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The map label for this gene is purA [H]

Identifier: 91788459

GI number: 91788459

Start: 2727108

End: 2728448

Strand: Reverse

Name: purA [H]

Synonym: Bpro_2597

Alternate gene names: 91788459

Gene position: 2728448-2727108 (Counterclockwise)

Preceding gene: 91788460

Following gene: 91788458

Centisome position: 52.47

GC content: 61.52

Gene sequence:

>1341_bases
ATGACATATAAACCAACCGTAGCCGCGGGCCGCAATGTTGTGGTCGTGGGCACCCAGTGGGGCGACGAAGGCAAGGGCAA
GCTGGTCGACTGGCTTACCGAAATGTCCCAGGGTGTGGTGCGCTTTCAGGGTGGCCACAATGCCGGCCATACACTGGTCA
TCAACGGCATCAAGACTGCCTTGCACCTGATTCCCAGCGGCATCATGCGTCCTGGTGTGAAATGCTACATCGGCAACGGC
GTGGTGCTCTCGGCGGCCAAGCTGTTTGAAGAAATTGAAGGGCTGGAAAAAGCCGGCGTGGAAGTGCGCTCACGTTTGCG
CATCAGCGAAGCCTGCCCGCTGATCCTGCCTTTTCATGCAGCGCTCGACATTGCCCGCGAGGCTTACCGCGAAAAGGGCG
GTACCGCCAGGATAGGCACCACCGGCCGCGGTATTGGCCCGGCCTACGAAGACAAAATCGCGCGCCGCGCGCTGCGCGTG
CAGGACCTCAAATACCCCGAGCGTTTTGCCGCCAAGCTGCGCGAGTTGCTGGATTTGCACAACTTTGTGCTGACCGGTTA
TTTGCACGCGCCGGCGATTGATTTTGACACCGTGTATGCCGAAGCCATGCGGCACGCCGAACTCCTCAAGCCAATGATGG
CGGATGTCTCGCGCGAACTCAACGATGCGAACCGCGAAGGTGCCAATCTGCTGTTCGAGGGCGCGCAGGGCACCCTGCTC
GATGTGGACCACGGTACCTATCCCTACGTGACATCCAGCAACTGCGTGGCGGGCAATGCCGCTGCCGGCGCGGGTGTGGG
CCCGGGCATGCTGCACTACATCCTGGGCATCACCAAGGCTTATTGCACGCGCGTGGGCGGTGGGCCTTTCCCGACCGAGC
TGGATTGGGAAACGCCGGACACCGTGGGCTACCACCTCAGCACCGTGGGCGCCGAAAAAGGGGTGACCACTGGTCGTAGC
CGACGCTGCGGGTGGTTTGACGCTGCGCTGCTCAAGCGCTCCGCCCAGGTCAATGGCCTGTCGGGTCTGTGTATCACCAA
GCTTGACGTGCTCGACGGCATTGCCGAGCTCAAGCTGTGCACCGGTTATGAACTCGATGGCCACATTACCGACATCCTGC
CCATGGGCGCCGAAGACATCGAGCGTTGCAAGCCCATTTATGAAACCCTGCCGGGCTGGAGCCAGAGCACGGTGGGCGTC
ACGCAATACGACCAGCTGCCGGCTGCGGCGCAGAAATATCTCCACCGGATCGAAGAGGTGACCGGTGTGCCGATTGACAT
GATTTCCACCAGCCCGGATCGCGACCATACCATCCTGCTGCGCAATCCCTACGCCGCCTGA

Upstream 100 bases:

>100_bases
CAATGGGTGGTCCAGCCCTTGAAAAAGGCGGCCTGAAGCCGGGCCTCAGGCCGGCCTTCAATGCTGTTTTTCCCAACGAA
TCCTTAACGAGAAATTGAAG

Downstream 100 bases:

>100_bases
CCCCGGTAATTTTTGAATCAAAAAAACAGGCGCCCGGACCATGCATTGCGGCGCCTGGCTATTAAATCAGGAGCAATCCC
ATGTTGACCGAAGACGGCAA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MTYKPTVAAGRNVVVVGTQWGDEGKGKLVDWLTEMSQGVVRFQGGHNAGHTLVINGIKTALHLIPSGIMRPGVKCYIGNG
VVLSAAKLFEEIEGLEKAGVEVRSRLRISEACPLILPFHAALDIAREAYREKGGTARIGTTGRGIGPAYEDKIARRALRV
QDLKYPERFAAKLRELLDLHNFVLTGYLHAPAIDFDTVYAEAMRHAELLKPMMADVSRELNDANREGANLLFEGAQGTLL
DVDHGTYPYVTSSNCVAGNAAAGAGVGPGMLHYILGITKAYCTRVGGGPFPTELDWETPDTVGYHLSTVGAEKGVTTGRS
RRCGWFDAALLKRSAQVNGLSGLCITKLDVLDGIAELKLCTGYELDGHITDILPMGAEDIERCKPIYETLPGWSQSTVGV
TQYDQLPAAAQKYLHRIEEVTGVPIDMISTSPDRDHTILLRNPYAA

Sequences:

>Translated_446_residues
MTYKPTVAAGRNVVVVGTQWGDEGKGKLVDWLTEMSQGVVRFQGGHNAGHTLVINGIKTALHLIPSGIMRPGVKCYIGNG
VVLSAAKLFEEIEGLEKAGVEVRSRLRISEACPLILPFHAALDIAREAYREKGGTARIGTTGRGIGPAYEDKIARRALRV
QDLKYPERFAAKLRELLDLHNFVLTGYLHAPAIDFDTVYAEAMRHAELLKPMMADVSRELNDANREGANLLFEGAQGTLL
DVDHGTYPYVTSSNCVAGNAAAGAGVGPGMLHYILGITKAYCTRVGGGPFPTELDWETPDTVGYHLSTVGAEKGVTTGRS
RRCGWFDAALLKRSAQVNGLSGLCITKLDVLDGIAELKLCTGYELDGHITDILPMGAEDIERCKPIYETLPGWSQSTVGV
TQYDQLPAAAQKYLHRIEEVTGVPIDMISTSPDRDHTILLRNPYAA
>Mature_445_residues
TYKPTVAAGRNVVVVGTQWGDEGKGKLVDWLTEMSQGVVRFQGGHNAGHTLVINGIKTALHLIPSGIMRPGVKCYIGNGV
VLSAAKLFEEIEGLEKAGVEVRSRLRISEACPLILPFHAALDIAREAYREKGGTARIGTTGRGIGPAYEDKIARRALRVQ
DLKYPERFAAKLRELLDLHNFVLTGYLHAPAIDFDTVYAEAMRHAELLKPMMADVSRELNDANREGANLLFEGAQGTLLD
VDHGTYPYVTSSNCVAGNAAAGAGVGPGMLHYILGITKAYCTRVGGGPFPTELDWETPDTVGYHLSTVGAEKGVTTGRSR
RCGWFDAALLKRSAQVNGLSGLCITKLDVLDGIAELKLCTGYELDGHITDILPMGAEDIERCKPIYETLPGWSQSTVGVT
QYDQLPAAAQKYLHRIEEVTGVPIDMISTSPDRDHTILLRNPYAA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI34577063, Length=439, Percent_Identity=40.3189066059225, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI22748717, Length=433, Percent_Identity=40.4157043879908, Blast_Score=310, Evalue=1e-84,
Organism=Homo sapiens, GI40316944, Length=401, Percent_Identity=39.1521197007481, Blast_Score=276, Evalue=2e-74,
Organism=Escherichia coli, GI1790620, Length=438, Percent_Identity=59.3607305936073, Blast_Score=512, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI25146553, Length=434, Percent_Identity=40.7834101382488, Blast_Score=307, Evalue=8e-84,
Organism=Caenorhabditis elegans, GI25146555, Length=434, Percent_Identity=40.7834101382488, Blast_Score=306, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6324109, Length=434, Percent_Identity=42.8571428571429, Blast_Score=333, Evalue=3e-92,
Organism=Drosophila melanogaster, GI21356033, Length=431, Percent_Identity=41.2993039443155, Blast_Score=302, Evalue=4e-82,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 48271; Mature: 48140

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYKPTVAAGRNVVVVGTQWGDEGKGKLVDWLTEMSQGVVRFQGGHNAGHTLVINGIKTA
CCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCEEEEEHHHHH
LHLIPSGIMRPGVKCYIGNGVVLSAAKLFEEIEGLEKAGVEVRSRLRISEACPLILPFHA
HHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEHHHH
ALDIAREAYREKGGTARIGTTGRGIGPAYEDKIARRALRVQDLKYPERFAAKLRELLDLH
HHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NFVLTGYLHAPAIDFDTVYAEAMRHAELLKPMMADVSRELNDANREGANLLFEGAQGTLL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEE
DVDHGTYPYVTSSNCVAGNAAAGAGVGPGMLHYILGITKAYCTRVGGGPFPTELDWETPD
ECCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TVGYHLSTVGAEKGVTTGRSRRCGWFDAALLKRSAQVNGLSGLCITKLDVLDGIAELKLC
CCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
TGYELDGHITDILPMGAEDIERCKPIYETLPGWSQSTVGVTQYDQLPAAAQKYLHRIEEV
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHH
TGVPIDMISTSPDRDHTILLRNPYAA
CCCCEEEECCCCCCCCEEEECCCCCC
>Mature Secondary Structure 
TYKPTVAAGRNVVVVGTQWGDEGKGKLVDWLTEMSQGVVRFQGGHNAGHTLVINGIKTA
CCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCEEEEECCCCCCCEEEEEHHHHH
LHLIPSGIMRPGVKCYIGNGVVLSAAKLFEEIEGLEKAGVEVRSRLRISEACPLILPFHA
HHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCEEEHHHH
ALDIAREAYREKGGTARIGTTGRGIGPAYEDKIARRALRVQDLKYPERFAAKLRELLDLH
HHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NFVLTGYLHAPAIDFDTVYAEAMRHAELLKPMMADVSRELNDANREGANLLFEGAQGTLL
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEE
DVDHGTYPYVTSSNCVAGNAAAGAGVGPGMLHYILGITKAYCTRVGGGPFPTELDWETPD
ECCCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TVGYHLSTVGAEKGVTTGRSRRCGWFDAALLKRSAQVNGLSGLCITKLDVLDGIAELKLC
CCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
TGYELDGHITDILPMGAEDIERCKPIYETLPGWSQSTVGVTQYDQLPAAAQKYLHRIEEV
CCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHH
TGVPIDMISTSPDRDHTILLRNPYAA
CCCCEEEECCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA