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The map label for this gene is ligA

Identifier: 91788454

GI number: 91788454

Start: 2717310

End: 2719430

Strand: Reverse

Name: ligA

Synonym: Bpro_2592

Alternate gene names: 91788454

Gene position: 2719430-2717310 (Counterclockwise)

Preceding gene: 91788455

Following gene: 91788453

Centisome position: 52.29

GC content: 61.53

Gene sequence:

>2121_bases
ATGGCCACCAAAGACCTGTTTGCGCAGCCGGAGCCTTCGCGGCAGCCGGCGTCCCCTGCTGAACGCATGGCCGCCTTGCG
CGCTGAACTTCACGCTCATGCTCATCGCTATTACGTCCTGGACGAGCCGACCATTCCCGACAGCGAGTACGACAGGCTGT
TCAAGGAGTTGCAGGACCTGGAAGCGGCATACCCGGAGTTGCTGACACCGGATTCCCCGACGCAGCGCGTGGGCGGCAAG
CCGCTTGACCAGTTTGCCAGCGTGCGCCACAAGGTGCCCATGCTCAGCATTCGCACGGAGACGGACACAGAGGCCACCGG
CGCGCAAAATTTCGACACCCGCGTGCGCAAGGAACTGGGGCTGACGGAGTCGAATCCGCCGGTCGAGTATGTGGCTGAAC
TGAAGTTTGACGGGCTGGCCATGAGCTTGCGTTATGAGCACGGTGTGCTGGTGCAGGCGGCCACCCGCGGCGACGGCGAG
GTCGGGGAAGAGGTGACGCAGAACATCCGTACCATCAGGCAAATTCCTCTCAAACTGCCATCAGATGCACCACCTGTGCT
GGAAGTGCGGGGTGAGGTTTATATGCGGCGCGCTGATTTCGAAGCCTTGAACGACAAACAGCGAGAAAAAATCGCGGCGG
GCGCCAAAGGTGAAAAAACCTTTGTGAATCCGCGCAACGCCGCCGCTGGTGCGGTGCGCCAGCTGGATCCCGCTATCGCC
GCGCAGCGCCCGTTGAGCTTTTTTGCCTATGGCGTGGGCGAGGTGGTGCTGGAGGCCGAACAAGCCAACCCGAAGAACCC
TTGGGTTGCGACCCATATGGAGCTTCTGCAAGCGCTGAAGTCATGGGGGTTTCCAGTGGCAGCCCAGACAAAAATTGCGC
ATGGTGCTTCTGAATTGATAGCGTTTCACCAGGCCATGGGCCAGCAGCGCGACAGCCTGCCTTACGACATTGATGGGGTG
GTGTACAAGGTCAACAGCCTGGCATTGCAGAAACAGATGGGGTTTGTCAGCCGGGAACCACGCTGGGCTGTGGCCCATAA
ATACCCGGCGCAGGAACAGCTCACCACCGTGCTGGGCATTGAGGTGCAGGTCGGGCGCACGGGTAAGTTGACGCCGGTGG
CCAAGCTGGCACCGGTCTTTGTGGGTGGCGTTACCGTGACCAATGCCACGCTGCACAACGAGGACGAAGCGCGCCGCAAG
GATGTGCGGGTGGGCGACACCGTGATTGTTCGCCGTGCCGGCGACGTGATCCCCGAGGTTGTCAGCGTGCTGCCCGACAA
GCGATTGCCGGGCGCGCCGATGTTCACGATGCCGCGCCAATGTCCGGTCTGCGGCTCCGATGCGGTGCGCGAGGAGGGCG
AAGCCGATTACCGGTGCACCGGCGGGCTTTTTTGCGGCGCACAGCGCAAGGAAGCCATCCTGCACTATGCGCACCGACGC
GCCGTCGAGATTGAGGGCCTGGGTGACAAGCTGGTGGAGCAATTGGTGGACGCCAATGTGATTCGCACATTACCCGACCT
TTACAAGCTGGGCCTGACGGCTTTGGCCAGCCTGGACCGCATGGCCGAAAAATCGGCCAATAACCTGCTCAAGGCGCTGG
AAAAATCCAAGCAGACCACCTTGCCGCGCTTTCTGTTCGGCCTGGGTATCCGCCACGTGGGTGAAGCCACAGCCAAGGAA
CTGGCGCGTCATTTTGGCAATCTGGACGCCATCATGGATGCCACGCAAGAGCAGTTGCTGGCTGTCAGCGACGTCGGCTC
CATCGTTGCCCAGAGCATACGTACCTTCTTTGACCAGCCCCACAACCGTGAGGTGGTCGAGCAGCTGCGCGCCTGTGGCG
TTCACTGGGAGGAGGGAGAACCTGCTGCCGTTGCCCCCAAACCGCTGTCCGGGAAGACCTTTGTGATCACCGGCACCTTG
CCCACGCTGAGCCGGGATGAGGCCAAGGACAAGGTGGAGGCCGCCGGTGGCAAGGTAGCTGGCTCGGTCAGCAAAAAGAC
GGATTATGTGGTGGCAGGTACCGAGGCTGGCAGCAAGCTGGTCAAGGCGCAGGAGCTTGGTATCGCCGTGATCGATGAAG
CGGCCTTGATGCGCCTGCTGGCTTCTGCTGAAGCTGAATAA

Upstream 100 bases:

>100_bases
TGACACGCTGGACGGCCGCGACCTGTCGGCCGGCTCTGTGCTGGGCAGGCGTCTGTTTTCCTGACTTTCCCGGGCTTGCC
GCTCACTTTTGACCTTTTTC

Downstream 100 bases:

>100_bases
AGCGTAATTTATCGCTGCATTCTCTCTGCGGCCACGCCGTAAGTAGTCCTCGCCGCTTACAGATCATTACAGGGCTTTGC
AAACCCGACGTCACGCCACG

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 706; Mature: 705

Protein sequence:

>706_residues
MATKDLFAQPEPSRQPASPAERMAALRAELHAHAHRYYVLDEPTIPDSEYDRLFKELQDLEAAYPELLTPDSPTQRVGGK
PLDQFASVRHKVPMLSIRTETDTEATGAQNFDTRVRKELGLTESNPPVEYVAELKFDGLAMSLRYEHGVLVQAATRGDGE
VGEEVTQNIRTIRQIPLKLPSDAPPVLEVRGEVYMRRADFEALNDKQREKIAAGAKGEKTFVNPRNAAAGAVRQLDPAIA
AQRPLSFFAYGVGEVVLEAEQANPKNPWVATHMELLQALKSWGFPVAAQTKIAHGASELIAFHQAMGQQRDSLPYDIDGV
VYKVNSLALQKQMGFVSREPRWAVAHKYPAQEQLTTVLGIEVQVGRTGKLTPVAKLAPVFVGGVTVTNATLHNEDEARRK
DVRVGDTVIVRRAGDVIPEVVSVLPDKRLPGAPMFTMPRQCPVCGSDAVREEGEADYRCTGGLFCGAQRKEAILHYAHRR
AVEIEGLGDKLVEQLVDANVIRTLPDLYKLGLTALASLDRMAEKSANNLLKALEKSKQTTLPRFLFGLGIRHVGEATAKE
LARHFGNLDAIMDATQEQLLAVSDVGSIVAQSIRTFFDQPHNREVVEQLRACGVHWEEGEPAAVAPKPLSGKTFVITGTL
PTLSRDEAKDKVEAAGGKVAGSVSKKTDYVVAGTEAGSKLVKAQELGIAVIDEAALMRLLASAEAE

Sequences:

>Translated_706_residues
MATKDLFAQPEPSRQPASPAERMAALRAELHAHAHRYYVLDEPTIPDSEYDRLFKELQDLEAAYPELLTPDSPTQRVGGK
PLDQFASVRHKVPMLSIRTETDTEATGAQNFDTRVRKELGLTESNPPVEYVAELKFDGLAMSLRYEHGVLVQAATRGDGE
VGEEVTQNIRTIRQIPLKLPSDAPPVLEVRGEVYMRRADFEALNDKQREKIAAGAKGEKTFVNPRNAAAGAVRQLDPAIA
AQRPLSFFAYGVGEVVLEAEQANPKNPWVATHMELLQALKSWGFPVAAQTKIAHGASELIAFHQAMGQQRDSLPYDIDGV
VYKVNSLALQKQMGFVSREPRWAVAHKYPAQEQLTTVLGIEVQVGRTGKLTPVAKLAPVFVGGVTVTNATLHNEDEARRK
DVRVGDTVIVRRAGDVIPEVVSVLPDKRLPGAPMFTMPRQCPVCGSDAVREEGEADYRCTGGLFCGAQRKEAILHYAHRR
AVEIEGLGDKLVEQLVDANVIRTLPDLYKLGLTALASLDRMAEKSANNLLKALEKSKQTTLPRFLFGLGIRHVGEATAKE
LARHFGNLDAIMDATQEQLLAVSDVGSIVAQSIRTFFDQPHNREVVEQLRACGVHWEEGEPAAVAPKPLSGKTFVITGTL
PTLSRDEAKDKVEAAGGKVAGSVSKKTDYVVAGTEAGSKLVKAQELGIAVIDEAALMRLLASAEAE
>Mature_705_residues
ATKDLFAQPEPSRQPASPAERMAALRAELHAHAHRYYVLDEPTIPDSEYDRLFKELQDLEAAYPELLTPDSPTQRVGGKP
LDQFASVRHKVPMLSIRTETDTEATGAQNFDTRVRKELGLTESNPPVEYVAELKFDGLAMSLRYEHGVLVQAATRGDGEV
GEEVTQNIRTIRQIPLKLPSDAPPVLEVRGEVYMRRADFEALNDKQREKIAAGAKGEKTFVNPRNAAAGAVRQLDPAIAA
QRPLSFFAYGVGEVVLEAEQANPKNPWVATHMELLQALKSWGFPVAAQTKIAHGASELIAFHQAMGQQRDSLPYDIDGVV
YKVNSLALQKQMGFVSREPRWAVAHKYPAQEQLTTVLGIEVQVGRTGKLTPVAKLAPVFVGGVTVTNATLHNEDEARRKD
VRVGDTVIVRRAGDVIPEVVSVLPDKRLPGAPMFTMPRQCPVCGSDAVREEGEADYRCTGGLFCGAQRKEAILHYAHRRA
VEIEGLGDKLVEQLVDANVIRTLPDLYKLGLTALASLDRMAEKSANNLLKALEKSKQTTLPRFLFGLGIRHVGEATAKEL
ARHFGNLDAIMDATQEQLLAVSDVGSIVAQSIRTFFDQPHNREVVEQLRACGVHWEEGEPAAVAPKPLSGKTFVITGTLP
TLSRDEAKDKVEAAGGKVAGSVSKKTDYVVAGTEAGSKLVKAQELGIAVIDEAALMRLLASAEAE

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=73, Percent_Identity=53.4246575342466, Blast_Score=74, Evalue=6e-13,
Organism=Escherichia coli, GI1788750, Length=692, Percent_Identity=52.8901734104046, Blast_Score=704, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=414, Percent_Identity=24.8792270531401, Blast_Score=82, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_POLSJ (Q12AD4)

Other databases:

- EMBL:   CP000316
- RefSeq:   YP_549406.1
- ProteinModelPortal:   Q12AD4
- STRING:   Q12AD4
- GeneID:   4014741
- GenomeReviews:   CP000316_GR
- KEGG:   pol:Bpro_2592
- NMPDR:   fig|296591.1.peg.305
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q12AD4
- ProtClustDB:   CLSK2776430
- BioCyc:   PSP296591:BPRO_2592-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 76987; Mature: 76855

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 135-135 BINDING 133-133 BINDING 156-156 BINDING 192-192 BINDING 323-323 BINDING 347-347

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKDLFAQPEPSRQPASPAERMAALRAELHAHAHRYYVLDEPTIPDSEYDRLFKELQDL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHH
EAAYPELLTPDSPTQRVGGKPLDQFASVRHKVPMLSIRTETDTEATGAQNFDTRVRKELG
HHHCHHHCCCCCCHHHCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHC
LTESNPPVEYVAELKFDGLAMSLRYEHGVLVQAATRGDGEVGEEVTQNIRTIRQIPLKLP
CCCCCCCHHHHHHHCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCC
SDAPPVLEVRGEVYMRRADFEALNDKQREKIAAGAKGEKTFVNPRNAAAGAVRQLDPAIA
CCCCCHHEECCHHHEEHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCHHHH
AQRPLSFFAYGVGEVVLEAEQANPKNPWVATHMELLQALKSWGFPVAAQTKIAHGASELI
HHCCHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHCCHHHHH
AFHQAMGQQRDSLPYDIDGVVYKVNSLALQKQMGFVSREPRWAVAHKYPAQEQLTTVLGI
HHHHHHCCCCCCCCCCCCHHEEHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHEE
EVQVGRTGKLTPVAKLAPVFVGGVTVTNATLHNEDEARRKDVRVGDTVIVRRAGDVIPEV
EEEECCCCCCCHHHHHHHHHCCCEEEEECEECCCHHHHHHCCCCCCEEEEEHHHHHHHHH
VSVLPDKRLPGAPMFTMPRQCPVCGSDAVREEGEADYRCTGGLFCGAQRKEAILHYAHRR
HHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEECCCEEECCHHHHHHHHHHHHH
AVEIEGLGDKLVEQLVDANVIRTLPDLYKLGLTALASLDRMAEKSANNLLKALEKSKQTT
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPRFLFGLGIRHVGEATAKELARHFGNLDAIMDATQEQLLAVSDVGSIVAQSIRTFFDQP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HNREVVEQLRACGVHWEEGEPAAVAPKPLSGKTFVITGTLPTLSRDEAKDKVEAAGGKVA
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEE
GSVSKKTDYVVAGTEAGSKLVKAQELGIAVIDEAALMRLLASAEAE
CCCCCCCCEEEEECCCCHHHHHHHHCCCCEECHHHHHHHHHHCCCC
>Mature Secondary Structure 
ATKDLFAQPEPSRQPASPAERMAALRAELHAHAHRYYVLDEPTIPDSEYDRLFKELQDL
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHH
EAAYPELLTPDSPTQRVGGKPLDQFASVRHKVPMLSIRTETDTEATGAQNFDTRVRKELG
HHHCHHHCCCCCCHHHCCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHC
LTESNPPVEYVAELKFDGLAMSLRYEHGVLVQAATRGDGEVGEEVTQNIRTIRQIPLKLP
CCCCCCCHHHHHHHCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCC
SDAPPVLEVRGEVYMRRADFEALNDKQREKIAAGAKGEKTFVNPRNAAAGAVRQLDPAIA
CCCCCHHEECCHHHEEHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCHHHH
AQRPLSFFAYGVGEVVLEAEQANPKNPWVATHMELLQALKSWGFPVAAQTKIAHGASELI
HHCCHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHCCHHHHH
AFHQAMGQQRDSLPYDIDGVVYKVNSLALQKQMGFVSREPRWAVAHKYPAQEQLTTVLGI
HHHHHHCCCCCCCCCCCCHHEEHHHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHEE
EVQVGRTGKLTPVAKLAPVFVGGVTVTNATLHNEDEARRKDVRVGDTVIVRRAGDVIPEV
EEEECCCCCCCHHHHHHHHHCCCEEEEECEECCCHHHHHHCCCCCCEEEEEHHHHHHHHH
VSVLPDKRLPGAPMFTMPRQCPVCGSDAVREEGEADYRCTGGLFCGAQRKEAILHYAHRR
HHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEEECCCEEECCHHHHHHHHHHHHH
AVEIEGLGDKLVEQLVDANVIRTLPDLYKLGLTALASLDRMAEKSANNLLKALEKSKQTT
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LPRFLFGLGIRHVGEATAKELARHFGNLDAIMDATQEQLLAVSDVGSIVAQSIRTFFDQP
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
HNREVVEQLRACGVHWEEGEPAAVAPKPLSGKTFVITGTLPTLSRDEAKDKVEAAGGKVA
CCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEE
GSVSKKTDYVVAGTEAGSKLVKAQELGIAVIDEAALMRLLASAEAE
CCCCCCCCEEEEECCCCHHHHHHHHCCCCEECHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA