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The map label for this gene is ogt [H]

Identifier: 91788422

GI number: 91788422

Start: 2681450

End: 2682070

Strand: Reverse

Name: ogt [H]

Synonym: Bpro_2560

Alternate gene names: 91788422

Gene position: 2682070-2681450 (Counterclockwise)

Preceding gene: 91788423

Following gene: 91788421

Centisome position: 51.58

GC content: 67.15

Gene sequence:

>621_bases
ATGAGCACCGAAGGCTTCGCGCTGTTTGACACTCCCCTTGGCCACTGCGGCATTGCCTGGGGCGACCGTGGGCTGGTGGG
CGTGCTATTGCCCGAGCACAGCCTGGCGCAGACTCGCGCTCGCATGAAGCGCTTGTATCCGCAGATGCATGAAATGCCTC
CTGTGCCCGAAGCCCAGGCCGCGATCACCCGCATAACGGCCCTCCTGAACGGCGAGCGGGACGACCTGAGCGACATCGCG
CTGGACATGAGCGAAGTGCCGCCATTTCATCAGCGGGTGTACGAGGTCGCTCGCGCCATTCCGCCGGGCCGCACGCTCAC
CTATGGTGAAGTAGCCGCCCGGATCGGTGAGCCGGGCGCTGCCCGCGCTGTGGGGCAGGCCTTGGGTCACAACCCCTTCG
CGCCCGTCGTGCCCTGCCACCGTGTGCTGGCCGTGGGCGCCCGCTCGGGAGGCTTCTCGGCCGGCGGCGGCGTGGCAACC
AAGCTGCGCATGCTGCAGATTGAGGGCGCGCAGATTGGCGACGCGCCTGGCTTGTTTGACGGTGACGGGCCGCTCCAGCA
AGTGCGCAATTCGCCAGACACTCTTGCCGACAAGCCCCGGATGGGCCAACAACGCAAATAG

Upstream 100 bases:

>100_bases
GCGTGGATATGAGGCTGGCAACACACATATACTTTTTCCTGGCTGTGCGTTCGGGCGCATTCGGATGCGATCGGGTGCAG
GGGCTTCCATCATCTGATTC

Downstream 100 bases:

>100_bases
GCACTGCGCACCGCCTGCCTCGAAAACACGGTGGTATCGAGATGGGTACAGAATGCGATTACGGGCGGACACCGCAGCCC
TGCGCTATCCTCGCGTTTGT

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MSTEGFALFDTPLGHCGIAWGDRGLVGVLLPEHSLAQTRARMKRLYPQMHEMPPVPEAQAAITRITALLNGERDDLSDIA
LDMSEVPPFHQRVYEVARAIPPGRTLTYGEVAARIGEPGAARAVGQALGHNPFAPVVPCHRVLAVGARSGGFSAGGGVAT
KLRMLQIEGAQIGDAPGLFDGDGPLQQVRNSPDTLADKPRMGQQRK

Sequences:

>Translated_206_residues
MSTEGFALFDTPLGHCGIAWGDRGLVGVLLPEHSLAQTRARMKRLYPQMHEMPPVPEAQAAITRITALLNGERDDLSDIA
LDMSEVPPFHQRVYEVARAIPPGRTLTYGEVAARIGEPGAARAVGQALGHNPFAPVVPCHRVLAVGARSGGFSAGGGVAT
KLRMLQIEGAQIGDAPGLFDGDGPLQQVRNSPDTLADKPRMGQQRK
>Mature_205_residues
STEGFALFDTPLGHCGIAWGDRGLVGVLLPEHSLAQTRARMKRLYPQMHEMPPVPEAQAAITRITALLNGERDDLSDIAL
DMSEVPPFHQRVYEVARAIPPGRTLTYGEVAARIGEPGAARAVGQALGHNPFAPVVPCHRVLAVGARSGGFSAGGGVATK
LRMLQIEGAQIGDAPGLFDGDGPLQQVRNSPDTLADKPRMGQQRK

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=164, Percent_Identity=32.3170731707317, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787596, Length=82, Percent_Identity=46.3414634146341, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=127, Percent_Identity=33.8582677165354, Blast_Score=71, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI115533068, Length=169, Percent_Identity=30.1775147928994, Blast_Score=71, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 21915; Mature: 21784

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEGFALFDTPLGHCGIAWGDRGLVGVLLPEHSLAQTRARMKRLYPQMHEMPPVPEAQA
CCCCCCEEECCCCCCCEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AITRITALLNGERDDLSDIALDMSEVPPFHQRVYEVARAIPPGRTLTYGEVAARIGEPGA
HHHHHHHHHCCCCCCHHHHHHCHHHCCHHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCHH
ARAVGQALGHNPFAPVVPCHRVLAVGARSGGFSAGGGVATKLRMLQIEGAQIGDAPGLFD
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHEEEEEECCCCCCCCCCCCC
GDGPLQQVRNSPDTLADKPRMGQQRK
CCCHHHHHCCCCCHHCCCCCCCCCCC
>Mature Secondary Structure 
STEGFALFDTPLGHCGIAWGDRGLVGVLLPEHSLAQTRARMKRLYPQMHEMPPVPEAQA
CCCCCEEECCCCCCCEECCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AITRITALLNGERDDLSDIALDMSEVPPFHQRVYEVARAIPPGRTLTYGEVAARIGEPGA
HHHHHHHHHCCCCCCHHHHHHCHHHCCHHHHHHHHHHHCCCCCCCEEHHHHHHHHCCCHH
ARAVGQALGHNPFAPVVPCHRVLAVGARSGGFSAGGGVATKLRMLQIEGAQIGDAPGLFD
HHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHEEEEEECCCCCCCCCCCCC
GDGPLQQVRNSPDTLADKPRMGQQRK
CCCHHHHHCCCCCHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]