Click here to switch to the map view.

The map label for this gene is oprM [H]

Identifier: 91788690

GI number: 91788690

Start: 2980777

End: 2982213

Strand: Direct

Name: oprM [H]

Synonym: Bpro_2829

Alternate gene names: 91788690

Gene position: 2980777-2982213 (Clockwise)

Preceding gene: 91788689

Following gene: 91788691

Centisome position: 57.32

GC content: 69.24

Gene sequence:

>1437_bases
ATGTACAAGGAATTGAAATTTCGCCGCTACCACTACGTGATGGCCGCGGCACTGGCCCTGCTGGCCACAGGCTGCGCGGT
CGGCCCGGATTACAAGCGCCCGGCCACCGCCGATGTCAGCGCCTTCAAGGAAGCCGAAGGCTGGGTGCCCGCCGCGCCGG
CCGATGCGCTGGAGCGCGGCCCGTGGTGGACGCTGTTTGGCGACCCGGTGCTGGACCAGCTGCAGTCGCGGGTCGAGGTG
TCCAACCAGAACGTGGCGCTGGCGGTGGCCGCCTATGCGCAGGCGCGTGCCCTGGTACGCGAGCAGCGGGCATCGCTCTT
TCCTTCGGTCACGCTCGATGGTGGAGCCAGCCGTTCGAAGAGCAGCGGCAGTGGCAGCAGCAGTTCTGGCGCTGGCGCTG
GCGGGCGCATTGGCAACAACTACCAGCTCAGCATAGGCGGCAGCTGGGAGCCCGACGTCTGGGGCCGGCTGGGCCGGGCC
GTCGACGGCGCCAGTGCCGGCGCCCAGGCGAGTGCGGCAGACCTGGCCTCGGCCAAGCTGTCGGCCCAGGGCGAACTCGC
CGTCAATTACCTTTCGCTGCGCCAGACCGATGCGCAAAAGGCCTTGCTGGAAAGCACGATTGCCGGCTACGAGCGTGCAC
TGGAGATCACGCAAAACCGCTACGCGGCCAGCATTGCCGCCAAGACCGATGTGCTGCAGGCGCAAACCCAACTGGCCAAC
GCCAGGGCTGAAGCCGCAGGGCTGGTGCGCCAGCGCGCCCAGCTCGAGCATGCCATCGCGGTGCTGGTGGGCGAGGCGCC
CGGTAATTTCACGCTCGCACCCGCCGCCTGGACACCCGTGGTGCCAGAGATTCCGGTCGGCGTGCCTTCCACGCTGCTGC
AGCGCCGGCCCGATATCGCGGCAGCCGAGCGCCGCGTGGCCCAGGCCAACGAGCAGATCGGCATCGCCAAAAGCGCCTAC
TTCCCCAGCCTGTCCTTGAGCGCATCGGCCGGCTCTGGCGCCAGCCGCCTCGCGGACCTGTTCAAGGCCTCCAGCAGTGT
CTGGTCGCTGGGCATTTCGGCCGCGCAGGTGCTGTTCAATGCTGGCGCGACGGGGGCCCGCGTCGAAGGTGCGCAGGCCT
CGCACGAGCAGGCAGTGGCGCGCTACCGGCAGGCCGTGCTGACAGCATTCCAGGATGTCGAGGACCAGCTTTCCGCCACC
CGCGTGCTGCTGGTCCAGCAGGACTTGCGGCGCCAGGCCTCTGACGCCGCCGACCAGGTCGAGCAACAGGTGTTCAACCG
CTACCGCGGCGGTCAGGTCGGCTACACCGAGGTCATCACCGCGCAGGCCACCGCGCTGAGCGCGCGCCGCGCGCTGGTGC
AGGCCATGGCCGACCGCCAAACCACCGCCGTGGCCTTGATCCAGTCGCTGGGCGGCGGTTGGCAGGATGGACGGTAA

Upstream 100 bases:

>100_bases
ATGATGACCGCCATAAACAATACTTGGCTTTGCCTCTTGCTCCTTCCCCCGCTGGTGGAAGGAGCAAGCGTGACGGTATG
ACACCCAAAGGTGGAAAAGT

Downstream 100 bases:

>100_bases
AGCGGCCACGCGGCGGCAGTCACTATAATTTTGATAGCAAATTACACCCAAATCCATTGGGCTGGGCACTGACTTCCCAT
CAAAACCCGGCCAAACGTGT

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MYKELKFRRYHYVMAAALALLATGCAVGPDYKRPATADVSAFKEAEGWVPAAPADALERGPWWTLFGDPVLDQLQSRVEV
SNQNVALAVAAYAQARALVREQRASLFPSVTLDGGASRSKSSGSGSSSSGAGAGGRIGNNYQLSIGGSWEPDVWGRLGRA
VDGASAGAQASAADLASAKLSAQGELAVNYLSLRQTDAQKALLESTIAGYERALEITQNRYAASIAAKTDVLQAQTQLAN
ARAEAAGLVRQRAQLEHAIAVLVGEAPGNFTLAPAAWTPVVPEIPVGVPSTLLQRRPDIAAAERRVAQANEQIGIAKSAY
FPSLSLSASAGSGASRLADLFKASSSVWSLGISAAQVLFNAGATGARVEGAQASHEQAVARYRQAVLTAFQDVEDQLSAT
RVLLVQQDLRRQASDAADQVEQQVFNRYRGGQVGYTEVITAQATALSARRALVQAMADRQTTAVALIQSLGGGWQDGR

Sequences:

>Translated_478_residues
MYKELKFRRYHYVMAAALALLATGCAVGPDYKRPATADVSAFKEAEGWVPAAPADALERGPWWTLFGDPVLDQLQSRVEV
SNQNVALAVAAYAQARALVREQRASLFPSVTLDGGASRSKSSGSGSSSSGAGAGGRIGNNYQLSIGGSWEPDVWGRLGRA
VDGASAGAQASAADLASAKLSAQGELAVNYLSLRQTDAQKALLESTIAGYERALEITQNRYAASIAAKTDVLQAQTQLAN
ARAEAAGLVRQRAQLEHAIAVLVGEAPGNFTLAPAAWTPVVPEIPVGVPSTLLQRRPDIAAAERRVAQANEQIGIAKSAY
FPSLSLSASAGSGASRLADLFKASSSVWSLGISAAQVLFNAGATGARVEGAQASHEQAVARYRQAVLTAFQDVEDQLSAT
RVLLVQQDLRRQASDAADQVEQQVFNRYRGGQVGYTEVITAQATALSARRALVQAMADRQTTAVALIQSLGGGWQDGR
>Mature_478_residues
MYKELKFRRYHYVMAAALALLATGCAVGPDYKRPATADVSAFKEAEGWVPAAPADALERGPWWTLFGDPVLDQLQSRVEV
SNQNVALAVAAYAQARALVREQRASLFPSVTLDGGASRSKSSGSGSSSSGAGAGGRIGNNYQLSIGGSWEPDVWGRLGRA
VDGASAGAQASAADLASAKLSAQGELAVNYLSLRQTDAQKALLESTIAGYERALEITQNRYAASIAAKTDVLQAQTQLAN
ARAEAAGLVRQRAQLEHAIAVLVGEAPGNFTLAPAAWTPVVPEIPVGVPSTLLQRRPDIAAAERRVAQANEQIGIAKSAY
FPSLSLSASAGSGASRLADLFKASSSVWSLGISAAQVLFNAGATGARVEGAQASHEQAVARYRQAVLTAFQDVEDQLSAT
RVLLVQQDLRRQASDAADQVEQQVFNRYRGGQVGYTEVITAQATALSARRALVQAMADRQTTAVALIQSLGGGWQDGR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=465, Percent_Identity=27.9569892473118, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI2367348, Length=443, Percent_Identity=26.1851015801354, Blast_Score=106, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 50166; Mature: 50166

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKELKFRRYHYVMAAALALLATGCAVGPDYKRPATADVSAFKEAEGWVPAAPADALERG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHCC
PWWTLFGDPVLDQLQSRVEVSNQNVALAVAAYAQARALVREQRASLFPSVTLDGGASRSK
CCEEECCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCC
SSGSGSSSSGAGAGGRIGNNYQLSIGGSWEPDVWGRLGRAVDGASAGAQASAADLASAKL
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SAQGELAVNYLSLRQTDAQKALLESTIAGYERALEITQNRYAASIAAKTDVLQAQTQLAN
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARAEAAGLVRQRAQLEHAIAVLVGEAPGNFTLAPAAWTPVVPEIPVGVPSTLLQRRPDIA
HHHHHHHHHHHHHHHHHHHHEEEECCCCCEEECCCCCCCCCCCCCCCCCHHHHHCCCCHH
AAERRVAQANEQIGIAKSAYFPSLSLSASAGSGASRLADLFKASSSVWSLGISAAQVLFN
HHHHHHHHHHHHCCCHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGATGARVEGAQASHEQAVARYRQAVLTAFQDVEDQLSATRVLLVQQDLRRQASDAADQV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQQVFNRYRGGQVGYTEVITAQATALSARRALVQAMADRQTTAVALIQSLGGGWQDGR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MYKELKFRRYHYVMAAALALLATGCAVGPDYKRPATADVSAFKEAEGWVPAAPADALERG
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHCC
PWWTLFGDPVLDQLQSRVEVSNQNVALAVAAYAQARALVREQRASLFPSVTLDGGASRSK
CCEEECCCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCC
SSGSGSSSSGAGAGGRIGNNYQLSIGGSWEPDVWGRLGRAVDGASAGAQASAADLASAKL
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
SAQGELAVNYLSLRQTDAQKALLESTIAGYERALEITQNRYAASIAAKTDVLQAQTQLAN
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARAEAAGLVRQRAQLEHAIAVLVGEAPGNFTLAPAAWTPVVPEIPVGVPSTLLQRRPDIA
HHHHHHHHHHHHHHHHHHHHEEEECCCCCEEECCCCCCCCCCCCCCCCCHHHHHCCCCHH
AAERRVAQANEQIGIAKSAYFPSLSLSASAGSGASRLADLFKASSSVWSLGISAAQVLFN
HHHHHHHHHHHHCCCHHHHCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGATGARVEGAQASHEQAVARYRQAVLTAFQDVEDQLSATRVLLVQQDLRRQASDAADQV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQQVFNRYRGGQVGYTEVITAQATALSARRALVQAMADRQTTAVALIQSLGGGWQDGR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]