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The map label for this gene is dusA [H]

Identifier: 91788026

GI number: 91788026

Start: 2244723

End: 2245757

Strand: Direct

Name: dusA [H]

Synonym: Bpro_2153

Alternate gene names: 91788026

Gene position: 2244723-2245757 (Clockwise)

Preceding gene: 91788022

Following gene: 91788028

Centisome position: 43.17

GC content: 65.22

Gene sequence:

>1035_bases
ATGTCAACGCCCGTTCCCGGCTCAGCCGGCTCTTTGCATTCTGTCACCGATAACCGTGCGCATTCGCCGTGGCGCCTGAG
CGTGGCCCCGATGATGGACTGGACGGACCGGCATTGCCGCCACTTTCACCGCCTGCTGTCTGGCCACGCCTTGCTGTATA
CCGAGATGGTGACAACCGGTGCCCTGATACATGGCGACGTGGCGCGGCATCTTCGTTTTGGCGTGGAGGAGCACCCGGTG
GCCCTGCAACTGGGCGGCAGCGAGCCGGCCGAACTGGCCCAGTGCGCCACGCTGGGCGAGCAGTGGGGCTATGACGAGAT
CAACCTGAACTGCGGCTGCCCCAGCGAGCGGGTGCAGCGCGGCTCCTTCGGCGCCTGCCTGATGGCCGAGCCGCAACTGG
TGGCCGATTGCGTCAAGGCCATGGTCGACGTGGTGTCCGTTCCTGTGACGGTGAAGCACCGTATCGGCATCGACAAGGGC
GAAAGCTACGGTTTTGTGCGTGATTTTGTGGGCGCGGTCAGCGAGGCGGGGTGCAGCACCTTCATCGTCCACGCGCGCAA
CGCCTGGCTCAAGGGCCTTTCCCCGAAGGAAAACCGCGAGGTGCCGCCGCTGCGCTATGAACTGGTGCACCGGCTCAAGG
CCGAGTTTCCGCAACTCACCATCGCCATCAACGGCGGCATCACTACCAGCACGCAAGTGGCCGAGCAGCTGCAGCAACTC
GACGGCGTGATGATTGGCCGCGAGGCTTATCACAACCCCTGGTGGCTCAGCGTTTGGGATGCGGCGTTTTACGGTGTGGC
GGATGCTGGGCGGACGCGCGAATCCGTTGAAGCGCAGATGGTGGACTACATGGCACGCGAAGCCGCCGAGCATGGCACGC
CCTGGAGCAGCATTGCACGTCACATGCTGGGTTTGCGCCACGGCCTGCCTGGGGCGCGGCGCTGGCGGCAGGTCTGGAGC
AACCACAAGCTCAAGGCCCTTCACCCGCGCGACGTGATGGCGCTGGCGCATGAGGCTGTGCTGGAGGCGGCATAG

Upstream 100 bases:

>100_bases
GTTACCCGGCTTGTGTGGCGGCTTGCCTTGGTGACTTCCCCCTGGCTGCCCATGGGCCAAGAGAAACACTGGGTTTTACT
CTGCTGGGATAATTGGACTC

Downstream 100 bases:

>100_bases
GGCGGCTGCCTCGGGGCGCGTCCGGGCTACCCGGGCTATAGGGCTATAGGGCTATAGGCCGACGGCGCTGCGCCGGTCAA
TCTTGCGGCTAAGGACGATG

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MSTPVPGSAGSLHSVTDNRAHSPWRLSVAPMMDWTDRHCRHFHRLLSGHALLYTEMVTTGALIHGDVARHLRFGVEEHPV
ALQLGGSEPAELAQCATLGEQWGYDEINLNCGCPSERVQRGSFGACLMAEPQLVADCVKAMVDVVSVPVTVKHRIGIDKG
ESYGFVRDFVGAVSEAGCSTFIVHARNAWLKGLSPKENREVPPLRYELVHRLKAEFPQLTIAINGGITTSTQVAEQLQQL
DGVMIGREAYHNPWWLSVWDAAFYGVADAGRTRESVEAQMVDYMAREAAEHGTPWSSIARHMLGLRHGLPGARRWRQVWS
NHKLKALHPRDVMALAHEAVLEAA

Sequences:

>Translated_344_residues
MSTPVPGSAGSLHSVTDNRAHSPWRLSVAPMMDWTDRHCRHFHRLLSGHALLYTEMVTTGALIHGDVARHLRFGVEEHPV
ALQLGGSEPAELAQCATLGEQWGYDEINLNCGCPSERVQRGSFGACLMAEPQLVADCVKAMVDVVSVPVTVKHRIGIDKG
ESYGFVRDFVGAVSEAGCSTFIVHARNAWLKGLSPKENREVPPLRYELVHRLKAEFPQLTIAINGGITTSTQVAEQLQQL
DGVMIGREAYHNPWWLSVWDAAFYGVADAGRTRESVEAQMVDYMAREAAEHGTPWSSIARHMLGLRHGLPGARRWRQVWS
NHKLKALHPRDVMALAHEAVLEAA
>Mature_343_residues
STPVPGSAGSLHSVTDNRAHSPWRLSVAPMMDWTDRHCRHFHRLLSGHALLYTEMVTTGALIHGDVARHLRFGVEEHPVA
LQLGGSEPAELAQCATLGEQWGYDEINLNCGCPSERVQRGSFGACLMAEPQLVADCVKAMVDVVSVPVTVKHRIGIDKGE
SYGFVRDFVGAVSEAGCSTFIVHARNAWLKGLSPKENREVPPLRYELVHRLKAEFPQLTIAINGGITTSTQVAEQLQQLD
GVMIGREAYHNPWWLSVWDAAFYGVADAGRTRESVEAQMVDYMAREAAEHGTPWSSIARHMLGLRHGLPGARRWRQVWSN
HKLKALHPRDVMALAHEAVLEAA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=300, Percent_Identity=26.3333333333333, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI40807366, Length=294, Percent_Identity=27.5510204081633, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI145693211, Length=320, Percent_Identity=59.0625, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1789660, Length=234, Percent_Identity=27.7777777777778, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788462, Length=241, Percent_Identity=28.2157676348548, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI25144369, Length=258, Percent_Identity=25.968992248062, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=304, Percent_Identity=24.6710526315789, Blast_Score=73, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24580595, Length=304, Percent_Identity=25.6578947368421, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19920448, Length=304, Percent_Identity=25.6578947368421, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37997; Mature: 37866

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPVPGSAGSLHSVTDNRAHSPWRLSVAPMMDWTDRHCRHFHRLLSGHALLYTEMVTTG
CCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHC
ALIHGDVARHLRFGVEEHPVALQLGGSEPAELAQCATLGEQWGYDEINLNCGCPSERVQR
HHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCC
GSFGACLMAEPQLVADCVKAMVDVVSVPVTVKHRIGIDKGESYGFVRDFVGAVSEAGCST
CCCCEEEECCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCE
FIVHARNAWLKGLSPKENREVPPLRYELVHRLKAEFPQLTIAINGGITTSTQVAEQLQQL
EEEECHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
DGVMIGREAYHNPWWLSVWDAAFYGVADAGRTRESVEAQMVDYMAREAAEHGTPWSSIAR
CCEEECHHHHCCCCEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
HMLGLRHGLPGARRWRQVWSNHKLKALHPRDVMALAHEAVLEAA
HHHHHHHCCCHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STPVPGSAGSLHSVTDNRAHSPWRLSVAPMMDWTDRHCRHFHRLLSGHALLYTEMVTTG
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHC
ALIHGDVARHLRFGVEEHPVALQLGGSEPAELAQCATLGEQWGYDEINLNCGCPSERVQR
HHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHCC
GSFGACLMAEPQLVADCVKAMVDVVSVPVTVKHRIGIDKGESYGFVRDFVGAVSEAGCST
CCCCEEEECCHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCE
FIVHARNAWLKGLSPKENREVPPLRYELVHRLKAEFPQLTIAINGGITTSTQVAEQLQQL
EEEECHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHH
DGVMIGREAYHNPWWLSVWDAAFYGVADAGRTRESVEAQMVDYMAREAAEHGTPWSSIAR
CCEEECHHHHCCCCEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
HMLGLRHGLPGARRWRQVWSNHKLKALHPRDVMALAHEAVLEAA
HHHHHHHCCCHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]