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The map label for this gene is grpE

Identifier: 91788985

GI number: 91788985

Start: 3298511

End: 3299071

Strand: Direct

Name: grpE

Synonym: Bpro_3125

Alternate gene names: 91788985

Gene position: 3298511-3299071 (Clockwise)

Preceding gene: 91788984

Following gene: 91788986

Centisome position: 63.43

GC content: 62.03

Gene sequence:

>561_bases
ATGTCTGAAAATACACAACCCGAGCAAAATCAACCACTTACCGGAGCGCCTAGCCCCGAAGAGCTGGAAGCCGCGCAAGC
CGCCAACGAGTTTGACGCGCTGACCCAGGCCCAGGCTGAACTGGTCACCCTGCAGGCCAAAAACACCGAGCTGGCCGACA
GCTACCTGCGCGCCAAGGCCGAGACGGAAAACGCCCGCCGCCGCGCTGACGACGAAATTGCCAAGGCACGCAAATTTGCG
CTGGAAAGCTTTGCGGAAAGCCTGCTTCCCGTTGTCGACAGCCTCGAGGCCGGCCTGGCACACAAGGACGCAACGCCCGA
ACAGATCCGGGAGGGTGCGGACGCCACGCTCAAGCAACTCAAAACCACCCTCGAGCGCAACAAGATCGTGGAAATCAACC
CCGCCAGCGGAAGCCGGTTTGACCCGCACCAGCACCAGGCGATCAGCATGGTGCCCGCCGAGCAGGAAGCCAACACCGTA
GTCAGCGTGCTGCAAAAAGGCTACCTGATTTCCGACCGCGTGCTGCGCCCCGCCCTGGTCACCGTAACCGCGCCGAAATA
A

Upstream 100 bases:

>100_bases
CACGCCGAAAATTTCGCCCTCAAAACCGAATCCCTTGTCTTGAAAAGGGCTTTGTAACCCCCAGTTAACCCCCTTGTTAG
CTTAAACCTTGTCTTCAAGC

Downstream 100 bases:

>100_bases
ACGCATAAATCAGGCTGAGGCACTTGAACTCGTACAAGTTATCCACAAGTCATCAACATGCTCATTCACAGCGACTGAGC
CATCAGCCACACCACCATCC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MSENTQPEQNQPLTGAPSPEELEAAQAANEFDALTQAQAELVTLQAKNTELADSYLRAKAETENARRRADDEIAKARKFA
LESFAESLLPVVDSLEAGLAHKDATPEQIREGADATLKQLKTTLERNKIVEINPASGSRFDPHQHQAISMVPAEQEANTV
VSVLQKGYLISDRVLRPALVTVTAPK

Sequences:

>Translated_186_residues
MSENTQPEQNQPLTGAPSPEELEAAQAANEFDALTQAQAELVTLQAKNTELADSYLRAKAETENARRRADDEIAKARKFA
LESFAESLLPVVDSLEAGLAHKDATPEQIREGADATLKQLKTTLERNKIVEINPASGSRFDPHQHQAISMVPAEQEANTV
VSVLQKGYLISDRVLRPALVTVTAPK
>Mature_185_residues
SENTQPEQNQPLTGAPSPEELEAAQAANEFDALTQAQAELVTLQAKNTELADSYLRAKAETENARRRADDEIAKARKFAL
ESFAESLLPVVDSLEAGLAHKDATPEQIREGADATLKQLKTTLERNKIVEINPASGSRFDPHQHQAISMVPAEQEANTVV
SVLQKGYLISDRVLRPALVTVTAPK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=153, Percent_Identity=33.9869281045752, Blast_Score=84, Evalue=7e-17,
Organism=Homo sapiens, GI40255109, Length=152, Percent_Identity=30.9210526315789, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1788967, Length=138, Percent_Identity=43.4782608695652, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17552458, Length=167, Percent_Identity=32.3353293413174, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324806, Length=164, Percent_Identity=32.9268292682927, Blast_Score=76, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24653432, Length=173, Percent_Identity=33.5260115606936, Blast_Score=84, Evalue=5e-17,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_POLSJ (Q128K3)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_549937.1
- ProteinModelPortal:   Q128K3
- SMR:   Q128K3
- STRING:   Q128K3
- GeneID:   4014205
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_3125
- NMPDR:   fig|296591.1.peg.2878
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- PhylomeDB:   Q128K3
- ProtClustDB:   PRK14139
- BioCyc:   PSP296591:BPRO_3125-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 20254; Mature: 20123

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENTQPEQNQPLTGAPSPEELEAAQAANEFDALTQAQAELVTLQAKNTELADSYLRAKA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHH
ETENARRRADDEIAKARKFALESFAESLLPVVDSLEAGLAHKDATPEQIREGADATLKQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHH
KTTLERNKIVEINPASGSRFDPHQHQAISMVPAEQEANTVVSVLQKGYLISDRVLRPALV
HHHHHHCCEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHCCEEE
TVTAPK
EEECCC
>Mature Secondary Structure 
SENTQPEQNQPLTGAPSPEELEAAQAANEFDALTQAQAELVTLQAKNTELADSYLRAKA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHH
ETENARRRADDEIAKARKFALESFAESLLPVVDSLEAGLAHKDATPEQIREGADATLKQL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCHHHHHHHH
KTTLERNKIVEINPASGSRFDPHQHQAISMVPAEQEANTVVSVLQKGYLISDRVLRPALV
HHHHHHCCEEEEECCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHCCEEE
TVTAPK
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA