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The map label for this gene is iscS [H]

Identifier: 91788051

GI number: 91788051

Start: 2274360

End: 2275577

Strand: Direct

Name: iscS [H]

Synonym: Bpro_2178

Alternate gene names: 91788051

Gene position: 2274360-2275577 (Clockwise)

Preceding gene: 91788050

Following gene: 91788052

Centisome position: 43.74

GC content: 60.26

Gene sequence:

>1218_bases
ATGGATACCCCACACTTTCCGATTTATATGGATTACAGCGCCACCAATCCTTGCGACCCGAGGGTGGTGGATGCCATGAT
TCCCTGGTTGCGCGAGCATTTCGGCAACCCGGCTTCCCGCAGCCATGCCTGGGGCTGGGAGGCAGAAGCTGCGGTTGAAA
ATGCGCGCGAGCAGGTTGCCGCACTGATTGGCGCCGATCCGCGCGAAATCGTCTGGACCAGCGGTGCGACCGAATCCAAC
AATCTGGCGCTGAAAGGCGCCGCCCATTTTTACAAGACCAAGGGCAAGCACCTCATCACGGTCAAGACTGAGCACAAGGC
TGTGCTTGATACCTGCCGCGAACTGGAACGCCAGGGTTTTGAAGTCACCTATTTGGATGTGCAGGCCGATGGCCTGCTTG
ACATGGAGGCCCTCAAGGCCGCCATGCGGCCCGACACCATTGTGGTCAGCGTCATGTACGTCAACAACGAAATTGGCGTG
ATCCAGGACATCCCCGCGATTGGCGCCCTGTGCCGCGAGCGGGGCATTATTTTCCATGTGGACGCTGCGCAGGCGACCGG
CCGTGTGGACATCGACCTGGCGACACTGCCGGTGGACCTGATGAGCTTGACTTCGCACAAAACCTACGGCCCCAAAGGCA
TTGGCGCGCTGTATGTGCGCCGCAAGCCCCGCGTGCGCCTGGAGGCCCAGATGCACGGCGGAGGCCACGAGCGCGGCATG
CGCTCCGGTACCTTGCCTACGCATCAGTGCGTGGGCATGGGCGAGGCCTACCGCATTGCCAAAGCAGAGATGCACGAAGA
GAACAAGCGTATCGCCGCCCTGCACGAGCGCATGCTCAACGGCCTCAAGGATGTGGAGGAAGTGTTCCTCAATGGCCATG
CTGAAAAGCGCGTGCCGCACAACCTGAACATGAGCTTCAATTTCGTCGAGGGCGAGTCGCTGATCATGGGTATCAAGGGC
CTGGCTGTCTCCAGCGGTTCTGCCTGTACCTCGGCCAGTCTGGAGCCCAGTTATGTATTGCGCGCGTTGGGTCGCAGCGA
CGAACTGGCGCACAGCAGCCTGCGCATGACGATCGGCCGCTGGAGCACGGAAGAAGAGATCGATTACGCGGTTGAAACCA
TCAAGGAAAATGTGGCCAAGCTGCGTGAACTGTCCCCGCTGTGGGAGATGTTCAAGGATGGGGTTGACCTGTCCACCATC
CAGTGGGCTGCCCACTAG

Upstream 100 bases:

>100_bases
GCCTGAACGCAGTATTTTGCCGGGTTCGACCTGCGGATGAATCGAGGTCGTTGTTTTGAGCTGCCAGCCAACCTGTGAAC
TAAAAAAGCTAGCTAAATAA

Downstream 100 bases:

>100_bases
ACCGGCAGATCCAGCAGATCAGCAGACAAGACAGCTTTAAGAGGTAAACAAAATGGCATACAGCGAAAAAGTGGTAGACC
ACTATGAAAATCCCCGCAAT

Product: cysteine desulfurase IscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MDTPHFPIYMDYSATNPCDPRVVDAMIPWLREHFGNPASRSHAWGWEAEAAVENAREQVAALIGADPREIVWTSGATESN
NLALKGAAHFYKTKGKHLITVKTEHKAVLDTCRELERQGFEVTYLDVQADGLLDMEALKAAMRPDTIVVSVMYVNNEIGV
IQDIPAIGALCRERGIIFHVDAAQATGRVDIDLATLPVDLMSLTSHKTYGPKGIGALYVRRKPRVRLEAQMHGGGHERGM
RSGTLPTHQCVGMGEAYRIAKAEMHEENKRIAALHERMLNGLKDVEEVFLNGHAEKRVPHNLNMSFNFVEGESLIMGIKG
LAVSSGSACTSASLEPSYVLRALGRSDELAHSSLRMTIGRWSTEEEIDYAVETIKENVAKLRELSPLWEMFKDGVDLSTI
QWAAH

Sequences:

>Translated_405_residues
MDTPHFPIYMDYSATNPCDPRVVDAMIPWLREHFGNPASRSHAWGWEAEAAVENAREQVAALIGADPREIVWTSGATESN
NLALKGAAHFYKTKGKHLITVKTEHKAVLDTCRELERQGFEVTYLDVQADGLLDMEALKAAMRPDTIVVSVMYVNNEIGV
IQDIPAIGALCRERGIIFHVDAAQATGRVDIDLATLPVDLMSLTSHKTYGPKGIGALYVRRKPRVRLEAQMHGGGHERGM
RSGTLPTHQCVGMGEAYRIAKAEMHEENKRIAALHERMLNGLKDVEEVFLNGHAEKRVPHNLNMSFNFVEGESLIMGIKG
LAVSSGSACTSASLEPSYVLRALGRSDELAHSSLRMTIGRWSTEEEIDYAVETIKENVAKLRELSPLWEMFKDGVDLSTI
QWAAH
>Mature_405_residues
MDTPHFPIYMDYSATNPCDPRVVDAMIPWLREHFGNPASRSHAWGWEAEAAVENAREQVAALIGADPREIVWTSGATESN
NLALKGAAHFYKTKGKHLITVKTEHKAVLDTCRELERQGFEVTYLDVQADGLLDMEALKAAMRPDTIVVSVMYVNNEIGV
IQDIPAIGALCRERGIIFHVDAAQATGRVDIDLATLPVDLMSLTSHKTYGPKGIGALYVRRKPRVRLEAQMHGGGHERGM
RSGTLPTHQCVGMGEAYRIAKAEMHEENKRIAALHERMLNGLKDVEEVFLNGHAEKRVPHNLNMSFNFVEGESLIMGIKG
LAVSSGSACTSASLEPSYVLRALGRSDELAHSSLRMTIGRWSTEEEIDYAVETIKENVAKLRELSPLWEMFKDGVDLSTI
QWAAH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=400, Percent_Identity=59.25, Blast_Score=523, Evalue=1e-148,
Organism=Homo sapiens, GI156713448, Length=420, Percent_Identity=33.3333333333333, Blast_Score=201, Evalue=1e-51,
Organism=Escherichia coli, GI48994898, Length=402, Percent_Identity=69.1542288557214, Blast_Score=597, Evalue=1e-172,
Organism=Escherichia coli, GI1789175, Length=213, Percent_Identity=30.9859154929577, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1787970, Length=390, Percent_Identity=23.8461538461538, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25143064, Length=401, Percent_Identity=56.857855361596, Blast_Score=493, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17533177, Length=327, Percent_Identity=29.3577981651376, Blast_Score=131, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6319831, Length=399, Percent_Identity=56.390977443609, Blast_Score=486, Evalue=1e-138,
Organism=Drosophila melanogaster, GI20129463, Length=400, Percent_Identity=57, Blast_Score=488, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 44866; Mature: 44866

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTPHFPIYMDYSATNPCDPRVVDAMIPWLREHFGNPASRSHAWGWEAEAAVENAREQVA
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALIGADPREIVWTSGATESNNLALKGAAHFYKTKGKHLITVKTEHKAVLDTCRELERQGF
HHHCCCCHHEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCC
EVTYLDVQADGLLDMEALKAAMRPDTIVVSVMYVNNEIGVIQDIPAIGALCRERGIIFHV
EEEEEEEECCCCCCHHHHHHHCCCCEEEEEEEEECCCCCCHHCCHHHHHHHHHCCCEEEE
DAAQATGRVDIDLATLPVDLMSLTSHKTYGPKGIGALYVRRKPRVRLEAQMHGGGHERGM
ECCCCCCEEEEEEEECCHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHCCC
RSGTLPTHQCVGMGEAYRIAKAEMHEENKRIAALHERMLNGLKDVEEVFLNGHAEKRVPH
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
NLNMSFNFVEGESLIMGIKGLAVSSGSACTSASLEPSYVLRALGRSDELAHSSLRMTIGR
CCCCEEEEECCCEEEEEEECEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHEEECC
WSTEEEIDYAVETIKENVAKLRELSPLWEMFKDGVDLSTIQWAAH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC
>Mature Secondary Structure
MDTPHFPIYMDYSATNPCDPRVVDAMIPWLREHFGNPASRSHAWGWEAEAAVENAREQVA
CCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
ALIGADPREIVWTSGATESNNLALKGAAHFYKTKGKHLITVKTEHKAVLDTCRELERQGF
HHHCCCCHHEEEECCCCCCCCEEEEEHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCC
EVTYLDVQADGLLDMEALKAAMRPDTIVVSVMYVNNEIGVIQDIPAIGALCRERGIIFHV
EEEEEEEECCCCCCHHHHHHHCCCCEEEEEEEEECCCCCCHHCCHHHHHHHHHCCCEEEE
DAAQATGRVDIDLATLPVDLMSLTSHKTYGPKGIGALYVRRKPRVRLEAQMHGGGHERGM
ECCCCCCEEEEEEEECCHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEEECCCCHHCCC
RSGTLPTHQCVGMGEAYRIAKAEMHEENKRIAALHERMLNGLKDVEEVFLNGHAEKRVPH
CCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
NLNMSFNFVEGESLIMGIKGLAVSSGSACTSASLEPSYVLRALGRSDELAHSSLRMTIGR
CCCCEEEEECCCEEEEEEECEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHEEECC
WSTEEEIDYAVETIKENVAKLRELSPLWEMFKDGVDLSTIQWAAH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA