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The map label for this gene is pdhB [H]

Identifier: 91788533

GI number: 91788533

Start: 2806853

End: 2808523

Strand: Reverse

Name: pdhB [H]

Synonym: Bpro_2671

Alternate gene names: 91788533

Gene position: 2808523-2806853 (Counterclockwise)

Preceding gene: 91788534

Following gene: 91788532

Centisome position: 54.01

GC content: 64.15

Gene sequence:

>1671_bases
ATGGCAGTGGTAGATATTCAGGTTCCTGATATTGGGGACTTCGACGAAGTGACGGTGATTGAGTTGCTCGTCAAACCAGG
CGATACCGTGAAGGCTGAGCAATCCCTGATCACGGTGGAGTCCGACAAGGCCTCCATGGAGATTCCGTCCAGCCAGGCTG
GCGTGGTCAAGGAGCTCAAGGTCAAGCTCGGAGACAAGGTCAAGCAGGGTTCCATCGTGCTGACGGTGGAAGCGGCCGGC
GCCGCAGTACCGGCAACAGCATCGGCTGCGGCAGCTTCTGCTGCACCCGCTGCGAGCACGGCGGCAGCACCGGCGCCTGC
CCCCGCACCGGCGCCAGTCCCGGCGTCGGCGCCGGCCGCAGCTGCCGGCCCGGTCGAGGTCCGTGTACCCGACATCGGCG
ACTTCAAGGATGTGTCGGTCATCGAGGTGCTCGTCAAGCCGGGCGACACCATCAAGCTCGAGCAGTCCCTGGTCACCGTG
GAGTCCGACAAGGCTTCGATGGAGATTCCGTCGTCGGCCGCGGGCGTACTCAAGGAACTCAAGGTCAAGCTGGGCGACAT
CGTCAACATCGGTGATCTGCTGGCCATTCTGGAAGGCTCTGTTGCAGCCACCGGGACCTCCCCGGCGCCCGCACCGGCTG
CACCGGCCGCCGCCCCTGCACCTGTTTCTGCGGCGGCCGCCGCGCCGGCTGCTGCGGTCATGCCGGCGCATGAGCCCGCC
ACGGCCCAGCAAGGCAGTTTGCCGCATGCTTCGCCCTCGGTGCGCAAGTTCGCCCGCGAGCTCGGCGTGCCCCTGGCAGA
GGTCAAAGGCACAGGTCCGAAGGGCCGCATCACGCAGGACGACGTGCAGGCCTTCACCAAGTCGGTCATGGCGGGCGATA
CCCGCACCCAGGCGCAGGCTGCCAAGGCACCCGCATCGTCCGGTGGTACCGGCGTGGGACTGGACCTGCTGCCATGGCCC
AAGGTCGACTTCACCAAGTTCGGGCCAGTCGAGCGCAAGGACATGTCCCGCATCAAGAAGATCAGCGGCGCCAACCTGCT
TCGCAACTACGTGATGATCCCGCACGTCACCAACCATGACGATGCCGACATCACCGAGCTGGAAGCTTTCCGCGTTTCGA
CCAACAAGGAAAACGAGAAGGCAGGCCTGAAGGTCACCATGCTGGCCTTCCTGATCAAGGCCAGCGTTGCGGCGCTGAAG
AAATTCCCCGAATTCAATGCCTCGCTGGACGGCGACCAACTGGTTTACAAGCAGTACTACCACATCGGTTTTGCCGCCGA
CACGCCCAACGGGTTGGTCGTTCCCGTACTCAAGGATGCCGACAAAAAAGGCGTGCTGCAGATCAGCCAGGAAATGGGCG
AACTCGCGAAGAAGGCGCGCGACGGCAAGCTCACGCCCGCCGAAATGCAGGGCGCCAGCTTCACCATCTCGTCGCTGGGC
GGCATTGGCGGGCGTTACTTCACGCCCATCATCAACGCGCCGGAAGTCGCCATCCTCGGCGTCTCCAAGAGCCAGATGGA
ACCTGTGTGGGATGGCAAGGCCTTCCAGCCGCGCCTGATGCTGCCCATGTCGCTGGCCTGGGACCACCGCGTGATCGACG
GCGCCGGCGCCGCGCGCTTCAACGCCTATCTCGGACAAATCCTCGCCGACTTCCGCCGCGTTCTTTTATGA

Upstream 100 bases:

>100_bases
AAGCCATCAAGAAGTACGGTATCAATGCCGACAAGATCAATCCCCTTTATGCCTGATGCAGGTTTGATGCATTAGCCCGC
AAGCAATCGGAGACAACAAC

Downstream 100 bases:

>100_bases
CAACGTTGGTGGTTGTGAAGAAGGCGGGCCAGATTGCCATTGCCGCTGACACGCTGGTCACGTTTGGCGATACCAGCCTG
ACCCACCGGTTTGAGGCCAA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MAVVDIQVPDIGDFDEVTVIELLVKPGDTVKAEQSLITVESDKASMEIPSSQAGVVKELKVKLGDKVKQGSIVLTVEAAG
AAVPATASAAAASAAPAASTAAAPAPAPAPAPVPASAPAAAAGPVEVRVPDIGDFKDVSVIEVLVKPGDTIKLEQSLVTV
ESDKASMEIPSSAAGVLKELKVKLGDIVNIGDLLAILEGSVAATGTSPAPAPAAPAAAPAPVSAAAAAPAAAVMPAHEPA
TAQQGSLPHASPSVRKFARELGVPLAEVKGTGPKGRITQDDVQAFTKSVMAGDTRTQAQAAKAPASSGGTGVGLDLLPWP
KVDFTKFGPVERKDMSRIKKISGANLLRNYVMIPHVTNHDDADITELEAFRVSTNKENEKAGLKVTMLAFLIKASVAALK
KFPEFNASLDGDQLVYKQYYHIGFAADTPNGLVVPVLKDADKKGVLQISQEMGELAKKARDGKLTPAEMQGASFTISSLG
GIGGRYFTPIINAPEVAILGVSKSQMEPVWDGKAFQPRLMLPMSLAWDHRVIDGAGAARFNAYLGQILADFRRVLL

Sequences:

>Translated_556_residues
MAVVDIQVPDIGDFDEVTVIELLVKPGDTVKAEQSLITVESDKASMEIPSSQAGVVKELKVKLGDKVKQGSIVLTVEAAG
AAVPATASAAAASAAPAASTAAAPAPAPAPAPVPASAPAAAAGPVEVRVPDIGDFKDVSVIEVLVKPGDTIKLEQSLVTV
ESDKASMEIPSSAAGVLKELKVKLGDIVNIGDLLAILEGSVAATGTSPAPAPAAPAAAPAPVSAAAAAPAAAVMPAHEPA
TAQQGSLPHASPSVRKFARELGVPLAEVKGTGPKGRITQDDVQAFTKSVMAGDTRTQAQAAKAPASSGGTGVGLDLLPWP
KVDFTKFGPVERKDMSRIKKISGANLLRNYVMIPHVTNHDDADITELEAFRVSTNKENEKAGLKVTMLAFLIKASVAALK
KFPEFNASLDGDQLVYKQYYHIGFAADTPNGLVVPVLKDADKKGVLQISQEMGELAKKARDGKLTPAEMQGASFTISSLG
GIGGRYFTPIINAPEVAILGVSKSQMEPVWDGKAFQPRLMLPMSLAWDHRVIDGAGAARFNAYLGQILADFRRVLL
>Mature_555_residues
AVVDIQVPDIGDFDEVTVIELLVKPGDTVKAEQSLITVESDKASMEIPSSQAGVVKELKVKLGDKVKQGSIVLTVEAAGA
AVPATASAAAASAAPAASTAAAPAPAPAPAPVPASAPAAAAGPVEVRVPDIGDFKDVSVIEVLVKPGDTIKLEQSLVTVE
SDKASMEIPSSAAGVLKELKVKLGDIVNIGDLLAILEGSVAATGTSPAPAPAAPAAAPAPVSAAAAAPAAAVMPAHEPAT
AQQGSLPHASPSVRKFARELGVPLAEVKGTGPKGRITQDDVQAFTKSVMAGDTRTQAQAAKAPASSGGTGVGLDLLPWPK
VDFTKFGPVERKDMSRIKKISGANLLRNYVMIPHVTNHDDADITELEAFRVSTNKENEKAGLKVTMLAFLIKASVAALKK
FPEFNASLDGDQLVYKQYYHIGFAADTPNGLVVPVLKDADKKGVLQISQEMGELAKKARDGKLTPAEMQGASFTISSLGG
IGGRYFTPIINAPEVAILGVSKSQMEPVWDGKAFQPRLMLPMSLAWDHRVIDGAGAARFNAYLGQILADFRRVLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=442, Percent_Identity=30.0904977375566, Blast_Score=172, Evalue=1e-42,
Organism=Homo sapiens, GI31711992, Length=307, Percent_Identity=36.1563517915309, Blast_Score=158, Evalue=1e-38,
Organism=Homo sapiens, GI203098816, Length=479, Percent_Identity=26.9311064718163, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI203098753, Length=441, Percent_Identity=26.7573696145125, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI19923748, Length=230, Percent_Identity=33.4782608695652, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI260898739, Length=152, Percent_Identity=38.8157894736842, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1786305, Length=556, Percent_Identity=53.2374100719424, Blast_Score=506, Evalue=1e-144,
Organism=Escherichia coli, GI1786946, Length=440, Percent_Identity=31.3636363636364, Blast_Score=186, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17537937, Length=427, Percent_Identity=29.03981264637, Blast_Score=177, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI25146366, Length=434, Percent_Identity=29.4930875576037, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17560088, Length=450, Percent_Identity=29.5555555555556, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17538894, Length=310, Percent_Identity=30.6451612903226, Blast_Score=111, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320352, Length=419, Percent_Identity=30.0715990453461, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6324258, Length=434, Percent_Identity=29.7235023041475, Blast_Score=134, Evalue=3e-32,
Organism=Drosophila melanogaster, GI18859875, Length=437, Percent_Identity=31.5789473684211, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24582497, Length=222, Percent_Identity=35.5855855855856, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI20129315, Length=222, Percent_Identity=35.5855855855856, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24645909, Length=215, Percent_Identity=34.4186046511628, Blast_Score=115, Evalue=7e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 57503; Mature: 57371

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVDIQVPDIGDFDEVTVIELLVKPGDTVKAEQSLITVESDKASMEIPSSQAGVVKELK
CEEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEECCCCCEECCCCCCCHHHHHH
VKLGDKVKQGSIVLTVEAAGAAVPATASAAAASAAPAASTAAAPAPAPAPAPVPASAPAA
HHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAGPVEVRVPDIGDFKDVSVIEVLVKPGDTIKLEQSLVTVESDKASMEIPSSAAGVLKEL
CCCCEEEECCCCCCCCCCEEEEEEECCCCEEEEECEEEEEECCCCCCCCCCHHHHHHHHH
KVKLGDIVNIGDLLAILEGSVAATGTSPAPAPAAPAAAPAPVSAAAAAPAAAVMPAHEPA
HHHHCCCCCHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHCCCHHHCCCCCCCC
TAQQGSLPHASPSVRKFARELGVPLAEVKGTGPKGRITQDDVQAFTKSVMAGDTRTQAQA
CCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
AKAPASSGGTGVGLDLLPWPKVDFTKFGPVERKDMSRIKKISGANLLRNYVMIPHVTNHD
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCEEEECCCCCCC
DADITELEAFRVSTNKENEKAGLKVTMLAFLIKASVAALKKFPEFNASLDGDQLVYKQYY
CCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
HIGFAADTPNGLVVPVLKDADKKGVLQISQEMGELAKKARDGKLTPAEMQGASFTISSLG
EEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEHHHHC
GIGGRYFTPIINAPEVAILGVSKSQMEPVWDGKAFQPRLMLPMSLAWDHRVIDGAGAARF
CCCCEEECCCCCCCCEEEEECCHHHCCCCCCCCCCCCEEECEEEECCCCEEECCCCHHHH
NAYLGQILADFRRVLL
HHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AVVDIQVPDIGDFDEVTVIELLVKPGDTVKAEQSLITVESDKASMEIPSSQAGVVKELK
EEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEECCCCCEECCCCCCCHHHHHH
VKLGDKVKQGSIVLTVEAAGAAVPATASAAAASAAPAASTAAAPAPAPAPAPVPASAPAA
HHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
AAGPVEVRVPDIGDFKDVSVIEVLVKPGDTIKLEQSLVTVESDKASMEIPSSAAGVLKEL
CCCCEEEECCCCCCCCCCEEEEEEECCCCEEEEECEEEEEECCCCCCCCCCHHHHHHHHH
KVKLGDIVNIGDLLAILEGSVAATGTSPAPAPAAPAAAPAPVSAAAAAPAAAVMPAHEPA
HHHHCCCCCHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCHHHHCCCHHHCCCCCCCC
TAQQGSLPHASPSVRKFARELGVPLAEVKGTGPKGRITQDDVQAFTKSVMAGDTRTQAQA
CCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHH
AKAPASSGGTGVGLDLLPWPKVDFTKFGPVERKDMSRIKKISGANLLRNYVMIPHVTNHD
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHCEEEECCCCCCC
DADITELEAFRVSTNKENEKAGLKVTMLAFLIKASVAALKKFPEFNASLDGDQLVYKQYY
CCCHHHEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
HIGFAADTPNGLVVPVLKDADKKGVLQISQEMGELAKKARDGKLTPAEMQGASFTISSLG
EEEEEECCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCCEEEHHHHC
GIGGRYFTPIINAPEVAILGVSKSQMEPVWDGKAFQPRLMLPMSLAWDHRVIDGAGAARF
CCCCEEECCCCCCCCEEEEECCHHHCCCCCCCCCCCCEEECEEEECCCCEEECCCCHHHH
NAYLGQILADFRRVLL
HHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]