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The map label for this gene is gra-orf6 [H]

Identifier: 91789926

GI number: 91789926

Start: 4297018

End: 4297785

Strand: Direct

Name: gra-orf6 [H]

Synonym: Bpro_4087

Alternate gene names: 91789926

Gene position: 4297018-4297785 (Clockwise)

Preceding gene: 91789922

Following gene: 91789927

Centisome position: 82.63

GC content: 68.23

Gene sequence:

>768_bases
ATGACCGCCACCCCGTCCCTCCCCGTTGTTCTCGTGACCGGCGCCGCCAGGCGGCTGGGCCGCGAGATCGCGCTGGCGCT
GGCAGCCGGCGGCTGGCAGGTGGCGGTGCATTACCGCGGCTCCGAAGAAGATGCCCTGAAAACGGTCGCTGATTGCACCC
GTTTGTCGGGGGCTGCCGCCGCTTTTCAGTGTGACCTGGAACAGGAAGCCGGCGTTCGCGCCCTGCTGCCCCGCGTTGCC
GCGCATTTCGGCCGCGTTGACGCGGTGGTCAACAACGCCTCGACCTTTGAGCAGGACAGCGCCGCCAGCTTTGGCTTTGC
CGCCCTGGACAAGCACCTGCACAGCAATACCGGCGCGGCCATCCTGCTGGCCCAGGCGCTGCATGCGCATGTGAGCAGCC
GCAACGCCAGCGGCGCCGTCGTCAACCTGCTGGACCAGAAGCTGTGGAACCAGAACCCCGATTTCTTCAGCTACACCCTG
TCCAAGGCCGCGCTGGAGGCGGCCGGTACCATGCTGGCGCTGGCGCTGGCGCCGCAGGTGCGCGTGGTCGGCGTGGCGCC
CGGCCTCACCCTGACCAGCCACCTGCTGAGCGACGAGCAATTCGAGGCGCGGCACAAGCTGTCGCCGCTGGGCCGCTCCT
CCACGCCGGCCGACGTGGCTGCCGCGGTGAAGTTCGCGCTGGAGAACAGCTCCATGACAGGCACCACCCTGCTGGTGGAC
GGCGGCCAGCACCTGATGAAATTTGAGCGCGACTTCTCGCTGATGTGA

Upstream 100 bases:

>100_bases
AAAATGGGGGGGTGGCTGTTGTTGGTCACGCCCCTGATTGTCGGCGATACCGCCCTGGCCCCGCCACCAGCCCCTGCCCA
AAAACCCAACCTCCAGCCTG

Downstream 100 bases:

>100_bases
ACGCAAACGCTGGAAAACCGCCTGCACACCCCGGCCCCAATCACCCCCAGACTGACCCCATGTACAACACCCTAGGCACC
CAGATCCTGACGCTCACCGG

Product: short-chain dehydrogenase/reductase SDR

Products: @DEHY06.txt*3-Dehydro-2- Deoxy-D-Gluconate!; NADH. [C]

Alternate protein names: ORF6 [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MTATPSLPVVLVTGAARRLGREIALALAAGGWQVAVHYRGSEEDALKTVADCTRLSGAAAAFQCDLEQEAGVRALLPRVA
AHFGRVDAVVNNASTFEQDSAASFGFAALDKHLHSNTGAAILLAQALHAHVSSRNASGAVVNLLDQKLWNQNPDFFSYTL
SKAALEAAGTMLALALAPQVRVVGVAPGLTLTSHLLSDEQFEARHKLSPLGRSSTPADVAAAVKFALENSSMTGTTLLVD
GGQHLMKFERDFSLM

Sequences:

>Translated_255_residues
MTATPSLPVVLVTGAARRLGREIALALAAGGWQVAVHYRGSEEDALKTVADCTRLSGAAAAFQCDLEQEAGVRALLPRVA
AHFGRVDAVVNNASTFEQDSAASFGFAALDKHLHSNTGAAILLAQALHAHVSSRNASGAVVNLLDQKLWNQNPDFFSYTL
SKAALEAAGTMLALALAPQVRVVGVAPGLTLTSHLLSDEQFEARHKLSPLGRSSTPADVAAAVKFALENSSMTGTTLLVD
GGQHLMKFERDFSLM
>Mature_254_residues
TATPSLPVVLVTGAARRLGREIALALAAGGWQVAVHYRGSEEDALKTVADCTRLSGAAAAFQCDLEQEAGVRALLPRVAA
HFGRVDAVVNNASTFEQDSAASFGFAALDKHLHSNTGAAILLAQALHAHVSSRNASGAVVNLLDQKLWNQNPDFFSYTLS
KAALEAAGTMLALALAPQVRVVGVAPGLTLTSHLLSDEQFEARHKLSPLGRSSTPADVAAAVKFALENSSMTGTTLLVDG
GQHLMKFERDFSLM

Specific function: Unknown

COG id: COG1028

COG function: function code IQR; Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI40254992, Length=240, Percent_Identity=31.25, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI7705925, Length=243, Percent_Identity=30.4526748971193, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1789208, Length=242, Percent_Identity=28.9256198347107, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI2367175, Length=247, Percent_Identity=28.7449392712551, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787891, Length=258, Percent_Identity=27.906976744186, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787335, Length=249, Percent_Identity=28.1124497991968, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1788459, Length=239, Percent_Identity=25.9414225941423, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787905, Length=238, Percent_Identity=30.2521008403361, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790717, Length=242, Percent_Identity=26.8595041322314, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71994600, Length=256, Percent_Identity=29.6875, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI71994604, Length=261, Percent_Identity=31.0344827586207, Blast_Score=84, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17562910, Length=261, Percent_Identity=26.0536398467433, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115534694, Length=240, Percent_Identity=30.8333333333333, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17562906, Length=260, Percent_Identity=27.6923076923077, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17544670, Length=264, Percent_Identity=25.7575757575758, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17562904, Length=262, Percent_Identity=26.3358778625954, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI193204405, Length=264, Percent_Identity=26.8939393939394, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=260, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17559104, Length=260, Percent_Identity=26.5384615384615, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17551412, Length=267, Percent_Identity=26.9662921348315, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17562908, Length=268, Percent_Identity=26.4925373134328, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17560220, Length=264, Percent_Identity=25.7575757575758, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI28571526, Length=252, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI23397609, Length=248, Percent_Identity=32.258064516129, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21357041, Length=252, Percent_Identity=31.3492063492063, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24644339, Length=250, Percent_Identity=29.6, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24644337, Length=217, Percent_Identity=27.6497695852535, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.125 [C]

Molecular weight: Translated: 26772; Mature: 26640

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATPSLPVVLVTGAARRLGREIALALAAGGWQVAVHYRGSEEDALKTVADCTRLSGAAA
CCCCCCCCEEEEEHHHHHHHHHHHHHEECCCEEEEEEECCCCHHHHHHHHHHHHHCCCHH
AFQCDLEQEAGVRALLPRVAAHFGRVDAVVNNASTFEQDSAASFGFAALDKHLHSNTGAA
EEEECCHHHCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHCCCCHH
ILLAQALHAHVSSRNASGAVVNLLDQKLWNQNPDFFSYTLSKAALEAAGTMLALALAPQV
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
RVVGVAPGLTLTSHLLSDEQFEARHKLSPLGRSSTPADVAAAVKFALENSSMTGTTLLVD
EEEEECCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEE
GGQHLMKFERDFSLM
CCHHHHHHHHHCCCC
>Mature Secondary Structure 
TATPSLPVVLVTGAARRLGREIALALAAGGWQVAVHYRGSEEDALKTVADCTRLSGAAA
CCCCCCCEEEEEHHHHHHHHHHHHHEECCCEEEEEEECCCCHHHHHHHHHHHHHCCCHH
AFQCDLEQEAGVRALLPRVAAHFGRVDAVVNNASTFEQDSAASFGFAALDKHLHSNTGAA
EEEECCHHHCCHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCHHHHHHHHHHCCCCHH
ILLAQALHAHVSSRNASGAVVNLLDQKLWNQNPDFFSYTLSKAALEAAGTMLALALAPQV
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCE
RVVGVAPGLTLTSHLLSDEQFEARHKLSPLGRSSTPADVAAAVKFALENSSMTGTTLLVD
EEEEECCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEE
GGQHLMKFERDFSLM
CCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 15.4 {2-deoxygluconate}} [C]

Substrates: @DEOX03.txt*2-Deoxy-D-Gluconate!; NAD(+) [C]

Specific reaction: @DEOX03.txt*2-Deoxy-D-Gluconate! + NAD(+) = @DEHY06.txt*3-Dehydro-2- Deoxy-D-Gluconate! + NADH. [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2583128; 9831526 [H]