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The map label for this gene is puuR [H]

Identifier: 91789185

GI number: 91789185

Start: 3523278

End: 3523883

Strand: Reverse

Name: puuR [H]

Synonym: Bpro_3329

Alternate gene names: 91789185

Gene position: 3523883-3523278 (Counterclockwise)

Preceding gene: 91789186

Following gene: 91789181

Centisome position: 67.76

GC content: 56.93

Gene sequence:

>606_bases
ATGGAGATGCTCGTGAAATCAAATATCGTTGCTGCGCCGACCCCGGAAGACGCTGCCGTCAAACCACCCTCCATGCTGGG
TGTCCGGTTGAGGCATGCCCGCCTGGTTGCCGGTCTGACCATGACGCAGGTGGCTCAGAAGTCGCAATGTTCAGAAAGCC
TGATCTCAAAAATTGAAAGCGGACAGGCCTCTCCTTCACTGGCCATGCTGCATCGAATTGCGGTCACGCTGGACACAAAC
ATTGCTGCTTTGACGACGGAAGAAGCGCCCAGGGAGGGTCCCATCCGGCTCAGTGGCGAGCGCCCCGTGATCAAGGCCGG
GGAAATTTCCCTCGAGCGCATCACGCTTCCCAAGCGGGGTGGCCTGTTGCAGGCCAATATTCACATCGTTCCGCCAGGCG
AAGCGAGCGATGGCTTGATAGAGCATGTAGGGGAAGAAGTTGGCTTTGTTCTGGAGGGCAGCCTTGAGCTGGTACTGGGG
AGTGAGCGTCACCTGATCCAGGCTGGGGACGCGTTTTATTTTTCCAGTCAGACGCCTCATGGGTATCGCAATGTCGGCAA
GGTCGCTGCAAGAATCTTTTGGGTGAATACGCCAGCGACTTTCTAA

Upstream 100 bases:

>100_bases
TGCTGCGGAGGAAACCGCGCCTCATGAGCTGTTTCTGGGTGTTCAGGCGCTGGCTTATGCTGTGACGTCGCTGGCCAATG
CCTGACCCTCGGGAAAACAG

Downstream 100 bases:

>100_bases
CCTGGCGCCTGCACCGCAACGATCCGCATCAAGCTGCTCAAGATCGGCGCAGCCATCGTGCGCAATACCCGCCGTGTGCG
GGTGCTGCTGGCTTCACAAC

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MEMLVKSNIVAAPTPEDAAVKPPSMLGVRLRHARLVAGLTMTQVAQKSQCSESLISKIESGQASPSLAMLHRIAVTLDTN
IAALTTEEAPREGPIRLSGERPVIKAGEISLERITLPKRGGLLQANIHIVPPGEASDGLIEHVGEEVGFVLEGSLELVLG
SERHLIQAGDAFYFSSQTPHGYRNVGKVAARIFWVNTPATF

Sequences:

>Translated_201_residues
MEMLVKSNIVAAPTPEDAAVKPPSMLGVRLRHARLVAGLTMTQVAQKSQCSESLISKIESGQASPSLAMLHRIAVTLDTN
IAALTTEEAPREGPIRLSGERPVIKAGEISLERITLPKRGGLLQANIHIVPPGEASDGLIEHVGEEVGFVLEGSLELVLG
SERHLIQAGDAFYFSSQTPHGYRNVGKVAARIFWVNTPATF
>Mature_201_residues
MEMLVKSNIVAAPTPEDAAVKPPSMLGVRLRHARLVAGLTMTQVAQKSQCSESLISKIESGQASPSLAMLHRIAVTLDTN
IAALTTEEAPREGPIRLSGERPVIKAGEISLERITLPKRGGLLQANIHIVPPGEASDGLIEHVGEEVGFVLEGSLELVLG
SERHLIQAGDAFYFSSQTPHGYRNVGKVAARIFWVNTPATF

Specific function: Represses puuD [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=177, Percent_Identity=25.9887005649718, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 21520; Mature: 21520

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMLVKSNIVAAPTPEDAAVKPPSMLGVRLRHARLVAGLTMTQVAQKSQCSESLISKIES
CCCEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
GQASPSLAMLHRIAVTLDTNIAALTTEEAPREGPIRLSGERPVIKAGEISLERITLPKRG
CCCCCHHHHHHHEEEEECCCEEEEECCCCCCCCCEEECCCCCEEEECCEEEEEEECCCCC
GLLQANIHIVPPGEASDGLIEHVGEEVGFVLEGSLELVLGSERHLIQAGDAFYFSSQTPH
CEEEEEEEEECCCCCCCHHHHHHHHHCCEEEECCEEEEECCCCEEEEECCEEEECCCCCC
GYRNVGKVAARIFWVNTPATF
CHHHHHHHEEEEEEECCCCCC
>Mature Secondary Structure
MEMLVKSNIVAAPTPEDAAVKPPSMLGVRLRHARLVAGLTMTQVAQKSQCSESLISKIES
CCCEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHC
GQASPSLAMLHRIAVTLDTNIAALTTEEAPREGPIRLSGERPVIKAGEISLERITLPKRG
CCCCCHHHHHHHEEEEECCCEEEEECCCCCCCCCEEECCCCCEEEECCEEEEEEECCCCC
GLLQANIHIVPPGEASDGLIEHVGEEVGFVLEGSLELVLGSERHLIQAGDAFYFSSQTPH
CEEEEEEEEECCCCCCCHHHHHHHHHCCEEEECCEEEEECCCCEEEEECCEEEECCCCCC
GYRNVGKVAARIFWVNTPATF
CHHHHHHHEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]