Definition Polaromonas sp. JS666 chromosome, complete genome.
Accession NC_007948
Length 5,200,264

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The map label for this gene is pnp

Identifier: 91789120

GI number: 91789120

Start: 3449758

End: 3451881

Strand: Reverse

Name: pnp

Synonym: Bpro_3260

Alternate gene names: 91789120

Gene position: 3451881-3449758 (Counterclockwise)

Preceding gene: 91789121

Following gene: 91789119

Centisome position: 66.38

GC content: 61.72

Gene sequence:

>2124_bases
ATGAGCATTTTTAATAAAGTTACCAAATCCTTCCAGTGGGGCCAGCACAAGGTCACGATGGAAACCGGTGAAGTCGCGCG
CCAGGCCAGCGGTGCCGTGGTTGTCGACATGGACGGCACCGTGGTGCTGGCCACCGTGGTTGCCAAGACCGATGCCAAGC
CCGGCCAGGACTTTTTCCCCCTGACGGTCGATTACCTCGAGAAAACCTACGCAGCCGGCCGCATTCCTGGCAGCTTTTTC
AAGCGTGAAGGCCGCCCCAGCGAATTTGAAACGCTGACGTCGCGCCTGATCGACCGTCCGATTCGTCCGCTCTTCCCCGA
AGGCTTCTTCAACGAAGTGCAGGTCGTGATCCATGTGTTGTCGCTGAACCCTGAAGTCGAGGGCGACATTCCCGCGCTGA
TCGCATCGAGCGCCGCCCTGTCGATTTCCGGCATTCCGTTCAACGGCCCGATTGGCGCCGCCCGCGTGGCCTACGTCGAC
GGCCAGTATGTGTTGAATCCCGGCAAGACCCAGCTGAAAGATTCGAAGATGGATCTGGTCGTGGCCGGCACCGAAGCCGC
CGTGCTGATGGTCGAGTCCGAAGCCCAGCAGCTGTCTGAAGAAATCATGCTCGGCGCCGTGGTGTTTGGTCACGAACAGG
CCAACATCGCCATCAACGCAATTCACGAACTCGTGCGCGACGCCGGCAAGCCGGTCTGGGACTGGCAGGCGCCGGCGAAA
GACGAGCCGCTGATTGCAAAGGTCAACGAACTGGCCGGCGCCAAGCTGCAGGCCGCTTACCAGATCCGCAGCAAGCAGGC
GCGCACCCAGGCTTGCCGCGTGGCTTATGCCGACGTGATGGCCGCCCTGAAGGCGGACGGCGTGGCATTTGACGGCGTGA
CCGTTGAAGGCATGCTGTTCGACATCGAAGCCAAAATCGTCCGCAGCCAGATCCTGGCCGGCGAGCCACGCATTGACGGC
CGCGACACGCGAACCGTGCGTGCCATTGAAATCCGCAACAGCGTGCTGCCCCGCACCCACGGCTCGGCCCTGTTCACGCG
CGGCGAAACCCAGGCGCTGGTCGTCACCACGCTGGGCACCGAGCGCGACGCGCAGCGCATCGACGCACTGTCGGGCGACT
ACGAAGACCGCTTCATGCTGCACTACAACATGCCTCCGTTCGCCACCGGCGAAACCGGCCGTGTCGGCAGCCCGAAACGC
CGCGAGATCGGCCACGGCCGTCTGGCCAAGCGCGCCCTGATTGCCGTGCTGCCCACAAAAGAAGAATTCCCATACACCAT
GCGCGTGGTGTCTGAAATCACCGAATCCAACGGCTCCTCGTCGATGGCTTCGGTCTGCGGCGGCTGCCTGTCGCTGATGG
ACGCCGGCGTTCCGATGAAAGCGCACGTGGCTGGTATCGCCATGGGCCTGATCAAGGAAGAAAACCGTTTCGCCGTGCTG
ACCGACATCCTGGGTGACGAAGATCATCTGGGCGACATGGACTTCAAAGTGGCCGGTACCACCTTCGGTATCACCGCGCT
GCAGATGGACATCAAGATCCAGGGCATCACGAAAGAGATCATGCAGGTTGCGCTGGCCCAGGCCAAGGAAGCCCGCATGC
ACATCCTGGGCAAGATGCAGGAAGCCATGGGTGAAGCCAAGGCCGAGGTGTCTGACTTCGCGCCGCGCCTCTACGTGATG
AAGATCAATCCCGAGAAGATCCGTGACGTGATCGGCAAGGGCGGCGCCGTCATCCGTGCGCTGACCGAAGAAACCGGCAC
GCAGATCAACATCGAGGAAGATGGCACGATCACCATCGCGTCGAACGACAGCGCCAAGGCCGACGAGGCCAAGCGCCGCA
TCGCCGAGATCACCGCGGAAGTCGAAATCGGCAAGGTCTACGAAGGCGCCATCACCAAGATCCTGGACTTCGGTGCACTG
GTCAACCTGCTGCCCGGCAAGGACGGCCTGCTGCACATCAGCCAGATCGCCCACGAGCGTGTCGAGAAGGTCACCGACTA
CCTGAGCGAAGGCCAGATCGTCAAGGTCAAGGTTCTGGAGACCGATGAAAAAGGTCGCGTCAAGCTGTCGATGAAGGCCT
TGCTGGACCGTCCTGCCCAAAATCAGGATCAGGACCGGGGTTAA

Upstream 100 bases:

>100_bases
CCGTGATTCAGGCGTTTTGTGGAATGGCATCGTGTTCAGCCAGCCCCGCTCCGGGCCCCAGGGTGCAGCCTCATGCACCC
GGATCTAGTGGAGCAATAAC

Downstream 100 bases:

>100_bases
TCTTTTAACTGCTATTGATTTTGTAGCCGCTAGTCCCTGAATATGCGGACTGGCGGCGCAAATGATCTGGAATCCCTATG
AAAGCCATTGAAATCACCTC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 707; Mature: 706

Protein sequence:

>707_residues
MSIFNKVTKSFQWGQHKVTMETGEVARQASGAVVVDMDGTVVLATVVAKTDAKPGQDFFPLTVDYLEKTYAAGRIPGSFF
KREGRPSEFETLTSRLIDRPIRPLFPEGFFNEVQVVIHVLSLNPEVEGDIPALIASSAALSISGIPFNGPIGAARVAYVD
GQYVLNPGKTQLKDSKMDLVVAGTEAAVLMVESEAQQLSEEIMLGAVVFGHEQANIAINAIHELVRDAGKPVWDWQAPAK
DEPLIAKVNELAGAKLQAAYQIRSKQARTQACRVAYADVMAALKADGVAFDGVTVEGMLFDIEAKIVRSQILAGEPRIDG
RDTRTVRAIEIRNSVLPRTHGSALFTRGETQALVVTTLGTERDAQRIDALSGDYEDRFMLHYNMPPFATGETGRVGSPKR
REIGHGRLAKRALIAVLPTKEEFPYTMRVVSEITESNGSSSMASVCGGCLSLMDAGVPMKAHVAGIAMGLIKEENRFAVL
TDILGDEDHLGDMDFKVAGTTFGITALQMDIKIQGITKEIMQVALAQAKEARMHILGKMQEAMGEAKAEVSDFAPRLYVM
KINPEKIRDVIGKGGAVIRALTEETGTQINIEEDGTITIASNDSAKADEAKRRIAEITAEVEIGKVYEGAITKILDFGAL
VNLLPGKDGLLHISQIAHERVEKVTDYLSEGQIVKVKVLETDEKGRVKLSMKALLDRPAQNQDQDRG

Sequences:

>Translated_707_residues
MSIFNKVTKSFQWGQHKVTMETGEVARQASGAVVVDMDGTVVLATVVAKTDAKPGQDFFPLTVDYLEKTYAAGRIPGSFF
KREGRPSEFETLTSRLIDRPIRPLFPEGFFNEVQVVIHVLSLNPEVEGDIPALIASSAALSISGIPFNGPIGAARVAYVD
GQYVLNPGKTQLKDSKMDLVVAGTEAAVLMVESEAQQLSEEIMLGAVVFGHEQANIAINAIHELVRDAGKPVWDWQAPAK
DEPLIAKVNELAGAKLQAAYQIRSKQARTQACRVAYADVMAALKADGVAFDGVTVEGMLFDIEAKIVRSQILAGEPRIDG
RDTRTVRAIEIRNSVLPRTHGSALFTRGETQALVVTTLGTERDAQRIDALSGDYEDRFMLHYNMPPFATGETGRVGSPKR
REIGHGRLAKRALIAVLPTKEEFPYTMRVVSEITESNGSSSMASVCGGCLSLMDAGVPMKAHVAGIAMGLIKEENRFAVL
TDILGDEDHLGDMDFKVAGTTFGITALQMDIKIQGITKEIMQVALAQAKEARMHILGKMQEAMGEAKAEVSDFAPRLYVM
KINPEKIRDVIGKGGAVIRALTEETGTQINIEEDGTITIASNDSAKADEAKRRIAEITAEVEIGKVYEGAITKILDFGAL
VNLLPGKDGLLHISQIAHERVEKVTDYLSEGQIVKVKVLETDEKGRVKLSMKALLDRPAQNQDQDRG
>Mature_706_residues
SIFNKVTKSFQWGQHKVTMETGEVARQASGAVVVDMDGTVVLATVVAKTDAKPGQDFFPLTVDYLEKTYAAGRIPGSFFK
REGRPSEFETLTSRLIDRPIRPLFPEGFFNEVQVVIHVLSLNPEVEGDIPALIASSAALSISGIPFNGPIGAARVAYVDG
QYVLNPGKTQLKDSKMDLVVAGTEAAVLMVESEAQQLSEEIMLGAVVFGHEQANIAINAIHELVRDAGKPVWDWQAPAKD
EPLIAKVNELAGAKLQAAYQIRSKQARTQACRVAYADVMAALKADGVAFDGVTVEGMLFDIEAKIVRSQILAGEPRIDGR
DTRTVRAIEIRNSVLPRTHGSALFTRGETQALVVTTLGTERDAQRIDALSGDYEDRFMLHYNMPPFATGETGRVGSPKRR
EIGHGRLAKRALIAVLPTKEEFPYTMRVVSEITESNGSSSMASVCGGCLSLMDAGVPMKAHVAGIAMGLIKEENRFAVLT
DILGDEDHLGDMDFKVAGTTFGITALQMDIKIQGITKEIMQVALAQAKEARMHILGKMQEAMGEAKAEVSDFAPRLYVMK
INPEKIRDVIGKGGAVIRALTEETGTQINIEEDGTITIASNDSAKADEAKRRIAEITAEVEIGKVYEGAITKILDFGALV
NLLPGKDGLLHISQIAHERVEKVTDYLSEGQIVKVKVLETDEKGRVKLSMKALLDRPAQNQDQDRG

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=705, Percent_Identity=38.4397163120567, Blast_Score=439, Evalue=1e-123,
Organism=Escherichia coli, GI145693187, Length=690, Percent_Identity=65.9420289855072, Blast_Score=935, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=709, Percent_Identity=32.5811001410437, Blast_Score=314, Evalue=1e-85,
Organism=Caenorhabditis elegans, GI17535281, Length=74, Percent_Identity=48.6486486486487, Blast_Score=66, Evalue=8e-11,
Organism=Drosophila melanogaster, GI281362905, Length=713, Percent_Identity=37.4474053295933, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651641, Length=713, Percent_Identity=37.4474053295933, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651643, Length=713, Percent_Identity=37.4474053295933, Blast_Score=435, Evalue=1e-122,
Organism=Drosophila melanogaster, GI161079377, Length=651, Percent_Identity=36.8663594470046, Blast_Score=393, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_POLSJ (Q127W8)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_550072.1
- ProteinModelPortal:   Q127W8
- STRING:   Q127W8
- GeneID:   4014069
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_3260
- NMPDR:   fig|296591.1.peg.1200
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q127W8
- ProtClustDB:   PRK11824
- BioCyc:   PSP296591:BPRO_3260-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 76723; Mature: 76592

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1; PS01228 COF_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MSIFNKVTKSFQWGQHKVTMETGEVARQASGAVVVDMDGTVVLATVVAKTDAKPGQDFFP
CCHHHHHHHHHCCCCEEEEEEHHHHHHHCCCEEEEECCCCEEEEEEHHHCCCCCCCCCCC
LTVDYLEKTYAAGRIPGSFFKREGRPSEFETLTSRLIDRPIRPLFPEGFFNEVQVVIHVL
CHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
SLNPEVEGDIPALIASSAALSISGIPFNGPIGAARVAYVDGQYVLNPGKTQLKDSKMDLV
HCCCCCCCCCHHHHHCCCEEEEECCCCCCCCCCCEEEEECCEEEECCCCCCCCCCCEEEE
VAGTEAAVLMVESEAQQLSEEIMLGAVVFGHEQANIAINAIHELVRDAGKPVWDWQAPAK
EECCCEEEEEEEHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
DEPLIAKVNELAGAKLQAAYQIRSKQARTQACRVAYADVMAALKADGVAFDGVTVEGMLF
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEEEE
DIEAKIVRSQILAGEPRIDGRDTRTVRAIEIRNSVLPRTHGSALFTRGETQALVVTTLGT
EHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCEEEEEECCC
ERDAQRIDALSGDYEDRFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALIAVLPTK
CHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHEEEEEECCC
EEFPYTMRVVSEITESNGSSSMASVCGGCLSLMDAGVPMKAHVAGIAMGLIKEENRFAVL
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCEEEE
TDILGDEDHLGDMDFKVAGTTFGITALQMDIKIQGITKEIMQVALAQAKEARMHILGKMQ
EECCCCCCCCCCCCEEEECCEEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
EAMGEAKAEVSDFAPRLYVMKINPEKIRDVIGKGGAVIRALTEETGTQINIEEDGTITIA
HHHHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCEEEEEHHCCCCEEEEECCCEEEEE
SNDSAKADEAKRRIAEITAEVEIGKVYEGAITKILDFGALVNLLPGKDGLLHISQIAHER
CCCCCCHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
VEKVTDYLSEGQIVKVKVLETDEKGRVKLSMKALLDRPAQNQDQDRG
HHHHHHHHCCCCEEEEEEEECCCCCCEEEEHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SIFNKVTKSFQWGQHKVTMETGEVARQASGAVVVDMDGTVVLATVVAKTDAKPGQDFFP
CHHHHHHHHHCCCCEEEEEEHHHHHHHCCCEEEEECCCCEEEEEEHHHCCCCCCCCCCC
LTVDYLEKTYAAGRIPGSFFKREGRPSEFETLTSRLIDRPIRPLFPEGFFNEVQVVIHVL
CHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
SLNPEVEGDIPALIASSAALSISGIPFNGPIGAARVAYVDGQYVLNPGKTQLKDSKMDLV
HCCCCCCCCCHHHHHCCCEEEEECCCCCCCCCCCEEEEECCEEEECCCCCCCCCCCEEEE
VAGTEAAVLMVESEAQQLSEEIMLGAVVFGHEQANIAINAIHELVRDAGKPVWDWQAPAK
EECCCEEEEEEEHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
DEPLIAKVNELAGAKLQAAYQIRSKQARTQACRVAYADVMAALKADGVAFDGVTVEGMLF
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEEEEEEE
DIEAKIVRSQILAGEPRIDGRDTRTVRAIEIRNSVLPRTHGSALFTRGETQALVVTTLGT
EHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEEEECCCCCEEEEEECCC
ERDAQRIDALSGDYEDRFMLHYNMPPFATGETGRVGSPKRREIGHGRLAKRALIAVLPTK
CHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHEEEEEECCC
EEFPYTMRVVSEITESNGSSSMASVCGGCLSLMDAGVPMKAHVAGIAMGLIKEENRFAVL
CCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEECCCCEEEE
TDILGDEDHLGDMDFKVAGTTFGITALQMDIKIQGITKEIMQVALAQAKEARMHILGKMQ
EECCCCCCCCCCCCEEEECCEEEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
EAMGEAKAEVSDFAPRLYVMKINPEKIRDVIGKGGAVIRALTEETGTQINIEEDGTITIA
HHHHHHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCEEEEEHHCCCCEEEEECCCEEEEE
SNDSAKADEAKRRIAEITAEVEIGKVYEGAITKILDFGALVNLLPGKDGLLHISQIAHER
CCCCCCHHHHHHHHHHHHHHEEECHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
VEKVTDYLSEGQIVKVKVLETDEKGRVKLSMKALLDRPAQNQDQDRG
HHHHHHHHCCCCEEEEEEEECCCCCCEEEEHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA