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The map label for this gene is dhbE [H]

Identifier: 91789964

GI number: 91789964

Start: 4329878

End: 4331548

Strand: Reverse

Name: dhbE [H]

Synonym: Bpro_4125

Alternate gene names: 91789964

Gene position: 4331548-4329878 (Counterclockwise)

Preceding gene: 91789965

Following gene: 91789963

Centisome position: 83.29

GC content: 66.55

Gene sequence:

>1671_bases
ATGCGAGACATTCAAAGCCCCATCGAGGGCGTGACCTACCCGTCCGACCAACGCGCCGCCGCCTCGCTGGCGAATGGTTC
CTGGGTGGCCTCCACCGTGGGCGACTGCCTGCGCGCCACGGCGGCGCGCTACCCCGACAGGCAGGCCTTTATCAGCGATG
AGCGAAGCCTGACTTTTCGCGAACTGGACGAGACAACGGATCGCCTGGCCGCTGCGCTGCTGGCCATGGGGCTGGCGACG
GGCGACCGCGCCATCTTCCAGCTCGGCACGACGGTGGATACCGCCATCGTGCTGCTGGCCTGCTACAAGGCCGGCATCGT
GCCGGTTTGCTCGCTGCCGCAGCACCGCGAGGTTGAAATCGGCCAGCTCGCCGCCCAGTCGGGCGCCCGCGGCTATTTTG
TGCAGACCGATTTCGGCAGTTTCGACCTGGTCGGTTTAGCCCGGACCATGGCGGCCCGGCACCCGTCACTGGAACATCTG
GTCGTGGTGCGCGGCGCGGTCGCCGGCCTGCCCGGCATCGACGCATTGATCGCCGACATGCCCCTTGAGCAGGCGCGGCA
GCAGTTGGGCCATATTGCGCTCGGCATGGAAGACGTGCTGAGCTTCCAGCTTTCCGGCGGCACCACCGGTGTGCCCAAAA
TCATTCCGCGCTTCCATGCCGAATACCTGGGGCATTCCGCCGCATGGATGCGGCGCTACCGCATCGACGCCGGCAGCCGC
GTGATCTGGTCCCTGCCGCTGCTGCACAACGCCGGCCAGCTGTACACACTGATGCCCGCGGCCCTGATGGGCGTGACCGT
GGTGCTGATGCCCAGGGTGGATATTGCCCGCATGCTGGCGCTGATTGAAGAACACCGCATCACGCATGCCTTGTCGATCG
GCCCGATCGCGCCGCAGCTGCTGGCGTATACCGACATCGCCAGGCACGACCTGTCGTCGCTGCAGCTCTTTGCCACCATG
AGCCGCGCCGACAAGCTGGAAAAGCACATTGGCGTGCCTTGCTCCAACCTGTACGGCATCACCGAAGGGCTGCTGCTCGG
CTCGCCCGCCGATGCGCCGGCCTTTGCGCGGCACCACACGCAAGGCACTTCGGGTTGCGCGCACGACGAGATCCGCCTGC
TGGTGCCCGAAACCGAAGAACCGAGTCCGCCCGGGGAGATGGGCGAGATGTGCTTTCGCGGCCCGTCCAGCCTGACCGGC
TTCTTCGGCAATCCCGAGGCGAACGCCAAAGCCTTCACCTCCGACGGCTTCTACCGCACCGGCGACATGATGACCGCGCA
CGTCGTCGACGGCGTCACCTGCTATGCCTTTGAAGGCCGGCTGCGCGACAACGTCAACCGCGGCGGCGAAAAAATCGGCT
GCGAAGAGGTCGAAGGCTTCGTCAGCCAGCACCCGGCCGTGGCCGACGCCAAGCTGGTTGCCATGCCCGACCCGTTCTAC
GGCGAGAAGGCCTGCGTCTTCATTGTTCCGCGCCCCGGCATGGTTGCACCCGACGTGAAGGCGCTCGGCGCCTTCCTCGT
CGGCCTGGGCCTGGCCAAATACAAGTGCCCGGAACGGGTCGAGATCGTTGACAGCTACCCGCTGACGCGCGTGGGCAAGA
TCGACAAGCCCGCATTGAAGCAGCGCATCGCCGACCAGCTGGCCGCCGAGGCGGCAGGGAGCCCGTCATGA

Upstream 100 bases:

>100_bases
CACATTGAAGTGTTTGAGCGGGACCGTGTGGTCATTGCACCGGCCCGGCCCTGAGCACACGCTGAAACCCAAGAGAACCA
AAGAGACCCCGGAGACAAGC

Downstream 100 bases:

>100_bases
TCGCCACCGAGCAGGTGCTGGGCGAGCGGCCGCTGGTGATTCGCCGCCGCGTCAAGTGGGGCGATTGCGACCCGGCGGGC
GTGGTCTACACGCCGATGTT

Product: AMP-dependent synthetase and ligase

Products: (2,3-Dihydroxybenzoyl)adenylate; Diphosphate [C]

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MRDIQSPIEGVTYPSDQRAAASLANGSWVASTVGDCLRATAARYPDRQAFISDERSLTFRELDETTDRLAAALLAMGLAT
GDRAIFQLGTTVDTAIVLLACYKAGIVPVCSLPQHREVEIGQLAAQSGARGYFVQTDFGSFDLVGLARTMAARHPSLEHL
VVVRGAVAGLPGIDALIADMPLEQARQQLGHIALGMEDVLSFQLSGGTTGVPKIIPRFHAEYLGHSAAWMRRYRIDAGSR
VIWSLPLLHNAGQLYTLMPAALMGVTVVLMPRVDIARMLALIEEHRITHALSIGPIAPQLLAYTDIARHDLSSLQLFATM
SRADKLEKHIGVPCSNLYGITEGLLLGSPADAPAFARHHTQGTSGCAHDEIRLLVPETEEPSPPGEMGEMCFRGPSSLTG
FFGNPEANAKAFTSDGFYRTGDMMTAHVVDGVTCYAFEGRLRDNVNRGGEKIGCEEVEGFVSQHPAVADAKLVAMPDPFY
GEKACVFIVPRPGMVAPDVKALGAFLVGLGLAKYKCPERVEIVDSYPLTRVGKIDKPALKQRIADQLAAEAAGSPS

Sequences:

>Translated_556_residues
MRDIQSPIEGVTYPSDQRAAASLANGSWVASTVGDCLRATAARYPDRQAFISDERSLTFRELDETTDRLAAALLAMGLAT
GDRAIFQLGTTVDTAIVLLACYKAGIVPVCSLPQHREVEIGQLAAQSGARGYFVQTDFGSFDLVGLARTMAARHPSLEHL
VVVRGAVAGLPGIDALIADMPLEQARQQLGHIALGMEDVLSFQLSGGTTGVPKIIPRFHAEYLGHSAAWMRRYRIDAGSR
VIWSLPLLHNAGQLYTLMPAALMGVTVVLMPRVDIARMLALIEEHRITHALSIGPIAPQLLAYTDIARHDLSSLQLFATM
SRADKLEKHIGVPCSNLYGITEGLLLGSPADAPAFARHHTQGTSGCAHDEIRLLVPETEEPSPPGEMGEMCFRGPSSLTG
FFGNPEANAKAFTSDGFYRTGDMMTAHVVDGVTCYAFEGRLRDNVNRGGEKIGCEEVEGFVSQHPAVADAKLVAMPDPFY
GEKACVFIVPRPGMVAPDVKALGAFLVGLGLAKYKCPERVEIVDSYPLTRVGKIDKPALKQRIADQLAAEAAGSPS
>Mature_556_residues
MRDIQSPIEGVTYPSDQRAAASLANGSWVASTVGDCLRATAARYPDRQAFISDERSLTFRELDETTDRLAAALLAMGLAT
GDRAIFQLGTTVDTAIVLLACYKAGIVPVCSLPQHREVEIGQLAAQSGARGYFVQTDFGSFDLVGLARTMAARHPSLEHL
VVVRGAVAGLPGIDALIADMPLEQARQQLGHIALGMEDVLSFQLSGGTTGVPKIIPRFHAEYLGHSAAWMRRYRIDAGSR
VIWSLPLLHNAGQLYTLMPAALMGVTVVLMPRVDIARMLALIEEHRITHALSIGPIAPQLLAYTDIARHDLSSLQLFATM
SRADKLEKHIGVPCSNLYGITEGLLLGSPADAPAFARHHTQGTSGCAHDEIRLLVPETEEPSPPGEMGEMCFRGPSSLTG
FFGNPEANAKAFTSDGFYRTGDMMTAHVVDGVTCYAFEGRLRDNVNRGGEKIGCEEVEGFVSQHPAVADAKLVAMPDPFY
GEKACVFIVPRPGMVAPDVKALGAFLVGLGLAKYKCPERVEIVDSYPLTRVGKIDKPALKQRIADQLAAEAAGSPS

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=580, Percent_Identity=25.8620689655172, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI38505220, Length=510, Percent_Identity=28.2352941176471, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI58082049, Length=517, Percent_Identity=24.9516441005803, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI115511026, Length=523, Percent_Identity=26.3862332695985, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI157311624, Length=517, Percent_Identity=23.9845261121857, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI157311622, Length=517, Percent_Identity=23.9845261121857, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI42544132, Length=530, Percent_Identity=23.2075471698113, Blast_Score=97, Evalue=6e-20,
Organism=Homo sapiens, GI187761345, Length=556, Percent_Identity=24.2805755395683, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI187761343, Length=556, Percent_Identity=24.2805755395683, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI28416953, Length=552, Percent_Identity=25, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI122937307, Length=514, Percent_Identity=25.0972762645914, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI40807357, Length=483, Percent_Identity=25.0517598343685, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1786810, Length=546, Percent_Identity=32.0512820512821, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI145693145, Length=547, Percent_Identity=29.7989031078611, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI1788107, Length=535, Percent_Identity=26.1682242990654, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI221142682, Length=495, Percent_Identity=23.4343434343434, Blast_Score=111, Evalue=1e-25,
Organism=Escherichia coli, GI1786529, Length=528, Percent_Identity=24.4318181818182, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17560308, Length=587, Percent_Identity=25.7240204429302, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32563687, Length=542, Percent_Identity=22.3247232472325, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17558820, Length=505, Percent_Identity=23.1683168316832, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71994694, Length=551, Percent_Identity=24.1379310344828, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71994703, Length=551, Percent_Identity=24.1379310344828, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI71994690, Length=551, Percent_Identity=24.1379310344828, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17557194, Length=559, Percent_Identity=24.1502683363148, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17538037, Length=571, Percent_Identity=22.2416812609457, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17531443, Length=551, Percent_Identity=23.049001814882, Blast_Score=92, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17559526, Length=542, Percent_Identity=21.7712177121771, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560140, Length=539, Percent_Identity=21.5213358070501, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=349, Percent_Identity=25.7879656160458, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24581924, Length=550, Percent_Identity=22.7272727272727, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI21355181, Length=494, Percent_Identity=23.0769230769231, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21358303, Length=465, Percent_Identity=23.2258064516129, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28573631, Length=552, Percent_Identity=23.731884057971, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI20130353, Length=552, Percent_Identity=23.731884057971, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI21356441, Length=483, Percent_Identity=22.7743271221532, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI18859661, Length=362, Percent_Identity=23.2044198895028, Blast_Score=72, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58; 2.3.1.- [C]

Molecular weight: Translated: 59656; Mature: 59656

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00455 AMP_BINDING ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDIQSPIEGVTYPSDQRAAASLANGSWVASTVGDCLRATAARYPDRQAFISDERSLTFR
CCCHHCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHH
ELDETTDRLAAALLAMGLATGDRAIFQLGTTVDTAIVLLACYKAGIVPVCSLPQHREVEI
HHHHHHHHHHHHHHHHHHCCCCHHHEEECCHHHHHHHHHHHHHCCCCEECCCCCCCCCHH
GQLAAQSGARGYFVQTDFGSFDLVGLARTMAARHPSLEHLVVVRGAVAGLPGIDALIADM
HHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHCC
PLEQARQQLGHIALGMEDVLSFQLSGGTTGVPKIIPRFHAEYLGHSAAWMRRYRIDAGSR
CHHHHHHHHHHHHCCHHHHHEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCE
VIWSLPLLHNAGQLYTLMPAALMGVTVVLMPRVDIARMLALIEEHRITHALSIGPIAPQL
EEEEECHHHCCCCHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHEECCCCCHHH
LAYTDIARHDLSSLQLFATMSRADKLEKHIGVPCSNLYGITEGLLLGSPADAPAFARHHT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEECCCCCCCHHHHHCC
QGTSGCAHDEIRLLVPETEEPSPPGEMGEMCFRGPSSLTGFFGNPEANAKAFTSDGFYRT
CCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCEEC
GDMMTAHVVDGVTCYAFEGRLRDNVNRGGEKIGCEEVEGFVSQHPAVADAKLVAMPDPFY
CCCEEEHHHCCEEEEEECCHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCC
GEKACVFIVPRPGMVAPDVKALGAFLVGLGLAKYKCPERVEIVDSYPLTRVGKIDKPALK
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHEEECCCCCCCCCCCCCHHHH
QRIADQLAAEAAGSPS
HHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRDIQSPIEGVTYPSDQRAAASLANGSWVASTVGDCLRATAARYPDRQAFISDERSLTFR
CCCHHCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCHHHHCCCCCCHHH
ELDETTDRLAAALLAMGLATGDRAIFQLGTTVDTAIVLLACYKAGIVPVCSLPQHREVEI
HHHHHHHHHHHHHHHHHHCCCCHHHEEECCHHHHHHHHHHHHHCCCCEECCCCCCCCCHH
GQLAAQSGARGYFVQTDFGSFDLVGLARTMAARHPSLEHLVVVRGAVAGLPGIDALIADM
HHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHCC
PLEQARQQLGHIALGMEDVLSFQLSGGTTGVPKIIPRFHAEYLGHSAAWMRRYRIDAGSR
CHHHHHHHHHHHHCCHHHHHEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCE
VIWSLPLLHNAGQLYTLMPAALMGVTVVLMPRVDIARMLALIEEHRITHALSIGPIAPQL
EEEEECHHHCCCCHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHEECCCCCHHH
LAYTDIARHDLSSLQLFATMSRADKLEKHIGVPCSNLYGITEGLLLGSPADAPAFARHHT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCEECCCCCCCHHHHHCC
QGTSGCAHDEIRLLVPETEEPSPPGEMGEMCFRGPSSLTGFFGNPEANAKAFTSDGFYRT
CCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEECCCCEEC
GDMMTAHVVDGVTCYAFEGRLRDNVNRGGEKIGCEEVEGFVSQHPAVADAKLVAMPDPFY
CCCEEEHHHCCEEEEEECCHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCC
GEKACVFIVPRPGMVAPDVKALGAFLVGLGLAKYKCPERVEIVDSYPLTRVGKIDKPALK
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHEEECCCCCCCCCCCCCHHHH
QRIADQLAAEAAGSPS
HHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,3-Dihydroxybenzoate; ATP [C]

Specific reaction: 2,3-Dihydroxybenzoate + ATP <==> (2,3-Dihydroxybenzoyl)adenylate + Diphosphate [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]