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The map label for this gene is nac [H]

Identifier: 91790154

GI number: 91790154

Start: 4550921

End: 4551829

Strand: Reverse

Name: nac [H]

Synonym: Bpro_4318

Alternate gene names: 91790154

Gene position: 4551829-4550921 (Counterclockwise)

Preceding gene: 91790161

Following gene: 91790153

Centisome position: 87.53

GC content: 64.58

Gene sequence:

>909_bases
ATGGACCTTCGGCAGCTGGAGTACTTTGTTCGTGTCGCCGAGCTGGGCAGCTTTACGCGGGCCGCGCTCGCACTGGATAT
TGCGCAGCCAGCGCTCAGCCGTCAGGTTCGGCTGCTCGAAGTGGAATTGCGCCAGAGTCTGCTGACCCGCAACGGCAGGG
GTGCCGTGCCTACCGATGCCGGCAGGCTTCTGCTGGAGCACGGACGCGGCATTTTGCATCAGGTCGAGCGTGCACGCGAG
GAACTGGGCCGCGTACGCGGCGCTCTCGCCGGCCGGGTTGCAATCGGCCTGCCGCCCAGTGTGGCCAAGGCGCTCACTGT
TCCGCTTCTTCGCGAAGTTCGAAAGCGCATGCCGGAAGCCGCACTGTCCGTCACGGAGGGCCTATCGGTGGCGATGCATG
AGTCGCTCATCAACGGTCGGCTAGATATCGCATTGCTGTACAACGCCGTCCAGACGCCCGACGTGGAACTTACCCCCTTG
CTCGAAGAGCAACTATTCGTGGTACAGCGCAGGACCGGCAAGGCACTGCCCGCGGCGAAGGCGAGGGCGATATCGCTGCG
CGAAGTAGCCAGCCTGCCGCTGGTGATTCCGAGCCGCCCCAATGCGATCAGGATGCTCGTGGAAACCGAGATGGCCCACC
TGGGTTGCCGGCCGCAGGTGGCTCTGGAGATCGATGGCGTTGCAGCGATTCTCGATCTGGTCGAGGACGGCGCGGGCAGT
GCCATCTTGCCACGCAATGCCGTCGCCACCGCCGCGCGACCGCAGGCTTTCGTGACACGGCCGATCAGCGCGCCCAGCCT
GCGTACCAAGCTGGTGATGGCGATGAGCTCGCGGCGCCCGGCGACACTCACGCAAAAGGCGATGCTGGAACTGATCCAGA
AAATGGCTCTGAAGCTGCTGGCCCAATGA

Upstream 100 bases:

>100_bases
TTTGCTTCGCACCGCATCTCACATGGAAGCCATCAGCCGCACTATCAGCTAGAACAATCGGTTATACCTGACCGCCCGCT
TTGTCGCTAAACTGTGATTC

Downstream 100 bases:

>100_bases
GTGCCGCCCGGCCGCCAATCTCAAGGGTTGGTACAAGCGAAGCTCGAGGAAGAGTTACCTCAATCAAGCGATGACCTGGT
GGCGCGGATCACGTCAGCCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Nitrogen assimilation control protein [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MDLRQLEYFVRVAELGSFTRAALALDIAQPALSRQVRLLEVELRQSLLTRNGRGAVPTDAGRLLLEHGRGILHQVERARE
ELGRVRGALAGRVAIGLPPSVAKALTVPLLREVRKRMPEAALSVTEGLSVAMHESLINGRLDIALLYNAVQTPDVELTPL
LEEQLFVVQRRTGKALPAAKARAISLREVASLPLVIPSRPNAIRMLVETEMAHLGCRPQVALEIDGVAAILDLVEDGAGS
AILPRNAVATAARPQAFVTRPISAPSLRTKLVMAMSSRRPATLTQKAMLELIQKMALKLLAQ

Sequences:

>Translated_302_residues
MDLRQLEYFVRVAELGSFTRAALALDIAQPALSRQVRLLEVELRQSLLTRNGRGAVPTDAGRLLLEHGRGILHQVERARE
ELGRVRGALAGRVAIGLPPSVAKALTVPLLREVRKRMPEAALSVTEGLSVAMHESLINGRLDIALLYNAVQTPDVELTPL
LEEQLFVVQRRTGKALPAAKARAISLREVASLPLVIPSRPNAIRMLVETEMAHLGCRPQVALEIDGVAAILDLVEDGAGS
AILPRNAVATAARPQAFVTRPISAPSLRTKLVMAMSSRRPATLTQKAMLELIQKMALKLLAQ
>Mature_302_residues
MDLRQLEYFVRVAELGSFTRAALALDIAQPALSRQVRLLEVELRQSLLTRNGRGAVPTDAGRLLLEHGRGILHQVERARE
ELGRVRGALAGRVAIGLPPSVAKALTVPLLREVRKRMPEAALSVTEGLSVAMHESLINGRLDIALLYNAVQTPDVELTPL
LEEQLFVVQRRTGKALPAAKARAISLREVASLPLVIPSRPNAIRMLVETEMAHLGCRPQVALEIDGVAAILDLVEDGAGS
AILPRNAVATAARPQAFVTRPISAPSLRTKLVMAMSSRRPATLTQKAMLELIQKMALKLLAQ

Specific function: Transcriptional activator for the hut, put and ure operons and repressor for the gdh and gltB operons in response to nitrogen limitation. Negative regulator of its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788297, Length=248, Percent_Identity=38.3064516129032, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI157672245, Length=247, Percent_Identity=31.5789473684211, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1787601, Length=263, Percent_Identity=27.3764258555133, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788887, Length=291, Percent_Identity=28.5223367697594, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI145693105, Length=271, Percent_Identity=25.4612546125461, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787879, Length=253, Percent_Identity=30.8300395256917, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790399, Length=268, Percent_Identity=25.7462686567164, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787589, Length=258, Percent_Identity=28.6821705426357, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788748, Length=242, Percent_Identity=26.4462809917355, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787806, Length=254, Percent_Identity=27.9527559055118, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787530, Length=302, Percent_Identity=24.1721854304636, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790208, Length=257, Percent_Identity=25.2918287937743, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788481, Length=253, Percent_Identity=26.0869565217391, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789639, Length=149, Percent_Identity=30.2013422818792, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789506, Length=265, Percent_Identity=22.6415094339623, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI2367136, Length=168, Percent_Identity=30.3571428571429, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1786401, Length=141, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32747; Mature: 32747

Theoretical pI: Translated: 11.19; Mature: 11.19

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRQLEYFVRVAELGSFTRAALALDIAQPALSRQVRLLEVELRQSLLTRNGRGAVPTDA
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
GRLLLEHGRGILHQVERAREELGRVRGALAGRVAIGLPPSVAKALTVPLLREVRKRMPEA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHCCHH
ALSVTEGLSVAMHESLINGRLDIALLYNAVQTPDVELTPLLEEQLFVVQRRTGKALPAAK
HHHHHHHHHHHHHHHHHCCCCHHHHEEHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHH
ARAISLREVASLPLVIPSRPNAIRMLVETEMAHLGCRPQVALEIDGVAAILDLVEDGAGS
HHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHCCCCC
AILPRNAVATAARPQAFVTRPISAPSLRTKLVMAMSSRRPATLTQKAMLELIQKMALKLL
EECCCCHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
AQ
CC
>Mature Secondary Structure
MDLRQLEYFVRVAELGSFTRAALALDIAQPALSRQVRLLEVELRQSLLTRNGRGAVPTDA
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
GRLLLEHGRGILHQVERAREELGRVRGALAGRVAIGLPPSVAKALTVPLLREVRKRMPEA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHCCHH
ALSVTEGLSVAMHESLINGRLDIALLYNAVQTPDVELTPLLEEQLFVVQRRTGKALPAAK
HHHHHHHHHHHHHHHHHCCCCHHHHEEHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCHH
ARAISLREVASLPLVIPSRPNAIRMLVETEMAHLGCRPQVALEIDGVAAILDLVEDGAGS
HHHHHHHHHHCCCEEECCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHCCCCC
AILPRNAVATAARPQAFVTRPISAPSLRTKLVMAMSSRRPATLTQKAMLELIQKMALKLL
EECCCCHHHHCCCCHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
AQ
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9495755; 9097040; 9278503; 8529872 [H]