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The map label for this gene is priA [H]

Identifier: 91790103

GI number: 91790103

Start: 4496889

End: 4499108

Strand: Direct

Name: priA [H]

Synonym: Bpro_4265

Alternate gene names: 91790103

Gene position: 4496889-4499108 (Clockwise)

Preceding gene: 91790102

Following gene: 91790106

Centisome position: 86.47

GC content: 67.52

Gene sequence:

>2220_bases
ATGACTTACTGGATCGAGGTGGTCGTGGCCACGCCGGCCCACAGTGCTGTCGCCGGACCGCTGACTTATCGAAGTGAGTT
CCCACTGTTCCCGGGCGCGCTGGTGCGTGTTCCCCTGGGCAAGCGGGAAGTGCTGGGCGTGGTGTGGAACCTGCTGCCGG
ACAGCGGTGGCCTGCCGGAAAAGCAGATCCGTTCCATTGCCGGCGCCCTCGATGGGCTGGCCCCCCTGGCCACGACCTGG
CGAAAACTGGTCGCATTCACGGCTGGCTACTACCAGCGCTCGCTGGGCGAAGTGGCGTTGGCCGCCCTGCCGCCGCAACT
GCGCGAACTCAGCGCCGTGCAGCTGGCGCGCCGGCTCAAGCGCCCCGTGCTGGACACCAGTTGCGCCCCGGGCACTGCTG
ATTTAATAGCACTCACCCCACAACAGACGAAAGCACTGGCCGAATTTGATGGCGATGCTGATGCCCGGCCGGCCCTGCTG
TTTGGCGCCACCGGCAGCGGCAAAACCGAAGTCTACCTGCGGGCGGCGGCCCGCGTGCTGGAGCAGGACCCGGACGCCCA
GGTGCTGGTCATGGTGCCCGAAATCAACCTCACGCCGCAGTTGCAGGCCCGGTTTGAGGCGCGCTTTGCCCACCTGGGCA
AGGAGCGGGTCGTGCCCATGCACAGCGGGCTCACGCCGGCCCAGCGACTCAAAGGCTGGCTGGCGGCCCACACAGGGCAG
GCCCGGCTGGTGCTGGGCACCCGCATGGCGGTGCTGGCCTCTCTGCCCAACTTGCGCCTGCTGGTCGTCGATGAAGAGCA
TGACCCGAGCTACAAGCAGCAGGAAGGTGCGCGCTACTCGGCACGCGACCTGGCGGTTTACCGCGCCAAGCTCGAAACCG
CCGAAGCCAGACATGCGCACGGCCCCTGCCGCGTGCTGCTGGGTTCGGCCACGCCCTCGCTGGAAAGCTGGCAGGCCACC
CTGACCGGCCGCTACCAGCGCCTGACCATGACCGAACGCATTGGTGTTCCATCATCGATAGGCATGGAAGCGCTGCAATT
TTCCGACGGGCCGCCCCTAGGAAAATTAGCCCCCCCGGGGGGCAGCGAGCTACGCGCAGCGAGCGAGCGTGGGGGCCTAC
CGTTAGTGCGCCGGGTGGACATGAACCACCAGCCCAAACACTGCATCATTGCCCCGCCCCTGGTTGAAGCGATTGCCCAG
CGGGTGGCGCGCGGCGAGCAATCGCTGATTTTCCTGAACCGGCGCGGCTATGCGCCGGTACTGGCCTGCCACGATTGCGG
CTGGAAAAGCGAATGCCCGCACTGCAGTGCCTACCGGGTGTTTCACAAGATAGACCGCACCCTGCGCTGCCACCACTGCG
GTTTCACGGAGCGGGTGCCGCGAGCCTGCCCGGACTGCGGCAACATCGACATCGCGCCCGTGGGGCGCGGCACCGAACGG
CTGGAAGAGCACCTCGCCGAGCTGCTGGCCCATGTGCAGCGGCCCGATGACGGCGGAGTGCCGGGGCCGGTCAGGATTGC
CCGCATCGATGCCGACAGCACGCGGCTCAAGGGCGCCCTCGAATCGCAACTGGCCAGCGTCCATGCCCGCGAGGTCGATG
TCCTGGTCGGCACGCAAATGATTGCCAAGGGCCATGACTTCCGGCACATCACGCTGGTGGCGGCCATCAACCCCGACGGC
GCGCTGTTCTCCAGCGACTTCCGTGCGCCCGAACGCCTGTTCAGCCTGTTGATGCAGGCCGGCGGCCGGGCCGGCCGCGA
TGCCCGGCACAGCGAGGCCAGCGAGATGTGGGTGCAGACTTTCCACCCGGACCATCCGCTGTTCGAAGCGCTGAAAAAGC
ACGACTACCCGGCCTTTGCCGCCCGGCAGCTGGCCGAGCGGGAAGCCGCCGGCATGGCGCCCTTCGGCTTTTCAGCGCTG
GTGCGCGCCGAGGCCCGCGCGCAGGAGGTGGCGCAGGGCTTCCTCAACGCCGCCGCCGCATCGGCAAAAGCGCAGCAGGT
CACCGGCCACGACCAGGTGGCGGCCTACCCGGCCGTACCGATGACGATCCAGCGCGTGGCCAACATCGAGCGCGCACAAA
TGCTGGTGGAAAGCCCGTCACGCGCAGCCCTGCAGCGCTTCCTGGCCGCGTGGCAGCCGGTGCTGTTTGAAACCCGCCAG
CACAGCGATTTCAAAAGCCTGATCCGCTGGGCGGTGGATGTGGACCCGATTGCGATTTAA

Upstream 100 bases:

>100_bases
CCACAGAAAATCTGGAAATCTGGAAAGCAGTGGTAAATCAAGCACTTAGCGCGTTAACTGGAAGTGCTTTTGAAGACATC
CTCGCATCTGGTTGTCCCGC

Downstream 100 bases:

>100_bases
GGGCCGGTTACCGGCCAGGAGCCACCGGTCGAATGTCCATGGCGACTGAAGACTGAACGCAGTCCGTCACCCTTGCGCAA
AGCCGAGCTTCAACGTTCAA

Product: primosomal protein N'

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 739; Mature: 738

Protein sequence:

>739_residues
MTYWIEVVVATPAHSAVAGPLTYRSEFPLFPGALVRVPLGKREVLGVVWNLLPDSGGLPEKQIRSIAGALDGLAPLATTW
RKLVAFTAGYYQRSLGEVALAALPPQLRELSAVQLARRLKRPVLDTSCAPGTADLIALTPQQTKALAEFDGDADARPALL
FGATGSGKTEVYLRAAARVLEQDPDAQVLVMVPEINLTPQLQARFEARFAHLGKERVVPMHSGLTPAQRLKGWLAAHTGQ
ARLVLGTRMAVLASLPNLRLLVVDEEHDPSYKQQEGARYSARDLAVYRAKLETAEARHAHGPCRVLLGSATPSLESWQAT
LTGRYQRLTMTERIGVPSSIGMEALQFSDGPPLGKLAPPGGSELRAASERGGLPLVRRVDMNHQPKHCIIAPPLVEAIAQ
RVARGEQSLIFLNRRGYAPVLACHDCGWKSECPHCSAYRVFHKIDRTLRCHHCGFTERVPRACPDCGNIDIAPVGRGTER
LEEHLAELLAHVQRPDDGGVPGPVRIARIDADSTRLKGALESQLASVHAREVDVLVGTQMIAKGHDFRHITLVAAINPDG
ALFSSDFRAPERLFSLLMQAGGRAGRDARHSEASEMWVQTFHPDHPLFEALKKHDYPAFAARQLAEREAAGMAPFGFSAL
VRAEARAQEVAQGFLNAAAASAKAQQVTGHDQVAAYPAVPMTIQRVANIERAQMLVESPSRAALQRFLAAWQPVLFETRQ
HSDFKSLIRWAVDVDPIAI

Sequences:

>Translated_739_residues
MTYWIEVVVATPAHSAVAGPLTYRSEFPLFPGALVRVPLGKREVLGVVWNLLPDSGGLPEKQIRSIAGALDGLAPLATTW
RKLVAFTAGYYQRSLGEVALAALPPQLRELSAVQLARRLKRPVLDTSCAPGTADLIALTPQQTKALAEFDGDADARPALL
FGATGSGKTEVYLRAAARVLEQDPDAQVLVMVPEINLTPQLQARFEARFAHLGKERVVPMHSGLTPAQRLKGWLAAHTGQ
ARLVLGTRMAVLASLPNLRLLVVDEEHDPSYKQQEGARYSARDLAVYRAKLETAEARHAHGPCRVLLGSATPSLESWQAT
LTGRYQRLTMTERIGVPSSIGMEALQFSDGPPLGKLAPPGGSELRAASERGGLPLVRRVDMNHQPKHCIIAPPLVEAIAQ
RVARGEQSLIFLNRRGYAPVLACHDCGWKSECPHCSAYRVFHKIDRTLRCHHCGFTERVPRACPDCGNIDIAPVGRGTER
LEEHLAELLAHVQRPDDGGVPGPVRIARIDADSTRLKGALESQLASVHAREVDVLVGTQMIAKGHDFRHITLVAAINPDG
ALFSSDFRAPERLFSLLMQAGGRAGRDARHSEASEMWVQTFHPDHPLFEALKKHDYPAFAARQLAEREAAGMAPFGFSAL
VRAEARAQEVAQGFLNAAAASAKAQQVTGHDQVAAYPAVPMTIQRVANIERAQMLVESPSRAALQRFLAAWQPVLFETRQ
HSDFKSLIRWAVDVDPIAI
>Mature_738_residues
TYWIEVVVATPAHSAVAGPLTYRSEFPLFPGALVRVPLGKREVLGVVWNLLPDSGGLPEKQIRSIAGALDGLAPLATTWR
KLVAFTAGYYQRSLGEVALAALPPQLRELSAVQLARRLKRPVLDTSCAPGTADLIALTPQQTKALAEFDGDADARPALLF
GATGSGKTEVYLRAAARVLEQDPDAQVLVMVPEINLTPQLQARFEARFAHLGKERVVPMHSGLTPAQRLKGWLAAHTGQA
RLVLGTRMAVLASLPNLRLLVVDEEHDPSYKQQEGARYSARDLAVYRAKLETAEARHAHGPCRVLLGSATPSLESWQATL
TGRYQRLTMTERIGVPSSIGMEALQFSDGPPLGKLAPPGGSELRAASERGGLPLVRRVDMNHQPKHCIIAPPLVEAIAQR
VARGEQSLIFLNRRGYAPVLACHDCGWKSECPHCSAYRVFHKIDRTLRCHHCGFTERVPRACPDCGNIDIAPVGRGTERL
EEHLAELLAHVQRPDDGGVPGPVRIARIDADSTRLKGALESQLASVHAREVDVLVGTQMIAKGHDFRHITLVAAINPDGA
LFSSDFRAPERLFSLLMQAGGRAGRDARHSEASEMWVQTFHPDHPLFEALKKHDYPAFAARQLAEREAAGMAPFGFSALV
RAEARAQEVAQGFLNAAAASAKAQQVTGHDQVAAYPAVPMTIQRVANIERAQMLVESPSRAALQRFLAAWQPVLFETRQH
SDFKSLIRWAVDVDPIAI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=612, Percent_Identity=40.359477124183, Blast_Score=389, Evalue=1e-109,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 80482; Mature: 80351

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYWIEVVVATPAHSAVAGPLTYRSEFPLFPGALVRVPLGKREVLGVVWNLLPDSGGLPE
CCEEEEEEEECCCCHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCH
KQIRSIAGALDGLAPLATTWRKLVAFTAGYYQRSLGEVALAALPPQLRELSAVQLARRLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RPVLDTSCAPGTADLIALTPQQTKALAEFDGDADARPALLFGATGSGKTEVYLRAAARVL
CCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
EQDPDAQVLVMVPEINLTPQLQARFEARFAHLGKERVVPMHSGLTPAQRLKGWLAAHTGQ
HCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCC
ARLVLGTRMAVLASLPNLRLLVVDEEHDPSYKQQEGARYSARDLAVYRAKLETAEARHAH
EEEEHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
GPCRVLLGSATPSLESWQATLTGRYQRLTMTERIGVPSSIGMEALQFSDGPPLGKLAPPG
CCEEEEECCCCCCHHHHHHHHCCCHHEEEHHHHCCCCHHHCCHHHCCCCCCCCCCCCCCC
GSELRAASERGGLPLVRRVDMNHQPKHCIIAPPLVEAIAQRVARGEQSLIFLNRRGYAPV
CHHHHHHHHCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCE
LACHDCGWKSECPHCSAYRVFHKIDRTLRCHHCGFTERVPRACPDCGNIDIAPVGRGTER
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEECCCCHHH
LEEHLAELLAHVQRPDDGGVPGPVRIARIDADSTRLKGALESQLASVHAREVDVLVGTQM
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAKGHDFRHITLVAAINPDGALFSSDFRAPERLFSLLMQAGGRAGRDARHSEASEMWVQT
HHCCCCCEEEEEEEEECCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
FHPDHPLFEALKKHDYPAFAARQLAEREAAGMAPFGFSALVRAEARAQEVAQGFLNAAAA
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SAKAQQVTGHDQVAAYPAVPMTIQRVANIERAQMLVESPSRAALQRFLAAWQPVLFETRQ
HHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
HSDFKSLIRWAVDVDPIAI
CCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TYWIEVVVATPAHSAVAGPLTYRSEFPLFPGALVRVPLGKREVLGVVWNLLPDSGGLPE
CEEEEEEEECCCCHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCCH
KQIRSIAGALDGLAPLATTWRKLVAFTAGYYQRSLGEVALAALPPQLRELSAVQLARRLK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
RPVLDTSCAPGTADLIALTPQQTKALAEFDGDADARPALLFGATGSGKTEVYLRAAARVL
CCCCCCCCCCCCCCEEEECCHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
EQDPDAQVLVMVPEINLTPQLQARFEARFAHLGKERVVPMHSGLTPAQRLKGWLAAHTGQ
HCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHCCCC
ARLVLGTRMAVLASLPNLRLLVVDEEHDPSYKQQEGARYSARDLAVYRAKLETAEARHAH
EEEEHHHHHHHHHCCCCCEEEEEECCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
GPCRVLLGSATPSLESWQATLTGRYQRLTMTERIGVPSSIGMEALQFSDGPPLGKLAPPG
CCEEEEECCCCCCHHHHHHHHCCCHHEEEHHHHCCCCHHHCCHHHCCCCCCCCCCCCCCC
GSELRAASERGGLPLVRRVDMNHQPKHCIIAPPLVEAIAQRVARGEQSLIFLNRRGYAPV
CHHHHHHHHCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCE
LACHDCGWKSECPHCSAYRVFHKIDRTLRCHHCGFTERVPRACPDCGNIDIAPVGRGTER
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEECCCCHHH
LEEHLAELLAHVQRPDDGGVPGPVRIARIDADSTRLKGALESQLASVHAREVDVLVGTQM
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IAKGHDFRHITLVAAINPDGALFSSDFRAPERLFSLLMQAGGRAGRDARHSEASEMWVQT
HHCCCCCEEEEEEEEECCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
FHPDHPLFEALKKHDYPAFAARQLAEREAAGMAPFGFSALVRAEARAQEVAQGFLNAAAA
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SAKAQQVTGHDQVAAYPAVPMTIQRVANIERAQMLVESPSRAALQRFLAAWQPVLFETRQ
HHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
HSDFKSLIRWAVDVDPIAI
CCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]