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The map label for this gene is vipB [H]

Identifier: 91789836

GI number: 91789836

Start: 4191619

End: 4192728

Strand: Reverse

Name: vipB [H]

Synonym: Bpro_3996

Alternate gene names: 91789836

Gene position: 4192728-4191619 (Counterclockwise)

Preceding gene: 91789837

Following gene: 91789835

Centisome position: 80.63

GC content: 59.82

Gene sequence:

>1110_bases
ATGACCGCATACGAACAACTCCAGATTCGCCTGAAGAGCGAGCCCCGTGCCTGGCTCGTCACTGGGGTTGCCGGCTTTAT
TGGCAGCAATCTGCTGGAAGCTCTGCTCAAGCTGGGCCAGCGCGTGGTCGGACTGGACAACTTTGCCACCGGCTACCAGC
GCAACCTGGACGAAGTGCAAACGCTGGTCTCTCCTGAGCAGTGGGCCAATTTCCACTTCATTGAGGGCGACATCCGTAAC
CTGCCCGATTGCCAGCGTGCCATGTCTTGGCCCCCTCTCCCCACGGAAGACGGTAGGGGTGAGGGCTCTCCCGTCGACTA
CGTCCTTCACCAGGCAGCCCTGGGCAGCGTGCCCCGGTCACTGGCCGATCCCATCACCACCAACGCCGCCAACATCACGG
GTTTCCTGAACATGCTGGTGGCCGCACGCGACGCCGGGGTCAAAAGCTTTACCTACGCCGCCAGCAGCAGCACCTACGGG
GATCACCCGGCTCTGCCCAAGGTAGAGCACACCATCGGCAAACCGCTCAGCCCCTATGCGGTGACCAAATATGTGAACGA
GCTGTATGCCGAGGTATTTGCCCGCTGCTACGGCTTTAACACCATAGGTTTGCGCTACTTCAATGTGTTTGGTGCACGGC
AAAATCCCAGTGGGGCCTATGCCGCTGTGATCCCGAAATGGACCGCAGCGATGATGAAAGGGGAAGACGTGTTCATCAAC
GGCGATGGTGAAACCAGCCGGGACTTCTGCTACGTGGCCAACGCGGTGCAAGCCAACCTGTTGGCGGCGACCGTGAGTGA
TCTCCCTCTCCCCCTGGAAGAGGGCGGGGGTGAGGGCTCTCCTACCAACCAGGTGTACAACGTGGCCGTGGGTGATCGCA
CTACGCTGAACACCCTGTTTGGCCTGATACGTGACAACTTGATTGCACGTGGCGTGCCTGTCAGTACCGAGCCCAAGTAT
CGGGATTTCCGTGCGGGCGACGTGCGTAACAGCCTGGCCGACATCGGCAAGGCCCATCGGATGCTGGGTTACGCCCCTAC
CCATCGCATAGACCAGGGATTGCAGTTGGCGATTCCTTGGTATGTCCAGAACTGCAAGGTTTCATCATGA

Upstream 100 bases:

>100_bases
TTTGCCTGGTCCAGTGGTCAATCCGTGGGCAAATAGCGAGTGAAAAATGCTTTCTGCCCTTTCCATTTGAGTGCAGGTAG
CTATCAAAATCATAGTAGAA

Downstream 100 bases:

>100_bases
TCATCTGCGTCATGGGCCAACAGTACGCCTTATACATTGGAAGAACTTTTCGTGGAAATGGCACGAGAAGGCCTAAAATT
AACTGAGCGAAATGAATTGA

Product: NAD-dependent epimerase/dehydratase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MTAYEQLQIRLKSEPRAWLVTGVAGFIGSNLLEALLKLGQRVVGLDNFATGYQRNLDEVQTLVSPEQWANFHFIEGDIRN
LPDCQRAMSWPPLPTEDGRGEGSPVDYVLHQAALGSVPRSLADPITTNAANITGFLNMLVAARDAGVKSFTYAASSSTYG
DHPALPKVEHTIGKPLSPYAVTKYVNELYAEVFARCYGFNTIGLRYFNVFGARQNPSGAYAAVIPKWTAAMMKGEDVFIN
GDGETSRDFCYVANAVQANLLAATVSDLPLPLEEGGGEGSPTNQVYNVAVGDRTTLNTLFGLIRDNLIARGVPVSTEPKY
RDFRAGDVRNSLADIGKAHRMLGYAPTHRIDQGLQLAIPWYVQNCKVSS

Sequences:

>Translated_369_residues
MTAYEQLQIRLKSEPRAWLVTGVAGFIGSNLLEALLKLGQRVVGLDNFATGYQRNLDEVQTLVSPEQWANFHFIEGDIRN
LPDCQRAMSWPPLPTEDGRGEGSPVDYVLHQAALGSVPRSLADPITTNAANITGFLNMLVAARDAGVKSFTYAASSSTYG
DHPALPKVEHTIGKPLSPYAVTKYVNELYAEVFARCYGFNTIGLRYFNVFGARQNPSGAYAAVIPKWTAAMMKGEDVFIN
GDGETSRDFCYVANAVQANLLAATVSDLPLPLEEGGGEGSPTNQVYNVAVGDRTTLNTLFGLIRDNLIARGVPVSTEPKY
RDFRAGDVRNSLADIGKAHRMLGYAPTHRIDQGLQLAIPWYVQNCKVSS
>Mature_368_residues
TAYEQLQIRLKSEPRAWLVTGVAGFIGSNLLEALLKLGQRVVGLDNFATGYQRNLDEVQTLVSPEQWANFHFIEGDIRNL
PDCQRAMSWPPLPTEDGRGEGSPVDYVLHQAALGSVPRSLADPITTNAANITGFLNMLVAARDAGVKSFTYAASSSTYGD
HPALPKVEHTIGKPLSPYAVTKYVNELYAEVFARCYGFNTIGLRYFNVFGARQNPSGAYAAVIPKWTAAMMKGEDVFING
DGETSRDFCYVANAVQANLLAATVSDLPLPLEEGGGEGSPTNQVYNVAVGDRTTLNTLFGLIRDNLIARGVPVSTEPKYR
DFRAGDVRNSLADIGKAHRMLGYAPTHRIDQGLQLAIPWYVQNCKVSS

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=351, Percent_Identity=29.0598290598291, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI7657641, Length=351, Percent_Identity=25.9259259259259, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI56237023, Length=367, Percent_Identity=25.6130790190736, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI56118217, Length=367, Percent_Identity=25.6130790190736, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI189083684, Length=367, Percent_Identity=25.6130790190736, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1786974, Length=357, Percent_Identity=28.5714285714286, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788353, Length=374, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994969, Length=368, Percent_Identity=26.6304347826087, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17539532, Length=369, Percent_Identity=25.2032520325203, Blast_Score=92, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=367, Percent_Identity=25.8855585831063, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71982035, Length=354, Percent_Identity=26.5536723163842, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=354, Percent_Identity=26.5536723163842, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI115532424, Length=361, Percent_Identity=23.8227146814404, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=379, Percent_Identity=24.2744063324538, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21356223, Length=351, Percent_Identity=27.0655270655271, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19923002, Length=368, Percent_Identity=26.3586956521739, Blast_Score=88, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 40195; Mature: 40064

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAYEQLQIRLKSEPRAWLVTGVAGFIGSNLLEALLKLGQRVVGLDNFATGYQRNLDEVQ
CCCHHEEHEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
TLVSPEQWANFHFIEGDIRNLPDCQRAMSWPPLPTEDGRGEGSPVDYVLHQAALGSVPRS
HHHCHHHHCCEEEECCHHCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHH
LADPITTNAANITGFLNMLVAARDAGVKSFTYAASSSTYGDHPALPKVEHTIGKPLSPYA
HHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCHHH
VTKYVNELYAEVFARCYGFNTIGLRYFNVFGARQNPSGAYAAVIPKWTAAMMKGEDVFIN
HHHHHHHHHHHHHHHHHCCCHHHHEEEEEECCCCCCCCCEEEECCHHHHHHHCCCEEEEE
GDGETSRDFCYVANAVQANLLAATVSDLPLPLEEGGGEGSPTNQVYNVAVGDRTTLNTLF
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCEEEEECCCCHHHHHHH
GLIRDNLIARGVPVSTEPKYRDFRAGDVRNSLADIGKAHRMLGYAPTHRIDQGLQLAIPW
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEE
YVQNCKVSS
EECCCCCCC
>Mature Secondary Structure 
TAYEQLQIRLKSEPRAWLVTGVAGFIGSNLLEALLKLGQRVVGLDNFATGYQRNLDEVQ
CCHHEEHEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHH
TLVSPEQWANFHFIEGDIRNLPDCQRAMSWPPLPTEDGRGEGSPVDYVLHQAALGSVPRS
HHHCHHHHCCEEEECCHHCCCCHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHH
LADPITTNAANITGFLNMLVAARDAGVKSFTYAASSSTYGDHPALPKVEHTIGKPLSPYA
HHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCHHH
VTKYVNELYAEVFARCYGFNTIGLRYFNVFGARQNPSGAYAAVIPKWTAAMMKGEDVFIN
HHHHHHHHHHHHHHHHHCCCHHHHEEEEEECCCCCCCCCEEEECCHHHHHHHCCCEEEEE
GDGETSRDFCYVANAVQANLLAATVSDLPLPLEEGGGEGSPTNQVYNVAVGDRTTLNTLF
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCEEEEECCCCHHHHHHH
GLIRDNLIARGVPVSTEPKYRDFRAGDVRNSLADIGKAHRMLGYAPTHRIDQGLQLAIPW
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHCCCCEEEEEE
YVQNCKVSS
EECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8248629; 8331073; 11677608; 12644504 [H]