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The map label for this gene is potB [H]

Identifier: 91789786

GI number: 91789786

Start: 4135699

End: 4136577

Strand: Reverse

Name: potB [H]

Synonym: Bpro_3946

Alternate gene names: 91789786

Gene position: 4136577-4135699 (Counterclockwise)

Preceding gene: 91789787

Following gene: 91789785

Centisome position: 79.55

GC content: 65.3

Gene sequence:

>879_bases
ATGGTGGGAGCTGCACCGAATGTTTCGCGGCCCTCGGCGGCCCGCGCACCATGGCTGCTGTCGGCGCCGGCCTTTGTGCT
GTTTGCCTGCCTGCTGCTGGTGCCCCTGGCGCTCACGGCCGTGCTGTCCTTCCAGGTGTTCGACCACGCCACCGGCGTCA
AGAACAGTTTCACGCTGGCGCATTACGCCGCGGTGCTGACGGACGACTACTACCACCGCATTTTCTGGCGCACCTTCTGG
GTCTCGGCGCTCACCACGCTGATCTGCGTGCTGGTCGGCGCGCCCGAGGCCTATGTGCTCAGCCGCATGCGCAACCCGTG
GCGCTCTGTCTTGCTGCTGGTGGTGCTGGCGCCACTGCTGGTGTCGGTGGTGGTGCGCGCATTTGGCTGGAGCATGCTGC
TCGGGCCCGAGGGCGTGGTGAACCAGGCCGTGCGCGCCATGGGCCTGCCGCCCATGCGCATCCTCTACACCGAAGCCGCC
GTGGTCATTGCGCTGGTGCATGTGATGCTGCCCTTCATGGTGATCCCGGTCTGGACCTCGCTGCAAAAACTAGACCCCGG
CGTGGAAGATGCCGCGCTGTCGCTCAAGGCCTCGCATTTCACCACCGTACGCCGCATTATTTTTCCGCAGGTGCTGCCCG
GCGTTCTTTCGGGCAGCCTGATCGTCTTCGGCCTGAGCGCCAGCGCCTTTGCCATTCCCGGCCTGCTGGGCGGGCGGCGC
CTGAAGATGGTGGCCACCGTGGTGTACGACGAGTACCTGCACGAGCTCAACTGGCCGCTGGGCGCGGCGATTGCGCTCAC
GCTGCTGCTGGCCAACCTGGTGCTGATGCTCAGCTACAACCGGGTGCTCGAGCGCTCCTACAAAAAATCAATGGGTTAA

Upstream 100 bases:

>100_bases
TCGCGCAGGCCGCTGGCCTTATAGCCGCCCAGGCCCTCACCCCTGTCCTCTCCCGGAGGGAGAGGGAGCAACACGGGGGC
AAGCAGTGAACGCAACAACC

Downstream 100 bases:

>100_bases
GCGGGGTCTGCACCATGTCAAAAAACGGTCCCATCGCACTCATCTTCAACGCCCTCGTCATCGCCTTCATGATGGCGCCG
CTCGTCATCGTTTGCGTGGT

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MVGAAPNVSRPSAARAPWLLSAPAFVLFACLLLVPLALTAVLSFQVFDHATGVKNSFTLAHYAAVLTDDYYHRIFWRTFW
VSALTTLICVLVGAPEAYVLSRMRNPWRSVLLLVVLAPLLVSVVVRAFGWSMLLGPEGVVNQAVRAMGLPPMRILYTEAA
VVIALVHVMLPFMVIPVWTSLQKLDPGVEDAALSLKASHFTTVRRIIFPQVLPGVLSGSLIVFGLSASAFAIPGLLGGRR
LKMVATVVYDEYLHELNWPLGAAIALTLLLANLVLMLSYNRVLERSYKKSMG

Sequences:

>Translated_292_residues
MVGAAPNVSRPSAARAPWLLSAPAFVLFACLLLVPLALTAVLSFQVFDHATGVKNSFTLAHYAAVLTDDYYHRIFWRTFW
VSALTTLICVLVGAPEAYVLSRMRNPWRSVLLLVVLAPLLVSVVVRAFGWSMLLGPEGVVNQAVRAMGLPPMRILYTEAA
VVIALVHVMLPFMVIPVWTSLQKLDPGVEDAALSLKASHFTTVRRIIFPQVLPGVLSGSLIVFGLSASAFAIPGLLGGRR
LKMVATVVYDEYLHELNWPLGAAIALTLLLANLVLMLSYNRVLERSYKKSMG
>Mature_292_residues
MVGAAPNVSRPSAARAPWLLSAPAFVLFACLLLVPLALTAVLSFQVFDHATGVKNSFTLAHYAAVLTDDYYHRIFWRTFW
VSALTTLICVLVGAPEAYVLSRMRNPWRSVLLLVVLAPLLVSVVVRAFGWSMLLGPEGVVNQAVRAMGLPPMRILYTEAA
VVIALVHVMLPFMVIPVWTSLQKLDPGVEDAALSLKASHFTTVRRIIFPQVLPGVLSGSLIVFGLSASAFAIPGLLGGRR
LKMVATVVYDEYLHELNWPLGAAIALTLLLANLVLMLSYNRVLERSYKKSMG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=249, Percent_Identity=32.9317269076305, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1787080, Length=233, Percent_Identity=30.9012875536481, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787713, Length=239, Percent_Identity=26.3598326359833, Blast_Score=87, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31867; Mature: 31867

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVGAAPNVSRPSAARAPWLLSAPAFVLFACLLLVPLALTAVLSFQVFDHATGVKNSFTLA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
HYAAVLTDDYYHRIFWRTFWVSALTTLICVLVGAPEAYVLSRMRNPWRSVLLLVVLAPLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHH
VSVVVRAFGWSMLLGPEGVVNQAVRAMGLPPMRILYTEAAVVIALVHVMLPFMVIPVWTS
HHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQKLDPGVEDAALSLKASHFTTVRRIIFPQVLPGVLSGSLIVFGLSASAFAIPGLLGGRR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHHCCHH
LKMVATVVYDEYLHELNWPLGAAIALTLLLANLVLMLSYNRVLERSYKKSMG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVGAAPNVSRPSAARAPWLLSAPAFVLFACLLLVPLALTAVLSFQVFDHATGVKNSFTLA
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
HYAAVLTDDYYHRIFWRTFWVSALTTLICVLVGAPEAYVLSRMRNPWRSVLLLVVLAPLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHH
VSVVVRAFGWSMLLGPEGVVNQAVRAMGLPPMRILYTEAAVVIALVHVMLPFMVIPVWTS
HHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQKLDPGVEDAALSLKASHFTTVRRIIFPQVLPGVLSGSLIVFGLSASAFAIPGLLGGRR
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHHCCHH
LKMVATVVYDEYLHELNWPLGAAIALTLLLANLVLMLSYNRVLERSYKKSMG
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]