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The map label for this gene is vfr [H]

Identifier: 91789644

GI number: 91789644

Start: 3987803

End: 3988480

Strand: Reverse

Name: vfr [H]

Synonym: Bpro_3800

Alternate gene names: 91789644

Gene position: 3988480-3987803 (Counterclockwise)

Preceding gene: 91789648

Following gene: 91789643

Centisome position: 76.7

GC content: 59.88

Gene sequence:

>678_bases
ATGACGACGATGGTGTCCAATCTGGAATTGATTCGCCGGGTGCCTCTTTTTTCGATGCTGACGGCCAGCCAGGCAGAGTC
CGTGGCCAATGCGGTGGTCAAGCGCCGTTTCAAGCGTGGCGAAGCCATTGTGGAGCAGGGCAAAAAATCAAATGCCCTAT
CTATCATTCTCACCGGGCGTGCACGTGTCATCACCACCGACACCCGGGGCCGCGAGGTCATTCTGGCCACCATGCATCCC
GGCGACTATGTCGGCGAGATGAGCCTGATCGACAATGAACCCCATTCAGCCACGGTATGTGCAGAGGTTCAGACCGACGC
CCTGATACTCGGCCAGCCGGAGTTTGCGCGTTGCCTTCCCGAGAACAGCTCCATGGCTTATGCGGTGCTGAAAGGGCTGG
TGCAGCGCCTGCGCCATGCAGACCGCAAGATTGAATCTCTGGCACTGATGGACGTCTACGGGCGCGTGGCCCGGGCGCTG
CTGGAGTTTGCCAGCGAGGACGGCGAAGGCAATGCCGTGATTCGCGACAAGGTCTCGCGTCAGGATCTGGCCAAGATGGT
CGGCGCTTCACGCGAGATGGTCAGCCGGGTGATGAAAGACCTCGAGGAGCGCGGATTCATTGAAACGCGCGATGACAGTT
CGCTCCTGGTGAGGGAGCGCCTTCATTCGCTGGGTTGA

Upstream 100 bases:

>100_bases
CAGCAGGTAAAAGCTTGGCGGTATTTGTTTTTCTCACGATAAAAGCCATTGTAAGAACTCGCGCGACTTGTCGTCGCCGC
AAGGTTTGGAGAGAGTTGAT

Downstream 100 bases:

>100_bases
CACCGCCTCGCTGTATCCGTTGCACGTCCGCCTCCGCCGAAGTGCTCGCCAACTGCGGTAAGCTCTGACCGGTCCATGAC
TTACTCACTTGACACCCTGA

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 224

Protein sequence:

>225_residues
MTTMVSNLELIRRVPLFSMLTASQAESVANAVVKRRFKRGEAIVEQGKKSNALSIILTGRARVITTDTRGREVILATMHP
GDYVGEMSLIDNEPHSATVCAEVQTDALILGQPEFARCLPENSSMAYAVLKGLVQRLRHADRKIESLALMDVYGRVARAL
LEFASEDGEGNAVIRDKVSRQDLAKMVGASREMVSRVMKDLEERGFIETRDDSSLLVRERLHSLG

Sequences:

>Translated_225_residues
MTTMVSNLELIRRVPLFSMLTASQAESVANAVVKRRFKRGEAIVEQGKKSNALSIILTGRARVITTDTRGREVILATMHP
GDYVGEMSLIDNEPHSATVCAEVQTDALILGQPEFARCLPENSSMAYAVLKGLVQRLRHADRKIESLALMDVYGRVARAL
LEFASEDGEGNAVIRDKVSRQDLAKMVGASREMVSRVMKDLEERGFIETRDDSSLLVRERLHSLG
>Mature_224_residues
TTMVSNLELIRRVPLFSMLTASQAESVANAVVKRRFKRGEAIVEQGKKSNALSIILTGRARVITTDTRGREVILATMHPG
DYVGEMSLIDNEPHSATVCAEVQTDALILGQPEFARCLPENSSMAYAVLKGLVQRLRHADRKIESLALMDVYGRVARALL
EFASEDGEGNAVIRDKVSRQDLAKMVGASREMVSRVMKDLEERGFIETRDDSSLLVRERLHSLG

Specific function: Can bind cyclic AMP. Is a global regulator of virulence factor expression and is required for exotoxin A and protease production [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=181, Percent_Identity=28.7292817679558, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24914; Mature: 24783

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS50042 CNMP_BINDING_3 ; PS00042 HTH_CRP_1 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTMVSNLELIRRVPLFSMLTASQAESVANAVVKRRFKRGEAIVEQGKKSNALSIILTGR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
ARVITTDTRGREVILATMHPGDYVGEMSLIDNEPHSATVCAEVQTDALILGQPEFARCLP
EEEEEECCCCCEEEEEEECCCCHHCCHHHCCCCCCCCEEEEECCCCEEEECCCHHHHHCC
ENSSMAYAVLKGLVQRLRHADRKIESLALMDVYGRVARALLEFASEDGEGNAVIRDKVSR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
QDLAKMVGASREMVSRVMKDLEERGFIETRDDSSLLVRERLHSLG
HHHHHHHCCCHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TTMVSNLELIRRVPLFSMLTASQAESVANAVVKRRFKRGEAIVEQGKKSNALSIILTGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
ARVITTDTRGREVILATMHPGDYVGEMSLIDNEPHSATVCAEVQTDALILGQPEFARCLP
EEEEEECCCCCEEEEEEECCCCHHCCHHHCCCCCCCCEEEEECCCCEEEECCCHHHHHCC
ENSSMAYAVLKGLVQRLRHADRKIESLALMDVYGRVARALLEFASEDGEGNAVIRDKVSR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
QDLAKMVGASREMVSRVMKDLEERGFIETRDDSSLLVRERLHSLG
HHHHHHHCCCHHHHHHHHHHHHHCCCEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8002577; 10984043 [H]