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The map label for this gene is yfkN [H]

Identifier: 91788487

GI number: 91788487

Start: 2758886

End: 2760610

Strand: Reverse

Name: yfkN [H]

Synonym: Bpro_2625

Alternate gene names: 91788487

Gene position: 2760610-2758886 (Counterclockwise)

Preceding gene: 91788488

Following gene: 91788486

Centisome position: 53.09

GC content: 61.51

Gene sequence:

>1725_bases
ATGAATCTGTCCCGCCGCGAATTTGCAAGTGTCATGGCTGCGGCCCTGGCTGCCGGTTTTCCGTTACAGCGCGAGGCCAG
TGCTGCCCAGGCTGGTGGCTTGTACAACTTGCCGCGCTCCGGCAATGTGCACCTGTTGCACATGACGGATTGCCACGCCC
AGTTGTTGCCCAGTTATTTTCGCGAGCCCTCTGTCAACCTGGGCGTCGGCGCCATGCGCGGGCAGGTTCCTCATCTGGTG
GGCGAGCAACTGTTGAAAGCGGCCAGCCTGAAGTCGGGCACACCCGAGGCGCATGCCTTTACCTATCTGGACTTTGAGCG
GTCTGCCCGCCAGTACGGCAAGGTGGGTGGTTTTTCCCACCTGGCTACGCTGGTCAGGCAACTCAAGGCGGATCGCCCCG
GTGCGCTGCTGCTCGATGGCGGTGATACCTGGCAAGGCAGCGCCACGGCGCTCTGGACGCAGGGCCAGGACATGGTGGAC
GCCGCGAAGTTGCTGGGCGTGGACATCATGACCGGCCACTGGGACTTCACCTATGGCGAGGAACGTGTCAAGAAAATCAT
CGAGCAGGACTTTGCCGGCAAGGTCGACTTCATCGCGCAAAACATCAAGACCAGCGACTTTGGCGACCCGGTGTTCAAGC
CCTATGTGATCAGGGAGATGAACGGCGTGGCTGTCGCCATCGTGGGCCAGGCCTTTCCCTACACACCCATTGCCAACCCC
GGCTGGCAAACGCCAAACTGGACTTTCGGCATCCGCGAAAGCGAGATGCAGGAAACGGTCAATGAGGCGCGCGCCAAGGG
CGCGCAGGCGGTGGTGCTGCTCAGCCACAACGGCATGGACGTGGACCTGAAGATGGCCTCGCGCGTTACCGGTATCGACG
CCATTCTGGGTGGGCATACTCACGACGGCGTGCCGGTGCCTGTGATTGTCAAAAACCCGGGCGGCCAGACCATCGTGACC
AATGCGGGAAGCAATGGCAAGTTTCTGGGCGTGCTGGATCTGGACGTGAAGGACAAGCAGGTGCGGGGTTTCAGCTACCG
CCTGCTGCCGGTGTTTTCCAGTCTGTTGCCGGCTGATTCCGCCATGAGTGCGCTGATTTCCAGGGTGCGTGCGCCTTATG
ATGCCAAATTGTCGGAAAAACTGGCGTTGACCGAAGGCACGCTGTACCGCCGTGGCAATTTCAACGGCACCTTTGACCAG
CTCATCCTCGACGCCCTGATGGACGTACGCGGCGCCGAAATCGCCTTTTCCCCCGGGTTTCGCTGGGGAACATCGTTGCT
GCCCGGCCAGGCCATCACGCGCGAACACCTGATGGACCAGACCGCGATCACCTATCCGTGGACCACCCTGACGGACATGC
GCGGCGAGACGATCAAAACCATTCTGGAGGACGTGGGTGACAACCTGTTCAATCCCGATCCTTATTACCAGCAGGGCGGT
GACATGGTGCGGGTGGGCGGGCTGACCTATACCTGCGAGCCGGGAGCCAAAATGGGGTCGCGCATCCAGAACATGCGCCT
GAAAGGCCAATTGATTGAGGCTGGCAAGACTTACAAGGTGGCCGGCTGGGCGCCGGTTGCCGAGGCCAGCAAACATGCCG
GGCCGCCCGTCTGGGAGGTGGTCGAGCACTATCTCAAGGCTCAAAAGGTGATCAAACCGGCCAAGCCCAATACCCCCGAG
CTGCTCGGCGTTGCGGGTAACCCCGGGCTCGGCCTGGCGCTGTAA

Upstream 100 bases:

>100_bases
GGCGATTCTGGCTGTCTGCCCGGGAAATAGGGGCGCAAACAGCTCAAATTGATAGTAGTTTCAACCCACACCCGGCTTGC
CGGGCAGGATGCCTGACGCC

Downstream 100 bases:

>100_bases
CCCGGGGCTCGGCCCCTGGAGCTTTCTCAGGGGCTGTGGTCATGCTGGTGCGGTAAAACACCCGCAATACCAGCGGTATC
GGCCCAAAAATCGGACCGGA

Product: sulfate thiol esterase SoxB

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 574; Mature: 574

Protein sequence:

>574_residues
MNLSRREFASVMAAALAAGFPLQREASAAQAGGLYNLPRSGNVHLLHMTDCHAQLLPSYFREPSVNLGVGAMRGQVPHLV
GEQLLKAASLKSGTPEAHAFTYLDFERSARQYGKVGGFSHLATLVRQLKADRPGALLLDGGDTWQGSATALWTQGQDMVD
AAKLLGVDIMTGHWDFTYGEERVKKIIEQDFAGKVDFIAQNIKTSDFGDPVFKPYVIREMNGVAVAIVGQAFPYTPIANP
GWQTPNWTFGIRESEMQETVNEARAKGAQAVVLLSHNGMDVDLKMASRVTGIDAILGGHTHDGVPVPVIVKNPGGQTIVT
NAGSNGKFLGVLDLDVKDKQVRGFSYRLLPVFSSLLPADSAMSALISRVRAPYDAKLSEKLALTEGTLYRRGNFNGTFDQ
LILDALMDVRGAEIAFSPGFRWGTSLLPGQAITREHLMDQTAITYPWTTLTDMRGETIKTILEDVGDNLFNPDPYYQQGG
DMVRVGGLTYTCEPGAKMGSRIQNMRLKGQLIEAGKTYKVAGWAPVAEASKHAGPPVWEVVEHYLKAQKVIKPAKPNTPE
LLGVAGNPGLGLAL

Sequences:

>Translated_574_residues
MNLSRREFASVMAAALAAGFPLQREASAAQAGGLYNLPRSGNVHLLHMTDCHAQLLPSYFREPSVNLGVGAMRGQVPHLV
GEQLLKAASLKSGTPEAHAFTYLDFERSARQYGKVGGFSHLATLVRQLKADRPGALLLDGGDTWQGSATALWTQGQDMVD
AAKLLGVDIMTGHWDFTYGEERVKKIIEQDFAGKVDFIAQNIKTSDFGDPVFKPYVIREMNGVAVAIVGQAFPYTPIANP
GWQTPNWTFGIRESEMQETVNEARAKGAQAVVLLSHNGMDVDLKMASRVTGIDAILGGHTHDGVPVPVIVKNPGGQTIVT
NAGSNGKFLGVLDLDVKDKQVRGFSYRLLPVFSSLLPADSAMSALISRVRAPYDAKLSEKLALTEGTLYRRGNFNGTFDQ
LILDALMDVRGAEIAFSPGFRWGTSLLPGQAITREHLMDQTAITYPWTTLTDMRGETIKTILEDVGDNLFNPDPYYQQGG
DMVRVGGLTYTCEPGAKMGSRIQNMRLKGQLIEAGKTYKVAGWAPVAEASKHAGPPVWEVVEHYLKAQKVIKPAKPNTPE
LLGVAGNPGLGLAL
>Mature_574_residues
MNLSRREFASVMAAALAAGFPLQREASAAQAGGLYNLPRSGNVHLLHMTDCHAQLLPSYFREPSVNLGVGAMRGQVPHLV
GEQLLKAASLKSGTPEAHAFTYLDFERSARQYGKVGGFSHLATLVRQLKADRPGALLLDGGDTWQGSATALWTQGQDMVD
AAKLLGVDIMTGHWDFTYGEERVKKIIEQDFAGKVDFIAQNIKTSDFGDPVFKPYVIREMNGVAVAIVGQAFPYTPIANP
GWQTPNWTFGIRESEMQETVNEARAKGAQAVVLLSHNGMDVDLKMASRVTGIDAILGGHTHDGVPVPVIVKNPGGQTIVT
NAGSNGKFLGVLDLDVKDKQVRGFSYRLLPVFSSLLPADSAMSALISRVRAPYDAKLSEKLALTEGTLYRRGNFNGTFDQ
LILDALMDVRGAEIAFSPGFRWGTSLLPGQAITREHLMDQTAITYPWTTLTDMRGETIKTILEDVGDNLFNPDPYYQQGG
DMVRVGGLTYTCEPGAKMGSRIQNMRLKGQLIEAGKTYKVAGWAPVAEASKHAGPPVWEVVEHYLKAQKVIKPAKPNTPE
LLGVAGNPGLGLAL

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=505, Percent_Identity=23.960396039604, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1790658, Length=230, Percent_Identity=25.2173913043478, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921980, Length=421, Percent_Identity=22.0902612826603, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI161076508, Length=421, Percent_Identity=21.6152019002375, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24652512, Length=421, Percent_Identity=21.6152019002375, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 62115; Mature: 62115

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLSRREFASVMAAALAAGFPLQREASAAQAGGLYNLPRSGNVHLLHMTDCHAQLLPSYF
CCCCHHHHHHHHHHHHHHCCCCCHHCCHHHCCCCEECCCCCCEEEEEECCCHHHHHHHHH
REPSVNLGVGAMRGQVPHLVGEQLLKAASLKSGTPEAHAFTYLDFERSARQYGKVGGFSH
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHCCCCHHH
LATLVRQLKADRPGALLLDGGDTWQGSATALWTQGQDMVDAAKLLGVDIMTGHWDFTYGE
HHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHCCEEEECCCCCCCCH
ERVKKIIEQDFAGKVDFIAQNIKTSDFGDPVFKPYVIREMNGVAVAIVGQAFPYTPIANP
HHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCC
GWQTPNWTFGIRESEMQETVNEARAKGAQAVVLLSHNGMDVDLKMASRVTGIDAILGGHT
CCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEHHHHHCCHHHHHCCCC
HDGVPVPVIVKNPGGQTIVTNAGSNGKFLGVLDLDVKDKQVRGFSYRLLPVFSSLLPADS
CCCCCEEEEEECCCCCEEEEECCCCCCEEEEEECCCCCCHHCCCEEEHHHHHHHHCCCHH
AMSALISRVRAPYDAKLSEKLALTEGTLYRRGNFNGTFDQLILDALMDVRGAEIAFSPGF
HHHHHHHHHCCCCCCCHHHHEECCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCC
RWGTSLLPGQAITREHLMDQTAITYPWTTLTDMRGETIKTILEDVGDNLFNPDPYYQQGG
CCCCCCCCCHHHHHHHHHCCCEEECCCHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHCCC
DMVRVGGLTYTCEPGAKMGSRIQNMRLKGQLIEAGKTYKVAGWAPVAEASKHAGPPVWEV
CEEEECCEEEEECCCHHHHHHHHHHHHHHEEECCCCEEEEECCCCHHHHHHCCCCCHHHH
VEHYLKAQKVIKPAKPNTPELLGVAGNPGLGLAL
HHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCC
>Mature Secondary Structure
MNLSRREFASVMAAALAAGFPLQREASAAQAGGLYNLPRSGNVHLLHMTDCHAQLLPSYF
CCCCHHHHHHHHHHHHHHCCCCCHHCCHHHCCCCEECCCCCCEEEEEECCCHHHHHHHHH
REPSVNLGVGAMRGQVPHLVGEQLLKAASLKSGTPEAHAFTYLDFERSARQYGKVGGFSH
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHCCCCHHH
LATLVRQLKADRPGALLLDGGDTWQGSATALWTQGQDMVDAAKLLGVDIMTGHWDFTYGE
HHHHHHHHHCCCCCEEEEECCCCCCCCEEEEEECCCHHHHHHHHHCCEEEECCCCCCCCH
ERVKKIIEQDFAGKVDFIAQNIKTSDFGDPVFKPYVIREMNGVAVAIVGQAFPYTPIANP
HHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCC
GWQTPNWTFGIRESEMQETVNEARAKGAQAVVLLSHNGMDVDLKMASRVTGIDAILGGHT
CCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEHHHHHCCHHHHHCCCC
HDGVPVPVIVKNPGGQTIVTNAGSNGKFLGVLDLDVKDKQVRGFSYRLLPVFSSLLPADS
CCCCCEEEEEECCCCCEEEEECCCCCCEEEEEECCCCCCHHCCCEEEHHHHHHHHCCCHH
AMSALISRVRAPYDAKLSEKLALTEGTLYRRGNFNGTFDQLILDALMDVRGAEIAFSPGF
HHHHHHHHHCCCCCCCHHHHEECCCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCC
RWGTSLLPGQAITREHLMDQTAITYPWTTLTDMRGETIKTILEDVGDNLFNPDPYYQQGG
CCCCCCCCCHHHHHHHHHCCCEEECCCHHHHHCCCHHHHHHHHHHCCCCCCCCCHHHCCC
DMVRVGGLTYTCEPGAKMGSRIQNMRLKGQLIEAGKTYKVAGWAPVAEASKHAGPPVWEV
CEEEECCEEEEECCCHHHHHHHHHHHHHHEEECCCCEEEEECCCCHHHHHHCCCCCHHHH
VEHYLKAQKVIKPAKPNTPELLGVAGNPGLGLAL
HHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]