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The map label for this gene is pcm1

Identifier: 91788503

GI number: 91788503

Start: 2781248

End: 2781958

Strand: Reverse

Name: pcm1

Synonym: Bpro_2641

Alternate gene names: 91788503

Gene position: 2781958-2781248 (Counterclockwise)

Preceding gene: 91788505

Following gene: 91788497

Centisome position: 53.5

GC content: 61.18

Gene sequence:

>711_bases
ATGAATGTGTCCGTGCCACTTCCTTCAGGAGGCACCATGACCGAACCGGACTTCTCTGTCCTGCGCCAGTCCATGATCGC
GGAAATCGCCGCCAAGACAGCTTTTGTCAGCACGCAGTTGGGCAAAGCGGTGCTGGATCCGCGGGTCATGAACGCCATGG
CCAAGGTGCCGCGCCACGAGTTCGTTCTGCTCGAACTGCGGCCCTACGCCTACGCCGACACGCCCCTGCCAAGCTGCTTC
GACAAGACAATCTCGCAGCCGTTCATCGTCGCAGTAATGACCGACCTGCTTGAGCTGCGCCCGACCGACACGGTCCTCGA
GATCGGCACCGGGCTTGGTTACCAGACAGCCATCCTGGCGGAACTTGCGCAGCACGTTTACAGCATTGAAATCATTGAAG
AGATGGCTGTGCAGGCTCGGCAACGGCTGGCCCGACACGGTTATACCAACGTCGATATCAAGATTGGCAACGGCTGCGGC
GGGTGGCCCGAACACGCGCCGTTCGACAAGGTGATCGTCACGGCGGCGCCCGATCTGATCCCGCCACCGCTCATCTACCA
GTTGAAACCCGGCGGCAAGATGGTGATACCCGCCGGTCTGCCCAACGATCAGCAACTCATCCTGGTCGAGAAAGACGCCA
GCGATGCGGTATCGACCAGGGATATCCTGCCGGTGCGGTTCTCGCTGCTCGAGGATGCCGAGCCCGGCTGA

Upstream 100 bases:

>100_bases
TTTCTGGCCGCCAGCCGCCACTTGACTCATGCAGTGGGCTTGCGCACGCCAACTGCATGCCAAGTCAGTGCCTTGCATTT
ACCCGGGAAGCGGGCGTAGG

Downstream 100 bases:

>100_bases
TGCTGGGGCCGACAGAGCCCGGGACACCTGCCAGGTCAGCCGCCGCTGCCGCCACTGCCATAGGGACGGGTGAGTATCTC
GAGGTTGTGGCCGTCAGGGT

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 1; Protein L-isoaspartyl methyltransferase 1; Protein-beta-aspartate methyltransferase 1; PIMT 1

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MNVSVPLPSGGTMTEPDFSVLRQSMIAEIAAKTAFVSTQLGKAVLDPRVMNAMAKVPRHEFVLLELRPYAYADTPLPSCF
DKTISQPFIVAVMTDLLELRPTDTVLEIGTGLGYQTAILAELAQHVYSIEIIEEMAVQARQRLARHGYTNVDIKIGNGCG
GWPEHAPFDKVIVTAAPDLIPPPLIYQLKPGGKMVIPAGLPNDQQLILVEKDASDAVSTRDILPVRFSLLEDAEPG

Sequences:

>Translated_236_residues
MNVSVPLPSGGTMTEPDFSVLRQSMIAEIAAKTAFVSTQLGKAVLDPRVMNAMAKVPRHEFVLLELRPYAYADTPLPSCF
DKTISQPFIVAVMTDLLELRPTDTVLEIGTGLGYQTAILAELAQHVYSIEIIEEMAVQARQRLARHGYTNVDIKIGNGCG
GWPEHAPFDKVIVTAAPDLIPPPLIYQLKPGGKMVIPAGLPNDQQLILVEKDASDAVSTRDILPVRFSLLEDAEPG
>Mature_236_residues
MNVSVPLPSGGTMTEPDFSVLRQSMIAEIAAKTAFVSTQLGKAVLDPRVMNAMAKVPRHEFVLLELRPYAYADTPLPSCF
DKTISQPFIVAVMTDLLELRPTDTVLEIGTGLGYQTAILAELAQHVYSIEIIEEMAVQARQRLARHGYTNVDIKIGNGCG
GWPEHAPFDKVIVTAAPDLIPPPLIYQLKPGGKMVIPAGLPNDQQLILVEKDASDAVSTRDILPVRFSLLEDAEPG

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=175, Percent_Identity=30.2857142857143, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1789100, Length=158, Percent_Identity=50.6329113924051, Blast_Score=152, Evalue=2e-38,
Organism=Drosophila melanogaster, GI17981723, Length=178, Percent_Identity=28.6516853932584, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT1_POLSJ (Q12A85)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_549455.1
- ProteinModelPortal:   Q12A85
- SMR:   Q12A85
- STRING:   Q12A85
- GeneID:   4014712
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_2641
- NMPDR:   fig|296591.1.peg.357
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   HTIERVE
- PhylomeDB:   Q12A85
- ProtClustDB:   CLSK447965
- BioCyc:   PSP296591:BPRO_2641-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 25643; Mature: 25643

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 85-85

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSVPLPSGGTMTEPDFSVLRQSMIAEIAAKTAFVSTQLGKAVLDPRVMNAMAKVPRHE
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCE
FVLLELRPYAYADTPLPSCFDKTISQPFIVAVMTDLLELRPTDTVLEIGTGLGYQTAILA
EEEEEECCCEECCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHH
ELAQHVYSIEIIEEMAVQARQRLARHGYTNVDIKIGNGCGGWPEHAPFDKVIVTAAPDLI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCC
PPPLIYQLKPGGKMVIPAGLPNDQQLILVEKDASDAVSTRDILPVRFSLLEDAEPG
CCCEEEEECCCCCEEEECCCCCCCEEEEEECCCCCCHHHHCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MNVSVPLPSGGTMTEPDFSVLRQSMIAEIAAKTAFVSTQLGKAVLDPRVMNAMAKVPRHE
CCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCE
FVLLELRPYAYADTPLPSCFDKTISQPFIVAVMTDLLELRPTDTVLEIGTGLGYQTAILA
EEEEEECCCEECCCCCCHHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHH
ELAQHVYSIEIIEEMAVQARQRLARHGYTNVDIKIGNGCGGWPEHAPFDKVIVTAAPDLI
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCC
PPPLIYQLKPGGKMVIPAGLPNDQQLILVEKDASDAVSTRDILPVRFSLLEDAEPG
CCCEEEEECCCCCEEEECCCCCCCEEEEEECCCCCCHHHHCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA