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The map label for this gene is rhtB [H]

Identifier: 91788845

GI number: 91788845

Start: 3140853

End: 3141479

Strand: Direct

Name: rhtB [H]

Synonym: Bpro_2985

Alternate gene names: 91788845

Gene position: 3140853-3141479 (Clockwise)

Preceding gene: 91788844

Following gene: 91788846

Centisome position: 60.4

GC content: 62.52

Gene sequence:

>627_bases
ATGACGCTCACCACCTACCTCCTCTACATAGCGGCCGTCGCGCTGCTCATCCTGACACCGGGCCCGACCATGCTGATGTG
CATGACGAATGCCCTGAACCACGGGCCACGCAAGGCCATGACCTCGGTTGCCGGAAGCGTCAGCGCTGTGCTGTGTGTCA
TGCTGCTGTCGGCCATGGGCCTGGGCGCGCTGCTGGCGACATCCGAGACGGCCTTCACCGTGGCCAAGGTCATCGGTGCT
GCCTACCTGATCTGGCTGGGCATCAAGACCTTTCGCAGCGAGGCCGCCGTGCTGGACGCCAGCGCAGAGCGTTCGCCACA
GCAGCGCCGCTCGTTTTTCCTGCGCGGTTTTCTGGTCGGCGCCAGCAATCCCAAGGCATTGCTGTTTTTTGCGGCCTTTT
TTCCGCAATTCCTGAACCCCGCCGCACCCTTTGTGCCGCAATTCGCCATCCTCGCCCTCACCTTCATGGCCTTTGAATTC
ACGGTGCTCACCTGCTGCGCGCTGGGCGTTTCACGCATCGCGCCTTTGCTGCGCTCCAGCCGCGCCGTACGATGGTTCAA
CCGCGGTTCGGGCAGTCTTTTCACGCTCATGGGCAGTCTGTTATTGCTGACACGCCGGCATACCTGA

Upstream 100 bases:

>100_bases
ACGCTCGTTCACTGCGCGCAGCTAGCCGTTCTGCCTCATTTTTTGAACAAACTGGTACGGCGCGTGCTTGCCGTCTTGTC
GCGATACTATCTTCACCACC

Downstream 100 bases:

>100_bases
GACCGATTTATGGACATAGCCAACTTCCTCATCCAGCTGCTCAACAGCGTGCAGTACGGCCTCTTGCTGTTCATGCTGGC
GGCCGGCCTGACCCTGATCT

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 207

Protein sequence:

>208_residues
MTLTTYLLYIAAVALLILTPGPTMLMCMTNALNHGPRKAMTSVAGSVSAVLCVMLLSAMGLGALLATSETAFTVAKVIGA
AYLIWLGIKTFRSEAAVLDASAERSPQQRRSFFLRGFLVGASNPKALLFFAAFFPQFLNPAAPFVPQFAILALTFMAFEF
TVLTCCALGVSRIAPLLRSSRAVRWFNRGSGSLFTLMGSLLLLTRRHT

Sequences:

>Translated_208_residues
MTLTTYLLYIAAVALLILTPGPTMLMCMTNALNHGPRKAMTSVAGSVSAVLCVMLLSAMGLGALLATSETAFTVAKVIGA
AYLIWLGIKTFRSEAAVLDASAERSPQQRRSFFLRGFLVGASNPKALLFFAAFFPQFLNPAAPFVPQFAILALTFMAFEF
TVLTCCALGVSRIAPLLRSSRAVRWFNRGSGSLFTLMGSLLLLTRRHT
>Mature_207_residues
TLTTYLLYIAAVALLILTPGPTMLMCMTNALNHGPRKAMTSVAGSVSAVLCVMLLSAMGLGALLATSETAFTVAKVIGAA
YLIWLGIKTFRSEAAVLDASAERSPQQRRSFFLRGFLVGASNPKALLFFAAFFPQFLNPAAPFVPQFAILALTFMAFEFT
VLTCCALGVSRIAPLLRSSRAVRWFNRGSGSLFTLMGSLLLLTRRHT

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=206, Percent_Identity=38.3495145631068, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1788099, Length=178, Percent_Identity=26.4044943820225, Blast_Score=81, Evalue=4e-17,
Organism=Escherichia coli, GI48994978, Length=212, Percent_Identity=26.4150943396226, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22393; Mature: 22262

Theoretical pI: Translated: 11.40; Mature: 11.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTTYLLYIAAVALLILTPGPTMLMCMTNALNHGPRKAMTSVAGSVSAVLCVMLLSAMG
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
LGALLATSETAFTVAKVIGAAYLIWLGIKTFRSEAAVLDASAERSPQQRRSFFLRGFLVG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHEEC
ASNPKALLFFAAFFPQFLNPAAPFVPQFAILALTFMAFEFTVLTCCALGVSRIAPLLRSS
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RAVRWFNRGSGSLFTLMGSLLLLTRRHT
HHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLTTYLLYIAAVALLILTPGPTMLMCMTNALNHGPRKAMTSVAGSVSAVLCVMLLSAMG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHC
LGALLATSETAFTVAKVIGAAYLIWLGIKTFRSEAAVLDASAERSPQQRRSFFLRGFLVG
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHEEC
ASNPKALLFFAAFFPQFLNPAAPFVPQFAILALTFMAFEFTVLTCCALGVSRIAPLLRSS
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RAVRWFNRGSGSLFTLMGSLLLLTRRHT
HHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]