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The map label for this gene is rfbJ [H]

Identifier: 91789846

GI number: 91789846

Start: 4204005

End: 4204904

Strand: Direct

Name: rfbJ [H]

Synonym: Bpro_4006

Alternate gene names: 91789846

Gene position: 4204005-4204904 (Clockwise)

Preceding gene: 91789845

Following gene: 91789847

Centisome position: 80.84

GC content: 49.56

Gene sequence:

>900_bases
ATGAAAGTTCTTCTTACCGGCGCTACAGGCTTTCTCGGTACTCATCTTTTAGCTGATTTTCTAAACCGCAATTACAAAGT
TCTCATGACTAAACGGGCAAGCACAGACTTGGGCCCACTTTTTCTGCGTTTTGGTAACTTGGAAGCGTGGGATACCGAGG
AGTTGGAAAACATCTTCACTACGCACCCTGATGTTGAGGCGATAGTTCATGCTGCCACGGATTATGGCCGTGACAACTCA
AGCCCGACAGCAGTCTTTTGGGCTAATGAAGCGTTCCCGATAAAATTGATTGAACTTGCAATCCGGCATAACATCGCGCT
ATTTATCAACATGGATACCTTCTTTAACTCCGATAAAGTGTCGTACGACTATCTCGGAGCCTACACATTATCCAAACGCC
ACTTTCAAGAATGGGGGCAACATTGCGCCAACACAGGGCGAATCGGTTTTGCCAATCTGCGGCTATTTCATCTATATGGC
CCGGGAGATGGTCGCCAAAAATTCGTTCCGGCCATGGTGGGACGTTGCCTGGCTGGTGAGGAAATTGACCTTACGGACGG
TAAGCAGAAGCGCGACTTCATCCATATTGATGATGCTGTTGCAGCTGTGAGTACTGTTTTGGAGGCGGAGGCAGGACGAG
GGGGAGGCTATCGGCATTACGATGTGGGAACCGGCACCTCTCTGCGCATTCGGGACTTCATTGAAACGGTGAAAAGGCTT
TGCTGCAGCAGCGCGAAGTTAAATTTTGGTGCGTTACCAAACAGGAAAGGTGAGTTTCAGAATGCCTGCGCCGAAACTGA
AGCGTTGCGTACCCTTGGGTGGATACCGCGGGTTGGCATCGAAGCGGGAATACGGACAGTGATCGAGGATATCGGTCAAC
GGGCAATAAAAACTACATAG

Upstream 100 bases:

>100_bases
GCTGGATGCCCGAGGTTGCAAAGGTCGTTCAAATAGGGATCGTGACGCCAGTTAGCTATGCGGTTCAAAAGCTCGCTATT
TTTCGGGCGAGGAGTGAAAT

Downstream 100 bases:

>100_bases
AAGTGGAATCGAAGTGACCCCAAAAAAACTAATCTCGCTCATTATTCCTGTCTTCAATGAAGAAGAGAATATCTTTCCCC
TTTACAAGGCAGTGACCCCT

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MKVLLTGATGFLGTHLLADFLNRNYKVLMTKRASTDLGPLFLRFGNLEAWDTEELENIFTTHPDVEAIVHAATDYGRDNS
SPTAVFWANEAFPIKLIELAIRHNIALFINMDTFFNSDKVSYDYLGAYTLSKRHFQEWGQHCANTGRIGFANLRLFHLYG
PGDGRQKFVPAMVGRCLAGEEIDLTDGKQKRDFIHIDDAVAAVSTVLEAEAGRGGGYRHYDVGTGTSLRIRDFIETVKRL
CCSSAKLNFGALPNRKGEFQNACAETEALRTLGWIPRVGIEAGIRTVIEDIGQRAIKTT

Sequences:

>Translated_299_residues
MKVLLTGATGFLGTHLLADFLNRNYKVLMTKRASTDLGPLFLRFGNLEAWDTEELENIFTTHPDVEAIVHAATDYGRDNS
SPTAVFWANEAFPIKLIELAIRHNIALFINMDTFFNSDKVSYDYLGAYTLSKRHFQEWGQHCANTGRIGFANLRLFHLYG
PGDGRQKFVPAMVGRCLAGEEIDLTDGKQKRDFIHIDDAVAAVSTVLEAEAGRGGGYRHYDVGTGTSLRIRDFIETVKRL
CCSSAKLNFGALPNRKGEFQNACAETEALRTLGWIPRVGIEAGIRTVIEDIGQRAIKTT
>Mature_299_residues
MKVLLTGATGFLGTHLLADFLNRNYKVLMTKRASTDLGPLFLRFGNLEAWDTEELENIFTTHPDVEAIVHAATDYGRDNS
SPTAVFWANEAFPIKLIELAIRHNIALFINMDTFFNSDKVSYDYLGAYTLSKRHFQEWGQHCANTGRIGFANLRLFHLYG
PGDGRQKFVPAMVGRCLAGEEIDLTDGKQKRDFIHIDDAVAAVSTVLEAEAGRGGGYRHYDVGTGTSLRIRDFIETVKRL
CCSSAKLNFGALPNRKGEFQNACAETEALRTLGWIPRVGIEAGIRTVIEDIGQRAIKTT

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 33251; Mature: 33251

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLLTGATGFLGTHLLADFLNRNYKVLMTKRASTDLGPLFLRFGNLEAWDTEELENIFT
CEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHEEECCCCCCCHHHHHHHHC
THPDVEAIVHAATDYGRDNSSPTAVFWANEAFPIKLIELAIRHNIALFINMDTFFNSDKV
CCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEEHHHCCCCCC
SYDYLGAYTLSKRHFQEWGQHCANTGRIGFANLRLFHLYGPGDGRQKFVPAMVGRCLAGE
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCC
EIDLTDGKQKRDFIHIDDAVAAVSTVLEAEAGRGGGYRHYDVGTGTSLRIRDFIETVKRL
EEECCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
CCSSAKLNFGALPNRKGEFQNACAETEALRTLGWIPRVGIEAGIRTVIEDIGQRAIKTT
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVLLTGATGFLGTHLLADFLNRNYKVLMTKRASTDLGPLFLRFGNLEAWDTEELENIFT
CEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHEEECCCCCCCHHHHHHHHC
THPDVEAIVHAATDYGRDNSSPTAVFWANEAFPIKLIELAIRHNIALFINMDTFFNSDKV
CCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEEHHHCCCCCC
SYDYLGAYTLSKRHFQEWGQHCANTGRIGFANLRLFHLYGPGDGRQKFVPAMVGRCLAGE
CHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHHCCC
EIDLTDGKQKRDFIHIDDAVAAVSTVLEAEAGRGGGYRHYDVGTGTSLRIRDFIETVKRL
EEECCCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHHHH
CCSSAKLNFGALPNRKGEFQNACAETEALRTLGWIPRVGIEAGIRTVIEDIGQRAIKTT
HHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1379320 [H]