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The map label for this gene is ywbI [H]

Identifier: 91788309

GI number: 91788309

Start: 2547532

End: 2548425

Strand: Direct

Name: ywbI [H]

Synonym: Bpro_2444

Alternate gene names: 91788309

Gene position: 2547532-2548425 (Clockwise)

Preceding gene: 91788307

Following gene: 91788310

Centisome position: 48.99

GC content: 65.88

Gene sequence:

>894_bases
ATGCGCTTCGATCTGACCGACCTGCGGCTCTTCCTGAACGTTCATGAGGCGGGAACGATCACGGGCGGGGCAGGGCGCAG
CCACATGACGCTGGCTTCGGCAAGCGAACGCATCCGCAGCATGGAGGACACCCTGGGCGTGCCCCTGCTGGTGCGCGAGC
GCCGCGGCGTGGCGGTCACGGCGGCAGGCCGCACATTGCTGTACCACGCGCGCGTGGTGTTGCAGCAACTGGAGCGCATG
CGCGGAGAACTGGGTGATTACGGCGCCGGGCTCAAGGGGCATATCCGCCTGCTCTGCAATACGGCGGCCCTGAGCGAGTA
CTTGCCGCAGGCGCTCAGCCGTTTTCTCGCGAAATATCCGCAGATATCGGTCGATCTGGAAGAAAGGCCGAGCCAGGAGA
TCGTCGACGCTGTGCGGGCCGGGCTCTGCGACATGGGCGTGGTGTCGGACCTTGTGGACCTTCAAGGGCTGCAGGCCTTC
AAATTCCGCTCCGATCCGCTGGTGCTCGTGGTGCCTGAGGGCCATGTGCTGGCCGGCCGCCGCACCATCAGACTGTCCGA
AGTGCTGGATTGCGACTTTGTCGGCCTCGTCGATGGCAGCGCCTTGCAGGAACATGTGACCCAGCAGGCCCGTCGGCTGG
GTGGGCAGCTAAGTTACCGCGTGCGCTTGCGCAGTTTCGAGGCGGTATGCCGCATGGTGGGGCTGGGCATCGGCGTTGGC
ATCGTCCCGCGGGCGGCGGCTGCCCGATGCGCGCGTTCCGCCAGACTCAAGCGAGTGACGCTCACGGATGCCTGGGCCAA
CCGGAGCCTGGTGCTATGCCTGCGTGACGTGGACCAATTGCCGCTTTACGCGCGACAAATGGTGCAGCACGTGCTGGCCG
CCGAATCGCCGTAA

Upstream 100 bases:

>100_bases
TGAGCGCCCATTTTGTTGAAGGCAGCACCAGGTGAAAATTCGGAAATGGCTGAGCCAGCCTCAGGCGGCTCCTGAGTCTT
CTTGTGTTAACTTCATTGGC

Downstream 100 bases:

>100_bases
AAAACGGTGCGGCAAGCCATACATGCCATTTTTCGCCTATGCTTTGAGACATGTACTTGTCCCCCCAATTCAAGGGCGAC
CGCGCGCAGGCCGCGGCGCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRFDLTDLRLFLNVHEAGTITGGAGRSHMTLASASERIRSMEDTLGVPLLVRERRGVAVTAAGRTLLYHARVVLQQLERM
RGELGDYGAGLKGHIRLLCNTAALSEYLPQALSRFLAKYPQISVDLEERPSQEIVDAVRAGLCDMGVVSDLVDLQGLQAF
KFRSDPLVLVVPEGHVLAGRRTIRLSEVLDCDFVGLVDGSALQEHVTQQARRLGGQLSYRVRLRSFEAVCRMVGLGIGVG
IVPRAAAARCARSARLKRVTLTDAWANRSLVLCLRDVDQLPLYARQMVQHVLAAESP

Sequences:

>Translated_297_residues
MRFDLTDLRLFLNVHEAGTITGGAGRSHMTLASASERIRSMEDTLGVPLLVRERRGVAVTAAGRTLLYHARVVLQQLERM
RGELGDYGAGLKGHIRLLCNTAALSEYLPQALSRFLAKYPQISVDLEERPSQEIVDAVRAGLCDMGVVSDLVDLQGLQAF
KFRSDPLVLVVPEGHVLAGRRTIRLSEVLDCDFVGLVDGSALQEHVTQQARRLGGQLSYRVRLRSFEAVCRMVGLGIGVG
IVPRAAAARCARSARLKRVTLTDAWANRSLVLCLRDVDQLPLYARQMVQHVLAAESP
>Mature_297_residues
MRFDLTDLRLFLNVHEAGTITGGAGRSHMTLASASERIRSMEDTLGVPLLVRERRGVAVTAAGRTLLYHARVVLQQLERM
RGELGDYGAGLKGHIRLLCNTAALSEYLPQALSRFLAKYPQISVDLEERPSQEIVDAVRAGLCDMGVVSDLVDLQGLQAF
KFRSDPLVLVVPEGHVLAGRRTIRLSEVLDCDFVGLVDGSALQEHVTQQARRLGGQLSYRVRLRSFEAVCRMVGLGIGVG
IVPRAAAARCARSARLKRVTLTDAWANRSLVLCLRDVDQLPLYARQMVQHVLAAESP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI145693105, Length=254, Percent_Identity=24.0157480314961, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI157672245, Length=268, Percent_Identity=26.865671641791, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787806, Length=273, Percent_Identity=24.1758241758242, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1790208, Length=257, Percent_Identity=26.0700389105058, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788887, Length=246, Percent_Identity=28.0487804878049, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1787879, Length=268, Percent_Identity=25.7462686567164, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1788748, Length=243, Percent_Identity=24.6913580246914, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790399, Length=246, Percent_Identity=23.5772357723577, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787530, Length=262, Percent_Identity=24.4274809160305, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1786713, Length=122, Percent_Identity=37.7049180327869, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788297, Length=259, Percent_Identity=25.0965250965251, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI2367136, Length=246, Percent_Identity=24.7967479674797, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789204, Length=239, Percent_Identity=23.4309623430962, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI1787601, Length=199, Percent_Identity=23.6180904522613, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789440, Length=121, Percent_Identity=28.099173553719, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32603; Mature: 32603

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFDLTDLRLFLNVHEAGTITGGAGRSHMTLASASERIRSMEDTLGVPLLVRERRGVAVT
CCCCHHHHHHEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEE
AAGRTLLYHARVVLQQLERMRGELGDYGAGLKGHIRLLCNTAALSEYLPQALSRFLAKYP
ECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHEEEHHHHHHHHHHHHHHHHHHHCC
QISVDLEERPSQEIVDAVRAGLCDMGVVSDLVDLQGLQAFKFRSDPLVLVVPEGHVLAGR
CEEEEHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHEEECCCCEEEEECCCEEEECC
RTIRLSEVLDCDFVGLVDGSALQEHVTQQARRLGGQLSYRVRLRSFEAVCRMVGLGIGVG
CEEEHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCC
IVPRAAAARCARSARLKRVTLTDAWANRSLVLCLRDVDQLPLYARQMVQHVLAAESP
CCCHHHHHHHHHHHHHEEEEEEHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRFDLTDLRLFLNVHEAGTITGGAGRSHMTLASASERIRSMEDTLGVPLLVRERRGVAVT
CCCCHHHHHHEEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEE
AAGRTLLYHARVVLQQLERMRGELGDYGAGLKGHIRLLCNTAALSEYLPQALSRFLAKYP
ECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHEEEHHHHHHHHHHHHHHHHHHHCC
QISVDLEERPSQEIVDAVRAGLCDMGVVSDLVDLQGLQAFKFRSDPLVLVVPEGHVLAGR
CEEEEHHCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCHHEEECCCCEEEEECCCEEEECC
RTIRLSEVLDCDFVGLVDGSALQEHVTQQARRLGGQLSYRVRLRSFEAVCRMVGLGIGVG
CEEEHHHHHCCCEEECCCCHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCCCC
IVPRAAAARCARSARLKRVTLTDAWANRSLVLCLRDVDQLPLYARQMVQHVLAAESP
CCCHHHHHHHHHHHHHEEEEEEHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]