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The map label for this gene is rluB [H]

Identifier: 91788475

GI number: 91788475

Start: 2743464

End: 2745098

Strand: Reverse

Name: rluB [H]

Synonym: Bpro_2613

Alternate gene names: 91788475

Gene position: 2745098-2743464 (Counterclockwise)

Preceding gene: 91788476

Following gene: 91788474

Centisome position: 52.79

GC content: 65.32

Gene sequence:

>1635_bases
ATGAATCAACCAGACCACCCGGCTTCCGACCTCCCGCCCACTCAGCCGGGCGAAGATGAAAATAAACCTGCTGCGACAGA
AAAATCCGGGCAGGACGCCCCTGATTTAATAGCTGATAAGCCCCAAGAAAAGGCCGTGGCGTTAGCGCAGCCCGCCGGAC
AGCCGGTGGCGCAGCCTGCGACCAGCACTATCCGTTTCGAGGACGTGGTGACCGGGCAGTTTGACGCTGATGAAGACGAT
GTGGCGCTCGAGTTGCCCAAGCGGGTGCTGTCGCCTCAGCCGGAAACCCCCAAGCTGCACAAGGTGCTGGCGCAAGCCGG
GTTGGGCTCCCGCCTGGAGATGGAGCAGCTTATTCTCGAGGGACGCATTTCGGTCAATGCCGAGCCGGCTCACATCGGGC
AGCGCATCCAGTTCGGCGACACCGTCAAGGTCAACGGTAAGCCCATTCGCGTGCGAATTGATCCGCCGCCGCCTCGCGTG
ATTGCGTATCACAAGCCCAGCGGAGAGGTTGTGACGCACGACGATCCGCAAAACCGGCCCACGGTGTTCCGGCGCTTGCC
CAAATTGTTTCAGGGTAAATGGCAGTCCGTCGGGCGTCTTGACCTGAACACCGAAGGCTTGCTCTTGTTGACGAGTTCGG
GCGAGCTGGCCAACAAGCTGATGCATCCGCGCTTTGGCCTAGAGCGCGAGTATGCGGTGCGGGTGTTGGGTGCGCTGAGC
AACGAGGAGAAAAAGCGCCTGCTGGAAGGCGTGACCCTGGATGATGGCGTGGCGCAGTTCAGCTCCATCGAGGCCGGTGG
CGGCGAGGGTGCCAATTGCTGGTACCGGGTCACCATATCCGAGGGGCGCAATCGTGAGGTTCGCCGCATGTTCGAGGCGG
TGGGCCACGCGGTCAGCCGTCTCATTCGGATCCGCTACGGCGCCATGCTGTTGCCGCGCGGACTGAAGCGGGGCGCATTC
GTCGAACTGGATGAACGGGATATCTGGGCGCTGACCAATGCGGTCACCCGCTCCGAGTCCCGGGCGGATCCGCGGGGCGC
GCCCAAACACCCGGGTCATGTTCGCGGCGACGAAGGTGGCGAATTCCGCCCAGGCCGCGGCCCTCGCCGAAATGAGCGCT
CGCGCGGTGACCGCGGGCGCCTGCCGGCCCCCGAGAGCGGGCAAGCCGATGACTTTCAGCCCGCCGATGCCGATGCCGAG
CAGGAGCCCAATGAGCTGCAGCCGCCAATGCACCGGACGGACTCTGCGCCCAGGGGCAACAGGCGCCGCGGGGGGAAAGG
TCGTGGGCAGGGGGGTAACCCCGGTTCACCCCCTCGCTTTGGACAGGCTGCGGGAAATGGTCCGCAGGGACCAAAGCGGC
GCGATGACCGTGGGGACCGGGGTGACAAAAGCGCCGGGGGGGCCCAGCAGCCCGATCCGATGAAGACATCCTTTGGTTAT
ATCGGTGCGGATACCTTCACCCGGCAGCGGCAAGGTCAAGGTCAAGGTCAAGGTCAGGGGCAACGCCGCAGTGGCGGCGG
GGGATCTGGCATGGGCGGTGCCGGTGGGGGGCAGCGCCGTGGCGGCGGGGGTGGTGGCGGAAACCGCGGAGGCGGCAATC
GTGGTGGTGGCGGTAATCGCGGCGGAAATCGTTGA

Upstream 100 bases:

>100_bases
CCGGAAGTCCTGGAAGCTTCTGCGGCGAATGCCGGTGAGTTTTCCCAATCTGAGCCCCGTGACCGGACCGACAGCGAACC
TTACGCATGAATGTAAAAGC

Downstream 100 bases:

>100_bases
GCGCGCCAGAAGCACTTTACTTCACAGGGTTGATTCGAACAGATCAGCCCACAGGTTAAAATCAGAGGCTGTATTCAGTT
TCTGAATCATTTAAAAATCC

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 544; Mature: 544

Protein sequence:

>544_residues
MNQPDHPASDLPPTQPGEDENKPAATEKSGQDAPDLIADKPQEKAVALAQPAGQPVAQPATSTIRFEDVVTGQFDADEDD
VALELPKRVLSPQPETPKLHKVLAQAGLGSRLEMEQLILEGRISVNAEPAHIGQRIQFGDTVKVNGKPIRVRIDPPPPRV
IAYHKPSGEVVTHDDPQNRPTVFRRLPKLFQGKWQSVGRLDLNTEGLLLLTSSGELANKLMHPRFGLEREYAVRVLGALS
NEEKKRLLEGVTLDDGVAQFSSIEAGGGEGANCWYRVTISEGRNREVRRMFEAVGHAVSRLIRIRYGAMLLPRGLKRGAF
VELDERDIWALTNAVTRSESRADPRGAPKHPGHVRGDEGGEFRPGRGPRRNERSRGDRGRLPAPESGQADDFQPADADAE
QEPNELQPPMHRTDSAPRGNRRRGGKGRGQGGNPGSPPRFGQAAGNGPQGPKRRDDRGDRGDKSAGGAQQPDPMKTSFGY
IGADTFTRQRQGQGQGQGQGQRRSGGGGSGMGGAGGGQRRGGGGGGGNRGGGNRGGGGNRGGNR

Sequences:

>Translated_544_residues
MNQPDHPASDLPPTQPGEDENKPAATEKSGQDAPDLIADKPQEKAVALAQPAGQPVAQPATSTIRFEDVVTGQFDADEDD
VALELPKRVLSPQPETPKLHKVLAQAGLGSRLEMEQLILEGRISVNAEPAHIGQRIQFGDTVKVNGKPIRVRIDPPPPRV
IAYHKPSGEVVTHDDPQNRPTVFRRLPKLFQGKWQSVGRLDLNTEGLLLLTSSGELANKLMHPRFGLEREYAVRVLGALS
NEEKKRLLEGVTLDDGVAQFSSIEAGGGEGANCWYRVTISEGRNREVRRMFEAVGHAVSRLIRIRYGAMLLPRGLKRGAF
VELDERDIWALTNAVTRSESRADPRGAPKHPGHVRGDEGGEFRPGRGPRRNERSRGDRGRLPAPESGQADDFQPADADAE
QEPNELQPPMHRTDSAPRGNRRRGGKGRGQGGNPGSPPRFGQAAGNGPQGPKRRDDRGDRGDKSAGGAQQPDPMKTSFGY
IGADTFTRQRQGQGQGQGQGQRRSGGGGSGMGGAGGGQRRGGGGGGGNRGGGNRGGGGNRGGNR
>Mature_544_residues
MNQPDHPASDLPPTQPGEDENKPAATEKSGQDAPDLIADKPQEKAVALAQPAGQPVAQPATSTIRFEDVVTGQFDADEDD
VALELPKRVLSPQPETPKLHKVLAQAGLGSRLEMEQLILEGRISVNAEPAHIGQRIQFGDTVKVNGKPIRVRIDPPPPRV
IAYHKPSGEVVTHDDPQNRPTVFRRLPKLFQGKWQSVGRLDLNTEGLLLLTSSGELANKLMHPRFGLEREYAVRVLGALS
NEEKKRLLEGVTLDDGVAQFSSIEAGGGEGANCWYRVTISEGRNREVRRMFEAVGHAVSRLIRIRYGAMLLPRGLKRGAF
VELDERDIWALTNAVTRSESRADPRGAPKHPGHVRGDEGGEFRPGRGPRRNERSRGDRGRLPAPESGQADDFQPADADAE
QEPNELQPPMHRTDSAPRGNRRRGGKGRGQGGNPGSPPRFGQAAGNGPQGPKRRDDRGDRGDKSAGGAQQPDPMKTSFGY
IGADTFTRQRQGQGQGQGQGQRRSGGGGSGMGGAGGGQRRGGGGGGGNRGGGNRGGGGNRGGNR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=232, Percent_Identity=54.7413793103448, Blast_Score=242, Evalue=4e-65,
Organism=Escherichia coli, GI1790453, Length=249, Percent_Identity=28.9156626506024, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI1788510, Length=173, Percent_Identity=33.5260115606936, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI87081838, Length=170, Percent_Identity=33.5294117647059, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 58215; Mature: 58215

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQPDHPASDLPPTQPGEDENKPAATEKSGQDAPDLIADKPQEKAVALAQPAGQPVAQPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHEEHHCCCCCCCCCCC
TSTIRFEDVVTGQFDADEDDVALELPKRVLSPQPETPKLHKVLAQAGLGSRLEMEQLILE
CCEEEEEHEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
GRISVNAEPAHIGQRIQFGDTVKVNGKPIRVRIDPPPPRVIAYHKPSGEVVTHDDPQNRP
CCCCCCCCHHHHCCEEECCCEEEECCEEEEEEECCCCCCEEEEECCCCCEEECCCCCCCC
TVFRRLPKLFQGKWQSVGRLDLNTEGLLLLTSSGELANKLMHPRFGLEREYAVRVLGALS
HHHHHHHHHHHHHHHHCCEEECCCCCEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHC
NEEKKRLLEGVTLDDGVAQFSSIEAGGGEGANCWYRVTISEGRNREVRRMFEAVGHAVSR
CHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
LIRIRYGAMLLPRGLKRGAFVELDERDIWALTNAVTRSESRADPRGAPKHPGHVRGDEGG
HHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
EFRPGRGPRRNERSRGDRGRLPAPESGQADDFQPADADAEQEPNELQPPMHRTDSAPRGN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCC
RRRGGKGRGQGGNPGSPPRFGQAAGNGPQGPKRRDDRGDRGDKSAGGAQQPDPMKTSFGY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCC
IGADTFTRQRQGQGQGQGQGQRRSGGGGSGMGGAGGGQRRGGGGGGGNRGGGNRGGGGNR
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGNR
CCCC
>Mature Secondary Structure
MNQPDHPASDLPPTQPGEDENKPAATEKSGQDAPDLIADKPQEKAVALAQPAGQPVAQPA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCHHHHEEHHCCCCCCCCCCC
TSTIRFEDVVTGQFDADEDDVALELPKRVLSPQPETPKLHKVLAQAGLGSRLEMEQLILE
CCEEEEEHEEECCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHH
GRISVNAEPAHIGQRIQFGDTVKVNGKPIRVRIDPPPPRVIAYHKPSGEVVTHDDPQNRP
CCCCCCCCHHHHCCEEECCCEEEECCEEEEEEECCCCCCEEEEECCCCCEEECCCCCCCC
TVFRRLPKLFQGKWQSVGRLDLNTEGLLLLTSSGELANKLMHPRFGLEREYAVRVLGALS
HHHHHHHHHHHHHHHHCCEEECCCCCEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHC
NEEKKRLLEGVTLDDGVAQFSSIEAGGGEGANCWYRVTISEGRNREVRRMFEAVGHAVSR
CHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHH
LIRIRYGAMLLPRGLKRGAFVELDERDIWALTNAVTRSESRADPRGAPKHPGHVRGDEGG
HHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
EFRPGRGPRRNERSRGDRGRLPAPESGQADDFQPADADAEQEPNELQPPMHRTDSAPRGN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCC
RRRGGKGRGQGGNPGSPPRFGQAAGNGPQGPKRRDDRGDRGDKSAGGAQQPDPMKTSFGY
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCC
IGADTFTRQRQGQGQGQGQGQRRSGGGGSGMGGAGGGQRRGGGGGGGNRGGGNRGGGGNR
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GGNR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]