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The map label for this gene is yafC [H]

Identifier: 91790042

GI number: 91790042

Start: 4422701

End: 4423669

Strand: Reverse

Name: yafC [H]

Synonym: Bpro_4204

Alternate gene names: 91790042

Gene position: 4423669-4422701 (Counterclockwise)

Preceding gene: 91790044

Following gene: 91790041

Centisome position: 85.07

GC content: 66.77

Gene sequence:

>969_bases
GTGGACCGCTTGCGTGCTTTCGAGGTGTTCGCCTCCGTGGTGAGCCGGGGCAGCTTCACGCGGGCAGCCGACGCCCTCGA
CACCTCCCCGGCCAATGTCACGCGCTACATCAATGAGCTGGAAGCCCACTTGGGCACGCGGCTTCTCAATCGCAGCTCGC
GCAAGCTGTCCCTGACGGAGAGTGGTGAGGCGCTGTATGAGCGCAGCAAATCCATCCTGGAAGAAGTCGCCGAGGCCGAG
GCGCTGGCCTCGTCGGCCACCCTGCAGCCGCGCGGGCGCTTGCGCATCAATGCGCCGGTGAGCTTTGGCATCCGGCATCT
GGCGCCGCTGTGGCCGCTATTCATGCAGGCCTATCCCGAGGTGGAACTGGACATTGCGCTGAGCGACCGCGTGGTGGACA
TTGTGGAAGAGGGCTACGACCTGGCCATCCGCATCTCACGCGCCGGGTCGGCCTCCCATGCGGCGCGCAAGCTGGGCACT
TCCCGCAACATCGTCTGCGCCTCGCCGGCCTACCTGAAAGCGCACGGGCAGCCCGAAAAGCCCGCAGACCTGGTGGGGCA
CCGGTGCCTCGGCTACACCTATGCGGCCACCGCCAACGAATGGCATTTTGTGGATGACGCGGGTCGGCCGCACATGGTCA
AGGTGAATTACGTCATGCATGCCAATAACGGCGACACGGCGCGCGAAGCGGCGATTGCCGGGCAGGGCGTCATCTGGCAG
CCGGCCTTCCTGATCGGGGACGATTTGAAAGCCGGGCGGCTGGTTGCCCTGATGCCCGGCTACCGCATGCCCGACATCGA
CGTGCTGGCCGTCTACCCCAGCCGCCGCCACCTGAGCGCCAAGGTCCGGGTCATGGTGGACTTTCTGGTGGAGGCCTTCC
AGCCCGTCCCTTCGTGGGAAGTCCCGCCGGGGCCGGGCGTGACGGAGAAGGCGATCGCCCGGGCGGCCCGTCATCGCCGC
GTAAAATGA

Upstream 100 bases:

>100_bases
TGTGCCCGCCGATGGATGAACTGTAGGATTTACCTTTGATTGGAAGAAGACCTGAAAAGTGGTTTAATCATTCAGTTTTT
ATGAATTTAAGGGGCTGGTG

Downstream 100 bases:

>100_bases
ACGGTTTAGCGATGGCCATCCGAGGCGTCGCTACCCGCGGCGCCGAACCAGGCGCCGCGCTTTTGTCACCAACACTCCTA
TGCTCACTTTCCAGCAAATT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MDRLRAFEVFASVVSRGSFTRAADALDTSPANVTRYINELEAHLGTRLLNRSSRKLSLTESGEALYERSKSILEEVAEAE
ALASSATLQPRGRLRINAPVSFGIRHLAPLWPLFMQAYPEVELDIALSDRVVDIVEEGYDLAIRISRAGSASHAARKLGT
SRNIVCASPAYLKAHGQPEKPADLVGHRCLGYTYAATANEWHFVDDAGRPHMVKVNYVMHANNGDTAREAAIAGQGVIWQ
PAFLIGDDLKAGRLVALMPGYRMPDIDVLAVYPSRRHLSAKVRVMVDFLVEAFQPVPSWEVPPGPGVTEKAIARAARHRR
VK

Sequences:

>Translated_322_residues
MDRLRAFEVFASVVSRGSFTRAADALDTSPANVTRYINELEAHLGTRLLNRSSRKLSLTESGEALYERSKSILEEVAEAE
ALASSATLQPRGRLRINAPVSFGIRHLAPLWPLFMQAYPEVELDIALSDRVVDIVEEGYDLAIRISRAGSASHAARKLGT
SRNIVCASPAYLKAHGQPEKPADLVGHRCLGYTYAATANEWHFVDDAGRPHMVKVNYVMHANNGDTAREAAIAGQGVIWQ
PAFLIGDDLKAGRLVALMPGYRMPDIDVLAVYPSRRHLSAKVRVMVDFLVEAFQPVPSWEVPPGPGVTEKAIARAARHRR
VK
>Mature_322_residues
MDRLRAFEVFASVVSRGSFTRAADALDTSPANVTRYINELEAHLGTRLLNRSSRKLSLTESGEALYERSKSILEEVAEAE
ALASSATLQPRGRLRINAPVSFGIRHLAPLWPLFMQAYPEVELDIALSDRVVDIVEEGYDLAIRISRAGSASHAARKLGT
SRNIVCASPAYLKAHGQPEKPADLVGHRCLGYTYAATANEWHFVDDAGRPHMVKVNYVMHANNGDTAREAAIAGQGVIWQ
PAFLIGDDLKAGRLVALMPGYRMPDIDVLAVYPSRRHLSAKVRVMVDFLVEAFQPVPSWEVPPGPGVTEKAIARAARHRR
VK

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=294, Percent_Identity=37.7551020408163, Blast_Score=197, Evalue=8e-52,
Organism=Escherichia coli, GI145693193, Length=290, Percent_Identity=33.7931034482759, Blast_Score=172, Evalue=3e-44,
Organism=Escherichia coli, GI87081978, Length=295, Percent_Identity=35.5932203389831, Blast_Score=167, Evalue=7e-43,
Organism=Escherichia coli, GI1789440, Length=285, Percent_Identity=32.6315789473684, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=31.0810810810811, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787128, Length=285, Percent_Identity=28.7719298245614, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787589, Length=290, Percent_Identity=25.8620689655172, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789173, Length=257, Percent_Identity=30.7392996108949, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786448, Length=292, Percent_Identity=24.6575342465753, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790011, Length=307, Percent_Identity=25.0814332247557, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1788481, Length=290, Percent_Identity=25.1724137931034, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=26.9360269360269, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI157672245, Length=121, Percent_Identity=33.0578512396694, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1789492, Length=280, Percent_Identity=25.3571428571429, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790399, Length=120, Percent_Identity=30, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35370; Mature: 35370

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRLRAFEVFASVVSRGSFTRAADALDTSPANVTRYINELEAHLGTRLLNRSSRKLSLTE
CCHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
SGEALYERSKSILEEVAEAEALASSATLQPRGRLRINAPVSFGIRHLAPLWPLFMQAYPE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCC
VELDIALSDRVVDIVEEGYDLAIRISRAGSASHAARKLGTSRNIVCASPAYLKAHGQPEK
EEEEEEECHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCCEEECCCHHEECCCCCCC
PADLVGHRCLGYTYAATANEWHFVDDAGRPHMVKVNYVMHANNGDTAREAAIAGQGVIWQ
CHHHHCCHHHCEEEECCCCCEEEECCCCCCEEEEEEEEEECCCCCHHHHHHHCCCCEEEC
PAFLIGDDLKAGRLVALMPGYRMPDIDVLAVYPSRRHLSAKVRVMVDFLVEAFQPVPSWE
CCEEECCCCCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCC
VPPGPGVTEKAIARAARHRRVK
CCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MDRLRAFEVFASVVSRGSFTRAADALDTSPANVTRYINELEAHLGTRLLNRSSRKLSLTE
CCHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECC
SGEALYERSKSILEEVAEAEALASSATLQPRGRLRINAPVSFGIRHLAPLWPLFMQAYPE
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCC
VELDIALSDRVVDIVEEGYDLAIRISRAGSASHAARKLGTSRNIVCASPAYLKAHGQPEK
EEEEEEECHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCCEEECCCHHEECCCCCCC
PADLVGHRCLGYTYAATANEWHFVDDAGRPHMVKVNYVMHANNGDTAREAAIAGQGVIWQ
CHHHHCCHHHCEEEECCCCCEEEECCCCCCEEEEEEEEEECCCCCHHHHHHHCCCCEEEC
PAFLIGDDLKAGRLVALMPGYRMPDIDVLAVYPSRRHLSAKVRVMVDFLVEAFQPVPSWE
CCEEECCCCCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCC
VPPGPGVTEKAIARAARHRRVK
CCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]