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The map label for this gene is xthA [H]

Identifier: 91789279

GI number: 91789279

Start: 3621747

End: 3622565

Strand: Direct

Name: xthA [H]

Synonym: Bpro_3424

Alternate gene names: 91789279

Gene position: 3621747-3622565 (Clockwise)

Preceding gene: 91789278

Following gene: 91789286

Centisome position: 69.65

GC content: 62.52

Gene sequence:

>819_bases
ATGAAGCTGGCCACCTTCAACGTCAACGGCATCAAGACCCGGCTGCCCAACCTGCTGTCGTGGCTGGCGAAGGAGTCGCC
AGACCTTGTCTGCCTGCAGGAACTCAAGGCGCTGGACGGCGTGTTTCCCCAGGCTGAATTGCGCGAGGCCGGCTACGGTG
CCGTGTGGAAAGGCCAGCGCTCCTGGAACGGCGTGGCAATTCTGGCCAAGGACAGCGATCCGGTCGAGATCAGGCGGGAA
TTGCCGGGCGACCCTGGCGACGACCAGAGCCGCTACCTGGAGGCCACGGTGAACGGCATCATCGTGGCCTGCCTCTACCT
GCCCAATGGCAACCCGCAACCGGGACCCAAGTTTGACTACAAGCTCGCCTGGTTCGAGCGTCTGAACCGGCACGCACAGG
CGCTGTACCAGTCCGGCCAACCGGTGGCGCTGGCGGGGGACTTCAATGTGGTGCCCACCGACTTCGACATCTACAACCCG
AAGTCGTGGCTGAAGGACGCGCTGCTGCAGCCCGAAAGCCGCGAGGCCTATGCCAGACTGCTGTCGCAGGGCTGGGTCGA
CAGCCTGCGCCACCTGCACCCCGACGAGCGCATCTACACCTTCTGGGATTATTTTCGCCAGCACTGGCAAAAAAACTCGG
GCCTGCGTATCGACCACATTTTGCTCAATGCAGAACTGGCGCCAGGACTGAAGGCTGCCGGTGTGGACACGTGGGTACGC
GATGAAGAACACGCCAGCGACCATGCGCCCACATGGGTGGAGCTGGCAGATTCGGAGGTGGCGAAAATCGCCCGAAAACA
AGGTTCACAACCCCGATGA

Upstream 100 bases:

>100_bases
CAAGCCGGTGCCGCATGATTTTCCCGATGGTGGCGGCAGCGGCGGCGCGGAAGCCTACCGCCGCAAGAAAACTGAACGCT
GAACGGCGCCCGCTGCTCCC

Downstream 100 bases:

>100_bases
CAAGGCGGCCGGGCCACCGGCAGCCGAGACCGGCAGCACGATGCGCAACGCTCAGGCAGGCAGGAGTTCAGCGCGCTCCA
GCAAGTCAGCGCTGCGCCGG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKLATFNVNGIKTRLPNLLSWLAKESPDLVCLQELKALDGVFPQAELREAGYGAVWKGQRSWNGVAILAKDSDPVEIRRE
LPGDPGDDQSRYLEATVNGIIVACLYLPNGNPQPGPKFDYKLAWFERLNRHAQALYQSGQPVALAGDFNVVPTDFDIYNP
KSWLKDALLQPESREAYARLLSQGWVDSLRHLHPDERIYTFWDYFRQHWQKNSGLRIDHILLNAELAPGLKAAGVDTWVR
DEEHASDHAPTWVELADSEVAKIARKQGSQPR

Sequences:

>Translated_272_residues
MKLATFNVNGIKTRLPNLLSWLAKESPDLVCLQELKALDGVFPQAELREAGYGAVWKGQRSWNGVAILAKDSDPVEIRRE
LPGDPGDDQSRYLEATVNGIIVACLYLPNGNPQPGPKFDYKLAWFERLNRHAQALYQSGQPVALAGDFNVVPTDFDIYNP
KSWLKDALLQPESREAYARLLSQGWVDSLRHLHPDERIYTFWDYFRQHWQKNSGLRIDHILLNAELAPGLKAAGVDTWVR
DEEHASDHAPTWVELADSEVAKIARKQGSQPR
>Mature_272_residues
MKLATFNVNGIKTRLPNLLSWLAKESPDLVCLQELKALDGVFPQAELREAGYGAVWKGQRSWNGVAILAKDSDPVEIRRE
LPGDPGDDQSRYLEATVNGIIVACLYLPNGNPQPGPKFDYKLAWFERLNRHAQALYQSGQPVALAGDFNVVPTDFDIYNP
KSWLKDALLQPESREAYARLLSQGWVDSLRHLHPDERIYTFWDYFRQHWQKNSGLRIDHILLNAELAPGLKAAGVDTWVR
DEEHASDHAPTWVELADSEVAKIARKQGSQPR

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=264, Percent_Identity=30.3030303030303, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI18375503, Length=264, Percent_Identity=30.3030303030303, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI18375501, Length=264, Percent_Identity=30.3030303030303, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1788046, Length=264, Percent_Identity=35.6060606060606, Blast_Score=159, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI71989536, Length=268, Percent_Identity=27.2388059701493, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI221330655, Length=266, Percent_Identity=29.3233082706767, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17136678, Length=266, Percent_Identity=29.3233082706767, Blast_Score=108, Evalue=3e-24,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 30764; Mature: 30764

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLATFNVNGIKTRLPNLLSWLAKESPDLVCLQELKALDGVFPQAELREAGYGAVWKGQR
CEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCEECCCC
SWNGVAILAKDSDPVEIRRELPGDPGDDQSRYLEATVNGIIVACLYLPNGNPQPGPKFDY
CCCCEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCH
KLAWFERLNRHAQALYQSGQPVALAGDFNVVPTDFDIYNPKSWLKDALLQPESREAYARL
HHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHH
LSQGWVDSLRHLHPDERIYTFWDYFRQHWQKNSGLRIDHILLNAELAPGLKAAGVDTWVR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHC
DEEHASDHAPTWVELADSEVAKIARKQGSQPR
CCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLATFNVNGIKTRLPNLLSWLAKESPDLVCLQELKALDGVFPQAELREAGYGAVWKGQR
CEEEEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHCCCCCEECCCC
SWNGVAILAKDSDPVEIRRELPGDPGDDQSRYLEATVNGIIVACLYLPNGNPQPGPKFDY
CCCCEEEEECCCCHHHHHHHCCCCCCCCHHHHHHHHHCEEEEEEEECCCCCCCCCCCCCH
KLAWFERLNRHAQALYQSGQPVALAGDFNVVPTDFDIYNPKSWLKDALLQPESREAYARL
HHHHHHHHHHHHHHHHHCCCCEEEECCCEECCCCCCCCCCHHHHHHHHCCCCCHHHHHHH
LSQGWVDSLRHLHPDERIYTFWDYFRQHWQKNSGLRIDHILLNAELAPGLKAAGVDTWVR
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHHC
DEEHASDHAPTWVELADSEVAKIARKQGSQPR
CCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]