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The map label for this gene is cobB [H]

Identifier: 91788647

GI number: 91788647

Start: 2925644

End: 2927002

Strand: Reverse

Name: cobB [H]

Synonym: Bpro_2785

Alternate gene names: 91788647

Gene position: 2927002-2925644 (Counterclockwise)

Preceding gene: 91788648

Following gene: 91788646

Centisome position: 56.29

GC content: 68.58

Gene sequence:

>1359_bases
ATGTCCGCCTCCACCTGCCCCGCCATTCTTGTCGCCGCCCCGGCCTCCGGCCAGGGCAAGACAACGGTGGCCTGCGCCCT
GGCGCGGTTGCACACACGCCAGGGCAGGCGGGTGCGTGTCTTCAAGTGCGGGCCGGATTTTCTGGACCCGTACTGGCTGA
CCCTGGCCAGCGGCGCGCCGGTGCACCAGCTCGATTTGTGGATGACCGGCGAGGCCGACTGCGCACAGCGCCTGCATGCC
GCCGCGCAGGAGGCTGACCTCATCATTGTGGAAGGGGTCATGGGGCTGTTTGACGGTGAGCCCAGCGCCGCGGACCTGGC
GCAGCGCTTTGGCCTGCCGGTGCTGGCGGTGATTGACGCCAGCGCCATGGCCGGCACCTTTGGCGCGCTGACGTGGGGCC
TGCAGCATTACCGCGAAGGCCTGCGGTTCGCCGGCGTGCTGGCCAACCGCGTGGCCTCGCCGGGTCATGCCGCGATGCTG
GAAGGCGGCCTGCGCGATGCAGCGCAGTGGCAGGGCGCGCTGATGCGCAGCGAGGCTTTTACGCTGCCCGAGCGGCATCT
GGGCCTGGTCATGTCGGATGAACTGGGCGACGCCCTGCCGCGGCTGGACGCGGCCGCCGACGCGCTGGCGGGTACGCCGC
TGGGGCAGATGGGAGCGACAGAGCTGCAGGCCTGGTCCGTGGCCTTCGAAGCGCCTGAAACGCTGGGCGAAGCTTTCCCC
GCCGGTGCATTGCAGGGCCGTACCATCGCGGTAGCGCGTGATGCGGCCTTCTGTTTTCTCTACGCTGCCAACCTCGACTG
TTTGCGTGCGCTGGGGGCTCAGTTGGTGTTTTTCTCGCCCCTGGCAGACACGGCTTTGCCTGAATGCGATGCCCTTTGGC
TGCCCGGCGGCTACCCCGAACTGCACGGCGAAACCCTGGCGGCCAACCGCGCCATGAAAAGCAGTATCACCGCCCATGTG
CAGGCCGGCAGGCCGGTCTGGGCCGAATGCGGCGGCATGATGGCGCTGTTTGAGCAGCTCGTGACAGTCGATGGCGCCAG
CTACCCCATGTGGGGCGTGCTGCCCGGCAACGTCAGCATGCAAAAACGTCTCGCCGCCCTGGGCCCCCAGCAGCTCGATG
TGGCGGGCGGTACCTTGCGCGGCCATGCTTTTCATTACTCCACCTGCGCCACGCCGCTGCAAGCCGTCGCGCGAACCCGG
GCGGCCCCCGGCCGCACGCTGCGTGGTGAGGGCGAGGCGCTGTTTGTGGCCGGGCCGACCTCCTCGGTGAGGGCCAGTTA
TTTTCACGCCTGGTTCGCCTCCTGCCCGGCTGCGACCGCGCAGTTGTTTGGCGCGCCTGTTTCTGAAAGATCGCCATGA

Upstream 100 bases:

>100_bases
TGAAGCGGGGCGTGCAATCGTCGCTGCGGTGCGGATTAAGTTCTAGAGGTTCTGGAGTAAGTCATCCATGCCGGATCGTT
AAGCGCAATCCCAACAGACC

Downstream 100 bases:

>100_bases
CTGACAAGAAAATGGGCTGGCGTGCGCTGGCTGACCAGCAGGACACCTCGGCAGTCCGTCAAATGTATGGCCCGCAGCGC
ATCGTCTGCCTGACGGAAGA

Product: hydrogenobyrinic acid a,c-diamide synthase/cobyrinate a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSASTCPAILVAAPASGQGKTTVACALARLHTRQGRRVRVFKCGPDFLDPYWLTLASGAPVHQLDLWMTGEADCAQRLHA
AAQEADLIIVEGVMGLFDGEPSAADLAQRFGLPVLAVIDASAMAGTFGALTWGLQHYREGLRFAGVLANRVASPGHAAML
EGGLRDAAQWQGALMRSEAFTLPERHLGLVMSDELGDALPRLDAAADALAGTPLGQMGATELQAWSVAFEAPETLGEAFP
AGALQGRTIAVARDAAFCFLYAANLDCLRALGAQLVFFSPLADTALPECDALWLPGGYPELHGETLAANRAMKSSITAHV
QAGRPVWAECGGMMALFEQLVTVDGASYPMWGVLPGNVSMQKRLAALGPQQLDVAGGTLRGHAFHYSTCATPLQAVARTR
AAPGRTLRGEGEALFVAGPTSSVRASYFHAWFASCPAATAQLFGAPVSERSP

Sequences:

>Translated_452_residues
MSASTCPAILVAAPASGQGKTTVACALARLHTRQGRRVRVFKCGPDFLDPYWLTLASGAPVHQLDLWMTGEADCAQRLHA
AAQEADLIIVEGVMGLFDGEPSAADLAQRFGLPVLAVIDASAMAGTFGALTWGLQHYREGLRFAGVLANRVASPGHAAML
EGGLRDAAQWQGALMRSEAFTLPERHLGLVMSDELGDALPRLDAAADALAGTPLGQMGATELQAWSVAFEAPETLGEAFP
AGALQGRTIAVARDAAFCFLYAANLDCLRALGAQLVFFSPLADTALPECDALWLPGGYPELHGETLAANRAMKSSITAHV
QAGRPVWAECGGMMALFEQLVTVDGASYPMWGVLPGNVSMQKRLAALGPQQLDVAGGTLRGHAFHYSTCATPLQAVARTR
AAPGRTLRGEGEALFVAGPTSSVRASYFHAWFASCPAATAQLFGAPVSERSP
>Mature_451_residues
SASTCPAILVAAPASGQGKTTVACALARLHTRQGRRVRVFKCGPDFLDPYWLTLASGAPVHQLDLWMTGEADCAQRLHAA
AQEADLIIVEGVMGLFDGEPSAADLAQRFGLPVLAVIDASAMAGTFGALTWGLQHYREGLRFAGVLANRVASPGHAAMLE
GGLRDAAQWQGALMRSEAFTLPERHLGLVMSDELGDALPRLDAAADALAGTPLGQMGATELQAWSVAFEAPETLGEAFPA
GALQGRTIAVARDAAFCFLYAANLDCLRALGAQLVFFSPLADTALPECDALWLPGGYPELHGETLAANRAMKSSITAHVQ
AGRPVWAECGGMMALFEQLVTVDGASYPMWGVLPGNVSMQKRLAALGPQQLDVAGGTLRGHAFHYSTCATPLQAVARTRA
APGRTLRGEGEALFVAGPTSSVRASYFHAWFASCPAATAQLFGAPVSERSP

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 47381; Mature: 47249

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASTCPAILVAAPASGQGKTTVACALARLHTRQGRRVRVFKCGPDFLDPYWLTLASGAP
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHEECCCCC
VHQLDLWMTGEADCAQRLHAAAQEADLIIVEGVMGLFDGEPSAADLAQRFGLPVLAVIDA
EEEEEEEECCCHHHHHHHHHHHHHCCEEEEECHHHHCCCCCCHHHHHHHHCCCEEEEECC
SAMAGTFGALTWGLQHYREGLRFAGVLANRVASPGHAAMLEGGLRDAAQWQGALMRSEAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHC
TLPERHLGLVMSDELGDALPRLDAAADALAGTPLGQMGATELQAWSVAFEAPETLGEAFP
CCCHHHCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHEECCCHHHHHHHCC
AGALQGRTIAVARDAAFCFLYAANLDCLRALGAQLVFFSPLADTALPECDALWLPGGYPE
CCCCCCCEEEEECCCEEEEEEECCHHHHHHCCCCEEEECCHHHHCCCCCCEEECCCCCCC
LHGETLAANRAMKSSITAHVQAGRPVWAECGGMMALFEQLVTVDGASYPMWGVLPGNVSM
CCCCHHHHHHHHHHHHHEEEECCCCHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCHH
QKRLAALGPQQLDVAGGTLRGHAFHYSTCATPLQAVARTRAAPGRTLRGEGEALFVAGPT
HHHHHHCCCCCEECCCCEEECCEEECHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCC
SSVRASYFHAWFASCPAATAQLFGAPVSERSP
CHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SASTCPAILVAAPASGQGKTTVACALARLHTRQGRRVRVFKCGPDFLDPYWLTLASGAP
CCCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHEECCCCC
VHQLDLWMTGEADCAQRLHAAAQEADLIIVEGVMGLFDGEPSAADLAQRFGLPVLAVIDA
EEEEEEEECCCHHHHHHHHHHHHHCCEEEEECHHHHCCCCCCHHHHHHHHCCCEEEEECC
SAMAGTFGALTWGLQHYREGLRFAGVLANRVASPGHAAMLEGGLRDAAQWQGALMRSEAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHC
TLPERHLGLVMSDELGDALPRLDAAADALAGTPLGQMGATELQAWSVAFEAPETLGEAFP
CCCHHHCCEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHEECCCHHHHHHHCC
AGALQGRTIAVARDAAFCFLYAANLDCLRALGAQLVFFSPLADTALPECDALWLPGGYPE
CCCCCCCEEEEECCCEEEEEEECCHHHHHHCCCCEEEECCHHHHCCCCCCEEECCCCCCC
LHGETLAANRAMKSSITAHVQAGRPVWAECGGMMALFEQLVTVDGASYPMWGVLPGNVSM
CCCCHHHHHHHHHHHHHEEEECCCCHHHHHCCHHHHHHHHHHCCCCCCCEEEECCCCCHH
QKRLAALGPQQLDVAGGTLRGHAFHYSTCATPLQAVARTRAAPGRTLRGEGEALFVAGPT
HHHHHHCCCCCEECCCCEEECCEEECHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCC
SSVRASYFHAWFASCPAATAQLFGAPVSERSP
CHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]