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The map label for this gene is ahpF [H]

Identifier: 91788905

GI number: 91788905

Start: 3210626

End: 3212197

Strand: Direct

Name: ahpF [H]

Synonym: Bpro_3045

Alternate gene names: 91788905

Gene position: 3210626-3212197 (Clockwise)

Preceding gene: 91788904

Following gene: 91788906

Centisome position: 61.74

GC content: 62.79

Gene sequence:

>1572_bases
ATGCTCGACGCCAGCACCAAAGCCCAACTGAAGAGTTACCTCGAGCGCGCCACGCAGCCGATCGAAATCATCGCGTCGCT
CGACGACGGCAAGGCCTCGGGCGAGCTGCTGTCGCTGCTCAAGGATGTGGCTGAATCGTCGCCGCTGGTCACGCTGACCG
AAAGCCGCGACGACCCGCACCGCAAGCCCTCGTTCTCGGTCAACCGCCCGGGCGAAAACCACGGCCCGCGTTTTGCCGGC
CTGCCCATGGGCCATGAATTCACCTCGCTGATCCTGGCGCTGCTGCAGGTCGGCGGCTACCCGCCGAGAGTCGAGCAGGC
CGTGTTGGAGCAGATTCGTGCGCTCGACGGGGATTTTGAATTTGAGATTTATGTTTCACTTACCTGCCACAACTGCCCCG
ACGTGGTCCAGGCGCTGAACCTGATGGCGGTGCAGAACCCGCGTATCCGCACCACCATGATCGAAGGCGGCACCTTCCAG
GACGAGGTCAAGGAGCGCCAGGTCATGGCCGTGCCCACCGTCTTTTTGAACGGCACCGAGTTCGGCCAGGGCCGCATGAG
CCTGGAAGAAATCCTGGCAAAAATCGACACCAGCGGTGTGGAACACGAAGCGGCCAAAATCGCCGCCAAGGACCCGTTCG
ACGTGCTGATCGTCGGCGGCGGCCCGGCCGGTGCCGCAGCGGCGGTGTACGCCGCGCGCAAAGGCATACGCACCGGCGTG
GCGTCGGAGCGTTTCGGCGGCCAGGTGCTGGACACCATGGGCATCGAGAATTTCATCTCGATCAAGGAAACCGAGGGGCC
GAAATTCGCGCACGCGCTGGAAGAACACGTGCGCAACTATGACGTCGACATCATGAACCTGCAGCGCGCCAAGGCGCTGG
TTCCGAATACCGGACCAGGCAAGGACCTGATCGAGATCCAGCTTGAAAGCGGTGCCTCGCTCAAAAGCAGGTCGATCGTC
ATATCGACCGGCGCGCGCTGGCGCAACATCAACGTGCCGGGCGAGCGCGAGTACAAAAACAAGGGCGTGGCCTACTGCCC
GCACTGCGACGGCCCGTTGTTCAAGGGCAAGCGCGTGGCGGTGATAGGCGGCGGCAACTCCGGTGTCGAAGCGGCGATCG
ATCTGGCCGGCATCGTCGGGCATGTGACGCTGATCGAGTTCGACACTGCGCTGCGTGCCGATGCCGTGCTGCAGCGCAAG
CTCACCAGCCTGAAGAACGTGACCGTGTTCACCCATGCGCAGACCACCGAGATCACCGGTGACCAGAAGGTCAATGGCCT
CATCTACAAGGAGCGTGCAAGCGGCGAGTTGAAGAAGGTCGAACTGGAAGGCGTGTTCATCCAGATCGGCCTGGTACCCA
ACACCGACTGGCTCAAGGGCGTGGTTGAGTTGTCCCGGCATGGCGAGATCGTGGTGGATGCCAAGGGCCAGACCTCTGTG
CCTGGCGTATTTGCAGCCGGCGATGTGACGACCGTGCCTTTCAAGCAGATCATCATCGCGGCAGGCGAAGGCGCAAAGGC
CGCGCTGAGCGCTTTCGATCACCTGATCCGCACTTCAGCACCGGCAGCCTGA

Upstream 100 bases:

>100_bases
CTGGTCGGCAAGATCTAAGCCCAGCCTGTTTGCGCCAAGCCCGGGCGCGCCAGCTCCCGGGCTTTTTTATTCCCCCATGC
ATTCGAAAAGTGAGTTCCCC

Downstream 100 bases:

>100_bases
AGCCATGCGCCAGTTGGCCGGCAGCATGTTTCTGCAAAGAAGAACGCCTATCAATCCCATTCGCCCTCAAAACGCACCTG
TAAGGTCATGGCATTTTGCT

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 523; Mature: 523

Protein sequence:

>523_residues
MLDASTKAQLKSYLERATQPIEIIASLDDGKASGELLSLLKDVAESSPLVTLTESRDDPHRKPSFSVNRPGENHGPRFAG
LPMGHEFTSLILALLQVGGYPPRVEQAVLEQIRALDGDFEFEIYVSLTCHNCPDVVQALNLMAVQNPRIRTTMIEGGTFQ
DEVKERQVMAVPTVFLNGTEFGQGRMSLEEILAKIDTSGVEHEAAKIAAKDPFDVLIVGGGPAGAAAAVYAARKGIRTGV
ASERFGGQVLDTMGIENFISIKETEGPKFAHALEEHVRNYDVDIMNLQRAKALVPNTGPGKDLIEIQLESGASLKSRSIV
ISTGARWRNINVPGEREYKNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVGHVTLIEFDTALRADAVLQRK
LTSLKNVTVFTHAQTTEITGDQKVNGLIYKERASGELKKVELEGVFIQIGLVPNTDWLKGVVELSRHGEIVVDAKGQTSV
PGVFAAGDVTTVPFKQIIIAAGEGAKAALSAFDHLIRTSAPAA

Sequences:

>Translated_523_residues
MLDASTKAQLKSYLERATQPIEIIASLDDGKASGELLSLLKDVAESSPLVTLTESRDDPHRKPSFSVNRPGENHGPRFAG
LPMGHEFTSLILALLQVGGYPPRVEQAVLEQIRALDGDFEFEIYVSLTCHNCPDVVQALNLMAVQNPRIRTTMIEGGTFQ
DEVKERQVMAVPTVFLNGTEFGQGRMSLEEILAKIDTSGVEHEAAKIAAKDPFDVLIVGGGPAGAAAAVYAARKGIRTGV
ASERFGGQVLDTMGIENFISIKETEGPKFAHALEEHVRNYDVDIMNLQRAKALVPNTGPGKDLIEIQLESGASLKSRSIV
ISTGARWRNINVPGEREYKNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVGHVTLIEFDTALRADAVLQRK
LTSLKNVTVFTHAQTTEITGDQKVNGLIYKERASGELKKVELEGVFIQIGLVPNTDWLKGVVELSRHGEIVVDAKGQTSV
PGVFAAGDVTTVPFKQIIIAAGEGAKAALSAFDHLIRTSAPAA
>Mature_523_residues
MLDASTKAQLKSYLERATQPIEIIASLDDGKASGELLSLLKDVAESSPLVTLTESRDDPHRKPSFSVNRPGENHGPRFAG
LPMGHEFTSLILALLQVGGYPPRVEQAVLEQIRALDGDFEFEIYVSLTCHNCPDVVQALNLMAVQNPRIRTTMIEGGTFQ
DEVKERQVMAVPTVFLNGTEFGQGRMSLEEILAKIDTSGVEHEAAKIAAKDPFDVLIVGGGPAGAAAAVYAARKGIRTGV
ASERFGGQVLDTMGIENFISIKETEGPKFAHALEEHVRNYDVDIMNLQRAKALVPNTGPGKDLIEIQLESGASLKSRSIV
ISTGARWRNINVPGEREYKNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVGHVTLIEFDTALRADAVLQRK
LTSLKNVTVFTHAQTTEITGDQKVNGLIYKERASGELKKVELEGVFIQIGLVPNTDWLKGVVELSRHGEIVVDAKGQTSV
PGVFAAGDVTTVPFKQIIIAAGEGAKAALSAFDHLIRTSAPAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=519, Percent_Identity=63.9691714836224, Blast_Score=674, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=316, Percent_Identity=35.126582278481, Blast_Score=156, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6321898, Length=315, Percent_Identity=32.6984126984127, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320560, Length=315, Percent_Identity=32.3809523809524, Blast_Score=139, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56057; Mature: 56057

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDASTKAQLKSYLERATQPIEIIASLDDGKASGELLSLLKDVAESSPLVTLTESRDDPH
CCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCC
RKPSFSVNRPGENHGPRFAGLPMGHEFTSLILALLQVGGYPPRVEQAVLEQIRALDGDFE
CCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEE
FEIYVSLTCHNCPDVVQALNLMAVQNPRIRTTMIEGGTFQDEVKERQVMAVPTVFLNGTE
EEEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHCEEEEECEEEECCCC
FGQGRMSLEEILAKIDTSGVEHEAAKIAAKDPFDVLIVGGGPAGAAAAVYAARKGIRTGV
CCCCCHHHHHHHHHHCCCCCCHHHHHEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCC
ASERFGGQVLDTMGIENFISIKETEGPKFAHALEEHVRNYDVDIMNLQRAKALVPNTGPG
CCHHCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCC
KDLIEIQLESGASLKSRSIVISTGARWRNINVPGEREYKNKGVAYCPHCDGPLFKGKRVA
CCEEEEEECCCCCCCCCEEEEECCCCEEECCCCCCHHHCCCCCEECCCCCCCCCCCCEEE
VIGGGNSGVEAAIDLAGIVGHVTLIEFDTALRADAVLQRKLTSLKNVTVFTHAQTTEITG
EEECCCCCHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHHHHHCCEEEEEECCEEECCC
DQKVNGLIYKERASGELKKVELEGVFIQIGLVPNTDWLKGVVELSRHGEIVVDAKGQTSV
CCCCCCEEEEECCCCCEEEEEEEEEEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCC
PGVFAAGDVTTVPFKQIIIAAGEGAKAALSAFDHLIRTSAPAA
CCEEECCCEEECCCEEEEEECCCCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLDASTKAQLKSYLERATQPIEIIASLDDGKASGELLSLLKDVAESSPLVTLTESRDDPH
CCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCC
RKPSFSVNRPGENHGPRFAGLPMGHEFTSLILALLQVGGYPPRVEQAVLEQIRALDGDFE
CCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEE
FEIYVSLTCHNCPDVVQALNLMAVQNPRIRTTMIEGGTFQDEVKERQVMAVPTVFLNGTE
EEEEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHCEEEEECEEEECCCC
FGQGRMSLEEILAKIDTSGVEHEAAKIAAKDPFDVLIVGGGPAGAAAAVYAARKGIRTGV
CCCCCHHHHHHHHHHCCCCCCHHHHHEECCCCCEEEEECCCCCHHHHHHHHHHHHHHCCC
ASERFGGQVLDTMGIENFISIKETEGPKFAHALEEHVRNYDVDIMNLQRAKALVPNTGPG
CCHHCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEHHHHHHCCCCCCCC
KDLIEIQLESGASLKSRSIVISTGARWRNINVPGEREYKNKGVAYCPHCDGPLFKGKRVA
CCEEEEEECCCCCCCCCEEEEECCCCEEECCCCCCHHHCCCCCEECCCCCCCCCCCCEEE
VIGGGNSGVEAAIDLAGIVGHVTLIEFDTALRADAVLQRKLTSLKNVTVFTHAQTTEITG
EEECCCCCHHHHHHHHHHHHEEEEEEECHHHHHHHHHHHHHHHHCCEEEEEECCEEECCC
DQKVNGLIYKERASGELKKVELEGVFIQIGLVPNTDWLKGVVELSRHGEIVVDAKGQTSV
CCCCCCEEEEECCCCCEEEEEEEEEEEEEEECCCCHHHHHHHHHHCCCCEEEECCCCCCC
PGVFAAGDVTTVPFKQIIIAAGEGAKAALSAFDHLIRTSAPAA
CCEEECCCEEECCCEEEEEECCCCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]