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The map label for this gene is rlmN [H]

Identifier: 91788473

GI number: 91788473

Start: 2741693

End: 2742841

Strand: Reverse

Name: rlmN [H]

Synonym: Bpro_2611

Alternate gene names: 91788473

Gene position: 2742841-2741693 (Counterclockwise)

Preceding gene: 91788474

Following gene: 91788472

Centisome position: 52.74

GC content: 59.79

Gene sequence:

>1149_bases
ATGACAGCCAACCTTCTTGACTACGATCTGGAGGGCTTGGCCGCCTTTTGCGAAGGGCTCGGCGAAAAGCGCTTTCGCGC
AACCCAGCTGTTTCGCTGGATTCATCAAAAAGGCGCCTCTGATTTTGGGCAGATGACAGATCTGGCCAGATCGCTGCGTG
AAAAACTCGCCGGTTCGGCCCATATCCAGGGGCCGAAAGTGGTGTCCCGTCACGACTCGGCAGACGGCACCATCAAATGG
CTGTTCGATGTTGGCGCCGGTGACGTCATTGAAGCCGTTTTCATCCCCGAAACGGATCGGGGAACCCTGTGCATTTCATC
CCAGGCCGGCTGCGCCGTGGGGTGCCGGTTCTGCTCGACCGGGCACCAGGGCTTCAGCCGCAACCTCACTACGGGGGAAA
TTGTCTCCCAGCTGTGGTTTGCAGAGCATTTCCTGCGCCAGCACCTGGGCCGGCAGGAGCGTGTGATTTCGAATGTGGTC
ATGATGGGCATGGGCGAGCCCTTGCAGAACTACTCGCAACTGGTACCCGCGTTGCGGGTCATGCTGGATGACCATGGTTA
CGGTTTGTCGCGTCGCCGTGTGACAGTGTCGACGTCGGGCGTCGTGCCCATGATCGACAGGCTGGCCAAGGACTGTCCGG
TGGCGCTGGCTGTGTCGTTGCACGCTCCGCAAGACGCGCTGCGCGACAGCCTGGTGCCACTCAATAAAAAATACCCGATC
GCTGAATTGCTGCAGGCCTGCACCCGCTATCAGGCGTCTGCGCCGCGTGACTTCATCACGTTTGAGTACTGCATGCTTGA
CGGGGTGAATGACCAGCCCGAACATGCGCGCCAGCTGGTGGCGCTGATGCAAAATCATTCGGCAGGTGGCCTGTCGTGCA
AATTCAACCTGATTCCTTTCAATCCGTTTCCGGCGTCGGGCCTGAAGCGTTCAGCCATGCCGCAGGTTGCCGCCTTCGCC
AAAATCCTGATGGATGCGGGCATCGTGACCACGGTGCGTAAGACCCGCGGTGATGACATTGACGCGGCCTGTGGTCAACT
CGCTGGCGATGTTCAGGACCGGACCAGCGTAGACCGGCGCATCGCGGCGCAACGGCAAGGTATGCTCGGCGGCATCAAAG
TGGTTGTTGCAAAGGGGATGGATGTATGA

Upstream 100 bases:

>100_bases
ATGAATATTTACTCGCGTTAATCATGCTGCCCCCCATGCGTTGCTACTCCCCGCCAGGGTCGTCGCTTGCCTGGGGCGGT
CCAGCCTGTCGCCCTCTGCA

Downstream 100 bases:

>100_bases
GGTTTTCCGGCAATGTGATCTGCATGTCAGCCTGCTGGGTGGCTGTGTTACTGGCAGGGTGTGCCGGCCAGCAAGGAATG
TCCGGGCCCTCCGGTAACCG

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 382; Mature: 381

Protein sequence:

>382_residues
MTANLLDYDLEGLAAFCEGLGEKRFRATQLFRWIHQKGASDFGQMTDLARSLREKLAGSAHIQGPKVVSRHDSADGTIKW
LFDVGAGDVIEAVFIPETDRGTLCISSQAGCAVGCRFCSTGHQGFSRNLTTGEIVSQLWFAEHFLRQHLGRQERVISNVV
MMGMGEPLQNYSQLVPALRVMLDDHGYGLSRRRVTVSTSGVVPMIDRLAKDCPVALAVSLHAPQDALRDSLVPLNKKYPI
AELLQACTRYQASAPRDFITFEYCMLDGVNDQPEHARQLVALMQNHSAGGLSCKFNLIPFNPFPASGLKRSAMPQVAAFA
KILMDAGIVTTVRKTRGDDIDAACGQLAGDVQDRTSVDRRIAAQRQGMLGGIKVVVAKGMDV

Sequences:

>Translated_382_residues
MTANLLDYDLEGLAAFCEGLGEKRFRATQLFRWIHQKGASDFGQMTDLARSLREKLAGSAHIQGPKVVSRHDSADGTIKW
LFDVGAGDVIEAVFIPETDRGTLCISSQAGCAVGCRFCSTGHQGFSRNLTTGEIVSQLWFAEHFLRQHLGRQERVISNVV
MMGMGEPLQNYSQLVPALRVMLDDHGYGLSRRRVTVSTSGVVPMIDRLAKDCPVALAVSLHAPQDALRDSLVPLNKKYPI
AELLQACTRYQASAPRDFITFEYCMLDGVNDQPEHARQLVALMQNHSAGGLSCKFNLIPFNPFPASGLKRSAMPQVAAFA
KILMDAGIVTTVRKTRGDDIDAACGQLAGDVQDRTSVDRRIAAQRQGMLGGIKVVVAKGMDV
>Mature_381_residues
TANLLDYDLEGLAAFCEGLGEKRFRATQLFRWIHQKGASDFGQMTDLARSLREKLAGSAHIQGPKVVSRHDSADGTIKWL
FDVGAGDVIEAVFIPETDRGTLCISSQAGCAVGCRFCSTGHQGFSRNLTTGEIVSQLWFAEHFLRQHLGRQERVISNVVM
MGMGEPLQNYSQLVPALRVMLDDHGYGLSRRRVTVSTSGVVPMIDRLAKDCPVALAVSLHAPQDALRDSLVPLNKKYPIA
ELLQACTRYQASAPRDFITFEYCMLDGVNDQPEHARQLVALMQNHSAGGLSCKFNLIPFNPFPASGLKRSAMPQVAAFAK
ILMDAGIVTTVRKTRGDDIDAACGQLAGDVQDRTSVDRRIAAQRQGMLGGIKVVVAKGMDV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=374, Percent_Identity=51.6042780748663, Blast_Score=379, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41678; Mature: 41547

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANLLDYDLEGLAAFCEGLGEKRFRATQLFRWIHQKGASDFGQMTDLARSLREKLAGSA
CCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
HIQGPKVVSRHDSADGTIKWLFDVGAGDVIEAVFIPETDRGTLCISSQAGCAVGCRFCST
CCCCCCHHCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHC
GHQGFSRNLTTGEIVSQLWFAEHFLRQHLGRQERVISNVVMMGMGEPLQNYSQLVPALRV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
MLDDHGYGLSRRRVTVSTSGVVPMIDRLAKDCPVALAVSLHAPQDALRDSLVPLNKKYPI
HHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCCCCCCH
AELLQACTRYQASAPRDFITFEYCMLDGVNDQPEHARQLVALMQNHSAGGLSCKFNLIPF
HHHHHHHHHHHCCCCCCHHEEHHHEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEC
NPFPASGLKRSAMPQVAAFAKILMDAGIVTTVRKTRGDDIDAACGQLAGDVQDRTSVDRR
CCCCCCCCCHHCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHH
IAAQRQGMLGGIKVVVAKGMDV
HHHHHCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
TANLLDYDLEGLAAFCEGLGEKRFRATQLFRWIHQKGASDFGQMTDLARSLREKLAGSA
CCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCC
HIQGPKVVSRHDSADGTIKWLFDVGAGDVIEAVFIPETDRGTLCISSQAGCAVGCRFCST
CCCCCCHHCCCCCCCCEEEEEEECCCCCCEEEEECCCCCCCCEEEECCCCHHHHHHHHHC
GHQGFSRNLTTGEIVSQLWFAEHFLRQHLGRQERVISNVVMMGMGEPLQNYSQLVPALRV
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
MLDDHGYGLSRRRVTVSTSGVVPMIDRLAKDCPVALAVSLHAPQDALRDSLVPLNKKYPI
HHCCCCCCCCCCEEEEECCCCHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCCCCCCH
AELLQACTRYQASAPRDFITFEYCMLDGVNDQPEHARQLVALMQNHSAGGLSCKFNLIPF
HHHHHHHHHHHCCCCCCHHEEHHHEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEC
NPFPASGLKRSAMPQVAAFAKILMDAGIVTTVRKTRGDDIDAACGQLAGDVQDRTSVDRR
CCCCCCCCCHHCCHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHH
IAAQRQGMLGGIKVVVAKGMDV
HHHHHCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA