCGACGGCACGCTGGCGGTCGCCGAGATCGAAGCTGCCATCAAGCCCGATGACCCGCATTTTGCGATCACCCGACTGC
TGGCACTGGAAAACACGCTGGGCGGCAAGCTGTTGCCGTTTGGCTATGTGCAGCAGGCCACGCAGTTGGCGCAGGCCAAA
GGCCTGAGCCGCCATCTGGACGGCGCGCGGCTGTTCAACGCCGCCGTGGCGCAAGCCGCACTGTCCGTTCGGGCTGAGCT
TGTCGAAGCCGCTGCAGGCACTTCGACAGGCTCAGTGCGAACGGAAGTTACCCGCGACAGTGCCGTCGCCGAAGCCAGGC
GCATCGCCCAGTGTTTCGACAGCGTGTCGGTGTGTTTCAGCAAGGGGCTGGGCGCGCCCGTGGGCTCCGCGCTGTGCGGC
TCGCGCGAGTTCATTGCGCGGGCGCGCCGCGTCCGCAAGATGGCCGGCGGCGCGATGCGCCAGTCCGGCATGCTGGCGGC
CGCCGCCAGCTACGCGCTGGACCATCACATTGAACGCCTGGCGCAGGACCATGCGCTGGCCCGGCAACTGGCCGACGGTC
TGGCGGGGATTGAGGGCTTGCACGTGGAAGCGCCCGAGACCAATATCCTCTTTGTCGACCTGAGCGGCGCGGCCCGCGAC
AAATCTGCCGCCCTGCTCAAGTACCTGGCCGATCACGGCATTCAGGCCACGGGCCTGTATCGCCTGCGTTTTGTCACGCA
CCTCGATGTGGACGCTGCCGGTGTGGACCGCGCCGTGGCCGCGATCCGGCAGTTCTTCCATCTCAAAGGATAA

Upstream 100 bases:

>100_bases
CCATGCCATGCTGAAGAACTTCCTGGAGCAGCGGGCATGACCTCGTTTGCCCTGAATTTGATAGCTGAAAGCTCGCCAAT
TTATTGGAAGGGAGGCCGAA

Downstream 100 bases:

>100_bases
ACAAGGATAAAGCCATGCCTGACTTTCAACACCTGTTGCTTTTTATCGCGGCCGGTTGGTTGCTTAATCTGACGCCGGGC
CCCGACGTGCTTTACATCGT

Product: L-threonine aldolase

Products: glycine; acetaldehyde

Alternate protein names: L-allo-TA; L-allo-threonine acetaldehyde-lyase [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MVVDLRSDTVTQPSAAMRAAMQAAPLGDDVFGDDPSVNALQEKIAGMLGFEAALFVPTGTQSNLCAILSHCQRGDEYIVG
QMAHCYRWEGGGAAVFGSVQPQPLNHQPDGTLAVAEIEAAIKPDDPHFAITRLLALENTLGGKLLPFGYVQQATQLAQAK
GLSRHLDGARLFNAAVAQAALSVRAELVEAAAGTSTGSVRTEVTRDSAVAEARRIAQCFDSVSVCFSKGLGAPVGSALCG
SREFIARARRVRKMAGGAMRQSGMLAAAASYALDHHIERLAQDHALARQLADGLAGIEGLHVEAPETNILFVDLSGAARD
KSAALLKYLADHGIQATGLYRLRFVTHLDVDAAGVDRAVAAIRQFFHLKG

Sequences:

>Translated_370_residues
MVVDLRSDTVTQPSAAMRAAMQAAPLGDDVFGDDPSVNALQEKIAGMLGFEAALFVPTGTQSNLCAILSHCQRGDEYIVG
QMAHCYRWEGGGAAVFGSVQPQPLNHQPDGTLAVAEIEAAIKPDDPHFAITRLLALENTLGGKLLPFGYVQQATQLAQAK
GLSRHLDGARLFNAAVAQAALSVRAELVEAAAGTSTGSVRTEVTRDSAVAEARRIAQCFDSVSVCFSKGLGAPVGSALCG
SREFIARARRVRKMAGGAMRQSGMLAAAASYALDHHIERLAQDHALARQLADGLAGIEGLHVEAPETNILFVDLSGAARD
KSAALLKYLADHGIQATGLYRLRFVTHLDVDAAGVDRAVAAIRQFFHLKG
>Mature_370_residues
MVVDLRSDTVTQPSAAMRAAMQAAPLGDDVFGDDPSVNALQEKIAGMLGFEAALFVPTGTQSNLCAILSHCQRGDEYIVG
QMAHCYRWEGGGAAVFGSVQPQPLNHQPDGTLAVAEIEAAIKPDDPHFAITRLLALENTLGGKLLPFGYVQQATQLAQAK
GLSRHLDGARLFNAAVAQAALSVRAELVEAAAGTSTGSVRTEVTRDSAVAEARRIAQCFDSVSVCFSKGLGAPVGSALCG
SREFIARARRVRKMAGGAMRQSGMLAAAASYALDHHIERLAQDHALARQLADGLAGIEGLHVEAPETNILFVDLSGAARD
KSAALLKYLADHGIQATGLYRLRFVTHLDVDAAGVDRAVAAIRQFFHLKG

Specific function: Stereospecifically catalyzes the interconversion of L- allo-threonine and glycine [H]

COG id: COG2008

COG function: function code E; Threonine aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1787095, Length=365, Percent_Identity=47.6712328767123, Blast_Score=323, Evalue=1e-89,
Organism=Caenorhabditis elegans, GI193206522, Length=376, Percent_Identity=39.8936170212766, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI193206524, Length=376, Percent_Identity=39.8936170212766, Blast_Score=231, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI193206520, Length=376, Percent_Identity=39.8936170212766, Blast_Score=230, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6320789, Length=368, Percent_Identity=33.1521739130435, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI21355439, Length=329, Percent_Identity=40.7294832826748, Blast_Score=237, Evalue=8e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01212 Beta_elim_lyase [H]

EC number: 4.1.2.5

Molecular weight: Translated: 39006; Mature: 39006

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVDLRSDTVTQPSAAMRAAMQAAPLGDDVFGDDPSVNALQEKIAGMLGFEAALFVPTGT
CEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCC
QSNLCAILSHCQRGDEYIVGQMAHCYRWEGGGAAVFGSVQPQPLNHQPDGTLAVAEIEAA
CCHHHHHHHHHHCCCHHHHHHHHHHEEECCCCEEEECCCCCCCCCCCCCCCEEEEEEHHH
IKPDDPHFAITRLLALENTLGGKLLPFGYVQQATQLAQAKGLSRHLDGARLFNAAVAQAA
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHH
LSVRAELVEAAAGTSTGSVRTEVTRDSAVAEARRIAQCFDSVSVCFSKGLGAPVGSALCG
HHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCC
SREFIARARRVRKMAGGAMRQSGMLAAAASYALDHHIERLAQDHALARQLADGLAGIEGL
CHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
HVEAPETNILFVDLSGAARDKSAALLKYLADHGIQATGLYRLRFVTHLDVDAAGVDRAVA
EEECCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHHHHHHH
AIRQFFHLKG
HHHHHHHCCC
>Mature Secondary Structure
MVVDLRSDTVTQPSAAMRAAMQAAPLGDDVFGDDPSVNALQEKIAGMLGFEAALFVPTGT
CEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCC
QSNLCAILSHCQRGDEYIVGQMAHCYRWEGGGAAVFGSVQPQPLNHQPDGTLAVAEIEAA
CCHHHHHHHHHHCCCHHHHHHHHHHEEECCCCEEEECCCCCCCCCCCCCCCEEEEEEHHH
IKPDDPHFAITRLLALENTLGGKLLPFGYVQQATQLAQAKGLSRHLDGARLFNAAVAQAA
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHH
LSVRAELVEAAAGTSTGSVRTEVTRDSAVAEARRIAQCFDSVSVCFSKGLGAPVGSALCG
HHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCC
SREFIARARRVRKMAGGAMRQSGMLAAAASYALDHHIERLAQDHALARQLADGLAGIEGL
CHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
HVEAPETNILFVDLSGAARDKSAALLKYLADHGIQATGLYRLRFVTHLDVDAAGVDRAVA
EEECCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHHHHHHH
AIRQFFHLKG
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Pyridoxal phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: 1.7

Km value (mM): 2.85 {L-Thr}} 0.24 {DL-erythro-phenylserine}} 0.22 {L-allo-Thr}} 0.12 {DL-threo-phenylserine}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = glycine + acetaldehyde

General reaction: Elimination of an aldehyde, C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9171400; 9228760 [H]