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The map label for this gene is nusA [H]

Identifier: 91788290

GI number: 91788290

Start: 2525312

End: 2526832

Strand: Direct

Name: nusA [H]

Synonym: Bpro_2425

Alternate gene names: 91788290

Gene position: 2525312-2526832 (Clockwise)

Preceding gene: 91788289

Following gene: 91788291

Centisome position: 48.56

GC content: 59.37

Gene sequence:

>1521_bases
ATGAATCGCGAACTGTTGATGTTGGTTGATGCCATCTCGCGTGAGAAAAATGTTGAACGTGACGTCGTTTTTGGGGCTGT
CGAGCTTGCGCTCGCCTCGGCCACCAAGAAGGTGTACGAAGACGGCGTGGACATCCGCGTGGTGGTAGACCGTGACAGCG
GCAATTACGAGACTTTCCGCCGCTGGCTGGTGGTGCCTGATGAAGCCGGCCTGCAAAATCCGGAAGCCGAAGAGCTGGTG
AGCGACGCTCGCGAAGAAATTCCCGACATTGAAGAAGGCGACTACATCGAAAAACCGGTGGAGAGTCTGCCTATCGGTCG
CATCGGCGCACAAGCCGCCAAGCAGGTCATCCTGCAAAAAATCCGCGATGCCGAGCGCGAAATGCTGCTCAACGACTTCA
TGTCGCGCGGCGACAAGATTTTTGTCGGCACCGTCAAGCGCATGGACAAGGGCGACATCATTGTCGAATCTGGCCGTGTC
GAGGGGCGGCTGCGTCGCAGCGACATGATCCCCAAGGAAAATCTGCGTGCCGGAGACCGCGTCCGCGCCATGATCATGGA
GGTCGACACCACCCTGCGCGGTGCCCCCATCATCCTGTCGCGCACCGCCCCAGAGTACATGATCGAGCTGTTCCGCCAGG
AAGTGCCCGAGATCGAGCAGGGCCTGCTAGAGATCAAGTCCTGCGCCCGTGACCCCGGTTCGCGCGCCAAGATCGCCGTG
TTGTCGCACGACAAGCGGGTGGATCCCATCGGCACTTGTGTCGGGGTTCGCGGCACCCGTGTCAATGGCGTGACCAATGA
GCTCGCCGGCGAACGCGTCGATATCGTGCTGTGGAGCGAAGACCCGGCCCAGTTCGTGATTGGCGCACTGGCGCCTGCCA
ATGTGTCATCCATCGTGGTGGACGAAGAGCGCCACGCCATGGACGTCGTGGTCGACGAAGAAAACCTGGCCATTTCGATC
GGCCGCGGTGGCCAGAACGTGCGCCTGGCTGCAGAGCTGACCGGCTGGAAGATCAACATCATGACGGCCGACGAGTCGGC
CCAGAAGCAGGCGACCGAAACCGATACCAGCCGCAAGCTCTTCATGGAAAAGCTTGATGTGGACGAGGAAATCGCCGACA
TCCTGATCTCCGAAGGCTTCACCAGCCTGGAAGAAGTCGCGTATGTGCCGCTGCAGGAAATGCTCGAGATCGAATCGTTT
GATGAAGACACGGTCAATGAGTTGCGCACCCGCGCCAAAGATGCACTGTTGACGATGGAAATCGCCAAGGAAGAAAATGC
CGAAGGTGTTTCGCAGAACCTGCGTGACGTTGAAGGCATTACGCCCGAGCTGATCGCCAAACTTACCGAAGCGGGTGTCC
ATACCCGCGACGACCTGGCCGATCTGGCTGTGGACGAACTCACCGATATCACCGGCCAGTCTGCGGACGAAGCCAAGGCC
CTGATCATGACTGCTCGCGCCCATTGGTTTACCAATGACGCAAGTGACACCGCTGCACCTGCAACGGCCCAAGAGCAATG
A

Upstream 100 bases:

>100_bases
CGACTGGCGCCAGTGGTTGATTTCAAGGGCCGAAAACCCAAAATTGTCCCTGGTGCGGATAAATCTTCAGAGAAATAATT
TAGAAAGGCAGGCTTGTGAA

Downstream 100 bases:

>100_bases
TCATGGAGGCCAGCCAGGAAAACAAAATATGTCCAGTACCACTACCGTCGCTGAATTCGCAGCTGAATTGAATAAACCCA
CCGCCACCCTGATCGAGCAG

Product: NusA antitermination factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MNRELLMLVDAISREKNVERDVVFGAVELALASATKKVYEDGVDIRVVVDRDSGNYETFRRWLVVPDEAGLQNPEAEELV
SDAREEIPDIEEGDYIEKPVESLPIGRIGAQAAKQVILQKIRDAEREMLLNDFMSRGDKIFVGTVKRMDKGDIIVESGRV
EGRLRRSDMIPKENLRAGDRVRAMIMEVDTTLRGAPIILSRTAPEYMIELFRQEVPEIEQGLLEIKSCARDPGSRAKIAV
LSHDKRVDPIGTCVGVRGTRVNGVTNELAGERVDIVLWSEDPAQFVIGALAPANVSSIVVDEERHAMDVVVDEENLAISI
GRGGQNVRLAAELTGWKINIMTADESAQKQATETDTSRKLFMEKLDVDEEIADILISEGFTSLEEVAYVPLQEMLEIESF
DEDTVNELRTRAKDALLTMEIAKEENAEGVSQNLRDVEGITPELIAKLTEAGVHTRDDLADLAVDELTDITGQSADEAKA
LIMTARAHWFTNDASDTAAPATAQEQ

Sequences:

>Translated_506_residues
MNRELLMLVDAISREKNVERDVVFGAVELALASATKKVYEDGVDIRVVVDRDSGNYETFRRWLVVPDEAGLQNPEAEELV
SDAREEIPDIEEGDYIEKPVESLPIGRIGAQAAKQVILQKIRDAEREMLLNDFMSRGDKIFVGTVKRMDKGDIIVESGRV
EGRLRRSDMIPKENLRAGDRVRAMIMEVDTTLRGAPIILSRTAPEYMIELFRQEVPEIEQGLLEIKSCARDPGSRAKIAV
LSHDKRVDPIGTCVGVRGTRVNGVTNELAGERVDIVLWSEDPAQFVIGALAPANVSSIVVDEERHAMDVVVDEENLAISI
GRGGQNVRLAAELTGWKINIMTADESAQKQATETDTSRKLFMEKLDVDEEIADILISEGFTSLEEVAYVPLQEMLEIESF
DEDTVNELRTRAKDALLTMEIAKEENAEGVSQNLRDVEGITPELIAKLTEAGVHTRDDLADLAVDELTDITGQSADEAKA
LIMTARAHWFTNDASDTAAPATAQEQ
>Mature_506_residues
MNRELLMLVDAISREKNVERDVVFGAVELALASATKKVYEDGVDIRVVVDRDSGNYETFRRWLVVPDEAGLQNPEAEELV
SDAREEIPDIEEGDYIEKPVESLPIGRIGAQAAKQVILQKIRDAEREMLLNDFMSRGDKIFVGTVKRMDKGDIIVESGRV
EGRLRRSDMIPKENLRAGDRVRAMIMEVDTTLRGAPIILSRTAPEYMIELFRQEVPEIEQGLLEIKSCARDPGSRAKIAV
LSHDKRVDPIGTCVGVRGTRVNGVTNELAGERVDIVLWSEDPAQFVIGALAPANVSSIVVDEERHAMDVVVDEENLAISI
GRGGQNVRLAAELTGWKINIMTADESAQKQATETDTSRKLFMEKLDVDEEIADILISEGFTSLEEVAYVPLQEMLEIESF
DEDTVNELRTRAKDALLTMEIAKEENAEGVSQNLRDVEGITPELIAKLTEAGVHTRDDLADLAVDELTDITGQSADEAKA
LIMTARAHWFTNDASDTAAPATAQEQ

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=498, Percent_Identity=49.3975903614458, Blast_Score=479, Evalue=1e-136,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR003583
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 55982; Mature: 55982

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRELLMLVDAISREKNVERDVVFGAVELALASATKKVYEDGVDIRVVVDRDSGNYETFR
CCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
RWLVVPDEAGLQNPEAEELVSDAREEIPDIEEGDYIEKPVESLPIGRIGAQAAKQVILQK
EEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
IRDAEREMLLNDFMSRGDKIFVGTVKRMDKGDIIVESGRVEGRLRRSDMIPKENLRAGDR
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCHHHHHCCCCHHHCCCCHH
VRAMIMEVDTTLRGAPIILSRTAPEYMIELFRQEVPEIEQGLLEIKSCARDPGSRAKIAV
HHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEE
LSHDKRVDPIGTCVGVRGTRVNGVTNELAGERVDIVLWSEDPAQFVIGALAPANVSSIVV
EECCCCCCHHHHEECCCCCEECCCHHHHCCCEEEEEEECCCCHHHHHHHCCCCCCCEEEE
DEERHAMDVVVDEENLAISIGRGGQNVRLAAELTGWKINIMTADESAQKQATETDTSRKL
ECCCCCEEEEEECCCEEEEECCCCCCEEEEEEECCEEEEEEECCCHHHHHHHHCHHHHHH
FMEKLDVDEEIADILISEGFTSLEEVAYVPLQEMLEIESFDEDTVNELRTRAKDALLTME
HHHHCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
IAKEENAEGVSQNLRDVEGITPELIAKLTEAGVHTRDDLADLAVDELTDITGQSADEAKA
HHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
LIMTARAHWFTNDASDTAAPATAQEQ
HEEEHHHHEECCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNRELLMLVDAISREKNVERDVVFGAVELALASATKKVYEDGVDIRVVVDRDSGNYETFR
CCCCEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHH
RWLVVPDEAGLQNPEAEELVSDAREEIPDIEEGDYIEKPVESLPIGRIGAQAAKQVILQK
EEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHH
IRDAEREMLLNDFMSRGDKIFVGTVKRMDKGDIIVESGRVEGRLRRSDMIPKENLRAGDR
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCCCCCHHHHHCCCCHHHCCCCHH
VRAMIMEVDTTLRGAPIILSRTAPEYMIELFRQEVPEIEQGLLEIKSCARDPGSRAKIAV
HHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCEEEE
LSHDKRVDPIGTCVGVRGTRVNGVTNELAGERVDIVLWSEDPAQFVIGALAPANVSSIVV
EECCCCCCHHHHEECCCCCEECCCHHHHCCCEEEEEEECCCCHHHHHHHCCCCCCCEEEE
DEERHAMDVVVDEENLAISIGRGGQNVRLAAELTGWKINIMTADESAQKQATETDTSRKL
ECCCCCEEEEEECCCEEEEECCCCCCEEEEEEECCEEEEEEECCCHHHHHHHHCHHHHHH
FMEKLDVDEEIADILISEGFTSLEEVAYVPLQEMLEIESFDEDTVNELRTRAKDALLTME
HHHHCCCHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
IAKEENAEGVSQNLRDVEGITPELIAKLTEAGVHTRDDLADLAVDELTDITGQSADEAKA
HHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCHHHHH
LIMTARAHWFTNDASDTAAPATAQEQ
HEEEHHHHEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12704232 [H]