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The map label for this gene is cbiF [H]

Identifier: 91788641

GI number: 91788641

Start: 2920557

End: 2921411

Strand: Reverse

Name: cbiF [H]

Synonym: Bpro_2779

Alternate gene names: 91788641

Gene position: 2921411-2920557 (Counterclockwise)

Preceding gene: 91788642

Following gene: 91788640

Centisome position: 56.18

GC content: 63.98

Gene sequence:

>855_bases
ATGAAAACTCTCACTTCTCATCCACAGGCTGATCGCGACACTGCTGCGCCGGGCAAGGTCTGGTTTGTCGGTGCAGGGCC
GGGCGACCCGGAACTCATCACCGTCAAGGGCCGCGCGCTGATTGAACGTGCGGGCGCCATCCTGTTTGCCGGTTCGCTGG
TCAGCGAGGCTGCCACGCGCTGGGCCCCGGTGGGTTGCGTGATTGTTGACAGCAAGGACATGACGCTGGAGCAGATGTCG
GCCTGGCTGATTGAGCAGGCTGGGCGCTGCGAAACCGTGGTACGCCTGCAAACCGGCGATCCCGGTTTGTATGGGGCGCT
GATTGAGTTAGTGCAACCGCTGGACGCTGCTGGAATCGCCATCGAGGTCGTTCCCGGTGTGTCGTCCGCCATGGCCTCCG
CTGCGGCTGCGGTGGAAAGCCTGACGCTGCCTGAGGTGACACAAACAGTGATCTTTACCCGAGTGGAAGGCCGCACGCCC
ATGCCCGAGGGCGAGTCGCTGGAGGTGCTGGCCGCGCACCACAGCACGCTGTGCATCTTCCTGAGTATTACCTTGATGGG
GCGCGTTACCGCGGCGTTGACCGTGGCGGGCTGGTCGCCGGATGCCCCCATCGTGGTGGTGCACAAGGCCAGCTGGCCCG
GCGAGGAGAAGATCGTTCGCGGCACAGTGAGCACCATCCAGAGCCTGTGCCGCGAGGCCAAAATTGTCAGCCAGGCCATG
ATCATTGCCAGCCCGACGCTGGGCGCCCGCCAGTGGACGACCCTGGCCAAGTCAAAGCTGTATGACGCCAGTTTCACGCA
TCGCTTCCGGCGCGCCAGCGTCCCAGTCTCAAACCTCATCCAGGAGACCCCATGA

Upstream 100 bases:

>100_bases
GTGTTTGACCATCGCATCACCGTGCACCAGCTCTCGGGCCAGTGGGTGACCCTGCCAAAAATCTAGCGTGTCGCCAGCAA
ACTTCAAACCCCAACACACC

Downstream 100 bases:

>100_bases
CCATGACAACCATTTTGCTGGTGGGCCACGGCTCGCGCGAGCCCGCCGGTAACGACGAGATCGAGGCCTTTACCGCGCGC
TGGCGCCTGGCCCATCCGCA

Product: precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKTLTSHPQADRDTAAPGKVWFVGAGPGDPELITVKGRALIERAGAILFAGSLVSEAATRWAPVGCVIVDSKDMTLEQMS
AWLIEQAGRCETVVRLQTGDPGLYGALIELVQPLDAAGIAIEVVPGVSSAMASAAAAVESLTLPEVTQTVIFTRVEGRTP
MPEGESLEVLAAHHSTLCIFLSITLMGRVTAALTVAGWSPDAPIVVVHKASWPGEEKIVRGTVSTIQSLCREAKIVSQAM
IIASPTLGARQWTTLAKSKLYDASFTHRFRRASVPVSNLIQETP

Sequences:

>Translated_284_residues
MKTLTSHPQADRDTAAPGKVWFVGAGPGDPELITVKGRALIERAGAILFAGSLVSEAATRWAPVGCVIVDSKDMTLEQMS
AWLIEQAGRCETVVRLQTGDPGLYGALIELVQPLDAAGIAIEVVPGVSSAMASAAAAVESLTLPEVTQTVIFTRVEGRTP
MPEGESLEVLAAHHSTLCIFLSITLMGRVTAALTVAGWSPDAPIVVVHKASWPGEEKIVRGTVSTIQSLCREAKIVSQAM
IIASPTLGARQWTTLAKSKLYDASFTHRFRRASVPVSNLIQETP
>Mature_284_residues
MKTLTSHPQADRDTAAPGKVWFVGAGPGDPELITVKGRALIERAGAILFAGSLVSEAATRWAPVGCVIVDSKDMTLEQMS
AWLIEQAGRCETVVRLQTGDPGLYGALIELVQPLDAAGIAIEVVPGVSSAMASAAAAVESLTLPEVTQTVIFTRVEGRTP
MPEGESLEVLAAHHSTLCIFLSITLMGRVTAALTVAGWSPDAPIVVVHKASWPGEEKIVRGTVSTIQSLCREAKIVSQAM
IIASPTLGARQWTTLAKSKLYDASFTHRFRRASVPVSNLIQETP

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=245, Percent_Identity=27.3469387755102, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 30076; Mature: 30076

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLTSHPQADRDTAAPGKVWFVGAGPGDPELITVKGRALIERAGAILFAGSLVSEAATR
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCHHHHHHCCHHEEEHHHHHHHHHH
WAPVGCVIVDSKDMTLEQMSAWLIEQAGRCETVVRLQTGDPGLYGALIELVQPLDAAGIA
CCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCEE
IEVVPGVSSAMASAAAAVESLTLPEVTQTVIFTRVEGRTPMPEGESLEVLAAHHSTLCIF
EEEECCCHHHHHHHHHHHHHCCCCHHHHEEEEEEECCCCCCCCCCCEEEEECCCCHHHHH
LSITLMGRVTAALTVAGWSPDAPIVVVHKASWPGEEKIVRGTVSTIQSLCREAKIVSQAM
HHHHHHHHHHEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IIASPTLGARQWTTLAKSKLYDASFTHRFRRASVPVSNLIQETP
EEECCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure
MKTLTSHPQADRDTAAPGKVWFVGAGPGDPELITVKGRALIERAGAILFAGSLVSEAATR
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCEEEECCHHHHHHCCHHEEEHHHHHHHHHH
WAPVGCVIVDSKDMTLEQMSAWLIEQAGRCETVVRLQTGDPGLYGALIELVQPLDAAGIA
CCCEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCEE
IEVVPGVSSAMASAAAAVESLTLPEVTQTVIFTRVEGRTPMPEGESLEVLAAHHSTLCIF
EEEECCCHHHHHHHHHHHHHCCCCHHHHEEEEEEECCCCCCCCCCCEEEEECCCCHHHHH
LSITLMGRVTAALTVAGWSPDAPIVVVHKASWPGEEKIVRGTVSTIQSLCREAKIVSQAM
HHHHHHHHHHEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IIASPTLGARQWTTLAKSKLYDASFTHRFRRASVPVSNLIQETP
EEECCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]