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The map label for this gene is tgs1 [H]

Identifier: 91789881

GI number: 91789881

Start: 4242712

End: 4244163

Strand: Direct

Name: tgs1 [H]

Synonym: Bpro_4042

Alternate gene names: 91789881

Gene position: 4242712-4244163 (Clockwise)

Preceding gene: 91789879

Following gene: 91789882

Centisome position: 81.59

GC content: 67.08

Gene sequence:

>1452_bases
ATGAGCAAGGTCGACACCGCCTGGCTGCGCATGGACAGCGCCGCCAACCTGATGATGATTGTCGGCGTGTGGCAGCTGGC
GCCGGGCGTCACGTACGCCGCGGTGTGCGAGCGCATCGAGGGCAGCCTGCTCAGGTACGACCGCTTCCGGCAGCGCGTGG
TCGAGGATGCGGCGGGCGCCACCTGGGTGAACGCCCGCAACTTTGACCTCGCCCGGCATGTGGTGCTGGAAAAGCTGCCG
CCAGCCACCAGGACCGCGCAGGGCCACCAACAGGAAGCGCTGCAGGACCGCGTGGCCGAGCTCGCGGCCCAGCCACTGGA
CCGCAAGCACCCGCTCTGGCAGATCCACCTGATCGAGGACTACACCGGTGCGGATGGCGTGAAGGGCAGCGCCATGATTG
TGCGCATACACCACTGCATTGGTGACGGCATTGCGCTGATCTCGGTGACGATGTCACTGGTCGACGGCGGCGCACCGCCG
CCCGAGCGCCGCCAGAAGGCTGCCGGCACCGGCAGCGCGGAAGACTGGATCACCGACACCCTGCTCAAGCCGTTCACCGA
CATCACCGTCAAGGCGCTGGGCGCGGTGGGCGAAGGCGCAGCGCGCTCGCTGGGCCTGCTGGGCAATCCGAAAATGGGCA
TGGCGCAGGGCCTGGAAAAAGGCGTGAGCGGCTCGCTCGACATGGCCAGGGTGCTGCTCCAGTTGGTCTCGGACTCGGCC
GCGCTGGCCCTGATGCCGGACGATGCCAAAACCCGGCTCAAGGGCAAGCCCGGCGGCGCCAAGAAGGTCGCTTGGTGCCA
GCCGATACCGCTGGACGAGGTCAAGGCCGTCGGCAAGGCGCTCAGCTGCTCCATCAACGATGTGCTCCTCAGCTGTGTGG
CCGGGGCGATCGGCGAATACCTCAAGACCTGTGGCGACGATGTGGCGGGCCAGGAAATCCGCGCCATGGTGCCGGTGAAC
CTGCGCCCCCTGGAGCATGCCCACAAGCTCGGCAACCGCTTTGGCCTGGTGCCGCTGCTGCTGCCCATCGGCATGGAGAA
CCCGGTCGAGCGCGTGTACGAGGTGCGCCGCCGCATGGCGGCCCTGAAGGGCAGCTACCAGCCGCTGCTGGCCTTCAGCC
TGCTGGCCGTGGCGGGCCTGCTGATCAAGCCGGCGCAGGACATGATGCTCAACCTGTTTGCCAAAAAAACGACGGCCGTG
ATGACCAACGTGCCGGGCCCGCGCGAAAAGCTCAGGTTCTGCGGCGCCACGCTGGAGCAAAGCATGTTCTGGGTGCCGCA
GTCGGGCGACGTGGGGCTGGGTGTGTCCATCCTCAGCTACGGCGGCGGTGTGCAGTTTGGCGTGATCACCGACAGCCTGC
TGTGCCCGGAGCCGCAGCGCATCATTGATGAGTTTGTGCCCGAGTTTGCCAAGCTGTCCATCGTGACGCTGATGCTGCCC
TGGGGCGAGTAG

Upstream 100 bases:

>100_bases
CCGGGAAGACGTTCAGGGAGGCGCTGGCAGCGTTCAGAAAAACCTTCAGGCCAATATCAGGCGGGCCGTCTCCGGCACGC
TGGGCCTGTCGGGCGAGCGC

Downstream 100 bases:

>100_bases
TCCCCAAGACGGATTTGGTGAGGGCGATTCCGGCGTCAAGGCTCCCGGGCTCTCTTGCAGCACTGTTGCTGTTTTGCGAA
CCGCATGCGTCGCTTCATGC

Product: diacylglycerol O-acyltransferase

Products: NA

Alternate protein names: TGS1; Probable triacylglycerol synthase tgs1 [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSKVDTAWLRMDSAANLMMIVGVWQLAPGVTYAAVCERIEGSLLRYDRFRQRVVEDAAGATWVNARNFDLARHVVLEKLP
PATRTAQGHQQEALQDRVAELAAQPLDRKHPLWQIHLIEDYTGADGVKGSAMIVRIHHCIGDGIALISVTMSLVDGGAPP
PERRQKAAGTGSAEDWITDTLLKPFTDITVKALGAVGEGAARSLGLLGNPKMGMAQGLEKGVSGSLDMARVLLQLVSDSA
ALALMPDDAKTRLKGKPGGAKKVAWCQPIPLDEVKAVGKALSCSINDVLLSCVAGAIGEYLKTCGDDVAGQEIRAMVPVN
LRPLEHAHKLGNRFGLVPLLLPIGMENPVERVYEVRRRMAALKGSYQPLLAFSLLAVAGLLIKPAQDMMLNLFAKKTTAV
MTNVPGPREKLRFCGATLEQSMFWVPQSGDVGLGVSILSYGGGVQFGVITDSLLCPEPQRIIDEFVPEFAKLSIVTLMLP
WGE

Sequences:

>Translated_483_residues
MSKVDTAWLRMDSAANLMMIVGVWQLAPGVTYAAVCERIEGSLLRYDRFRQRVVEDAAGATWVNARNFDLARHVVLEKLP
PATRTAQGHQQEALQDRVAELAAQPLDRKHPLWQIHLIEDYTGADGVKGSAMIVRIHHCIGDGIALISVTMSLVDGGAPP
PERRQKAAGTGSAEDWITDTLLKPFTDITVKALGAVGEGAARSLGLLGNPKMGMAQGLEKGVSGSLDMARVLLQLVSDSA
ALALMPDDAKTRLKGKPGGAKKVAWCQPIPLDEVKAVGKALSCSINDVLLSCVAGAIGEYLKTCGDDVAGQEIRAMVPVN
LRPLEHAHKLGNRFGLVPLLLPIGMENPVERVYEVRRRMAALKGSYQPLLAFSLLAVAGLLIKPAQDMMLNLFAKKTTAV
MTNVPGPREKLRFCGATLEQSMFWVPQSGDVGLGVSILSYGGGVQFGVITDSLLCPEPQRIIDEFVPEFAKLSIVTLMLP
WGE
>Mature_482_residues
SKVDTAWLRMDSAANLMMIVGVWQLAPGVTYAAVCERIEGSLLRYDRFRQRVVEDAAGATWVNARNFDLARHVVLEKLPP
ATRTAQGHQQEALQDRVAELAAQPLDRKHPLWQIHLIEDYTGADGVKGSAMIVRIHHCIGDGIALISVTMSLVDGGAPPP
ERRQKAAGTGSAEDWITDTLLKPFTDITVKALGAVGEGAARSLGLLGNPKMGMAQGLEKGVSGSLDMARVLLQLVSDSAA
LALMPDDAKTRLKGKPGGAKKVAWCQPIPLDEVKAVGKALSCSINDVLLSCVAGAIGEYLKTCGDDVAGQEIRAMVPVNL
RPLEHAHKLGNRFGLVPLLLPIGMENPVERVYEVRRRMAALKGSYQPLLAFSLLAVAGLLIKPAQDMMLNLFAKKTTAVM
TNVPGPREKLRFCGATLEQSMFWVPQSGDVGLGVSILSYGGGVQFGVITDSLLCPEPQRIIDEFVPEFAKLSIVTLMLPW
GE

Specific function: Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Prefers C(26:0)-CoA over C(18:1)-CoA. TG synthesis activity increases in M.tuberculosis upon oxygen depletion and NO

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 51978; Mature: 51847

Theoretical pI: Translated: 7.76; Mature: 7.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVDTAWLRMDSAANLMMIVGVWQLAPGVTYAAVCERIEGSLLRYDRFRQRVVEDAAGA
CCCCCCHHHEECCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TWVNARNFDLARHVVLEKLPPATRTAQGHQQEALQDRVAELAAQPLDRKHPLWQIHLIED
EEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEC
YTGADGVKGSAMIVRIHHCIGDGIALISVTMSLVDGGAPPPERRQKAAGTGSAEDWITDT
CCCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHH
LLKPFTDITVKALGAVGEGAARSLGLLGNPKMGMAQGLEKGVSGSLDMARVLLQLVSDSA
HHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
ALALMPDDAKTRLKGKPGGAKKVAWCQPIPLDEVKAVGKALSCSINDVLLSCVAGAIGEY
EEEECCCCHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LKTCGDDVAGQEIRAMVPVNLRPLEHAHKLGNRFGLVPLLLPIGMENPVERVYEVRRRMA
HHHHCCCCCCCHHEEECCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHH
ALKGSYQPLLAFSLLAVAGLLIKPAQDMMLNLFAKKTTAVMTNVPGPREKLRFCGATLEQ
HHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHH
SMFWVPQSGDVGLGVSILSYGGGVQFGVITDSLLCPEPQRIIDEFVPEFAKLSIVTLMLP
CEEECCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEEC
WGE
CCC
>Mature Secondary Structure 
SKVDTAWLRMDSAANLMMIVGVWQLAPGVTYAAVCERIEGSLLRYDRFRQRVVEDAAGA
CCCCCHHHEECCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TWVNARNFDLARHVVLEKLPPATRTAQGHQQEALQDRVAELAAQPLDRKHPLWQIHLIED
EEECCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEC
YTGADGVKGSAMIVRIHHCIGDGIALISVTMSLVDGGAPPPERRQKAAGTGSAEDWITDT
CCCCCCCCCCEEEEEEHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHH
LLKPFTDITVKALGAVGEGAARSLGLLGNPKMGMAQGLEKGVSGSLDMARVLLQLVSDSA
HHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
ALALMPDDAKTRLKGKPGGAKKVAWCQPIPLDEVKAVGKALSCSINDVLLSCVAGAIGEY
EEEECCCCHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LKTCGDDVAGQEIRAMVPVNLRPLEHAHKLGNRFGLVPLLLPIGMENPVERVYEVRRRMA
HHHHCCCCCCCHHEEECCCCCCCHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHH
ALKGSYQPLLAFSLLAVAGLLIKPAQDMMLNLFAKKTTAVMTNVPGPREKLRFCGATLEQ
HHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHH
SMFWVPQSGDVGLGVSILSYGGGVQFGVITDSLLCPEPQRIIDEFVPEFAKLSIVTLMLP
CEEECCCCCCCCCCHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEEEEC
WGE
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]