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The map label for this gene is gatA [H]

Identifier: 91789187

GI number: 91789187

Start: 3525212

End: 3526498

Strand: Reverse

Name: gatA [H]

Synonym: Bpro_3331

Alternate gene names: 91789187

Gene position: 3526498-3525212 (Counterclockwise)

Preceding gene: 91789188

Following gene: 91789186

Centisome position: 67.81

GC content: 64.1

Gene sequence:

>1287_bases
ATGCATAACATTCTGACGCTGTCTGCGCGGGACCTCGCCGCCAGGGTTTCGCGCGGGGAATTGACGGCTGAAGCCGTGAC
GCGGGCCTACATCGAACAGATCCAGTCTTGCGAACTTTCGGTCAAGGCATGGCAGTTCTTTGACGCCGACATGGCGCTGC
AAAGCGCGCGTGCACTGGACCGCCACAGCCACAGCAGCAACGCACTGCTGCGAGGCGTCCCCGTCGGGGTGAAAGACCTG
ATGGATACGGTCGACATGCCAACCTCCTATGGTTCGCCTATTTATGCGGGGCACCGTCCGAAGTTCGATGCCGCATGCGT
GGCCGCCTGCCGGGATGCCGGGATGGTGGTGTTGGGCAAAACTGTTACCACCGAGTTTGCAACCTTTCAGCCTGGAGTGA
CATGCAATCCCCGCGCGCCAGCAGATGCGCCGCGCACGCCGGGCGGATCTTCCAGCGGGTCCGCCGCGGCGGTGGCTGCG
AACATGGTGCCACTGGCACTCGGAACCCAGACGGCTGGCTCCATCGTGCGGCCGGCGGCCTACTGTGGCGTGATTGGCTA
CAAGCCCACCCACGGGACATTGCCGCTGGCCGGCGTCAAGCCGCTCGCGCCCAGTCTCGACACGATAGGCGCGTTCGCAC
GCTCGGTGGATGATGCCGCATTTTTTGTTGGCGCGTTGACCCGTGTCAAACTGGACCCGCAGCGGCAATCTGCCCTTCGC
GTGGGCATTTGCCGTACCGCCCATTGGGATCTGGCCTCGGACGCTACACGCCGCGTGCTTGAGCATTCCGCACGCCAGTT
CGAAAAGGCTGGAGCACATGTTCATGACCTTGTTTTGCCGGCTTCGTGCGATGCCTTGACGCAGGCGCAAATTCAGATAA
TGGCTTATGAAGCCGCGGCTGCGTTTGCGCCGGAGCGTCAGCGGCACGCGGCACAGTTCAGCCCGGCCTTTGCGCAACTC
CTGGCGTTGGGACAGTCGCTGCACGGGGCCGACTTCGCCGCTGCGCAGTTGCTGGCTATTGCTGCGCGACGGGCGCTGGA
CACCGTGTTCGAGTCGGTGGATGTGCTGCTGGCTCCCAGCGCCGAAGGCGAGGCGCCTGCAGGCTTGGGCGCCACGGGTA
ATCCCATCTTCAACCGTCTCTGGACGCTGCTGGGGAATCCCTGTGTGCATGTGCCGGCCGGGACGGGGGAGCACGGTATG
CCCGTAGGCGTCACGGTCATAGGCCCGCGATGGGCTGATGCGCTGACATTGAGCGCAGCGCATACGCTGGAGCTCAGTCT
GAGCTGA

Upstream 100 bases:

>100_bases
ATATGTTCTTTCGCAGGGCAAGGTCGTCGCCTCCGGTTCGGCACAAGCGCTGTCCGAGAACGACGAAGTGCGTTCCGCAT
ATTTCGGCTGAGTCAGTGAT

Downstream 100 bases:

>100_bases
ACATGGATAAAGAATTGAATGTGACTAAACCAGAAAAACGAGACCAGACGACGGCCTGCGAACGTGCGCAGGACCATGTG
CAACATGATCGCCTGCTGTC

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MHNILTLSARDLAARVSRGELTAEAVTRAYIEQIQSCELSVKAWQFFDADMALQSARALDRHSHSSNALLRGVPVGVKDL
MDTVDMPTSYGSPIYAGHRPKFDAACVAACRDAGMVVLGKTVTTEFATFQPGVTCNPRAPADAPRTPGGSSSGSAAAVAA
NMVPLALGTQTAGSIVRPAAYCGVIGYKPTHGTLPLAGVKPLAPSLDTIGAFARSVDDAAFFVGALTRVKLDPQRQSALR
VGICRTAHWDLASDATRRVLEHSARQFEKAGAHVHDLVLPASCDALTQAQIQIMAYEAAAAFAPERQRHAAQFSPAFAQL
LALGQSLHGADFAAAQLLAIAARRALDTVFESVDVLLAPSAEGEAPAGLGATGNPIFNRLWTLLGNPCVHVPAGTGEHGM
PVGVTVIGPRWADALTLSAAHTLELSLS

Sequences:

>Translated_428_residues
MHNILTLSARDLAARVSRGELTAEAVTRAYIEQIQSCELSVKAWQFFDADMALQSARALDRHSHSSNALLRGVPVGVKDL
MDTVDMPTSYGSPIYAGHRPKFDAACVAACRDAGMVVLGKTVTTEFATFQPGVTCNPRAPADAPRTPGGSSSGSAAAVAA
NMVPLALGTQTAGSIVRPAAYCGVIGYKPTHGTLPLAGVKPLAPSLDTIGAFARSVDDAAFFVGALTRVKLDPQRQSALR
VGICRTAHWDLASDATRRVLEHSARQFEKAGAHVHDLVLPASCDALTQAQIQIMAYEAAAAFAPERQRHAAQFSPAFAQL
LALGQSLHGADFAAAQLLAIAARRALDTVFESVDVLLAPSAEGEAPAGLGATGNPIFNRLWTLLGNPCVHVPAGTGEHGM
PVGVTVIGPRWADALTLSAAHTLELSLS
>Mature_428_residues
MHNILTLSARDLAARVSRGELTAEAVTRAYIEQIQSCELSVKAWQFFDADMALQSARALDRHSHSSNALLRGVPVGVKDL
MDTVDMPTSYGSPIYAGHRPKFDAACVAACRDAGMVVLGKTVTTEFATFQPGVTCNPRAPADAPRTPGGSSSGSAAAVAA
NMVPLALGTQTAGSIVRPAAYCGVIGYKPTHGTLPLAGVKPLAPSLDTIGAFARSVDDAAFFVGALTRVKLDPQRQSALR
VGICRTAHWDLASDATRRVLEHSARQFEKAGAHVHDLVLPASCDALTQAQIQIMAYEAAAAFAPERQRHAAQFSPAFAQL
LALGQSLHGADFAAAQLLAIAARRALDTVFESVDVLLAPSAEGEAPAGLGATGNPIFNRLWTLLGNPCVHVPAGTGEHGM
PVGVTVIGPRWADALTLSAAHTLELSLS

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=505, Percent_Identity=24.7524752475248, Blast_Score=113, Evalue=4e-25,
Organism=Homo sapiens, GI195972892, Length=480, Percent_Identity=24.5833333333333, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=419, Percent_Identity=27.4463007159904, Blast_Score=107, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17537465, Length=495, Percent_Identity=22.6262626262626, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319685, Length=387, Percent_Identity=27.390180878553, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323950, Length=427, Percent_Identity=24.824355971897, Blast_Score=95, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21356731, Length=472, Percent_Identity=26.0593220338983, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24648113, Length=492, Percent_Identity=26.8292682926829, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24644968, Length=478, Percent_Identity=23.4309623430962, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648435, Length=470, Percent_Identity=22.3404255319149, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24648437, Length=470, Percent_Identity=22.3404255319149, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45550774, Length=470, Percent_Identity=22.3404255319149, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI161078093, Length=478, Percent_Identity=23.6401673640167, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 44705; Mature: 44705

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: PS00435 PEROXIDASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNILTLSARDLAARVSRGELTAEAVTRAYIEQIQSCELSVKAWQFFDADMALQSARALD
CCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHH
RHSHSSNALLRGVPVGVKDLMDTVDMPTSYGSPIYAGHRPKFDAACVAACRDAGMVVLGK
HCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECC
TVTTEFATFQPGVTCNPRAPADAPRTPGGSSSGSAAAVAANMVPLALGTQTAGSIVRPAA
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEEECCCCCCCHHCHHH
YCGVIGYKPTHGTLPLAGVKPLAPSLDTIGAFARSVDDAAFFVGALTRVKLDPQRQSALR
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHH
VGICRTAHWDLASDATRRVLEHSARQFEKAGAHVHDLVLPASCDALTQAQIQIMAYEAAA
HHEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHEEEEEEHHHH
AFAPERQRHAAQFSPAFAQLLALGQSLHGADFAAAQLLAIAARRALDTVFESVDVLLAPS
HCCCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEECC
AEGEAPAGLGATGNPIFNRLWTLLGNPCVHVPAGTGEHGMPVGVTVIGPRWADALTLSAA
CCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEECCCCCCCEEEEEE
HTLELSLS
EEEEEEEC
>Mature Secondary Structure
MHNILTLSARDLAARVSRGELTAEAVTRAYIEQIQSCELSVKAWQFFDADMALQSARALD
CCCEEEECHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHH
RHSHSSNALLRGVPVGVKDLMDTVDMPTSYGSPIYAGHRPKFDAACVAACRDAGMVVLGK
HCCCCCCCEEECCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEECC
TVTTEFATFQPGVTCNPRAPADAPRTPGGSSSGSAAAVAANMVPLALGTQTAGSIVRPAA
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHEEEECCCCCCCHHCHHH
YCGVIGYKPTHGTLPLAGVKPLAPSLDTIGAFARSVDDAAFFVGALTRVKLDPQRQSALR
HHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHH
VGICRTAHWDLASDATRRVLEHSARQFEKAGAHVHDLVLPASCDALTQAQIQIMAYEAAA
HHEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHEEEEEEHHHH
AFAPERQRHAAQFSPAFAQLLALGQSLHGADFAAAQLLAIAARRALDTVFESVDVLLAPS
HCCCHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEECC
AEGEAPAGLGATGNPIFNRLWTLLGNPCVHVPAGTGEHGMPVGVTVIGPRWADALTLSAA
CCCCCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEECCCCCCCEEEEEE
HTLELSLS
EEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA