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The map label for this gene is rep [H]

Identifier: 91790119

GI number: 91790119

Start: 4514211

End: 4516361

Strand: Direct

Name: rep [H]

Synonym: Bpro_4281

Alternate gene names: 91790119

Gene position: 4514211-4516361 (Clockwise)

Preceding gene: 91790117

Following gene: 91790121

Centisome position: 86.81

GC content: 63.69

Gene sequence:

>2151_bases
ATGTCGTCCCCCGCCCCTGTTTCCCCCATCTCCACTGCTGCGCCGTCTGGCGCTTTTCCCGCCAGTACCGGGCTCAACCT
CGCGCAGCAGGAAGCCGTCAACTACCTGCACGGCCCCTGCCTGGTGCTGGCCGGTGCCGGCTCGGGCAAGACGCGGGTGA
TCACGCACAAGATCGGCCGGCTGATCCAGTCCGGGCTGAAGGCCGAGCAGATTGCCGCCATCACCTTCACCAACAAGGCG
GCCGCCGAGATGCGCGAACGCGCCAAAAGCCTGATCGGCAAGCAAGCCAAGGGCGTGCTGATCTGCACCTTTCACGCACT
GGGCGTGCGCATGCTGCGGCAGGACGGCCAGGCGCTGGGCCTGAAGCAGCAGTTCTCTATCCTGGACAGCGATGACGTGA
CCAGCATTTTGAAAGACGCCGGCGGCAGCACCGACGTGGCAACGGCGCGCCAGTGGCAGTGGACCATCAGCGCCTGGAAA
GGCCAGGGCCTCAATGCCGCCCAAGCCGCAGCCCAGGCCAGCAACGACGAAGAGCGCCTGGTGGCGCGCGTCATGGCGCA
CTATGAAGAGCGCCTGACGGCCTACCAGAGCGTGGACTTTGACGACCTGATCGGCCTGCCGCTCAAGCTGCTGCGGGAGC
ACGAAGCGGTCCGCAACAAATGGCAGCAGTCGCTGGGCCATGTGCTGGTCGACGAATACCAGGACACCAACGCCACGCAA
TATGAGGTGCTCAAACTGCTGGTGGGCACGCGCGGCCGCTTCACGGCGGTGGGCGATGACGACCAGTCGATCTACGGCTG
GCGCGGCGCCACGCTGGACAACCTGAAGAAACTGCCGGAGGACTACCCCAACCTCAAGGTGGTGAAGCTGGAGCAGAACT
ACCGCTCGACCAGCGCCATCCTGCGCGCCGCCAACAACGTGATCGGCCCCAATCCCAAGCTGTTTCCAAAGACCCTGTTC
TCGGAACTCGGCGAGGGCGAGCCGGTGCGCGTGGTGGACTGTGACAGCGAAGAGCACGAGGCCGAGCGCGTGGTGGCCCG
CATCCAGAGCCTGCGCGCGGGCGGCGAGCTGCAGGCGGTGGGCAGCCAGTACAAGGAATGGAAAGATTTTTGCGTGCTCT
ACCGCGCCAATCACCAGGCCAAGCCGCTGGAAAAAGCCTTTCGCAAGGCGCAGATCCCGTACAAGGTGTCGGGCGGGCAA
AGCTTTTTCGACCGCGCCGAGATCAAGGACCTGTGCAGCTGGATGGTCCTGCTGGTCAACAACGATAACAACCCGGCCTT
CATGCGCTCGGTGACCACGCCCAAGCGCGGCATCGGCCACACCACGCTGGGCACCTTGGGCACCTTTGCCGACCAGTACA
AGCTCAGCCTGTTTGAAGCGCTGTTCAGCAACTCATTGAGCTCGGTGCTGCCGGCCAAGGCGGTCGGTTCGCTGCAGGAA
TTCGGCCGCTACCTGAACGAGCTGGAATACCGTGCCAGGCGCACCGTGGGCGCGGAAGATGCGCGCACGTTTCTGAACGA
CTGGCTCAAGGAAATCGACTACGAAAAACACCTCTACGACGGCGAAGACAGTGAAAAAGTCGCGGCCGCGCGCTGGAGCA
ATGTGATGGATTTTTGCGACTGGATGGCGCTGCGCTGCGGCGGCCAGATCGACGACACCTCGGGCGCCGTGGTTGAAAGC
GAAAAGAAAACCATGCTCGAAGTGGTGCAGACGATTGCGCTGCTGTCCACCATCAGCGAGCGCGAGGGTGACCAGAATGT
GGTGACACTGTCCACCCTGCACGCCTCCAAGGGCCTGGAGTGGCCGCATGTGATGCTGGCCGGCTGCAACGAAGGCCTGC
TGCCCTTCAAGCTCGAAGAAGACGACGGCAGCAACACCGACAACATTGCGTTGCGCCTGCAGGAAGAGCGCCGCCTGATG
TATGTGGGCATCACCCGCGCCCAGCGCACGCTGGCGGTCAACTGGCTGCGCCGGCGCAAGAAGAACCGTGACACCATCGC
CGGCATCCCGAGCCGGTTTATTGCCGAGATGGCGCTGGACAAGGCCACGGTGAAAGAAGACCCGCGCGAAAAGCTCAAGG
CCCTGCGGGCCGAGTTTGCCAAAAAATCAGCCGATGGACTGGCCGCCGCGGCGGCCTCGGAACTACGTTAG

Upstream 100 bases:

>100_bases
TTTTTCGGTCCCCTGATCCAGATTACCGATTACCGATTCCTTCACGCCAATCCCCGCTTACCCCACGCCGCTTCCCATTT
TCTCCACGCGTCCTGCCTGC

Downstream 100 bases:

>100_bases
TGCAGTGTTGCAGTGCGTAACTCAGGCCCGTGCCGTCAAATGCTCAGCCACTTGCCCAAGCCGATCAGGGCGGCCTGGTC
GGATACCAGAAAGCCCTGGA

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 716; Mature: 715

Protein sequence:

>716_residues
MSSPAPVSPISTAAPSGAFPASTGLNLAQQEAVNYLHGPCLVLAGAGSGKTRVITHKIGRLIQSGLKAEQIAAITFTNKA
AAEMRERAKSLIGKQAKGVLICTFHALGVRMLRQDGQALGLKQQFSILDSDDVTSILKDAGGSTDVATARQWQWTISAWK
GQGLNAAQAAAQASNDEERLVARVMAHYEERLTAYQSVDFDDLIGLPLKLLREHEAVRNKWQQSLGHVLVDEYQDTNATQ
YEVLKLLVGTRGRFTAVGDDDQSIYGWRGATLDNLKKLPEDYPNLKVVKLEQNYRSTSAILRAANNVIGPNPKLFPKTLF
SELGEGEPVRVVDCDSEEHEAERVVARIQSLRAGGELQAVGSQYKEWKDFCVLYRANHQAKPLEKAFRKAQIPYKVSGGQ
SFFDRAEIKDLCSWMVLLVNNDNNPAFMRSVTTPKRGIGHTTLGTLGTFADQYKLSLFEALFSNSLSSVLPAKAVGSLQE
FGRYLNELEYRARRTVGAEDARTFLNDWLKEIDYEKHLYDGEDSEKVAAARWSNVMDFCDWMALRCGGQIDDTSGAVVES
EKKTMLEVVQTIALLSTISEREGDQNVVTLSTLHASKGLEWPHVMLAGCNEGLLPFKLEEDDGSNTDNIALRLQEERRLM
YVGITRAQRTLAVNWLRRRKKNRDTIAGIPSRFIAEMALDKATVKEDPREKLKALRAEFAKKSADGLAAAAASELR

Sequences:

>Translated_716_residues
MSSPAPVSPISTAAPSGAFPASTGLNLAQQEAVNYLHGPCLVLAGAGSGKTRVITHKIGRLIQSGLKAEQIAAITFTNKA
AAEMRERAKSLIGKQAKGVLICTFHALGVRMLRQDGQALGLKQQFSILDSDDVTSILKDAGGSTDVATARQWQWTISAWK
GQGLNAAQAAAQASNDEERLVARVMAHYEERLTAYQSVDFDDLIGLPLKLLREHEAVRNKWQQSLGHVLVDEYQDTNATQ
YEVLKLLVGTRGRFTAVGDDDQSIYGWRGATLDNLKKLPEDYPNLKVVKLEQNYRSTSAILRAANNVIGPNPKLFPKTLF
SELGEGEPVRVVDCDSEEHEAERVVARIQSLRAGGELQAVGSQYKEWKDFCVLYRANHQAKPLEKAFRKAQIPYKVSGGQ
SFFDRAEIKDLCSWMVLLVNNDNNPAFMRSVTTPKRGIGHTTLGTLGTFADQYKLSLFEALFSNSLSSVLPAKAVGSLQE
FGRYLNELEYRARRTVGAEDARTFLNDWLKEIDYEKHLYDGEDSEKVAAARWSNVMDFCDWMALRCGGQIDDTSGAVVES
EKKTMLEVVQTIALLSTISEREGDQNVVTLSTLHASKGLEWPHVMLAGCNEGLLPFKLEEDDGSNTDNIALRLQEERRLM
YVGITRAQRTLAVNWLRRRKKNRDTIAGIPSRFIAEMALDKATVKEDPREKLKALRAEFAKKSADGLAAAAASELR
>Mature_715_residues
SSPAPVSPISTAAPSGAFPASTGLNLAQQEAVNYLHGPCLVLAGAGSGKTRVITHKIGRLIQSGLKAEQIAAITFTNKAA
AEMRERAKSLIGKQAKGVLICTFHALGVRMLRQDGQALGLKQQFSILDSDDVTSILKDAGGSTDVATARQWQWTISAWKG
QGLNAAQAAAQASNDEERLVARVMAHYEERLTAYQSVDFDDLIGLPLKLLREHEAVRNKWQQSLGHVLVDEYQDTNATQY
EVLKLLVGTRGRFTAVGDDDQSIYGWRGATLDNLKKLPEDYPNLKVVKLEQNYRSTSAILRAANNVIGPNPKLFPKTLFS
ELGEGEPVRVVDCDSEEHEAERVVARIQSLRAGGELQAVGSQYKEWKDFCVLYRANHQAKPLEKAFRKAQIPYKVSGGQS
FFDRAEIKDLCSWMVLLVNNDNNPAFMRSVTTPKRGIGHTTLGTLGTFADQYKLSLFEALFSNSLSSVLPAKAVGSLQEF
GRYLNELEYRARRTVGAEDARTFLNDWLKEIDYEKHLYDGEDSEKVAAARWSNVMDFCDWMALRCGGQIDDTSGAVVESE
KKTMLEVVQTIALLSTISEREGDQNVVTLSTLHASKGLEWPHVMLAGCNEGLLPFKLEEDDGSNTDNIALRLQEERRLMY
VGITRAQRTLAVNWLRRRKKNRDTIAGIPSRFIAEMALDKATVKEDPREKLKALRAEFAKKSADGLAAAAASELR

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=667, Percent_Identity=45.5772113943029, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI2367296, Length=669, Percent_Identity=35.4260089686099, Blast_Score=352, Evalue=4e-98,
Organism=Saccharomyces cerevisiae, GI6322369, Length=766, Percent_Identity=26.5013054830287, Blast_Score=183, Evalue=9e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 79152; Mature: 79020

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPAPVSPISTAAPSGAFPASTGLNLAQQEAVNYLHGPCLVLAGAGSGKTRVITHKIGR
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
LIQSGLKAEQIAAITFTNKAAAEMRERAKSLIGKQAKGVLICTFHALGVRMLRQDGQALG
HHHHCCCHHHEEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCHHCC
LKQQFSILDSDDVTSILKDAGGSTDVATARQWQWTISAWKGQGLNAAQAAAQASNDEERL
CHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHEEEEEEHHCCCCCCHHHHHHHCCCHHHHH
VARVMAHYEERLTAYQSVDFDDLIGLPLKLLREHEAVRNKWQQSLGHVLVDEYQDTNATQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
YEVLKLLVGTRGRFTAVGDDDQSIYGWRGATLDNLKKLPEDYPNLKVVKLEQNYRSTSAI
HHHHHHHHCCCCCEEEECCCCCCEECCCCCCHHHHHHCHHCCCCCEEEEECCCCHHHHHH
LRAANNVIGPNPKLFPKTLFSELGEGEPVRVVDCDSEEHEAERVVARIQSLRAGGELQAV
HHHHHCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHH
GSQYKEWKDFCVLYRANHQAKPLEKAFRKAQIPYKVSGGQSFFDRAEIKDLCSWMVLLVN
HHHHHHHHHHEEHEECCCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHEEEEEE
NDNNPAFMRSVTTPKRGIGHTTLGTLGTFADQYKLSLFEALFSNSLSSVLPAKAVGSLQE
CCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHH
FGRYLNELEYRARRTVGAEDARTFLNDWLKEIDYEKHLYDGEDSEKVAAARWSNVMDFCD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHH
WMALRCGGQIDDTSGAVVESEKKTMLEVVQTIALLSTISEREGDQNVVTLSTLHASKGLE
HHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHCCCC
WPHVMLAGCNEGLLPFKLEEDDGSNTDNIALRLQEERRLMYVGITRAQRTLAVNWLRRRK
CCHHEEECCCCCCCCEEECCCCCCCCCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHH
KNRDTIAGIPSRFIAEMALDKATVKEDPREKLKALRAEFAKKSADGLAAAAASELR
CCCCCHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCC
>Mature Secondary Structure 
SSPAPVSPISTAAPSGAFPASTGLNLAQQEAVNYLHGPCLVLAGAGSGKTRVITHKIGR
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHH
LIQSGLKAEQIAAITFTNKAAAEMRERAKSLIGKQAKGVLICTFHALGVRMLRQDGQALG
HHHHCCCHHHEEEEEECCHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHCCHHCC
LKQQFSILDSDDVTSILKDAGGSTDVATARQWQWTISAWKGQGLNAAQAAAQASNDEERL
CHHHHHCCCCHHHHHHHHHCCCCCCCCCCHHEEEEEEHHCCCCCCHHHHHHHCCCHHHHH
VARVMAHYEERLTAYQSVDFDDLIGLPLKLLREHEAVRNKWQQSLGHVLVDEYQDTNATQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
YEVLKLLVGTRGRFTAVGDDDQSIYGWRGATLDNLKKLPEDYPNLKVVKLEQNYRSTSAI
HHHHHHHHCCCCCEEEECCCCCCEECCCCCCHHHHHHCHHCCCCCEEEEECCCCHHHHHH
LRAANNVIGPNPKLFPKTLFSELGEGEPVRVVDCDSEEHEAERVVARIQSLRAGGELQAV
HHHHHCCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHH
GSQYKEWKDFCVLYRANHQAKPLEKAFRKAQIPYKVSGGQSFFDRAEIKDLCSWMVLLVN
HHHHHHHHHHEEHEECCCCCCHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHEEEEEE
NDNNPAFMRSVTTPKRGIGHTTLGTLGTFADQYKLSLFEALFSNSLSSVLPAKAVGSLQE
CCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHH
FGRYLNELEYRARRTVGAEDARTFLNDWLKEIDYEKHLYDGEDSEKVAAARWSNVMDFCD
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHH
WMALRCGGQIDDTSGAVVESEKKTMLEVVQTIALLSTISEREGDQNVVTLSTLHASKGLE
HHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEHHHCCCC
WPHVMLAGCNEGLLPFKLEEDDGSNTDNIALRLQEERRLMYVGITRAQRTLAVNWLRRRK
CCHHEEECCCCCCCCEEECCCCCCCCCCEEEEEECCCEEEEEEEHHHHHHHHHHHHHHHH
KNRDTIAGIPSRFIAEMALDKATVKEDPREKLKALRAEFAKKSADGLAAAAASELR
CCCCCHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]