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The map label for this gene is asnB [H]

Identifier: 91789828

GI number: 91789828

Start: 4181594

End: 4183564

Strand: Reverse

Name: asnB [H]

Synonym: Bpro_3988

Alternate gene names: 91789828

Gene position: 4183564-4181594 (Counterclockwise)

Preceding gene: 91789829

Following gene: 91789827

Centisome position: 80.45

GC content: 63.57

Gene sequence:

>1971_bases
ATGTGCGGACTTGCAGGGTATTTCAGCGGCCAGTGGCCGGCCGATGGGGAGCAGGCCTCAGCCCGGCTCAAAGCCATGAC
CGATGCGATTGCCCACCGTGGCCCGGATTCGGATGGCTACTGGCTGGATGCGGCCACCTGTATGGCGCTGGGGCATCGCC
GCCTGGCAATTTTGGAGCTATCGGCCGCTGGTGCGCAGCCGATGCACGCCGCGAGCGGCCGCTATGTCATTGCCTACAAC
GGTGAAATTTACAACCACCTGGATTTGCGGGCCGACCTGCAAAAAAGTGGGCAGTCACCGGCCTGGCGTGGGCATTCAGA
TACCGAAACCTTGCTGGCGGGGTTTGATGCCTGGGGCATTCAGGGCGCAGTAGAGCGTACGGTGGGCATGTTTGCCTTTG
CCGTTTGGGACCGCCAGGAACGCATGCTCACGCTGGGACGCGATCGCCTGGGGGAAAAGCCGCTTTACTATGGTTGGCAG
GGCAGTGGCAGCTCTGCTGTCTTCCTGTTCGGTTCAGAGTTGAAGGCCTTGCGTGCGCACCCGGCGTTTGGGGCGCAAAT
CAACCGCGACGCCCTGTGCCTCTACATGCGGCACAACAATGTGGGGGGCTCGCATTCCATTTACCAGGGCATCCACAAGC
TGCCGCCGGGCACGCTGCTCACGGTGTCGCGGCAGACGCCTGAGCCAGTGATTCGGCCCTATTGGTCGGGTGCAGAGGTT
GCCAGGTGCGGCGTGGCACAGCCGTTTGGCGGCACCTCCGGCGAGGCGGTGGATGCGCTGGAGGCCTTGCTGAAAGACGC
GGTGCGTCAGCAAATGATGGCCGATGTGCCGCTGGGCGCCTTTTTGTCCGGCGGGGTGGACTCTTCTGCCGTGGTGGCAC
TGATGCAGACCCAGTCGTTGCGACCGGTCAAGACTTTTTCCATCGGTTTTCACGAGGCGGCCTACAACGAGGCCGAGCAT
GCCAAGGCGGTGGCGCAGCACCTGGGCACCGAGCATACCGAGCTGTATGTGACGCCCGAGCAGGCCATGGCCGTTATTCC
GCGCCTGCCCACGCTGTATGACGAACCGTTTGCCGATTCTTCGCAAATCCCGACCTTCCTGGTCAGCCAGCTGGCGCGTC
AGCACGTCACGGTGTCGCTATCGGGCGATGCCGGCGATGAGCTGTTTGGTGGCTACAACCGTTATCAGCTCACGGCGAGC
CTTTGGCGCAAGCTGTCGGCGGCGCCACTGCCACTACGCCGTGCAGCGGCCTGGGGGTTGACAAGGTTTTCTCCGCAAAG
CCTGAACTCCCTGGCAGCGCTGATGCCAGCCAGCGCCCGCTGGGCCCATATGGGCGACAAGCTGCACAAGGGGGCGGGCG
TGATGGCGGGCCGTACAGTGGAGGACCTGTACCTCGGGATGGTGTCCGACTGGCACGATCCAGCGGCTGTGGTGATCGGC
GGCCGTGAGCCTGCTACCTTGTTGAGGGGGCGGCGGCCAGACTTGCCCGGGCTCACCGATGTGGAGCGCATGATGGCCCT
CGATATGCTGTCCTATTTACCCGATGACATACTCACCAAGGTAGACCGGGCCGCCATGGGTGTCAGCCTGGAAACGCGTG
TGCCGTTTCTGGACCACCGGGTGGTGGAATTCGCCTGGCGACTGCCGCTGCACTACAAGCTGCGCCAGGAAGGCGGGCGC
TACACCACCAAGTGGGCGCTGCGGCAGGTGCTGTACCGCCATGTGCCGCAGGCGTTAATCGACCGTCCCAAGATGGGGTT
TGGCGTGCCGATTGACCGCTGGTTGCGTGGGCCGCTGCGTGACTGGGCGGAAGACTTGCTGAGCGAGACGCGCCTCAAGC
GGGAGGGCTATTTCAAACCTGCTCCCATTCGGCAGAAGTGGGCCGAACATCTGGGCGGGCAGCGCAATTGGGCTCCGCAT
TTATGGACCGCGCTCATGTTCCAGGCCTGGCTCCAGGCTAATCACGCATGA

Upstream 100 bases:

>100_bases
GCCTGGCCGGTCGCCAGTTGGTGGAGCGTTGCTATTGCATTCAGCAAACCGGGCCGCGGATGGCAAAGTTGCTGCATATC
TCAGGTGGGGGGCGCTGAGC

Downstream 100 bases:

>100_bases
CACACACACTGATGTTCGTGGTGAATGTGGATTGGTTTTTCCTGTCTCATCGCTTGCCGATCGCCCTGGAGGCTCAGCGG
CAGGGTTATCAGGTGCACAT

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MCGLAGYFSGQWPADGEQASARLKAMTDAIAHRGPDSDGYWLDAATCMALGHRRLAILELSAAGAQPMHAASGRYVIAYN
GEIYNHLDLRADLQKSGQSPAWRGHSDTETLLAGFDAWGIQGAVERTVGMFAFAVWDRQERMLTLGRDRLGEKPLYYGWQ
GSGSSAVFLFGSELKALRAHPAFGAQINRDALCLYMRHNNVGGSHSIYQGIHKLPPGTLLTVSRQTPEPVIRPYWSGAEV
ARCGVAQPFGGTSGEAVDALEALLKDAVRQQMMADVPLGAFLSGGVDSSAVVALMQTQSLRPVKTFSIGFHEAAYNEAEH
AKAVAQHLGTEHTELYVTPEQAMAVIPRLPTLYDEPFADSSQIPTFLVSQLARQHVTVSLSGDAGDELFGGYNRYQLTAS
LWRKLSAAPLPLRRAAAWGLTRFSPQSLNSLAALMPASARWAHMGDKLHKGAGVMAGRTVEDLYLGMVSDWHDPAAVVIG
GREPATLLRGRRPDLPGLTDVERMMALDMLSYLPDDILTKVDRAAMGVSLETRVPFLDHRVVEFAWRLPLHYKLRQEGGR
YTTKWALRQVLYRHVPQALIDRPKMGFGVPIDRWLRGPLRDWAEDLLSETRLKREGYFKPAPIRQKWAEHLGGQRNWAPH
LWTALMFQAWLQANHA

Sequences:

>Translated_656_residues
MCGLAGYFSGQWPADGEQASARLKAMTDAIAHRGPDSDGYWLDAATCMALGHRRLAILELSAAGAQPMHAASGRYVIAYN
GEIYNHLDLRADLQKSGQSPAWRGHSDTETLLAGFDAWGIQGAVERTVGMFAFAVWDRQERMLTLGRDRLGEKPLYYGWQ
GSGSSAVFLFGSELKALRAHPAFGAQINRDALCLYMRHNNVGGSHSIYQGIHKLPPGTLLTVSRQTPEPVIRPYWSGAEV
ARCGVAQPFGGTSGEAVDALEALLKDAVRQQMMADVPLGAFLSGGVDSSAVVALMQTQSLRPVKTFSIGFHEAAYNEAEH
AKAVAQHLGTEHTELYVTPEQAMAVIPRLPTLYDEPFADSSQIPTFLVSQLARQHVTVSLSGDAGDELFGGYNRYQLTAS
LWRKLSAAPLPLRRAAAWGLTRFSPQSLNSLAALMPASARWAHMGDKLHKGAGVMAGRTVEDLYLGMVSDWHDPAAVVIG
GREPATLLRGRRPDLPGLTDVERMMALDMLSYLPDDILTKVDRAAMGVSLETRVPFLDHRVVEFAWRLPLHYKLRQEGGR
YTTKWALRQVLYRHVPQALIDRPKMGFGVPIDRWLRGPLRDWAEDLLSETRLKREGYFKPAPIRQKWAEHLGGQRNWAPH
LWTALMFQAWLQANHA
>Mature_656_residues
MCGLAGYFSGQWPADGEQASARLKAMTDAIAHRGPDSDGYWLDAATCMALGHRRLAILELSAAGAQPMHAASGRYVIAYN
GEIYNHLDLRADLQKSGQSPAWRGHSDTETLLAGFDAWGIQGAVERTVGMFAFAVWDRQERMLTLGRDRLGEKPLYYGWQ
GSGSSAVFLFGSELKALRAHPAFGAQINRDALCLYMRHNNVGGSHSIYQGIHKLPPGTLLTVSRQTPEPVIRPYWSGAEV
ARCGVAQPFGGTSGEAVDALEALLKDAVRQQMMADVPLGAFLSGGVDSSAVVALMQTQSLRPVKTFSIGFHEAAYNEAEH
AKAVAQHLGTEHTELYVTPEQAMAVIPRLPTLYDEPFADSSQIPTFLVSQLARQHVTVSLSGDAGDELFGGYNRYQLTAS
LWRKLSAAPLPLRRAAAWGLTRFSPQSLNSLAALMPASARWAHMGDKLHKGAGVMAGRTVEDLYLGMVSDWHDPAAVVIG
GREPATLLRGRRPDLPGLTDVERMMALDMLSYLPDDILTKVDRAAMGVSLETRVPFLDHRVVEFAWRLPLHYKLRQEGGR
YTTKWALRQVLYRHVPQALIDRPKMGFGVPIDRWLRGPLRDWAEDLLSETRLKREGYFKPAPIRQKWAEHLGGQRNWAPH
LWTALMFQAWLQANHA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=413, Percent_Identity=29.2978208232446, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI168229252, Length=413, Percent_Identity=29.2978208232446, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI168229250, Length=413, Percent_Identity=29.2978208232446, Blast_Score=124, Evalue=3e-28,
Organism=Homo sapiens, GI296010848, Length=384, Percent_Identity=29.4270833333333, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI296010852, Length=307, Percent_Identity=28.6644951140065, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI296010850, Length=307, Percent_Identity=28.6644951140065, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1786889, Length=532, Percent_Identity=26.8796992481203, Blast_Score=143, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17560178, Length=411, Percent_Identity=28.7104622871046, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71993933, Length=368, Percent_Identity=27.7173913043478, Blast_Score=123, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI25147557, Length=368, Percent_Identity=27.7173913043478, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI25147560, Length=334, Percent_Identity=26.6467065868263, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321563, Length=428, Percent_Identity=27.5700934579439, Blast_Score=121, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6325403, Length=431, Percent_Identity=27.1461716937355, Blast_Score=113, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45553209, Length=372, Percent_Identity=30.1075268817204, Blast_Score=132, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72443; Mature: 72443

Theoretical pI: Translated: 8.54; Mature: 8.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGLAGYFSGQWPADGEQASARLKAMTDAIAHRGPDSDGYWLDAATCMALGHRRLAILEL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCEEEEEEE
SAAGAQPMHAASGRYVIAYNGEIYNHLDLRADLQKSGQSPAWRGHSDTETLLAGFDAWGI
ECCCCCCCCCCCCCEEEEECCCEEECHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHCC
QGAVERTVGMFAFAVWDRQERMLTLGRDRLGEKPLYYGWQGSGSSAVFLFGSELKALRAH
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEECCHHHHHHCC
PAFGAQINRDALCLYMRHNNVGGSHSIYQGIHKLPPGTLLTVSRQTPEPVIRPYWSGAEV
CCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHH
ARCGVAQPFGGTSGEAVDALEALLKDAVRQQMMADVPLGAFLSGGVDSSAVVALMQTQSL
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHCCC
RPVKTFSIGFHEAAYNEAEHAKAVAQHLGTEHTELYVTPEQAMAVIPRLPTLYDEPFADS
CCEEEEECCHHHHHCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCCCCCCCCCCCC
SQIPTFLVSQLARQHVTVSLSGDAGDELFGGYNRYQLTASLWRKLSAAPLPLRRAAAWGL
CCCHHHHHHHHHHHCEEEEECCCCCHHHHCCCCCEEEHHHHHHHHCCCCCCHHHHHHHCC
TRFSPQSLNSLAALMPASARWAHMGDKLHKGAGVMAGRTVEDLYLGMVSDWHDPAAVVIG
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEC
GREPATLLRGRRPDLPGLTDVERMMALDMLSYLPDDILTKVDRAAMGVSLETRVPFLDHR
CCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEECCCCCHHHHH
VVEFAWRLPLHYKLRQEGGRYTTKWALRQVLYRHVPQALIDRPKMGFGVPIDRWLRGPLR
HHHHHHHCCCEEEHHHCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHCCHHH
DWAEDLLSETRLKREGYFKPAPIRQKWAEHLGGQRNWAPHLWTALMFQAWLQANHA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MCGLAGYFSGQWPADGEQASARLKAMTDAIAHRGPDSDGYWLDAATCMALGHRRLAILEL
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCEEEEEEE
SAAGAQPMHAASGRYVIAYNGEIYNHLDLRADLQKSGQSPAWRGHSDTETLLAGFDAWGI
ECCCCCCCCCCCCCEEEEECCCEEECHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCHHCC
QGAVERTVGMFAFAVWDRQERMLTLGRDRLGEKPLYYGWQGSGSSAVFLFGSELKALRAH
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEECCHHHHHHCC
PAFGAQINRDALCLYMRHNNVGGSHSIYQGIHKLPPGTLLTVSRQTPEPVIRPYWSGAEV
CCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCHH
ARCGVAQPFGGTSGEAVDALEALLKDAVRQQMMADVPLGAFLSGGVDSSAVVALMQTQSL
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHCCC
RPVKTFSIGFHEAAYNEAEHAKAVAQHLGTEHTELYVTPEQAMAVIPRLPTLYDEPFADS
CCEEEEECCHHHHHCCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHCCCCCCCCCCCCCC
SQIPTFLVSQLARQHVTVSLSGDAGDELFGGYNRYQLTASLWRKLSAAPLPLRRAAAWGL
CCCHHHHHHHHHHHCEEEEECCCCCHHHHCCCCCEEEHHHHHHHHCCCCCCHHHHHHHCC
TRFSPQSLNSLAALMPASARWAHMGDKLHKGAGVMAGRTVEDLYLGMVSDWHDPAAVVIG
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEC
GREPATLLRGRRPDLPGLTDVERMMALDMLSYLPDDILTKVDRAAMGVSLETRVPFLDHR
CCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCEECCCCCHHHHH
VVEFAWRLPLHYKLRQEGGRYTTKWALRQVLYRHVPQALIDRPKMGFGVPIDRWLRGPLR
HHHHHHHCCCEEEHHHCCCCCHHHHHHHHHHHHHCCHHHHCCCCCCCCCCHHHHHCCHHH
DWAEDLLSETRLKREGYFKPAPIRQKWAEHLGGQRNWAPHLWTALMFQAWLQANHA
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]