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The map label for this gene is rhtB [H]

Identifier: 91788522

GI number: 91788522

Start: 2796594

End: 2797247

Strand: Direct

Name: rhtB [H]

Synonym: Bpro_2660

Alternate gene names: 91788522

Gene position: 2796594-2797247 (Clockwise)

Preceding gene: 91788517

Following gene: 91788524

Centisome position: 53.78

GC content: 62.08

Gene sequence:

>654_bases
ATGGCCATTGACACCTGGACCGCCTATTTCCTCGCCTCCATCCTGATTGCGATTTCCCCGGGATCGGGCGCCGTCCTGGC
CATGAGCCACGGCCTGAGCTACGGCGTTAAACGCACGCAGGCCACCATCGCCGGGCTGCAGGCCGGTTTGCTGATGATCC
TGGTGGTTGCCGGCGCAGGTGTTGGCTCGCTGCTGTTGGCCTCGGAGCTGGCTTTCAACGTTGTCAAAATCCTGGGGGCC
GCCTACCTGATGTACATAGGCTGGTCACAGTGGCGTTCATCGGACGCCATCATGACGAATGATGTGGGCGCACCGGCCAC
CGAAGCGAGCGTTCCGTCATCGCGCAAACGCTGCCTCACAGGCTTGCTGACCAATGCGACCAACCCCAAAGGCATCCTCT
TCATGGTGGCCGTGCTGCCGCAGTTCATCAGCCAGGGCCGCCCGCTCTGGCTCCAGCTGCTGGTCATTGCCATAACCACG
GTGGCGGTGGACACCGTGGTGATGCATGGCTATGCCTTTGCCGCCAGCAGACTGCAACCCCTGTTTAAAAATGCCCGCGC
CATCAAGGTGCAGAACCGCCTGTTTGGCGGCCTGCTCATGGCGGTGGGTGCGGGCCTGTTTTTTGTCAAGCGCGGCCAGC
AGGCTGCGCATTAG

Upstream 100 bases:

>100_bases
CTTGACGCATGTAGGATGGCGTGCGTTTTCGCGCCTCTCCATCCTCTCCGAACCGCTTCCGTACGCGTCGTCCCTGACCC
CTCATCACCGCACAAAACCC

Downstream 100 bases:

>100_bases
GCTTTTCTTTTCATTTGTCATTAAAGCGATAGCTGCCTGCATCAGAAAATAAAGGGCTTGGCAAAAGGCTTGATGAAAGG
CTTGGCAAAAACCATGCGCA

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MAIDTWTAYFLASILIAISPGSGAVLAMSHGLSYGVKRTQATIAGLQAGLLMILVVAGAGVGSLLLASELAFNVVKILGA
AYLMYIGWSQWRSSDAIMTNDVGAPATEASVPSSRKRCLTGLLTNATNPKGILFMVAVLPQFISQGRPLWLQLLVIAITT
VAVDTVVMHGYAFAASRLQPLFKNARAIKVQNRLFGGLLMAVGAGLFFVKRGQQAAH

Sequences:

>Translated_217_residues
MAIDTWTAYFLASILIAISPGSGAVLAMSHGLSYGVKRTQATIAGLQAGLLMILVVAGAGVGSLLLASELAFNVVKILGA
AYLMYIGWSQWRSSDAIMTNDVGAPATEASVPSSRKRCLTGLLTNATNPKGILFMVAVLPQFISQGRPLWLQLLVIAITT
VAVDTVVMHGYAFAASRLQPLFKNARAIKVQNRLFGGLLMAVGAGLFFVKRGQQAAH
>Mature_216_residues
AIDTWTAYFLASILIAISPGSGAVLAMSHGLSYGVKRTQATIAGLQAGLLMILVVAGAGVGSLLLASELAFNVVKILGAA
YLMYIGWSQWRSSDAIMTNDVGAPATEASVPSSRKRCLTGLLTNATNPKGILFMVAVLPQFISQGRPLWLQLLVIAITTV
AVDTVVMHGYAFAASRLQPLFKNARAIKVQNRLFGGLLMAVGAGLFFVKRGQQAAH

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=204, Percent_Identity=41.6666666666667, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1788099, Length=146, Percent_Identity=30.1369863013699, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22876; Mature: 22745

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIDTWTAYFLASILIAISPGSGAVLAMSHGLSYGVKRTQATIAGLQAGLLMILVVAGAG
CCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VGSLLLASELAFNVVKILGAAYLMYIGWSQWRSSDAIMTNDVGAPATEASVPSSRKRCLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHH
GLLTNATNPKGILFMVAVLPQFISQGRPLWLQLLVIAITTVAVDTVVMHGYAFAASRLQP
HHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFKNARAIKVQNRLFGGLLMAVGAGLFFVKRGQQAAH
HHHCCHHEEHHHHHHHHHHHHHHCHHHEEECCCCCCC
>Mature Secondary Structure 
AIDTWTAYFLASILIAISPGSGAVLAMSHGLSYGVKRTQATIAGLQAGLLMILVVAGAG
CCCHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VGSLLLASELAFNVVKILGAAYLMYIGWSQWRSSDAIMTNDVGAPATEASVPSSRKRCLT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCHHHHHHHH
GLLTNATNPKGILFMVAVLPQFISQGRPLWLQLLVIAITTVAVDTVVMHGYAFAASRLQP
HHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFKNARAIKVQNRLFGGLLMAVGAGLFFVKRGQQAAH
HHHCCHHEEHHHHHHHHHHHHHHCHHHEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]